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See detailImproved detection of chromosomal abnormalities in chronic lymphocytic leukemia by conventional cytogenetics using CpG oligonucleotide and interleukin-2 stimulation: A Belgian multicentric study.
Put, Natalie; Konings, Peter; Rack, Katrina et al

in Genes, Chromosomes & Cancer (2009), 48(10), 843-53

We performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic ... [more ▼]

We performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic leukemia (CLL) referred for routine analysis either at the time of diagnosis (n = 172) or during disease evolution (n = 45). Parallel cultures of peripheral blood or bone marrow were set up with the addition of either the conventional B-cell mitogen 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or a combination of CpG oligonucleotide (CpG) and interleukin-2 (IL-2). Cytogenetic analyses were performed on both cultures. Clonal abnormalities were identified in 116 cases (53%). In 78 cases (36%), the aberrant clone was detected in both cultures. Among these, the percentages of aberrant metaphases were similar in both conditions in 17 cases, higher in the CpG/IL-2 culture in 43 cases, and higher in the TPA culture in 18 cases. Clonal aberrations were detected in only one culture, either in CpG/IL-2 or TPA in 33 (15%) and 5 (2%) cases, respectively. Taken together, abnormal karyotypes were observed in 51% with CpG/IL-2 and 38% with TPA (P < 0.0001). Application of FISH (n = 201) allowed the detection of abnormalities not visible by conventional cytogenetic analysis in 80 cases: del(13q) (n = 71), del(11q) (n = 5), +12 (n = 2), del(14q) (n = 1), and del(17p) (n = 1). In conclusion, our results confirm that CpG/IL-2 stimulation increases the detection rate of chromosomal abnormalities in CLL compared with TPA and that further improvement can be obtained by FISH. However, neither conventional cytogenetics nor FISH detected all aberrations, demonstrating the complementary nature of these techniques. [less ▲]

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See detailImproved District Heating Network Operation by the Integration of High Temperature Heat Pumps
Sartor, Kevin ULg; Lemort, Vincent ULg; Dewallef, Pierre ULg

in Digital proceedings of the 8th Conference on Sustainable Development of Energy, Water and Environment Systems - SDEWES (2013, September 23)

Biomass Combined Heat and Power (CHP) plants connected to district heating networks are a very good opportunity to increase the share of renewable sources into energy systems. Frequently, important ... [more ▼]

Biomass Combined Heat and Power (CHP) plants connected to district heating networks are a very good opportunity to increase the share of renewable sources into energy systems. Frequently, important consumers are connected to ensure a stable base demand of heat throughout the year but they often have higher requirements in terms of temperature (i.e., steam) which involves high level of temperatures in the district heating network during the whole year and high levels of heat losses in the network. This contribution presents the possibility to decrease the level of temperature at which the district heating network operates and to use high temperature heat pumps connected locally at the consumption point to produce steam when needed. An investigation of the global design and integration through thermodynamic simulation models is realized for the University of Liège district heating network. This study is intended to determine the heat pump coefficient of performance and, therefore, to assess the balance between the savings in terms of heat losses and the additional heat pump electricity consumption. [less ▲]

Detailed reference viewed: 58 (12 ULg)
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See detailImproved Domain Decomposition Method for the Helmholtz Equation
Thierry, Bertrand ULg; Antoine, X.; Boubendir, Y. et al

in Proceedings of the 21th International Conference on Domain Decomposition Methods (DD21) (2012, June)

Detailed reference viewed: 43 (4 ULg)
See detailImproved Domain Decomposition Method for the Helmholtz Equation"
Geuzaine, Christophe ULg; Antoine, X.; Boubendir, Y. et al

Scientific conference (2012, January 23)

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See detailImproved Domain Decomposition Method for the Wave Equation in Harmonic Regime
Geuzaine, Christophe ULg; Antoine, X.; Boubendir, Y. et al

in Proceedings of the 6th Advanced Computational Electromagnetics workshop (ACE 2012) (2012, March)

Detailed reference viewed: 30 (2 ULg)
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See detailAn improved ELISA technique for the diagnosis of Psoroptes ovis infestations in cattle
Lonneux, J. F.; Mignon, Bernard ULg; Bossaert, K. et al

Poster (2005)

Detailed reference viewed: 9 (0 ULg)
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See detailImproved emulsifying and foaming of whey proteins after enzymic fat hydrolysis
Blecker, Christophe ULg; Paquot, Michel ULg; Lamberti, I. et al

in Journal of Food Science (1997), 62(1), 48-

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This ... [more ▼]

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This enzymatic lipolysis would convert almost all triglycerides into mono- and diglycerides which have strong surface-active properties. Therefore, the emulsifying and foaming capacities of such modified whey products should be increased in comparison with native products, as shown by results presented at both neutral pH and those similar to a salad dressing emulsion. It was also demonstrated that emulsions and foams formed with lipase-treated whey preparations were more shelf-stable. [less ▲]

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See detailImproved energy resolution of a cyclotron beam for RBS measurements
Chene, Grégoire ULg; Garnir, Henri-Pierre ULg; Marchal, André ULg et al

in Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (2008), 266(10), 2110-2112

Detailed reference viewed: 53 (21 ULg)
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See detailImproved Experimental and Theoretical Energy Levels of Neon I
Chang, E. S.; Schoenfeld, W. G.; Biémont, Emile ULg et al

in Physica Scripta (1994), 49

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See detailImproved extraction of valence transversity distributions from inclusive dihadron production
Radici, Marco; Courtoy, Aurore ULg; Bacchetta, Alessandro et al

in JHEP (2015), 1505

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See detailImproved Finite Element Solvers for the Helmholtz Equation at High Frequencies
Geuzaine, Christophe ULg

Scientific conference (2012, March 29)

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See detailImproved first order moment method for determination of mass-loss rates
Hutsemekers, Damien ULg; Surdej, Jean ULg

in Bianchi, L.; Gilmozzi, R. (Eds.) Mass outflows from stars and galactic nuclei; Proceedings of the Second Torino Workshop (1988)

The first order moment of P Cygni line profiles has been computed for the case of doublets and subordinate line transitions. This improved method can be used for deriving more accurate mass-loss rates of ... [more ▼]

The first order moment of P Cygni line profiles has been computed for the case of doublets and subordinate line transitions. This improved method can be used for deriving more accurate mass-loss rates of planetary nebula nuclei, broad absorption line quasars, etc. [less ▲]

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See detailImproved Forwarding Architecture and Resource Management for Multi-Core Software Routers
Egi, Norbert; Greenhalgh, Adam; Handley, Mark et al

in NPC 2009 Proceedings (2009, October)

Recent technological advances in commodity server architectures, with multiple multi-core CPUs, integrated memory controllers, high-speed interconnects and enhanced network interface cards, provide ... [more ▼]

Recent technological advances in commodity server architectures, with multiple multi-core CPUs, integrated memory controllers, high-speed interconnects and enhanced network interface cards, provide substantial computational capacity and thus an attractive platform for packet forwarding. However, to exploit this available capacity, we need a suitable software platform that allows effective parallel packet processing and resource management. In this paper, we at first introduce an improved forwarding architecture for software routers that enhances parallelism by exploiting hardware classification and multi-queue support, already available in recent commodity network interface cards. After evaluating the original scheduling algorithm of the widely-used Click modular router, we propose solutions for extending this scheduler for improved fairness, throughput and more precise resource management. To illustrate the potential benefits of our proposal, we implement and evaluate a few key elements of our overall design. [less ▲]

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See detailImproved Four-Color Flow Cytometry Method Using Fluo-3 and Triple Immunofluorescence for Analysis of Intracellular Calcium Ion ([Ca2+]I) Fluxes among Mouse Lymph Node B- and T-Lymphocyte Subsets
Greimers, Roland ULg; Trebak, M.; Moutschen, Michel ULg et al

in Cytometry (1996), 23(3), 205-17

A visible-light, dual-laser, flow cytometric method was developed for the simultaneous analysis of intracellular ionized calcium concentration ([Ca2+]i) and three cell-surface markers (CD4, CD8, and Thy-1 ... [more ▼]

A visible-light, dual-laser, flow cytometric method was developed for the simultaneous analysis of intracellular ionized calcium concentration ([Ca2+]i) and three cell-surface markers (CD4, CD8, and Thy-1.2 antigens) by using the calcium probe fluo-3 and using R-phycoerythrin (PE), peridinin chlorophyll-alpha protein (PerCP), and allophycocyanin (APC) conjugated monoclonal antibodies (MoAbs). This improved method was used in the analysis of [Ca2+]i mobilization upon in vitro stimulation with mitogenic lectins [phytohaemagglutinin (PHA) or concanavalin A (ConA)], anti-CD3 MoAbs, or A23187 calcium ionophore in the heterogeneous lymph node cell populations from healthy C57BL/Ka mice. The present results show that the calcium responses were heterogeneous and dependent on the cellular immunophenotype, not only on lectins or anti-CD3 MoAbs stimulation, but also on the receptor-independent A23187 ionophore stimulation. An in situ fluo-3 calibration method (using A23187 and metabolic poisons in Ca2+ /EGTA buffers with known free calcium concentrations) indicated a resting [Ca2+]i in lymphocytes of 103 +/- 23 nM (mean +/- S.D.) but with significant differences between the [Ca2+]i in B cells and in all of the T-cell subsets (CD4+Thy-1+, CD4+Thy-1-, and CD8+T cells). Both the B cells and the T-cell subsets showed an increase of fluo-3 fluorescence upon in vitro stimulation with ConA or PHA, but the calcium mobilization following lectin stimulation was time delayed in all T-cell subsets. Only the T cells, including the CD4+Thy-1- subset, responded to anti-CD3 MoAbs. The percentage of responding cells upon stimulation with ConA was higher in T cells than in B cells. By contrast, PHA gave a higher response in B cells. After stimulation with different mitogens, [Ca2+]i increased in both CD4+ and CD8+ T-cell subsets. However, the percentage of responding cells was far higher in the CD4+Thy-1+ subset than in the CD4+Thy-1- or the CD8+T-cell subsets. The stimulation with A23187 ionophore induced a higher calcium response in B cells than in T cells. Interestingly, it also induced greater Ca2+ mobilization in CD4+ than in CD8+T cells. These results demonstrate the potential use of fluo-3 simultaneously with three fluorescein (FITC)-compatible fluorochromes. This technique may be useful for investigating the role of the CD4+Thy-1-T cells, a rare subset that is abnormally expanded in a murine acquired immunodeficiency syndrome (murine AIDS). [less ▲]

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See detailImproved head and neck FDG-PET imaging using segmented attenuation correction.
PAULUS, P.; HENRY, S.; HUSTINX, Roland ULg et al

in European Journal of Nuclear Medicine (1997), 24

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See detailAn improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE).
Rafamantanana, M. H.; Rozet, Eric ULg; Raoelison, G. E. et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2009), 877(23), 2396-402

The simultaneous quantification of madecassoside, asiaticoside, madecassic acid and asiatic acid in Centella asiatica by HPLC-UV is proposed. Asiaticoside was used as reference for the quantification of ... [more ▼]

The simultaneous quantification of madecassoside, asiaticoside, madecassic acid and asiatic acid in Centella asiatica by HPLC-UV is proposed. Asiaticoside was used as reference for the quantification of heterosides and asiatic acid for aglycones. The evaluation of the extraction efficiency of the four molecules led to use Soxhlet extraction for 8 h. The method was validated and was found to be accurate in the concentration range of 1.0-3.0 mg/ml for asiaticoside and 0.5-2.0 mg/ml for asiatic acid with CV <3% for all investigated compounds. LOD and LOQ were, respectively, 0.0113 and 1.0 mg/ml for asiaticoside and 0.0023 and 0.5 mg/ml for asiatic acid. This method was shown to be convenient for routine analysis of samples of C. asiatica. [less ▲]

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See detailImproved IL-2 detection for determination of helper T lymphocyte precursor frequency in limiting dilution assay
Winandy, Marie ULg; Lewalle, Philippe; Deneys, Véronique et al

in Journal of Immunological Methods (1998), 215

In the context of allogeneic bone marrow transplantation, an accurate estimate of the risk of developing graft-versus-host disease GVHD. is of major interest. The pre-transplant frequency of donor’s ... [more ▼]

In the context of allogeneic bone marrow transplantation, an accurate estimate of the risk of developing graft-versus-host disease GVHD. is of major interest. The pre-transplant frequency of donor’s helper T-lymphocyte precursors HTLp. directed against host’s antigens may be helpful in predicting this risk. This technique relies on an indirect measurement of interleukin-2 IL-2. secreted by the HTLp, as assessed by the proliferation of an IL-2 dependent cell line. Many authors use the murine CTLL-2 cell line in this assay, but these cells do not respond to the presence of minute amounts of IL-2 in the culture medium, and thus do not discriminate between the absence or the presence of very low levels of IL-2. We therefore decided to compare CTLL-2 with another IL-2 dependent cell line, the murine A9.12 cell line. A comparison was made using serial dilutions of recombinant human IL-2, limiting dilutions of baby hamster kidney BHK. cells transfected with human IL-2 gene and in the context of clinical tests performed for the detection of pre-transplant HTLp. Both the sensitivity and reliability of the tests were better using A9.12. We conclude that the A9.12 cell line might be a more suitable tool for pre-transplant HTLp determinations before allogeneic bone marrow transplantation or whenever low IL-2 levels are to be measured. [less ▲]

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See detailImproved implementation and robustness study of the X-FEM for stress analysis around cracks
Béchet, Eric ULg; Minnebol, H.; Moes, N. et al

in International Journal for Numerical Methods in Engineering (2005), 64(8), 1033-1056

Numerical crack propagation schemes were augmented in an elegant manner by the X-FEM method. The use of special tip enrichment functions, as well as a discontinuous function along the sides of the crack ... [more ▼]

Numerical crack propagation schemes were augmented in an elegant manner by the X-FEM method. The use of special tip enrichment functions, as well as a discontinuous function along the sides of the crack allows one to do a complete crack analysis virtually without modifying the underlying mesh, which is of industrial interest, especially when a numerical model for crack propagation is desired. This paper improves the implementation of the X-FEM method for stress analysis around cracks in three ways. First, the enrichment strategy is revisited. The conventional approach uses a 'topological' enrichment (only the elements touching the front are enriched). We suggest a 'geometrical' enrichment in which a given domain size is enriched. The improvements obtained with this enrichment are discussed. Second, the conditioning of the X-FEM both for topological and geometrical enrichments is studied. A preconditioner is introduced so that 'off the shelf' iterative solver packages can be used and perform as well on X-FEM matrices as on standard FEM matrices. The preconditioner uses a local (nodal) Cholesky based decomposition. Third, the numerical integration scheme to build the X-FEM stiffness matrix is dramatically improved for tip enrichment functions by the use of an ad hoc integration scheme. A 2D benchmark problem is designed to show the improvements and the robustness. Copyright (c) 2005 John Wiley & Sons, Ltd. [less ▲]

Detailed reference viewed: 60 (6 ULg)