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See detailIsolated chlamydia trachomatis infection and cervical intraepithelial neoplasia in adolescence
To, Hong ULg

in The 2012 Conference of Asia Oceania Research Organisation on Genital Infection and Neoplasia, Hongkong 13-15 July 2012 (2012, July 15)

Many authors suggest an association between CT infection and dysplasia or neoplastic cervical atypia, but evidence of an isolated Chlamydia trachomatis (CT) infection increasing the risk of developing ... [more ▼]

Many authors suggest an association between CT infection and dysplasia or neoplastic cervical atypia, but evidence of an isolated Chlamydia trachomatis (CT) infection increasing the risk of developing cervical intraepithelial neoplasia (CIN) remains unproven. We present a case of isolated CT infection in an adolescent 17 year old girl who was referred to our hospital with high grade cervical squamous intraepithelial lesion (HSIL) on her Pap smear. CT was detected in both the rapid test of the endocervix specimen and PCR testing of urine, but human papilloma virus (HPV) infection (Qualitative HPV PCR test) was not present. CIN3 was confirmed on biopsy of the thick acetowhite and mosaic zone at colposcopy. Isolated CT infection is a potential factor in the development of CIN in adolescence. [less ▲]

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See detailIsolated systolic and diastolic dippers, an intermediate subgroup between dippers and non-dippers
Moonen, martial; Saint-Remy, Annie ULg; Krzesinski, Jean-Marie ULg et al

Conference (1997, October 04)

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See detailIsolated transient UV auroral structures at Jupiter: possible signatures of magnetospheric injections 
Dumont, Maïté ULg; Grodent, Denis ULg; Radioti, Aikaterini ULg et al

Conference (2013, July 11)

We investigate transient ultraviolet auroral features located equatorward of the main emission (130 features) based on Hubble Space Telescope (HST) observations of the northern and southern Jovian ... [more ▼]

We investigate transient ultraviolet auroral features located equatorward of the main emission (130 features) based on Hubble Space Telescope (HST) observations of the northern and southern Jovian hemispheres (2000-2007). Several properties of the auroral emissions are analyzed, such as their position in auroral region, power and brightness. Additionally, we magnetically map the auroral structures to the equatorial plane using VIPAL model and we compare their observed properties with those of magnetospheric injections observed by Galileo. We suggest that these transient auroral structures could be related to magnetospheric injections. The mapped radial position and system III longitude of the observed auroral features are in good agreement with those of the injections observed in the equatorial plane by Galileo. Based on power and brightness of the auroral features, we discuss the mechanisms involved in the ionosphere-magnetosphere coupling injections. This comparative study demonstrates that the structures under study are related to magnetospheric injections and sheds light to the mechanism involved in the magnetosphere-ionosphere dynamics. [less ▲]

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See detailIsolating the 130.4 nm and 135.6 nm emissions in Ganymede’s aurora using broadband optics
Molyneux, Philippa M; Grodent, Denis ULg; Bunce, Emma J et al

Conference (2012, September 27)

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that ... [more ▼]

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that the ra- tio of OI emissions at 135.6 nm and 130.4 nm can be recovered if the transmission of the filters and other optical elements are well known. This ratio allows constraints to be placed on the relative abundances of O atoms and O2 molecules within Ganymede’s at- mosphere, leading to more accurate models of atmo- spheric composition. [less ▲]

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See detailIsolation ad Characterization of osteocalcin in horses
Carstanjen, B; Wattiez, R; Amory, Hélène ULg et al

in Proceedings of the ECVS meeting 2002 (Resident Forum) (2002)

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See detailIsolation and analysis of a putative FLC orthologue in Sinapis alba L.
D'Aloia, Maria ULg; Melzer, Siegbert; Périlleux, Claire ULg

in Acta Physiologiae Plantarum (2004), 26(3), 23-24

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to ... [more ▼]

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to flower the next summer, hence behave as biennials or winter crops. A recent breakthrough in the understanding of the molecular bases of vernalization has been the cloning of the FLOWERING LOCUS C (FLC) gene in Arabidopsis thaliana (Michaels S.D. & Amasino R.M., 1999). FLC encodes a repressor of flowering and is downregulated by vernalization. So vernalization relieves the inhibitory role that FLC plays on downstream genes involved in the floral transition of the shoot apical meristem (SAM). Although vernalization has been shown, by physiological studies, to be sensed by the SAM, molecular evidences are missing. Such analyses are impaired in Arabidopsis by the small size of the plant and the rosette growth habit. We therefore cloned a FLC homologue in a caulescent relative Brassicaceae: mustard (Sinapis alba L.). We identified two clones by screening a cDNA library made from leaf mRNA. One of them, SaFLC1, was used for in situ hybridizations on SAM sections. Preliminary results are shown. Because of its agronomical importance, we have also tried to manipulate the vernalization process in crops. By using a transgenic approach, we have attempted to bypass the strict vernalization requirement of winter colza (Brassica napus L.). We have over-expressed SaMADS A, a gene which is repressed by FLC in wild type, and observed that the transgenics did not require vernalization any more. [less ▲]

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See detailIsolation and biomass production of a Saccharomyces cerevisiae strain binding copper and zinc ions
Stroobants, Aurore ULg; Delroisse, Jean-Marc; Delvigne, Frank ULg et al

in Applied Biochemistry and Biotechnology (2009), 157(1),

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See detailIsolation and Characterisation of a Ruminant Alphaherpesvirus Closely Related to Bovine Herpesvirus 1 in a Free-Ranging Red Deer
Thiry, Julien ULg; Widen, F.; Grégoire, Fabien ULg et al

in BMC Veterinary Research (2007), 3

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine ... [more ▼]

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population. RESULTS: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1. CONCLUSION: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses. [less ▲]

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See detailIsolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations
Bayane, Ali; Diawara, B.; Dubois Dauphin, Robin ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

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See detailIsolation and characterisation of seven alien monosomic addition lines of Gossypium australe F. Muell. on G. hirsutum L.
Ahoton, L.; Lacape, J. M.; D'Hont, A. et al

Conference given outside the academic context (2004)

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See detailIsolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Reproduction Nutrition Development (2004), 44(3, May-Jun), 169-181

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the ... [more ▼]

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the present study was to isolate as many PAG molecules as possible from placentae collected between day 60 and day 100 of gestation and to characterize their amino-terminal amino-acid sequences. Three heterologous radioimmunoassays were used to monitor PAG immunoreactivity throughout the isolation procedures. Sequential use of DEAE-cellulose, gel filtration, and CM ceramic chromatographies led to the isolation of several fractions rich in PAG immunoreactivity. The fractions with a large amount of proteins were also purified by chromatofocusing. The analysis of immunoreactive fractions by SDS-PAGE, Western blotting and two-dimensional electrophoresis followed by amino-terminal microsequencing on PVDF membranes allowed to identify eight different ovPAG with apparent molecular masses ranging from 55 to 66 kDa and isoelectric points from 4.0 to 6.8. The N-terminal sequences were determined and their comparison to those previously identified revealed that four of them are identical to those encoded by previously known cDNA, while the additional four sequences appear to be novel since they have not yet been described. [less ▲]

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See detailThe isolation and characterization of equine Osteocalcin
Carstanjen, B; Wattiez, R; Amory, Hélène ULg et al

in Proceedings of the 1st meeting of the International Bone and Mineral Society (IBMS) (2001)

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See detailIsolation and characterization of photoactive complexes of NADPH : protochlorophyllide oxidoreductase from wheat
Chahdi, M. A. O.; Schoefs, B.; Franck, Fabrice ULg

in Planta (1998), 206(4), 673-680

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of ... [more ▼]

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of prolamellar bodies by dodecyl-maltoside. Irradiation by a 1-ms flash induced the phototransformation of protocholorophyllide a (Pchlide) with -196 degrees C absorbance and emission maxima at 640 and 643 nm, respectively. The apparent molecular weight of this complex was 112 +/- 24 kDa, which indicates aggregation of enzyme subunits. By lowering the detergent concentration in the elution buffer, a 1080 +/- 250-kDa particle was obtained which displayed the spectral properties of the predominant form of photoactive Pchlide in vivo (-196 degrees C absorbance and fluorescence maxima at 650 and 653 nm). In this complex, FOR was the dominant polypeptide. Gel chromatography in the same conditions of an irradiated sample of solubilized prolamellar bodies indicated rapid disaggregation of the complex after Pchlide phototransformation. High performance liquid chromatographic analysis of the FOR complexes obtained using two detergent concentrations indicates a possible association of zeaxanthin and violaxanthin with the photoactive complex. [less ▲]

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See detailIsolation and characterization of preantral follicles from fetal bovine ovaries
Hulshof, S. C. J.; Figueiredo, José Ricardo; Beckers, Jean-François ULg et al

in Veterinary Quarterly (The) (1994), 16(2), 78-80

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The ... [more ▼]

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter. [less ▲]

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See detailIsolation and characterization of respiratory NADH:ubiquinone oxidoreductase (complex I) mutants in Chlamydomonas reinhardtii
Remacle, Claire ULg; Baurain, Denis ULg; Colin, Martine et al

in Archives of Physiology & Biochemistry (2000), 108(Supplement 1), 10

Detailed reference viewed: 22 (11 ULg)
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See detailIsolation and characterization of the human prolactin gene
Truong, Anh T; Duez, Colette ULg; Belayew, Alexandra et al

in EMBO Journal (1984), 3(2), 429-37

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of ... [more ▼]

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of the many hormones acting on them. This provides an interesting system to study sequences involved in gene expression. Using a human PRL cDNA clone as a probe, we screened a human genomic DNA library in lambda phage and isolated a single recombinant comprising the whole hPRL gene. It was characterized by restriction endonuclease mapping and cDNA hybridization, by DNA heteroduplex analysis and by nucleotide sequencing. The hPRL gene is present as a single copy per haploid genome, is approximately 10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes. The origin of transcription was determined by S1 mapping on prolactinoma mRNAs. The search for direct and inverted repeats, as well as dyad symmetries was carried out in the 900-bp sequenced in the 5'-flanking region. Sequence homologies between hPRL, hGH and rPRL were derived from computer drawn matrices for these upstream regions. [less ▲]

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See detailIsolation And Chemical Evaluation Of Carob (Ceratonia Siliqua L.) Seed Germ
Dakia, Patrick; Wathelet, Bernard ULg; Paquot, Michel ULg

in Food Chemistry (2007), 102(4),

Detailed reference viewed: 35 (5 ULg)