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Peer Reviewed
See detailInvestigation of airway reactivity by barometric whole body plethysmography in cats with spontaneous bronchial disease
Bernaerts, Frederique ULg; Merveille, Anne-Christine ULg; Bolognin, M. et al

in 24th Symposium of the Veterinary Comarative Respiratory Society - Jena, Allemagne 8-10 octobre 2006 (2006, October)

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See detailInvestigation of anaerobic digestion in a two-stage bioprocess producing hydrogen and methane
Hiligsmann, Serge ULg; Hamilton, Christopher ULg; Beckers, Laurent ULg et al

Conference (2010, November 17)

Hydrogen has received wide attention in the last decades as a clean energy vector. The major advantage of energy generation from hydrogen is the near-zero carbon emissions, since the utilization of ... [more ▼]

Hydrogen has received wide attention in the last decades as a clean energy vector. The major advantage of energy generation from hydrogen is the near-zero carbon emissions, since the utilization of hydrogen, either via combustion or via fuel cells, results in pure water. Recently, there has been increasing interest on the biological production of hydrogen gas from renewable biomass such as carbohydrates from agriculture or agro-food industries. This specific anaerobic digestion is called dark fermentation and is involved in the classic anaerobic digestion producing methane. Indeed, in a two-step process, i.e. when acido- and aceto-genesis are carried out in a different bioreactor than methanogenesis, it is feasible to generate separated biogas flows containing either H2 or CH4 depending on specific operating parameters. This paper deals with the comparison of the first stage performances whether operated in optimum conditions for H2 production with a mixed culture or a pure Clostridium butyricum strain. Hydrogen yields of about 1.75 and 2.3 mol H2/mol glucose were achieved respectively. The metabolites, mainly acetate and butyrate, contained in the spent medium were efficiently converted to methane in a second anaerobic digester with a methane yield of about 170 ml/g COD initially fed in the first stage. [less ▲]

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See detailInvestigation of application sharing systems for teaching purposes in engineering disciplines
Burger, C.; Papakosta, S.; Bullinger, Eric ULg et al

in 4th Int. Conf. New Educational Environment (2002)

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See detailInvestigation of automatic memory during general anesthesia for elective surgery using the process dissociation procedure
Willems, Sylvie ULg; Iselin-Chaves, Irène A.; Jermann, Françoise J. et al

in Anesthesiology (2005), 103

Background: This prospective study evaluated memory function during general anesthesia for elective surgery and its relation to depth of hypnotic state. The authors also compared memory function in ... [more ▼]

Background: This prospective study evaluated memory function during general anesthesia for elective surgery and its relation to depth of hypnotic state. The authors also compared memory function in anesthetized and nonanesthetized subjects. Methods: Words were played for 70 min via headphones to 48 patients (aged 18–70 yr) after induction of general anesthesia for elective surgery. Patients were unpremedicated, and the anesthetic regimen was free. The Bispectral Index (BIS) was recorded throughout the study. Within 36 h after the word presentation, memory was assessed using an auditory word stem completion test with inclusion and exclusion instructions. Memory performance and the contribution of explicit and implicit memory were calculated using the process dissociation procedure. The authors applied the same memory task to a control group of nonanesthetized subjects. Results: Forty-seven patients received isoflurane, and one patient received propofol for anesthesia. The mean ( SD) BIS was 49 9. There was evidence of memory for words presented during light (BIS 61–80) and adequate anesthesia (BIS 41–60) but not during deep anesthesia (BIS 21–40). The process dissociation procedure showed a significant implicit memory contribution but not reliable explicit memory contribution (mean explicit memory scores 0.05 0.14, 0.04 0.09, and 0.05 0.14; mean automatic influence scores 0.14 0.12, 0.17 0.17, and 0.18 0.21 at BIS 21–40, 41–60, and 61–80, respectively). Compared with anesthetized patients, the memory performance of nonanesthetized subjects was better, with a higher contribution by explicit memory and a comparable contribution by implicit memory. Conclusion: During general anesthesia for elective surgery, implicit memory persists even in adequate hypnotic states, to a comparable degree as in nonanesthetized subjects. [less ▲]

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See detailInvestigation of biological markers to evaluate the adaptation of the newborn Belgian Blue calf to the extrauterine life. A pilot study.
Ronzoni, Anna ULg; Theron, Léonard ULg; De Marchin, Emmanuelle et al

in Dalin, Göran (Ed.) 15th International Conference on Production Diseases in Farms Animals, Book of Abstracts (2013, June)

Adaptation to extrauterine life and immunity transfer at calving are more at stake when dealing with highly productive breeds such as the Belgian Blue Cattle Breed (BBCB). The purpose of the present pilot ... [more ▼]

Adaptation to extrauterine life and immunity transfer at calving are more at stake when dealing with highly productive breeds such as the Belgian Blue Cattle Breed (BBCB). The purpose of the present pilot study was to evaluate different blood markers, from the birth to the first week of life in BBCB calves, in order to investigate fundamental aspects of the adaptation to the extrauterine life as passive immunity transfer and early energy metabolism. Eighty calves coming from 10 different farms located in Wallonia (Belgium) were enrolled. A clinical examination was performed to verify viability and maturity at birth. Blood samples were collected at birth, day 1 and day 3 to 7. Passive immunity transfer analysis included serum and colostrum immunoglobulin G (IgG) concentration, and plasmatic selenium concentration (Se). Glycemia, serum insulin and cortisol concentration have been considered for the energy metabolism analysis. The data collected were statistically analysed using logistic regression and unpaired t-test. All calves were born alive, viable and mature by caesarean section. Mortality rate was 0% at one week and 5% at three months. Only 33% of calves had an IgG concentration >16g/L after one week of age, while only 34% of colostra had an IgG concentration >70g/L. Glycemia and serum insulin concentration at birth were significantly lower than other breeds, and plasmatic selenium concentration were coherent with low supplemented dams. We compared calves across IgG concentration classes (>16 g/L vs <16g/L). The crosssectional analysis of the risk of FPT when given low IgG concentration colostra has shown a non significant odd ratio (OR 1,058 and 0,39116 g/L) (p<0,001). No significant difference has been shown between cortisol and glycemia (p=0,3) nor insulin and glycemia (p=0,2) at birth. These data suggest a significant difference in BBCB calves on several metabolic markers, but they also revealed that immunity transfer and micronutrition of pregnant hyper-productive dams remain a challenge in modern farms. [less ▲]

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses : effect of discipline and gender.
Kirschvink, N.; De Moffarts, Brieuc; Farnir, Frédéric ULg et al

in 7th International Conference on Equine Exercise Physiology (2006)

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds
Kirschvink, Nathalie; de Moffarts, Brieuc; Farnir, Frédéric ULg et al

in Equine Veterinary Journal. Supplement (2006), 36

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently ... [more ▼]

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age. OBJECTIVES: To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses. METHODS: Healthy horses (n = 493) underwent oxidant/ antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant. RESULTS: Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2-6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E. CONCLUSIONS: Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E. POTENTIAL RELEVANCE: The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses. [less ▲]

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See detailInvestigation of body condition score and insulin sensitivity (RQUICKY) in downer cow syndrome
Bonvoisin, Marie ULg; Rollin, Frédéric ULg; Lebreton, Pascal et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailInvestigation of carbohydrate binding property of a fungal lectin from Xerocomus chrysenteron and potential use on Myzus persicae aphid.
Jaber, Karimi; Cuartero Diaz, Gaetan; Haubruge, Eric ULg et al

in Communications in Agricultural and Applied Biological Sciences (2008), 73(3), 629-38

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice ... [more ▼]

In recent years encoding insecticidal Lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice, tobacco and potatoes. Xerocomus chrysenteron Lectin (XCL) has a high hemagglutinating activity and results obtained from sugar specificity assay showed to have specific affinity to Galactose and N-Acetylgalactosamine (GalNAc). In previous studies, XCL was shown to have negative effects on some insect pests, including aphids. In the present study, the effects of different carbohydrates including D-glucose, D-mannose, D-galactose and GalNAc, associated with 0.1% XCL (w/v) in artificial diet was investigated to assess the evolution of the lectin toxicity toward Myzus persicae aphid during 7 days. M. persicae, a polyphagous aphid, showed no significant differences of mortality when fed with the XCL lectin associated with Glucose and Mannose or fed on XCL diet only. At the opposite, the mortality rates related to artificial diet supplemented with Galactose or GalNAc and XCL were significantly reduced. There was then a significant mortality difference between M. persicae fed on an artificial diet incorporated specific carbohydrate binding Lectin with those fed with lectin only. The potential use of this particular fungal Lectin (XCL) with more specific carbohydrate binding will be discussed in relation to the development of bio-insecticide and integrated pest management. [less ▲]

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See detailInvestigation of cytokine expression in canine idiopathic pulmonary fibrosis
Krafft, Emilie ULg; Heikkilä, HP; Vanherberghen, Morgane ULg et al

in Proceeding of 29th VCRS Symposium (2011, November 02)

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See detailInvestigation of defect levels in Mg-doped GaN Schottky structures by thermal admittance spectroscopy
Nguyen, Ngoc Duy ULg; Germain, Marianne; Schmeits, Marcel et al

in Physica Status Solidi B. Basic Research (2001), 228

Schottky structures based on Mg-doped GaN layers grown by metalorganic chemical vapor deposition (MOCVD) on sapphire substrate are studied by thermal admittance spectroscopy from 90 K to room temperature ... [more ▼]

Schottky structures based on Mg-doped GaN layers grown by metalorganic chemical vapor deposition (MOCVD) on sapphire substrate are studied by thermal admittance spectroscopy from 90 K to room temperature. Evidence of two impurity levels results from the analysis of the observed peaks in the conductance curves, whose positions and strengths are temperature dependent. The experimental results are analyzed within a detailed theoretical study of the steady-state and small-signal electrical characteristics of the structure. Numerical simulations are based on the solution of the basic semiconductor equations for the structure consisting of two Schottky diodes connected back-to-back by a conduction channel formed by the GaN layer. [less ▲]

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See detailInvestigation of defect levels in Mg-doped GaN Schottky structures by thermal admittance spectroscopy
Nguyen, Ngoc Duy ULg; Germain, Marianne; Schmeits, Marcel et al

in Ponce, Fernando; Bell, Abigail (Eds.) ICNS-4: Proceedings of The Fourth International Conference on Nitride Semiconductors (2001)

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See detailInvestigation of differential virus transmission efficiency in aphid strains: from biological to proteomic assays
Bosquée, Emilie ULg; Francis, Frédéric ULg

Poster (2014, June)

Aphids are well known for their variable efficiency in virus transmission to host plant. Not only aphid species but also aphid strain is an important factor to consider in the plant-aphid-virus ... [more ▼]

Aphids are well known for their variable efficiency in virus transmission to host plant. Not only aphid species but also aphid strain is an important factor to consider in the plant-aphid-virus interactions. In order to investigate these relations, different approaches should be developped from biological observations assessing the virus transmission efficiency to molecular tasks to better know the involved mechanisms in the aphid vector. After a period of virus acquisition on an artificial diet (depending on the kind of transmission, permanent or non permanent ways), the virus transmission rate were assayed and followed by the determination of the presence of virus in the aphid by PCR or immudetection. The aphids were then collected to investigate the variation of aphid proteome and potential involvement in virus interactions. Changes in protein expressions were investigated using a 2D-DIGE approach comparing most and least efficient aphid strains selecting different aphid – virus models (PVY and PLRV in potato, BYDV in wheat, CMV on tobacco) . The proteome changes were analysed using the samespot software. Protein spots with significant up- and down expressions were then mechanically picked, trypsin digested and analysed by Maldi-Tof-Tof. Significantly changed proteins were classify according to metablic pathways and biological functions. Interesting proteins found to probably interact with virus were not only detected from aphid but also from related bacterial symbionts. Theses results were discussed in order to better understand virus – vector relations and potentially promote new ways to control virus transmitted by hemipteran pest. [less ▲]

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See detailInvestigation of DyBa2CU3O7-d superconducting domains grown by the infiltration technique starting with small size Dy-211 particles
Mathieu, Jean-Philippe; Koutzarova, T.; Rulmont, André ULg et al

in Superconductor Science and Technology (2005), 18(2 Sp. Iss. SI), 136-141

An infiltration and growth process is here used as an alternative to the classical top-seeded melt-textured growth process for the production of Dy-123 single-domains with finely dispersed small size Dy ... [more ▼]

An infiltration and growth process is here used as an alternative to the classical top-seeded melt-textured growth process for the production of Dy-123 single-domains with finely dispersed small size Dy-211 particles. The starting materials are the 211-particles and a barium and copper rich liquid phase precursor. The infiltration and growth process allows for controlling both the spatial and size distribution of the 211-particles in the final superconducting 123-single-domain. The main parameters (set-ups, maximum processing temperature with respect to the peritectic temperature, nature of reactant, porosity of the 211-preform) of the infiltration and growth process are discussed. Moreover, different processes of chimie douce are shown in order to produce Dy-211 particles with controlled shape and size, particles that can be used as precursors for the infiltration and growth process. [less ▲]

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See detailInvestigation of edge effect on wood density in recent tropical forest edges in Yangambi Man and Biosphere Reserve
de Haulleville, Thalès ULg; Bogaert, Jan ULg; Beeckman, Hans

Conference (2013, April)

Rapid development of lumbering activities in tropical forested areas has an important impact on carbon stocks and landscape configuration. Indeed, lumbering tend to fragment forest patches, extend edges ... [more ▼]

Rapid development of lumbering activities in tropical forested areas has an important impact on carbon stocks and landscape configuration. Indeed, lumbering tend to fragment forest patches, extend edges length, and expose core areas to non-forested ecosystems. These exposed core areas then undergo new constraints that can lead to a shift in their ecological features, called the edge effect. We investigate the possible presence of an edge effect on wood density in Yangambi reserve. Wood density (along with diameter and height) is a key feature for biomass estimations in tropical forests. Thus, fluctuations of wood density in forest edges could lead to incorrect estimations of carbon stocks. Ten 1ha plots were installed in core and recent (5 to 10 years) forest¿s edges. Trees were measured and wood samples were collected for density analysis. We focused on 2 main research questions: 1) Does the edge effect induce the emergence of species with a higher or lower wood density in edge area? 2) Does wood density differ amongst individuals of the same specie in core and in edge area? While the data is still being explored as we write, preliminary results seem to indicate that there is no significant difference between the overall mean wood density in edge and core plots. Density differences amongst species are still under exploration. If this behaviour still holds, this would imply that the fragmentation of a forested landscape has no short-term effect on the overall forest wood density and thus, no effect on biomass estimations. [less ▲]

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See detailInvestigation of eighteenth-century Prussian blue pigments by PDF analysis
Samain, Louise ULg; Martinetto, Pauline; Bordet, Pierre

Poster (2012, October 30)

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely ... [more ▼]

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely used as a pigment in paintings until the 1970's. The early preparative methods were rapidly recognized as a contributory factor in the fading of the pigment [1], a fading already known by the mid-eighteenth century. The eighteenth-century methods are based on the calcination of dried blood to produce a potassium hexacyanoferrate complex, which is the first of two essential reactants for synthesizing Prussian blue. The second reactant is an iron salt. We successfully reproduced two typical eighteenth-century empirical recipes [2]. The resulting pigments were of variable color quality, ranging from intense blue to blue-gray or blue-green, and exhibit broadened or inexistent Bragg peaks. High-energy powder X-ray diffraction experiments were performed at the ID11 beamline at ESRF, Grenoble, France. The pair distribution function (PDF) of the pure Prussian blue pigments was refined with a three-phase model, in order to take into account the vacancy distribution in the unit cell of Prussian blue. In certain ancient Prussian blues, the PDF analysis revealed the presence of nanocrystalline ferrihydrite, Fe10O14(OH)2, and also identified the presence of alumina hydrate, Al10O14(OH)2, with a particle size of ca. 15 Å. Paint layers prepared from these ancient pigments subjected to accelerated ageing showed a tendency to turn green, a tendency that was often reported in eighteenth- and nineteenth-century books. The presence of particles of hydrous iron(III) oxides was also observed in a genuine Prussian blue sample obtained from an eighteenth-century polychrome sculpture. [1] Kirby, J.; Saunders, D. The National Gallery Technical Bulletin 2004, 25, 73. [2] Dossie, R. The Handmaid to the Arts; Nourse, J.: London, 1758; Le Pileur d'Apligny, M. Traité des couleurs matérielles et de la manière de colorer relativement aux différents arts et métiers; Saugrain et Lamy: Paris, 1779. [less ▲]

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