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See detailImproved antigenic methods for differential diagnosis of bovine, caprine, and cervine alphaherpesviruses related to bovine herpesvirus 1
Keuser, V.; Schynts, F.; Detry, Bruno et al

in Journal of Clinical Microbiology (2004), 42(3), 1228-1235

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four ... [more ▼]

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four other alphaherpesviruses of ruminants-namely, BoHV-5, caprine herpesvirus 1 (CpHV-1), cervine herpesvirus 1 (CvHV-1) and CvHV-2-diagnostic tests able to discriminate BoHV-1 from these related viruses are needed to avoid misdiagnosis, especially because some of these viruses are able to cross the species barrier. In this study, murine monoclonal antibodies (MAbs) specific for BoHV-1, BoHV-5, CpHV-1, CvHV-1, and CvHV-2 were produced with the aim of setting up an immunofluorescence assay able to discriminate between these related herpesviruses. Produced MAbs were selected for their viral specificity by enzyme-linked immunosorbent assay and indirect immunofluorescence staining of virus-infected cells. Radioimmunoprecipitation characterization of the selected MAbs revealed that four of them are directed against glycoprotein C (gC) and one of them is directed against gD of these related viruses. The obtained results demonstrate that the antibodies produced allow an unambiguous discrimination of each of the four alphaherpesviruses related to BoHV-1. [less ▲]

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See detailImproved atomic data for iridium atom (Ir I) and ion (Ir II) and the solar content of iridium
Xu, H. L.; Svanberg, S.; Quinet, Pascal ULg et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (2007), 104(1), 52-70

From a combination of radiative lifetime measurements, performed with a time-resolved laser-induced fluorescence technique, for nine odd-parity levels of Ir I and four odd-parity levels of Ir 11 and ... [more ▼]

From a combination of radiative lifetime measurements, performed with a time-resolved laser-induced fluorescence technique, for nine odd-parity levels of Ir I and four odd-parity levels of Ir 11 and branching fraction calculations, extended sets of transition probabilities have been derived for transitions of astrophysical interest. The new results are compared with the few experimental data available in the literature and a good agreement is found. They extend considerably the previous sets of transition probabilities available in the literature. Implications of the new results regarding the solar content of iridium are briefly discussed. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailImproved automatic calibration of groundwater flow models using self-potential measurements
Robert, Tanguy ULg; Therrien, René; Lemieux, Jean-Michel et al

Conference (2011, April 11)

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See detailImproved blood pressure control but similar efficacy with lumiracoxib 100 mg OD compared to ibuprofen 600 mg TID in hypertensive patients with osteoarthritis: randomised controlled trial
MacDonald, T.; Littlejohn, T.; Richard, D. et al

in Annals of the Rheumatic Diseases (2007, June), 66(Suppl.II), 502

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See detailImproved blood pressure control with lumiracoxib compared with ibuprofen: a randomized trial in osteoarthritis patients with controlled hypertension
MacDonald, Thomas M; Reginster, Jean-Yves ULg; Littlejohn, Thomas W et al

in Arthritis and Rheumatism (2007, September), 56(number 9 (suppl.)), 659

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See detailImproved Carotid and Mesenteric Blood Flows with the Use of 21% Oxygen During Resuscitation of Newborn Piglets with Severe Asphyxia
Haase, Erika; Stevens, Jonathan; Rigo, Vincent ULg et al

in Pediatric Research (2003, April), 53(4 Part 2/2), 377

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See detailAn improved clean-up strategy for simultaneous analysis of polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF), and polychlorinated biphenyls (PCB) in fatty food samples
Pirard, Catherine ULg; Focant, Jean-François ULg; De Pauw, Edwin ULg

in Analytical and Bioanalytical Chemistry (2002), 372(2), 373-381

The study and extension of a simple automated clean-up method for polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) to a broad range of polychlorinated biphenyls (PCB) is ... [more ▼]

The study and extension of a simple automated clean-up method for polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) to a broad range of polychlorinated biphenyls (PCB) is described. The isolation of seven PCDD, ten PCDF, and three coplanar PCB (cPCB) is extended to eight monoortho substituted PCB and seven so-called "marker PCB" (Aroclor 1260) for fatty food samples. This enables quantification of 35 compounds - including all congeners with a WHO toxic equivalent factor (TEF)--in a single extraction and single purification step. The chromatographic behaviour of mono-ortho PCB and marker PCB on a variety of adsorbents, including basic alumina, has been studied. Partitioning of analytes through multi-column sequences is described and correlated with their structural and electronic properties, by use of molecular modelling calculations. The fractionation process available with the Power-Prep automated clean-up system enables rapid independent analysis of the different groups of compounds. Gas chromatography with high resolution mass spectrometry (GC-HRMS) is used for the PCDD/F and cPCB fraction and quadrupole ion-storage tandem in time mass spectrometry (GC-QISTMS) for analysis of the remaining PCB. A comparison study was performed on quality-control samples and real fatty food samples to evaluate the robustness of the new strategy compared with a reference method. On the basis of this simultaneous clean-up, a rapid simplified strategy for PCDD/F and selected PCB analysis determination is proposed for fatty food samples. [less ▲]

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See detailImproved coloration contrast and electrochromic efficiency of tungsten oxide films thanks to a surfactant-assisted ultrasonic spray pyrolysis process
Denayer, Jessica ULg; Aubry, Philippe; Bister, Geoffroy et al

in Solar Energy Materials & Solar Cells (2014), 130

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See detailImproved computer-assisted analysis of the global lymphatic network in human cervical tissues.
Balsat, Cédric ULg; Signolle, Nicolas; GOFFIN, Frédéric ULg et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (2014), 27(6), 887-98

Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters ... [more ▼]

Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters requires an objective characterization of the lymphatic vasculature. Here, we performed a global analysis of the lymphatic network using a new computerized method applied on whole uterine cervical digital images. Sixty-eight cases of cervical neoplasia (12 CIN3, 10 FIGO stage 1A and 46 stage IB1) and 10 cases of normal cervical tissue were reacted with antibodies raised against D2-40, D2-40/p16 and D2-40/Ki67. Immunostained structures were automatically detected on whole slides. The lymphatic vessel density (D2-40), proliferating lymphatic vessel density (D2-40/ki67) and spatial lymphatic distribution in respect to the adjacent epithelium were assessed from normal cervix to early cervical cancer and correlated with lymphovascular space invasion and lymph node status. Prominent lymphatic vessel density and proliferating lymphatic vessel density are detected under the transformation zone of benign cervix and no further increase is noted during cancer progression. Notably, a shift of lymphatic vessel distribution toward the neoplastic edges is detected. In IB1 cervical cancer, although intra- and peritumoral lymphatic vessel density are neither correlated with lymphovascular space invasion nor with lymph node metastasis, a specific spatial distribution with more lymphatic vessels in the vicinity of tumor edges is predictive of lymphatic dissemination. Herein, we provide a new computerized method suitable for an innovative detailed analysis of the lymphatic network. We show that the transformation zone of the benign cervix acts as a baseline lymphangiogenic niche before the initiation of neoplastic process. During cancer progression, this specific microenvironment is maintained with lymphatic vessels even in closer vicinity to tumor cells.Modern Pathology advance online publication, 6 December 2013; doi:10.1038/modpathol.2013.195. [less ▲]

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See detailAn Improved Constitutive Model for the Numerical Simulation of Semi-Solid Thixoforming
Koeune, Roxane ULg; Ponthot, Jean-Philippe ULg

in Proceedings of ACOMEN’2008, Advanced Computational Methods in Engineering (2008)

In order to model thixoforming processes, previous papers presented a thermo-mechanical one-phase modelling. This first version of constitutive model revealed several limitations: the model could not ... [more ▼]

In order to model thixoforming processes, previous papers presented a thermo-mechanical one-phase modelling. This first version of constitutive model revealed several limitations: the model could not degenerate properly to pure solid or liquid behaviour neither to free solid suspensions. The aim of this paper was to propose solutions to overcome these limitations. [less ▲]

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See detailAn improved constitutive model for the numerical simulation of semi-solid thixoforming
Koeune, Roxane ULg; Ponthot, Jean-Philippe ULg

in Journal of Computational & Applied Mathematics (2010), 234

In order to model thixoforming processes, previous papers presented a thermomechanical one-phase modelling. This first version of the constitutive model revealed several limitations: the model could not ... [more ▼]

In order to model thixoforming processes, previous papers presented a thermomechanical one-phase modelling. This first version of the constitutive model revealed several limitations: the model could not degenerate properly to pure solid or liquid behavior nor to free solid suspensions. The aim of this paper was to propose solutions to overcome these limitations. [less ▲]

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See detailImproved detection of chromosomal abnormalities in chronic lymphocytic leukemia by conventional cytogenetics using CpG oligonucleotide and interleukin-2 stimulation: A Belgian multicentric study.
Put, Natalie; Konings, Peter; Rack, Katrina et al

in Genes, Chromosomes & Cancer (2009), 48(10), 843-53

We performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic ... [more ▼]

We performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic leukemia (CLL) referred for routine analysis either at the time of diagnosis (n = 172) or during disease evolution (n = 45). Parallel cultures of peripheral blood or bone marrow were set up with the addition of either the conventional B-cell mitogen 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or a combination of CpG oligonucleotide (CpG) and interleukin-2 (IL-2). Cytogenetic analyses were performed on both cultures. Clonal abnormalities were identified in 116 cases (53%). In 78 cases (36%), the aberrant clone was detected in both cultures. Among these, the percentages of aberrant metaphases were similar in both conditions in 17 cases, higher in the CpG/IL-2 culture in 43 cases, and higher in the TPA culture in 18 cases. Clonal aberrations were detected in only one culture, either in CpG/IL-2 or TPA in 33 (15%) and 5 (2%) cases, respectively. Taken together, abnormal karyotypes were observed in 51% with CpG/IL-2 and 38% with TPA (P < 0.0001). Application of FISH (n = 201) allowed the detection of abnormalities not visible by conventional cytogenetic analysis in 80 cases: del(13q) (n = 71), del(11q) (n = 5), +12 (n = 2), del(14q) (n = 1), and del(17p) (n = 1). In conclusion, our results confirm that CpG/IL-2 stimulation increases the detection rate of chromosomal abnormalities in CLL compared with TPA and that further improvement can be obtained by FISH. However, neither conventional cytogenetics nor FISH detected all aberrations, demonstrating the complementary nature of these techniques. [less ▲]

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See detailImproved District Heating Network Operation by the Integration of High Temperature Heat Pumps
Sartor, Kevin ULg; Lemort, Vincent ULg; Dewallef, Pierre ULg

in Digital proceedings of the 8th Conference on Sustainable Development of Energy, Water and Environment Systems - SDEWES (2013, September 23)

Biomass Combined Heat and Power (CHP) plants connected to district heating networks are a very good opportunity to increase the share of renewable sources into energy systems. Frequently, important ... [more ▼]

Biomass Combined Heat and Power (CHP) plants connected to district heating networks are a very good opportunity to increase the share of renewable sources into energy systems. Frequently, important consumers are connected to ensure a stable base demand of heat throughout the year but they often have higher requirements in terms of temperature (i.e., steam) which involves high level of temperatures in the district heating network during the whole year and high levels of heat losses in the network. This contribution presents the possibility to decrease the level of temperature at which the district heating network operates and to use high temperature heat pumps connected locally at the consumption point to produce steam when needed. An investigation of the global design and integration through thermodynamic simulation models is realized for the University of Liège district heating network. This study is intended to determine the heat pump coefficient of performance and, therefore, to assess the balance between the savings in terms of heat losses and the additional heat pump electricity consumption. [less ▲]

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See detailImproved Domain Decomposition Method for the Helmholtz Equation
Thierry, Bertrand ULg; Antoine, X.; Boubendir, Y. et al

in Proceedings of the 21th International Conference on Domain Decomposition Methods (DD21) (2012, June)

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See detailImproved Domain Decomposition Method for the Helmholtz Equation"
Geuzaine, Christophe ULg; Antoine, X.; Boubendir, Y. et al

Scientific conference (2012, January 23)

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See detailImproved Domain Decomposition Method for the Wave Equation in Harmonic Regime
Geuzaine, Christophe ULg; Antoine, X.; Boubendir, Y. et al

in Proceedings of the 6th Advanced Computational Electromagnetics workshop (ACE 2012) (2012, March)

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See detailAn improved ELISA technique for the diagnosis of Psoroptes ovis infestations in cattle
Lonneux, J. F.; Mignon, Bernard ULg; Bossaert, K. et al

Poster (2005)

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See detailImproved emulsifying and foaming of whey proteins after enzymic fat hydrolysis
Blecker, Christophe ULg; Paquot, Michel ULg; Lamberti, I. et al

in Journal of Food Science (1997), 62(1), 48-

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This ... [more ▼]

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This enzymatic lipolysis would convert almost all triglycerides into mono- and diglycerides which have strong surface-active properties. Therefore, the emulsifying and foaming capacities of such modified whey products should be increased in comparison with native products, as shown by results presented at both neutral pH and those similar to a salad dressing emulsion. It was also demonstrated that emulsions and foams formed with lipase-treated whey preparations were more shelf-stable. [less ▲]

Detailed reference viewed: 15 (5 ULg)