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Peer Reviewed
See detailIsolation of novel hydrolytic genes from an Antarctic metagenomic library
Pipers, D.; Berlemont, R.; Power, P. et al

Poster (2008)

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See detailIsolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated Nucleoli.
Thiry, Marc ULg; Ploton, Dominique

in Methods in Molecular Biology (Clifton, N.J.) (2008), 463

Here we describe a new, rapid method for isolating nucleoli from Ehrlich tumor cells that preserves their morphological integrity and high transcriptional activity. Until now, methods for isolation of ... [more ▼]

Here we describe a new, rapid method for isolating nucleoli from Ehrlich tumor cells that preserves their morphological integrity and high transcriptional activity. Until now, methods for isolation of nucleoli were generally assumed to empty one of their three main compartments, the fibrillar center, of its contents. This new method consists of sonicating cells in an isotonic medium containing MgSO(4), spermidine, and spermine, followed by separation of nucleoli through a Percoll density gradient. Using the nonisotopic approach of labelling with BrUTP, we have further investigated the dynamics of nascent ribosomal RNAs (rRNAs) within morphologically intact isolated nucleoli at the electron microscope level. We show that ribosomal transcripts are elongated in the cortex of the fibrillar center and then enter the surrounding dense fibrillar component. [less ▲]

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See detailIsolation of nucleoli from ELT cells: a quick new method that preserves morphological integrity and high transcriptional activity.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1996), 228(1), 125-31

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to ... [more ▼]

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to empty one of the three major compartments of the nucleolus, the fibrillar center, of its content. We have used the AgNOR staining and in vitro transcription assay to test the degree of structural and functional preservation of the isolated nucleoli. Our results demonstrate the value of our procedure as a reliable tool for biochemical and ultrastructural studies on the nucleolus. Moreover, these proprieties prompt us to investigate the rRNA synthesis, using a nonisotopic approach, within morphologically intact isolated nucleoli. Thus, we show that newly synthesized rRNA transcripts are located not only in the dense fibrillar component, but also indubitably in the fibrillar center. [less ▲]

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See detailIsolation of O157 : H7 and other enterohaemorrhagic Escherichia coli from foodstuffs.
Daube, Georges ULg; Chahed, Amina

in Factors affecting the microbial quality of meat, 4. Microbial methods for the meat industry. Concerted Action CT94-1456 (1997)

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See detailIsolation of PAG from buffalo (Bubalus bubalis) placenta
Barbato, O.; Melo de Sousa, Noelita ULg; Klisch, K. et al

in Reproduction (Cambridge, England). Abstract Series (2006), Suppl

Detailed reference viewed: 8 (1 ULg)
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See detailIsolation of plunc (palate, lung and nasal epithelium clone protein) in the bronchoalveolar lavage from dogs: preliminary results
Clercx, A.; Vandenbussche, G.; Ruysschaert, J. M. et al

in 13th ESVIM Meeting - Uppsala - Septembre 2003 (2003, September)

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See detailIsolation of pregnancy-associated glycoproteins of the American bison (Bison bison) at first half of pregnancy
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in General and Comparative Endocrinology (2008), 155(1), 164-175

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins ... [more ▼]

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins were purified from foetal cotyledonary tissues (CT) and liquid cotyledonary-carrying proteins (LCP) leaking from damaged cells. Our protocols successfully indicated the usefulness of AmbPAG protein identification, especially from LCP fraction. The AmbPAGs were extracted, precipitated and eluted during DEAE cellulose chromatography. The richest protein fractions were further chromatographed on VVA (Vicia villosa agglutinin affinity column), then characterised by mono- and bi-dimensional electrophoresis, Western blot and N-terminal amino acid (aa) sequence. After being transferred to PVDF membranes, three selected VVA-purified AmbPAG isoforms differing in molecular masses and isoelectric points (Ip 4-4.6) were selected for sequencing. One identified N-terminal 25 aa sequence of AmbPAG72 kDa CT form was identified as completely new (RGSNI_TSLPLQNVIDLFYVGNITIG). Two other AmbPAG proteins purified from different sources (74 kDa CT and 76 kDa LCP forms; RGSNLTIHPLRNIRDIFYVGNITIG) were identical or corresponded to N-terminus of various bovine PAGs (boPAG). The two AmbPAGs (74 kDa CT and 76 kDa LCP) revealed identical micro-sequence to boPAG7; and were similar mainly to bovine PAG4, -6, -15 and -17 precursors that were identified by full-length sequencing derived from cDNA cloning. The novel sequence of the AmbPAG (72 kDa CT) was related to some boPAG and various other ruminant PAG precursors (caprine and ovine). All three identified AmbPAG sequences were also relatively similar to mature forms of purified native boPAG56-75kDa proteins. This is the first report indicating aa sequences of native AmbPAG proteins purified from placenta (CT and LCP) of bison species. The N-terminal sequences of the AmbPAGs have been deposited in the EMBL-EBI database (UniProtKB; Accession Nos.: P84916, P84917 and P84918). (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

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See detailIsolation of shiga toxin-producing Escherichia coli from a South American camelid (Lama guanicoe) with diarrhea.
Mercado, E. C.; Rodriguez, Sabrina ULg; Elizondo, A. M. et al

in Journal of Clinical Microbiology (2004), 42(10), 4809-11

Shiga toxin-producing Escherichia coli belonging to serotype O26:H11 was isolated from a 2-month-old guanaco with severe watery diarrhea. E. coli colonies carried the stx1 and eae genes, showed localized ... [more ▼]

Shiga toxin-producing Escherichia coli belonging to serotype O26:H11 was isolated from a 2-month-old guanaco with severe watery diarrhea. E. coli colonies carried the stx1 and eae genes, showed localized adherence to HEp-2 cells, and produced enterohemolysin. A serological response to lipopolysaccharide O26 was observed at the onset of diarrhea. [less ▲]

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See detailISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
ELLING, Lothar; GULDENBERG, Birgit; GROTHUS, Marita et al

in Biotechnology & Applied Biochemistry (1995), 21(Part 1), 29-37

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the ... [more ▼]

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time [less ▲]

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See detailIsolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase
Nguyen-Distèche, Martine ULg; Leyh-Bouille, Mélina; Ghuysen, Jean-Marie ULg

in Biochemical Journal (1982), 207(1), 109-115

The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the form of an effective, penicillin-sensitive D-alanyl-D-alanine-cleaving peptidase exhibiting high ... [more ▼]

The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the form of an effective, penicillin-sensitive D-alanyl-D-alanine-cleaving peptidase exhibiting high transpeptidase activity (greater than 95%) and very low carboxy-peptidase activity (less than 5%). The penicillin-binding protein/transpeptidase can be extracted directly from the mycelium with N-cetyl-NNN-trimethylammonium bromide (Cetavlon) and subsequently obtained at 90% purity and with an 8000-fold specific enrichment (when compared with the activity of the isolated membranes) by a two-step procedure involving Sephadex filtration and affinity chromatography on ampicillin-linked CH Sepharose 4B in the presence of detergent. At saturating concentrations of the co-substrates diacetyl-L-Lys-D-Ala-D-Ala and Gly-Gly, the catalytic-centre activity is about 0.3 s-1. [less ▲]

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Peer Reviewed
See detailIsolation of the missing 5-end of the encoding region of the bovine leukemia virus cell receptor gene.
Ban, J.; Truong, A. T.; Horion, B. et al

in Archives of Virology (1994), 138(3-4),

Detailed reference viewed: 14 (2 ULg)
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See detailIsolation of the pregnancy-associated glycoprotein 1 (PAG-1) from zebu (Bos indicus) placenta.
Melo de Sousa, Noelita ULg; Remy, B.; El Amiri, B. et al

in Proceedings of the 16th Scientific Meeting of the European Embryo Transfer Association. (2000)

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See detailL'isolation thermique de la toiture inclinée - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg

Book (1999)

In 1996, for primarily environmental motivations, the Walloon Government reinforced the requirements as regards thermo isolation of the buildings of the sectors residential and tertiary. For the new ... [more ▼]

In 1996, for primarily environmental motivations, the Walloon Government reinforced the requirements as regards thermo isolation of the buildings of the sectors residential and tertiary. For the new housing, the level of total thermo isolation necessary K55 replaced the K70 level, while a K65 level was fixed for the lately built offices and the schools. In alternative, the energy needs Be 450 replaced the needs Be 500 required previously for the new housings. The intervention of the originators is essential because it returns to them to integrate the performances to be reached, as of the initial stages of the process of design. These performances should not however slow down their creativity; on the contrary, it is a question of building buildings combining beauty, comfort, durability and economy. Conscious of the importance of information to be brought to the professionals on the one hand and to the public on the other hand, the Walloon region has set up, for a few years, a multiform device of information and formation, relaying the research undertaken by the Scientific and Technical Center of Construction and the Universities. A first handy guide intended for the architects explored the methodology of design of the thermo isolation of the hollow wall. Its authors, Francy Simon, Professor at the Catholic of Leuwen, and Jean-Marie Hauglustaine, ir University. of research - doctorand at the University of Liege, continue this approach for the thermo isolation of the inclined roof, proposing a coherent step, draft with the project. In this guide, the architect will find a step conceptual integrated allowing him to direct and adapt his choices, by the recourse in particular to tables presenting the current systems of inclined roofs, their performances, as well as a predimensioning thicknesses of insulator and type of complementary vapor barrier possibly necessary. Another guide intended for roofers and for carpenters is currently diffused by Funds of Professional training of Construction, while the private individuals can find booklets and councils which go in the same direction, near the Counters of Energy. These achievements are integrated in the policy which the Rational Use of Energy (RUE) in the buildings has carried out the Walloon region for several years in order to support. Belgium indeed engaged, in Rio and Kyoto, to decrease the consumption of energy and thus the CO2 emissions, in order to reduce the greenhouse effect. Let us guarantee that we will reach that point thanks to the efforts of all. [less ▲]

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See detailL'isolation thermique de la toiture plate - Guide pratique pour les architectes
Simon, Francy; Hauglustaine, Jean-Marie ULg; Liesse, Sophie et al

Book (2003)

Detailed reference viewed: 103 (2 ULg)