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See detailGenetic control of isozymes in the primary gene pool Phaseolus lunatus L.
Bi, Irié Zoro; Maquet, Alain; Wathelet, Bernard ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999)

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See detailGenetic correlations among body condition score, yield and fertility in multiparous cows using random regression models
Bastin, Catherine ULg; Gillon, Alain ULg; Massart, Xavier et al

in Proceedings of the 9th World Congress on Genetics Applied to Livestock Production (2010, August)

Genetic correlations between body condition score (BCS) in lactation 1 to 3 and four economically important traits (days open, 305-days milk, fat, and protein yields recorded in the first 3 lactations ... [more ▼]

Genetic correlations between body condition score (BCS) in lactation 1 to 3 and four economically important traits (days open, 305-days milk, fat, and protein yields recorded in the first 3 lactations) were estimated on about 12,500 Walloon Holstein cows using 4-trait random regression models. Results indicated moderate favorable genetic correlations between BCS and days open (from -0.46 to -0.62) and suggested the use of BCS for indirect selection on fertility. However, unfavorable genetic correlations between BCS and yields (from -0.16 to -0.71) indicated that selection on BCS would have deleterious effects on milk, fat, and protein yields, especially in lactation greater than 1. [less ▲]

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See detailGenetic correlations between methane production and milk fatty acid contents of Walloon Holstein cattle throughout the lactation
Vanrobays, Marie-Laure ULg; Vandenplas, Jérémie ULg; Bastin, Catherine ULg et al

Poster (2015, April 16)

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake ... [more ▼]

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake. Therefore, there is a growing interest in mitigating these emissions. Acetate and butyrate have common bio-chemical pathways with CH4. Because some milk fatty acids (FA) arise from acetate and butyrate, milk FA are often considered as potential predictors of CH4. However, relationships between these traits remain unclear. Moreover, the evolution of the phenotypic and genetic correlations of CH4 and milk FA across days in milk (DIM) has not been evaluated. The main goal of this study was to estimate genetic correlations between CH4 and milk FA contents throughout the lactation. Calibration equations predicting daily CH4 production (g/d) and milk FA contents (g/100 dL of milk) from milk mid-infrared (MIR) spectra were applied on MIR spectra related to Walloon milk recording. Data included 243,260 test-day records (between 5 and 365 DIM) from 33,850 first-parity Holstein cows collected in 630 herds. Pedigree included 109,975 animals. Bivariate (i.e., CH4 production and one of the FA traits) random regression test-day models were used to estimate genetic parameters of CH4 production and 7 groups of FA contents in milk. Saturated (SFA), short-chain (SCFA), and medium-chain FA (MCFA) showed positive averaged daily genetic correlations with CH4 production (from 0.25 to 0.29). Throughout the lactation, genetic correlations between SCFA and CH4 were low in the beginning of the lactation (0.11 at 5 DIM) and higher at the end of the lactation (0.54 at 365 DIM). Regarding SFA and MCFA, genetic correlations between these groups of FA and CH4 were more stable during the lactation with a slight increase (from 0.23 to 0.31 for SFA and from 0.23 to 0.29 for MCFA, at 5 and 365 DIM respectively). Furthermore, averaged daily genetic correlations between CH4 production and monounsaturated (MUFA), polyunsaturated (PUFA), unsaturated (UFA), and long-chain FA (LCFA) were low (from 0.00 to 0.15). However, these genetic correlations varied across DIM. Genetic correlations between CH4 and MUFA, PUFA, UFA, and LCFA were negative in early lactation (from -0.24 to -0.34 at 5 DIM) and increased afterward to become positive from 15 weeks till the end of the lactation (from 0.14 to 0.25 at 365 DIM). Finally, these results indicate that genetic and, therefore, phenotypic correlations between CH4 production and milk FA vary following lactation stage of the cow, a fact still often ignored when trying to predict CH4 production from FA composition. [less ▲]

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See detailGenetic correlations of days open with production traits and contents in milk of major fatty acids predicted by mid-infrared spectrometry.
Bastin, Catherine ULg; Berry, D. P.; Soyeurt, Hélène ULg et al

in Journal of Dairy Science (2012), 95(10), 6113-21

The objective of this study was to estimate the genetic relationships between days open (DO) and both milk production traits and fatty acid (FA) content in milk predicted by mid-infrared spectrometry. The ... [more ▼]

The objective of this study was to estimate the genetic relationships between days open (DO) and both milk production traits and fatty acid (FA) content in milk predicted by mid-infrared spectrometry. The edited data set included 143,332 FA and production test-day records and 29,792 DO records from 29,792 cows in 1,170 herds. (Co)variances were estimated using a series of 2-trait models that included a random regression for milk production and FA traits. In contrast to the genetic correlations with fat content, those between DO and FA content in milk changed considerably over the lactation. The genetic correlations with DO for unsaturated FA, monounsaturated FA, long-chain FA, C18:0, and C18:1 cis-9 were positive in early lactation but negative after 100 d in milk. For the other FA, genetic correlations with DO were negative across the whole lactation. At 5 d in milk, the genetic correlation between DO and C18:1 cis-9 was 0.39, whereas the genetic correlations between DO and C6:0 to C16:0 FA ranged from -0.37 to -0.23. These results substantiated the known relationship between fertility and energy balance status, explained by the release of long-chain FA in early lactation, from the mobilization of body fat reserves, and the consequent inhibition of de novo FA synthesis in the mammary gland. At 200 d in milk, the genetic correlations between DO and FA content ranged from -0.38 for C18:1 cis-9 to -0.03 for C6:0. This research indicates an opportunity to use FA content in milk as an indicator trait to supplement the prediction of genetic merit for fertility. [less ▲]

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See detailGenetic depletion of the dual specificity protein phosphatase DUSP3 promotes LLC Lung tumour metastasis
Vandereyken, Maud ULg; Amand, Mathieu; Van Overmeire, Eva et al

Poster (2015, June)

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell ... [more ▼]

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell cycle regulation and is up-regulated in several human cancers. The physiological role of this phosphatase is, however, poorly understood. We have recently generated a DUSP3 knockout mouse by homologous recombination. The obtained mice have no spontaneous phenotype or pathology. However, DUSP3 deficiency prevented neo-vascularization of subcutaneously transplanted Matrigel plugs and Lung Lewis Carcinoma (LLC) tumours, suggesting an involvement of DUSP3 in tumour angiogenesis. Considering the importance of angiogenesis in metastatic formation, our study aimed to investigate the role of DUSP3 in metastatic dissemination. To do so, we used the LLC experimental metastasis model that shortcuts the intravasation/extravasation processes by injecting intravenously the LLC and the B16 (metastatic melanoma cell line) cells. Surprisingly, LLC, but not B16, lung metastasis developed twice faster in DUSP3-KO than WT mice. The enhanced LLC metastatic growth in DUSP3-/- mice was transferable to WT mice via DUSP3-/- bone marrow adoptive transfer, suggesting an involvement of the hematopoietic compartment in the observed phenotype. This was confirmed by a higher infiltration of neutrophils and macrophages in the lungs of DUSP3-KO compared to WT mice after LLC injection. This infiltration was correlated with higher expression of the chemokine receptor CCR2 in LLC-bearing DUSP3-KO lungs macrophages. Interestingly, LLC, but not B16 cells, were found to secrete high level of CCL2/MCP1, the CCR ligand chemokine. In line with this observation, we found that DUSP3-/- bone marrow derived-macrophages have a higher migration potential in response to LLC, but not B16, -conditionned medium. Altogether, our results suggest that DUSP3 plays an important role in metastatic dissemination/growth by a mechanism involving the control of CCR2-CCL2 chemoattraction axis in macrophages. [less ▲]

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See detailGenetic depletion of the dual specificity protein phosphatase DUSP3 promotes LLC Lung tumour metastasis
Vandereyken, Maud ULg; Amand, Mathieu ULg; Van Overmeire, Eva et al

Conference (2015, June)

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell ... [more ▼]

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell cycle regulation and is up-regulated in several human cancers. The physiological role of this phosphatase is, however, poorly understood. We have recently generated a DUSP3 knockout mouse by homologous recombination. The obtained mice have no spontaneous phenotype or pathology. However, DUSP3 deficiency prevented neo-vascularization of subcutaneously transplanted Matrigel plugs and Lung Lewis Carcinoma (LLC) tumours, suggesting an involvement of DUSP3 in tumour angiogenesis. Considering the importance of angiogenesis in metastatic formation, our study aimed to investigate the role of DUSP3 in metastatic dissemination. To do so, we used the LLC experimental metastasis model that shortcuts the intravasation/extravasation processes by injecting intravenously the LLC and the B16 (metastatic melanoma cell line) cells. Surprisingly, LLC, but not B16, lung metastasis developed twice faster in DUSP3-KO than WT mice. The enhanced LLC metastatic growth in DUSP3-/- mice was transferable to WT mice via DUSP3-/- bone marrow adoptive transfer, suggesting an involvement of the hematopoietic compartment in the observed phenotype. This was confirmed by a higher infiltration of neutrophils and macrophages in the lungs of DUSP3-KO compared to WT mice after LLC injection. This infiltration was correlated with higher expression of the chemokine receptor CCR2 in LLC-bearing DUSP3-KO lungs macrophages. Interestingly, LLC, but not B16 cells, were found to secrete high level of CCL2/MCP1, the CCR ligand chemokine. In line with this observation, we found that DUSP3-/- bone marrow derived-macrophages have a higher migration potential in response to LLC, but not B16, -conditionned medium. Altogether, our results suggest that DUSP3 plays an important role in metastatic dissemination/growth by a mechanism involving the control of CCR2-CCL2 chemoattraction axis in macrophages. [less ▲]

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See detailGenetic Determinants Of Bovine Leukemia Virus Pathogenesis
Willems, Luc ULg; Burny, A.; Collete, Delphine et al

in Aids Research and Human Retroviruses (2000), 16(16), 1787-95

The understanding of HTLV-induced disease is hampered by the lack of a suitable animal model allowing the study of both viral replication and leukemogenesis in vivo. Although valuable information has been ... [more ▼]

The understanding of HTLV-induced disease is hampered by the lack of a suitable animal model allowing the study of both viral replication and leukemogenesis in vivo. Although valuable information has been obtained in different species, such as rabbits, mice, rats, and monkeys, none of these systems was able to conciliate topics as different as viral infectivity, propagation within the host, and generation of leukemic cells. An alternate strategy is based on the understanding of diseases induced by viruses closely related to HTLV-1, like bovine leukemia virus (BLV). Both viruses indeed belong to the same subfamily of retroviruses, harbor a similar genomic organization, and infect and transform cells of the hematopoietic system. The main advantage of the BLV system is that it allows direct experimentation in two different species, cattle and sheep. [less ▲]

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See detailGenetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431
Aguilar Galvez, Ana Consuelo; Dubois Dauphin, Robin ULg; Campos Gutierrez, David Carlos et al

in Food Science and Biotechnology (2011), 20(2), 289-296

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanalproduced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and ... [more ▼]

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanalproduced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kb HindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii. [less ▲]

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See detailGenetic diagnosis and emergence of patients and relatives' evidence-based activism
Duysens, Fanny ULg

Conference (2016, December 07)

Health- and disease-related events may be of highly significance for patients and their relatives. Naming a medical diagnosis is one of them. It carries the inherent power of (re)configuring the ... [more ▼]

Health- and disease-related events may be of highly significance for patients and their relatives. Naming a medical diagnosis is one of them. It carries the inherent power of (re)configuring the individuals' life courses, which are moreover closely linked to those of their relatives, and especially blood families in the case of (hereditary) genetic diseases. Drawing on ethnographic observations and narratives from patients and relatives involved in some Belgian organizations concerned with genetic disorders, this paper is interested in the ways these concerned people make sense of the naming of a medical diagnosis event by (re)interpreting their past familial stories, trying to intervene in the present, and designing a desired future. Then, it explores the case of the annual charity TV show "Téléthon", which is an initiative launched at the initiative of some French-speaking patients’ organizations (POs) concerned with neuromuscular disorders in the aims at putting awareness on rare disorders and raising funds in order to support biomedical research, especially in the field of medical genetics. The point is to question the dynamic relationships between the individual and familial experiences and such collective mobilizations, that is to say the dynamic relationships between individual and collective dimensions of POs' evidence-based activism. The argument is that such forms of activism participate in the co-construction of "gene worlds" (Timmermans & Shostak, 2015), through the relations between various actors and institutions: POs, scientific and medical professionals, public health institutions, and healthcare systems. [less ▲]

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See detailGenetic diagnosis of Duchenne and Becker muscular dystrophy using multiplex ligation-dependent probe amplification in Rwandan patients.
Uwineza, Annette ULg; Hitayezu, Janvier; Murorunkwere, Seraphine et al

in Journal of tropical pediatrics (2013)

Duchenne and Becker muscular dystrophies are the most common clinical forms of muscular dystrophies. They are genetically X-linked diseases caused by a mutation in the dystrophin (DMD) gene. A genetic ... [more ▼]

Duchenne and Becker muscular dystrophies are the most common clinical forms of muscular dystrophies. They are genetically X-linked diseases caused by a mutation in the dystrophin (DMD) gene. A genetic diagnosis was carried out in six Rwandan patients presenting a phenotype of Duchenne and Becker muscular dystrophies and six asymptomatic female carrier relatives using multiplex ligation-dependent probe amplification (MLPA). Our results revealed deletion of the exons 48-51 in one patient, an inherited deletion of the exons 8-21 in two brothers and a de novo deletion of the exons 46-50 in the fourth patient. No copy number variation was found in two patients. Only one female carrier presented exon deletion in the DMD gene. This is the first cohort of genetic analysis in Rwandan patients affected by Duchenne and Becker muscular dystrophies. This report confirmed that MLPA assay can be easily implemented in low-income countries. [less ▲]

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See detailGenetic Differentiation Between Native and Invasive Populations of Quercus rubra L.
Merceron, Nastasia ULg

Conference (2014)

We investigated the role of interaction between environmental conditions and genetics in the success of tree invasion. Indeed, invasiveness is expressed only under certain environments: for example ... [more ▼]

We investigated the role of interaction between environmental conditions and genetics in the success of tree invasion. Indeed, invasiveness is expressed only under certain environments: for example, European populations of Acer negundo L. present higher growth and a longer growing season length explained by earlier budburst compared to that of U.S. populations of A. negundo. This may be related to rapid changes in introduced populations. Provenance tests are appropriate tools for studying genetic differentiation by comparing populations of different origins under several environmental conditions. Northern Red Oak (Quercus rubra) which was first introduced from USA to France as an ornamental species has received a strong interest in forestry since the late 19th century. This interest prompted the installation of comparative trials to establish a breeding program during the 80s-90s at the demand of forest managers. Recently, European forest managers seek to evaluate its invasiveness and to limit its natural expansion that impedes the regeneration of sessile or pedunculate oaks. Two provenance trials have been settled in South-West and North-East of France, containing 66 American and 60 European provenances, corresponding to 40000 trees per trial, which were followed between 1982 and 2012 on traits related to fitness. Within each trial, differences between ranges would reflect the existence of genetic differentiation between populations, while differences between trials would indicate the existence of phenotypic plasticity. Statistical analyses showed the existence of genetic differentiation, invasive European populations demonstrating superior growth compared to native populations. Regarding phenology, the results are inconclusive, probably due to the strong inter-annual variability of these traits and the availability of a single measurement. A monitoring of leaf and fruiting phenology, traits heavily involved in the determination of fitness, are thus required. On the other hand, in order to evaluate the adaptive nature of these differentiations, analyses of diversity molecular markers will also be undertaken. [less ▲]

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See detailGenetic differentiation of native and introduced populations of Quercus rubra L
Merceron, Nastasia ULg

Poster (2016)

Native to North America, Northern red oak (Quercus rubra) was introduced in Europe in the XVIIth century for ornamental and forestry purposes. This species is now widespread in European forests due to ... [more ▼]

Native to North America, Northern red oak (Quercus rubra) was introduced in Europe in the XVIIth century for ornamental and forestry purposes. This species is now widespread in European forests due to plantations and natural regeneration. In invasive herbaceous plants, introduced populations are often genetically different from native populations. However, this has been poorly investigated in exotic tree species. Our objective was to explore the phenotypic variation between native and introduced populations of Q. rubra and to test for adaptation to the new environmental conditions since the introduction. We used three progeny test gardens, in South-Western, Central and North-Eastern France, composed of 106 American and European populations with 1-25 families per population. The gardens were settled from 1980s and trees were monitored regularly for growth (diameter, height) and leaf phenology (budburst, coloration). Since trees had reached sexual maturity, we have monitored acorn production for two years. Within each garden, data were analyzed using mixed analyses of variance; Qst indexes were calculated to evaluate genetic differentiation between populations. Overall, introduced populations presented higher trait values than native populations: growth rate was higher and spring phenology was advanced. Fruit set was higher in introduced trees, although depending of the year. Qst estimates clearly demonstrated the existence of a high genetic differentiation between native populations, for growth and phenology. Introduced populations presented a lower level of differentiation than native populations. These results suggest several hypotheses: (i) introduced populations only represent a part of the global diversity existing in the native range (ii) populations have evolved since introduction under new environmental selective pressures (ii) populations were selected by man since introduction. These hypotheses are being investigated, notably through a molecular approach. [less ▲]

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See detailGenetic disorders and cerebellar structural abnormalities in childhood
RAMAEKERS, Vincent ULg; Heimann, G.; Reul, J. et al

in Brain : a journal of neurology (1997), 120 ( Pt 10)

Amongst 78 patients with either unilateral or bilateral (ponto-) cerebellar hypoplasia, atrophy or lesions on neuro-imaging (CT and/or MRI), 16 showed unilateral hypoplasia or lesions, 15 vermis defects ... [more ▼]

Amongst 78 patients with either unilateral or bilateral (ponto-) cerebellar hypoplasia, atrophy or lesions on neuro-imaging (CT and/or MRI), 16 showed unilateral hypoplasia or lesions, 15 vermis defects, nine pontocerebellar hypoplasia, 10 non-progressive conditions with bilateral cerebellar hemisphere hypoplasia or lesions and 28 progressive cerebellar atrophy. Known genetic conditions did not occur with unilateral cerebellar involvement, whereas a high incidence of mostly autosomal recessively inherited diseases could be diagnosed in more than half of the patients with either pontocerebellar hypoplasia or progressive bilateral cerebellar atrophy. A minority of patients with vermis defects or non-progressive cerebellar hypoplasia suffered from genetic conditions. An overview of the literature is presented describing genetic and non-genetic syndromes, or metabolic disorders associated with cerebellar structural abnormalities. From these data, new proposals for improved diagnostic investigations will be presented. [less ▲]

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See detailGenetic diversity among some wild populations of lima bean (Phaseolus lunatus L.) proceeding from Central Valley of Costa Rica
Maquet, Alain; Wathelet, Bernard ULg; Dubois, Sophie et al

in Annual Report of the Bean Improvment Cooperative (1994)

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See detailGenetic diversity analysis of wild Arracacia species according to morphological and molecular markers
Blas, R.; Ghislain, M.; del Rosario Herrera, M. et al

in Genetic Resources and Crop Evolution (2008), 55(5), 625-642

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See detailGenetic diversity and amplification of different clostridial [FeFe] hydrogenases by group-specific degenerate primers
Calusinska, Magdalena ULg; Joris, Bernard ULg; Wilmotte, Annick ULg

in Letters in Applied Microbiology (2011), 53

Aims: The aim of this study was to explore and characterize the genetic diversity of [FeFe] hydrogenases in a representative set of strains from Clostridium sp. and to reveal the existence of neither yet ... [more ▼]

Aims: The aim of this study was to explore and characterize the genetic diversity of [FeFe] hydrogenases in a representative set of strains from Clostridium sp. and to reveal the existence of neither yet detected nor characterized [FeFe] hydrogenases in hydrogen-producing strains. Methods and Results: The genomes of 57 Clostridium strains (34 different genotypic species), representing six phylogenetic clusters based on their 16S rRNA sequence analysis (cluster I, III, XIa, XIb, XIV and XVIII), were screened for different [FeFe] hydrogenases. Based on the obtained alignments, ten pairs of [FeFe] hydrogenase cluster-specific degenerate primers were newly designed. Ten Clostridium strains were screened by PCRs to assess the specificity of the primers designed and to examine the genetic diversity of [FeFe] hydrogenases. Using this approach, a diversity of hydrogenase genes was discovered in several species previously shown to produce hydrogen in bioreactors: Clostridium sartagoforme, Clostridium felsineum, Clostridium roseum and Clostridium pasteurianum. Conclusions: The newly designed [FeFe] hydrogenase cluster-specific primers, targeting the cluster-conserved regions, allow for a direct amplification of a specific hydrogenase gene from the species of interest. Significance and Impact of the Study: Using this strategy for a screening of different Clostridium ssp. will provide new insights into the diversity of hydrogenase genes and should be a first step to study a complex hydrogen metabolism of this genus. [less ▲]

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