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See detailIpriflavone: Pharmacological Properties and Usefulness in Postmenopausal Osteoporosis
Reginster, Jean-Yves ULg

in Bone and Mineral (1993), 23(3), 223-32

Ipriflavone (IP) is an isoflavone derivative available in several countries for investigational and/or therapeutic use. Inhibition of bone resorption was demonstrated in several models, both in vitro and ... [more ▼]

Ipriflavone (IP) is an isoflavone derivative available in several countries for investigational and/or therapeutic use. Inhibition of bone resorption was demonstrated in several models, both in vitro and in vivo for IP and its metabolites. Their mechanisms of action on bone are not yet fully elucidated but some of them are widely accepted. IP does not possess, per se, any estrogenic activity. It appears that IP-related inhibition of bone resorption might be mediated by an indirect effect on osteoclast and related to an inhibition of recruitment and/or differentiation of pre-osteoclast, maybe through a modulation of osteoblast response to PTH. Clinical studies in Paget's disease of bone or primary hyperparathyroidism have confirmed preferential inhibition of bone resorption suggesting a clinical interest in postmenopausal osteoporosis. Preliminary (1 year) results of double blind placebo controlled studies designed in postmenopausal and senile osteoporosis confirm a reduction in bone turnover rate in patients treated with 600 mg/day of IP, resulting in a significant bone-sparing effect both at lumbar and radial levels. All clinical and pharmacological trials confirm a very good tolerance of IP with a frequency of adverse reactions equal to that observed during administration of a placebo. Providing ongoing studies will confirm the actual promising preliminary results, IP seems a very interesting new non hormonal approach for prevention and treatment of postmenopausal and senile osteoporosis. [less ▲]

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See detailIPSAS in a nutshell - from principles to practice
Schumesch, Patrice ULg; De Laet, Jan; De Greef, Anton

Book published by PwC (2012)

Whilst there are calls for adopting high-quality, transparent and international accounting rules for governments and other public sector entities, many government officials and public sector accountants ... [more ▼]

Whilst there are calls for adopting high-quality, transparent and international accounting rules for governments and other public sector entities, many government officials and public sector accountants are not familiar with the requirements of IPSAS (International Public Sector Accounting Standards) and with the often substantial changes that adoption of these rules may imply, mainly for those entities that use cash accounting. Consequently they are not fully aware of the implications of applying IPSAS for their own organisation. The book aims to create an awareness of IPSAS; it avoids reproducing exhaustively the IPSAS texts but rather seeks to highlight the main accounting issues and principles, and explains how to apply them in practice. The objective is to be didactic and easy to read. Throughout the book, comments are inserted to provide practical guidance for professionals who work or may work with IPSAS as preparers or users of financial statements. [less ▲]

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See detailIpsilateral motor responses to focal TMS in normal man and stroke patients.
ALAGONA G.; DELVAUX, Valérie ULg; Delwaide, Paul ULg et al

in Clinical Neurophysiology (2000), 111(Suppl. 1), 11512-05

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See detailIpsilateral motor responses to focal transcranial magnetic stimulation in healthy subjects and acute-stroke patients
Alagona, Giovanna; DELVAUX, Valérie ULg; GERARD, Pascale ULg et al

in Stroke (2001), 32(6), 1304-1309

BACKGROUND AND PURPOSE: Prevalence and characteristics of ipsilateral upper limb motor-evoked potentials (MEPs) elicited by focal transcranial magnetic stimulation (TMS) were compared in healthy subjects ... [more ▼]

BACKGROUND AND PURPOSE: Prevalence and characteristics of ipsilateral upper limb motor-evoked potentials (MEPs) elicited by focal transcranial magnetic stimulation (TMS) were compared in healthy subjects and patients with acute stroke. METHODS: Sixteen healthy subjects and 25 patients with acute stroke underwent focal TMS at maximum stimulator output over motor and premotor cortices. If present, MEPs evoked in muscles ipsilateral to TMS were analyzed for latency, amplitude, shape, and center of gravity (ie, preferential coil location to elicit them). In stroke patients, possible relationships between early ipsilateral responses and functional outcome at 6 months were sought. RESULTS: With relaxed or slightly contracting target muscle, maximal TMS over the motor cortex failed to elicit ipsilateral MEPs in the first dorsal interosseous (FDI) or biceps of any of 16 normal subjects. In 5 of 8 healthy subjects tested, ipsilateral MEPs with latencies longer than contralateral MEPs were evoked in FDI muscle (in biceps, 6 of 8 subjects) during strong (>50% maximum) contraction of the target muscle. In 15 of 25 stroke patients, ipsilateral MEPs in the unaffected relaxed FDI (in biceps, 6 of 25 stroke patients) were evoked by stimulation of premotor areas of the affected hemisphere. Their latencies were shorter than those that MEPs evoked in the same muscle by stimulation of the motor cortex of the contralateral unaffected hemisphere. Such responses were never obtained in normal subjects and were mostly observed in patients with subcortical infarcts. Patients harboring these responses had slightly better bimanual dexterity after 6 months. CONCLUSIONS: Ipsilateral MEPs obtained in healthy individuals and stroke patients have different characteristics and probably different origins. In the former, they are probably conveyed via corticoreticulospinal or corticopropriospinal pathways, whereas in the latter, early ipsilateral MEPs could originate in hyperexcitable premotor areas. [less ▲]

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See detailIpsilateral motor responses to focal transcranial magnetic stimulation in normal and stroke patients.
ALAGONA G.; DELVAUX, Valérie ULg; GERARD, Pascale ULg et al

in Journal of Neurology, Neurosurgery & Psychiatry (2000), Neurosurgery and Psychiatry

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See detailIpsilateral responses after a stroke : an electrophysiological study by focal TMS
Alagona, Giovanna; Rapisarda, giuseppe; DELVAUX, Valérie ULg

Poster (1999)

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See detailThe Iq motif defines the conformation of the interacting calmodullin
Rebowski; Terrak, Mohammed ULg; Lu, R. C. et al

in Biophysical Journal (2004, January), 86

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See detailIR and UV spectroscopic signatures of DNA higher-order structures in the gas phase
Gabelica, Valérie ULg; Rosu, Frédéric ULg; Gregoire, Gilles et al

Conference (2008, June 03)

Introduction Electrospray mass spectrometry (ESI-MS) can be used to transfer large biomolecular complexes from the solution to the gas phase. However, a longstanding question is whether the gas-phase ... [more ▼]

Introduction Electrospray mass spectrometry (ESI-MS) can be used to transfer large biomolecular complexes from the solution to the gas phase. However, a longstanding question is whether the gas-phase multiply-charged ions produced by ESI-MS keep a folded conformation in the absence of solvent. Nucleic acid secondary structures are determined by hydrogen bonding interactions between nucleic bases and by stacking interactions between neighboring base pairs. Here we will show that infrared (IR) and ultraviolet (UV) action spectroscopies provide useful and complementary information on the structure of nucleic acid ions in the gas phase. Methods IR spectroscopy experiments on DNA negative ions were carried out at the CLIO free electron laser (FEL) center (Orsay, France) using an Esquire 3000 (Bruker) mass spectrometer modified to inject the IR beam through the ring electrode. IRMPD spectra are recorded by monitoring the fragmentation of mass-selected parent ions as a function of the excitation wavenumber, in the range 1000-2000 cm-1. UV spectroscopy experiments were carried out using a tunable OPO laser (Continuum Lasers) with frequency doubling. The laser is interfaced with either a Finnigan LCQ ESI-QIT mass spectrometer or a Bruker Apex-Qe 9.4 T ESI-FTICR mass spectrometer. The UV action spectra were recorded by monitoring electron detachment as a function of the wavelength between 220 and 300 nm. Preliminary results First, DNA oligonucleotide ions forming G-quadruplex structures were studied in the gas phase using IR multiple-photon dissociation spectroscopy. Data interpretation on these large biomolecule ions is made using carefully chosen control experiments. The IR spectrum of the (dTG4T)4 quadruplex has been recorded, and compared to that of the single strand. Given the strand stoichiometry and the selective incorporation of three ammonium cations, there is little doubt about the quadruplex structure of [(dTG4T)4•(NH4+)3]5-. The major finding is a fingerprint of hydrogen bonding in the gas phase in the guanine C6=O6 stretching mode, that allows probing the conservation of G-quartets in the gas phase. Further experiments also demonstrate the conservation of G-quadruplex hydrogen bonds in the human telomeric sequence d(TTAGGG)4 [Gabelica et al., JACS, accepted]. Second, we also studied DNA duplexes and G-quadruplex ions in the gas phase by UV spectroscopy. We recorded the UV spectra of the (dTG4T)4 quadruplex, with and without ammonium ions. Molecular modeling [Rueda et al., JACS, 2006, p3608] and ion mobility spectrometry data [Gabelica et al., JACS, 2007, 895] showed that G-quadruplexes keep their hydrogen-bonded structure but become more floppy if inner cations are removed. We found that the UV spectra differ dramatically with and without inner cations, suggesting that UV spectroscopy is very sensitive to stacking interactions between neighboring G-quartets. We also used UV spectroscopy to probe the structure of 12-mer DNA duplexes, by comparing the duplex spectra to those obtained on single strands. Preliminary results show that stacking interactions may be preserved in duplexes containing GC base pairs, but not in duplexes containing AT base pairs. Altogether, these results show the complementarities between IR and UV spectroscopy to characterize DNA structures in the gas phase: IR data mainly give access to information on hydrogen bonding of bases, and UV spectroscopy provides information on stacking interactions. [less ▲]

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See detailIR and UV spectroscopic signatures of DNA structures in the gas phase
Gabelica, Valérie ULg

Conference (2009, July 08)

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See detailIR and UV spectroscopy of DNA ions stored in a quadrupole ion trap mass spectrometer
Gabelica, Valérie ULg; Rosu, Frédéric ULg; Gregoire, Gilles et al

Conference (2008, February 15)

Electrospray mass spectrometry (ESI-MS) can be used to transfer large biomolecular complexes from the solution to the gas phase. However, a longstanding question is whether the gas-phase multiply-charged ... [more ▼]

Electrospray mass spectrometry (ESI-MS) can be used to transfer large biomolecular complexes from the solution to the gas phase. However, a longstanding question is whether the gas-phase multiply-charged ions produced by ESI-MS keep a folded conformation in the absence of solvent. Nucleic acid secondary structures are determined by hydrogen bonding interactions between nucleic bases and by stacking interactions between neighboring base pairs. In solution, infrared (IR) and ultraviolet (UV) spectroscopies provide information on hydrogen bonding and stacking interactions in nucleic acids, respectively. Here we will show how IR and UV spectra of gas-phase ions can be recorded, and what can be learned on the structure of nucleic acids (double helices and quadruple helices) in the gas phase. The IR spectroscopy experiments on DNA negative ions were carried out at the CLIO free electron laser (FEL) center using an electrospray quadrupole ion trap mass spectrometer (Esquire 3000, Bruker Daltonics, Germany) modified to inject the IR beam in the trap through the ring electrode. IRMPD spectra are recorded by monitoring the relative fragmentation efficiency of mass-selected parent ions as a function of the excitation wavenumber, in the range 1000-2000 cm-1. Data interpretation on these large biomolecule ions is made using carefully chosen control experiments. The major finding is a fingerprint of hydrogen bonding in the gas phase in the guanine C6=O6 stretching mode, that allows probing the conservation of G-quartets in the gas phase. The experiments demonstrate the conservation of G-quadruplex hydrogen bonds in the human telomeric sequence d(TTAGGG)4. The UV spectroscopy experiments were carried out using a tunable OPO laser (Continuum Lasers, Santa Clara, CA, USA) with frequency doubling. The laser is interfaced with a Finnigan LCQ ESI-QIT mass spectrometer. The UV action spectra were recorded by monitoring electron detachment from DNA multiply charged anions as a function of the wavelength between 220 and 300 nm. Preliminary results suggest that stacking interactions are preserved in duplexes containing GC base pairs, and in G-quadruplexes containing inner cations. [less ▲]

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See detailIR and UV Spectroscopy of DNA Ions Stored in Trap Instruments
Gabelica, Valérie ULg

Conference (2008, October 11)

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See detailIR photometry of ESO calibration stars (van der Bliek+ 1996)
van der Bliek, N. S.; Manfroid, Jean ULg; Bouchet, P.

Report (1996)

We describe the infrared (IR) photometric system for the single channel photometers at ESO, which have been used from 1983 until 1994. In addition to the broadband near infrared (NIR, 1-5μm) photometric ... [more ▼]

We describe the infrared (IR) photometric system for the single channel photometers at ESO, which have been used from 1983 until 1994. In addition to the broadband near infrared (NIR, 1-5μm) photometric system presented in 1991 by Bouchet et al. and Bersanelli et al., we describe a narrow-band NIR photometric system and a mid infrared (MIR, 7-20μm) photometric system. We also extend the set of NIR standard stars by Bouchet et al. towards fainter objects (K=~9). The photometric data of the standard stars in these systems were extracted from the complete IR photometric data archive of ESO, covering 10 years. The zeropoints of the NIR photometry are set by assuming that HR 3314 has a V-magnitude of 3.89, and that V-K=-0.05, J-K=-0.01, H-K=-0.01, K-L'=0.00, K-M=0.00. The zeropoints of the MIR photometry are set by assuming that the colours ofβ Hyi (HR 0098) and α CenA (HR 5459) are equal to the colours of the Sun. We adopt the absolute calibration of Megessier (1995A&A...296..771M) for the NIR and we argue that this calibration can be extrapolated to 20μm, using the MIR calibrations by Rieke et al. (1985AJ.....90..900R) and Cohen et al. (1992AJ....104.1650C). The definition of the zeropoints is consistent with the absolute calibration. We obtained accurate (Ï =~0.02mag.) NIR photometry of about 240 standard stars and MIR photometry of about 40 standard stars (Ï =~0.04mag). Comparison of our NIR photometric system with other well established systems shows that there are some small colour dependencies and zeropoint offsets which are always smaller than about 0.02mag. except for the L' band. (3 data files). [less ▲]

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See detailIR spectroscopy of DNA structures
Gabelica, Valérie ULg

Conference (2009, June 11)

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See detailIr-LBP, an ixodes ricinus tick salivary LTB4-binding lipocalin, interferes with host neutrophil function.
Beaufays, Jérôme ULg; Adam, Benoit; Menten-Dedoyart, Catherine ULg et al

in PLoS ONE (2008), 3(12), 3987

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation ... [more ▼]

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. METHODOLOGY/PRINCIPAL FINDINGS: We previously identified 14 new lipocalin genes in the tick Ixodes ricinus. One of them codes for a protein that specifically binds leukotriene B4 with a very high affinity (Kd: +/-1 nM), similar to that of the neutrophil transmembrane receptor BLT1. By in silico approaches, we modeled the 3D structure of the protein and the binding of LTB4 into the ligand pocket. This protein, called Ir-LBP, inhibits neutrophil chemotaxis in vitro and delays LTB4-induced apoptosis. Ir-LBP also inhibits the host inflammatory response in vivo by decreasing the number and activation of neutrophils located at the tick bite site. Thus, Ir-LBP participates in the tick's ability to interfere with proper neutrophil function in inflammation. CONCLUSIONS/SIGNIFICANCE: These elements suggest that Ir-LBP is a "scavenger" of LTB4, which, in combination with other factors, such as histamine-binding proteins or proteins inhibiting the classical or alternative complement pathways, permits the tick to properly manage its blood meal. Moreover, with regard to its properties, Ir-LBP could possibly be used as a therapeutic tool for illnesses associated with an increased LTB4 production. [less ▲]

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See detailIR-visible sum-frequency vibrational spectroscopy of Biphenyl-3 methylene thiol monolayer on gold and silver: effect of the visible wavelength on the SFG spectrum
Humbert, Christophe; Dreesen, Laurent ULg; Mani, A. A. et al

in Surface Science (2002), 502-503

We measured IR-visible sum-frequency generation spectra of CH3-(C6H4)2-(CH2)3-S-H (Biphenyl-3) self-assembled monolayers on a silver and a gold substrate. For the latter substrate, we observed different ... [more ▼]

We measured IR-visible sum-frequency generation spectra of CH3-(C6H4)2-(CH2)3-S-H (Biphenyl-3) self-assembled monolayers on a silver and a gold substrate. For the latter substrate, we observed different interference patterns between the resonant signal of the CH vibration and the non-resonant contribution of the substrate as a function of the visible beam wavelength. The non-linear response of the gold substrate is enhanced around 480 nm corresponding to the s-d interband transition. Such effect is not observed for the silver substrate the interband transition of which is located out of the investigated visible spectral range of 450-700 nm. [less ▲]

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See detailIRC +10216: a peanut nebula!
Le Bertre, Thibault; Magain, Pierre ULg; Remy, Marc

in The Messenger (1989), 55

Not Available

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See detailIRF-3 dependent activation of inflammatory dendritic cells by extracellular host DNA mediates the adjuvant activity of alum on Th2 responses
Marichal, Thomas ULg; Ohata, Keichii; Bedoret, Denis et al

in Proceedings of the 25th annual meeting of the European Macrophage and Dendritic Cell Society (2011)

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