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See detailIsolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Bayane, Ali; Diawara, B.; Dubois Dauphin, Robin ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

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See detailIsolation and characterisation of seven alien monosomic addition lines of Gossypium australe F. Muell. on G. hirsutum L.
Ahoton, L.; Lacape, J. M.; D'Hont, A. et al

Conference given outside the academic context (2004)

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See detailIsolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Reproduction Nutrition Development (2004), 44(3, May-Jun), 169-181

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the ... [more ▼]

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the present study was to isolate as many PAG molecules as possible from placentae collected between day 60 and day 100 of gestation and to characterize their amino-terminal amino-acid sequences. Three heterologous radioimmunoassays were used to monitor PAG immunoreactivity throughout the isolation procedures. Sequential use of DEAE-cellulose, gel filtration, and CM ceramic chromatographies led to the isolation of several fractions rich in PAG immunoreactivity. The fractions with a large amount of proteins were also purified by chromatofocusing. The analysis of immunoreactive fractions by SDS-PAGE, Western blotting and two-dimensional electrophoresis followed by amino-terminal microsequencing on PVDF membranes allowed to identify eight different ovPAG with apparent molecular masses ranging from 55 to 66 kDa and isoelectric points from 4.0 to 6.8. The N-terminal sequences were determined and their comparison to those previously identified revealed that four of them are identical to those encoded by previously known cDNA, while the additional four sequences appear to be novel since they have not yet been described. [less ▲]

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See detailThe isolation and characterization of equine Osteocalcin
Carstanjen, B; Wattiez, R; Amory, Hélène ULg et al

in Proceedings of the 1st meeting of the International Bone and Mineral Society (IBMS) (2001)

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See detailIsolation and characterization of photoactive complexes of NADPH : protochlorophyllide oxidoreductase from wheat
Chahdi, M. A. O.; Schoefs, B.; Franck, Fabrice ULg

in Planta (1998), 206(4), 673-680

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of ... [more ▼]

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of prolamellar bodies by dodecyl-maltoside. Irradiation by a 1-ms flash induced the phototransformation of protocholorophyllide a (Pchlide) with -196 degrees C absorbance and emission maxima at 640 and 643 nm, respectively. The apparent molecular weight of this complex was 112 +/- 24 kDa, which indicates aggregation of enzyme subunits. By lowering the detergent concentration in the elution buffer, a 1080 +/- 250-kDa particle was obtained which displayed the spectral properties of the predominant form of photoactive Pchlide in vivo (-196 degrees C absorbance and fluorescence maxima at 650 and 653 nm). In this complex, FOR was the dominant polypeptide. Gel chromatography in the same conditions of an irradiated sample of solubilized prolamellar bodies indicated rapid disaggregation of the complex after Pchlide phototransformation. High performance liquid chromatographic analysis of the FOR complexes obtained using two detergent concentrations indicates a possible association of zeaxanthin and violaxanthin with the photoactive complex. [less ▲]

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See detailIsolation and characterization of preantral follicles from fetal bovine ovaries
Hulshof, S. C. J.; Figueiredo, José Ricardo; Beckers, Jean-François ULg et al

in Veterinary Quarterly (The) (1994), 16(2), 78-80

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The ... [more ▼]

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter. [less ▲]

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See detailIsolation and characterization of respiratory NADH:ubiquinone oxidoreductase (complex I) mutants in Chlamydomonas reinhardtii
Remacle, Claire ULg; Baurain, Denis ULg; Colin, Martine et al

in Archives of Physiology & Biochemistry (2000), 108(Supplement 1), 10

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See detailIsolation and characterization of the human prolactin gene
Truong, Anh T; Duez, Colette ULg; Belayew, Alexandra et al

in EMBO Journal (1984), 3(2), 429-37

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of ... [more ▼]

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of the many hormones acting on them. This provides an interesting system to study sequences involved in gene expression. Using a human PRL cDNA clone as a probe, we screened a human genomic DNA library in lambda phage and isolated a single recombinant comprising the whole hPRL gene. It was characterized by restriction endonuclease mapping and cDNA hybridization, by DNA heteroduplex analysis and by nucleotide sequencing. The hPRL gene is present as a single copy per haploid genome, is approximately 10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes. The origin of transcription was determined by S1 mapping on prolactinoma mRNAs. The search for direct and inverted repeats, as well as dyad symmetries was carried out in the 900-bp sequenced in the 5'-flanking region. Sequence homologies between hPRL, hGH and rPRL were derived from computer drawn matrices for these upstream regions. [less ▲]

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See detailIsolation And Chemical Evaluation Of Carob (Ceratonia Siliqua L.) Seed Germ
Dakia, Patrick; Wathelet, Bernard ULg; Paquot, Michel ULg

in Food Chemistry (2007), 102(4),

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See detailIsolation and Cultivation of a Xylanolytic Bacillus subtilis Extracted from the Gut of the Termite Reticulitermes santonensis
Tarayre, Cédric ULg; Brognaux, Alison ULg; Brasseur, Catherine ULg et al

in Applied Biochemistry and Biotechnology (2013)

The aim of this work was the isolation of xylanolytic microorganisms from the digestive tract of the termite Reticulitermes santonensis. The reducing sugars released after the hydrolysis of xylans can be ... [more ▼]

The aim of this work was the isolation of xylanolytic microorganisms from the digestive tract of the termite Reticulitermes santonensis. The reducing sugars released after the hydrolysis of xylans can be further fermented to provide bioethanol. A xylanolytic strain of Bacillus subtilis was isolated from the hindgut of the termite and displayed amylase and xylanase activities. The bacterium was grown on media containing agricultural residues: wheat bran, wheat distiller’s grains, and rapeseed oil cake. Wheat bran led to the highest induction of xylanase activity, although the development of the strain was less fast than in the other media. It was possible to reach maximal xylanase activities of 44.3, 33.5, and 29.1 I.U./ml in the media containing wheat bran, wheat distiller’s grains, and rapeseed oil cake, respectively. Mass spectrometry identified a wide range of xylose oligomers, highlighting an endoxylanase activity. The enzyme was stable up to 45 °C and displayed an optimal pH close to 8. [less ▲]

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See detailIsolation and cultivation of cellulolytic and xylanolytic bacteria and molds extracted from the gut of the termite Reticulitermes santonensis (3DV.1.14)
Tarayre, Cédric ULg; Bauwens, Julien ULg; Mattéotti, Christel et al

Poster (2013, June)

Biofuel production can be based on the use of agro-residues, consisting in a complex lignocellulosic structure which is not easily hydrolysable. The digestive tract of the termite Reticulitermes ... [more ▼]

Biofuel production can be based on the use of agro-residues, consisting in a complex lignocellulosic structure which is not easily hydrolysable. The digestive tract of the termite Reticulitermes santonensis contains a diversified microflora able to hydrolyze the wood components. Bacteria, molds and protists form efficient consortia, able to break the lignocellulosic complex by producing enzymes, such as xylanases and cellulases. Our purpose is the isolation of microbial strains from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. Termites were fed using different diets chosen to improve the xylanolytic and cellulolytic microflora: wood, microcristalline cellulose (added with lignin or not), α-cellulose (added with lignin or not) and birchwood xylan. Then, dissections were realized to isolate the potential xylanolytic and cellulolytic strains. This approach led us to isolate and to study several strains of bacteria (Bacillus sp. strain CTGx and Chryseobacterium sp. strain CTGx) and molds (Trichoderma virens strain CTGx and Sarocladium kiliense strain CTGx). These microorganisms were able to hydrolyze starch, xylan, cellulose, carboxymethylcellulose, esculin, β-glucan and Whatman® filter paper. They can produce glucose and xylose monomers and oligomers which can be further fermented to produce bioethanol. [less ▲]

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See detailIsolation and evaluation of bacteria and fungi as biological control agents against Rhizoctonia solani.
Lahlali, R.; Bajii, M.; Jijakli, Mohamed ULg

in Communications in agricultural and applied biological sciences (2007), 72(4), 973-982

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See detailIsolation and identification of a new Bacillus strain for amylase production
Bakri, Y.; Ammouneh, H.; El-Khouri, S. et al

in Research in Biotechnology (2012), 3(6), 51-58

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See detailIsolation and identification of a new fungal strain for amylase biosynthesis
Bakri, Y.; Masson, M.; Thonart, Philippe ULg

in Polish Journal of Microbiology (2009), 58(3), 269-273

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See detailIsolation and identification of antioxidant phytochemicals from Cuban species of the genera Erythroxylum P. Browne and Pluchea Cass
Perera Cordova, Wilmer ULg

Doctoral thesis (2012)

Phytochemicals showing antioxidant properties are largely recognized as beneficial to human health and disease prevention. Cuba is well known for having a rich flora with a high percentage of endemic ... [more ▼]

Phytochemicals showing antioxidant properties are largely recognized as beneficial to human health and disease prevention. Cuba is well known for having a rich flora with a high percentage of endemic species; it is considered as an interesting source of plant species for searching bioactive metabolites. Polar and non-polar extracts were prepared by fractionation from macerations in ethanol: H2O (7:3 v/v) from several Cuban plant species. The antioxidant capacity and the concentration in phenolic compounds were assayed in these extracts. The native species Pluchea carolinensis (Jacq.) G. Don., Pluchea odorata (L.) Cass. and Pluchea rosea Godfrey were identified as the most promising sources of antioxidants. The n-butanol extracts obtained by reflux from endemic Erythroxylum alaternifolium A. Rich. var. alaternifolium, var. parvifolium and suborbiculare also displayed high antioxidant capacity and high levels in phenolic compounds. The antioxidant capacity and the phenolic content of hydroalcoholic macerations from leaves, inflorescences, stems and roots of species of the genus Pluchea were also compared. Leaf extracts followed by inflorescence extracts showed the highest values of antioxidant capacity. The species P. carolinensis was used as a model to evaluate the antioxidant capacity in two locations and two phenological stages and also for monitoring the antioxidant capacity over some months. Natural adult specimens presented a higher phenolic content, as well as higher antioxidant capacity than young and cultivated specimens. Maximal antioxidant capacity and concentrations in phenolics were recorded in January, September and December; over the blooming stage (March), the antioxidant capacity was minimal. Two different solvents of extraction and two methods were also screened for extracting the maximal concentrations in antioxidants. Hydroalcoholic extractions showed higher antioxidant capacity than ethanolic ones. However, no difference in antioxidant capacity were measured between the two methods of extraction. The antioxidant capacity of P. carolinensis was also measured in leaves of plantlets micro-propagated on three different in vitro culture media and it was compared with leaves of young specimens grown ex-vitro. The inclusion of a cytokinin in culture media increased the antioxidant capacity of the leaf extracts of plantlets grown in vitro. However, the antioxidant capacity of plantlets grown in vitro was lower than that of young specimens grown ex-vitro. Additionally, several phenolic compounds were isolated from the species studied. Two flavonol glycosides were isolated from n-butanol leaf extract of Erythroxylum alaternifolium var. alaternifolium. Moreover, 22 phenolic compounds (including 9 phenolic acids and 13 flavonoids) were also identified from leaf, inflorescence and stem extracts of the three Pluchea species. All of them are reported for the first time in Cuban Pluchea species. In addition, rosmarinic acid, ferulic acid, quercetagetin, herbacetin, quercitrin, eupalitin and 3-methyl-quercetagetin were described for the first time in the genus Pluchea. The antioxidant capacity of each of these compounds was evaluated. The results suggested that most of these phytochemicals have an important contribution to the antioxidant capacity found in plant extracts. [less ▲]

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See detailIsolation and identification of dominant osmophilic Leuconostoc strains from traditional date product “Btana”
Abekhti, Abdelkader; Daube, Georges ULg; Kihal, M.

in International Food Research Journal [=IFRJ] (2014), 21(4), 1261-1268

The current study aimed to isolate and identify dominant osmophilic bacteria associated with a traditional date product named “Btana”, produced in south region of Maghreb countries. Samples were randomly ... [more ▼]

The current study aimed to isolate and identify dominant osmophilic bacteria associated with a traditional date product named “Btana”, produced in south region of Maghreb countries. Samples were randomly collected after two month of storage from tow villages (Mtarfat and Abani) in the Algerian southern department “Adrar”. A high osmotic pressure medium (MSE) was used for isolation of osmophilic bacteria, which were purified and examined for macroscopic and microscopic shape, Gram stain, catatalse, oxydase, acetoine and ADH production, reduction of nitrate, and motility. Isolates were then subculture on MRS medium for production of dextran, gas from glucose, growth in the presence of NaCl (3, 6.5 %) and sucrose (10, 20, 30, 40, and 50 %), pH tolerance (4.8, 6.5), growth temperature (10, 37, and 45°C) and thermo resistance (55°C for 15 min), enzymatic activity (proteolytic, lipolytic, hemolysis). Isolates were identified to specie’s level by sugar fermentation. Their growth and acidification kinetic were also studied. Results identified two species of Leuconostoc; Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc mesenteroides subsp. dextranicum. They show a high antibacterial activity against four indicator bacteria; Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. [less ▲]

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