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See detailImmunohistological Demonstration Of Virus And Tumor Associated Antigens In Tissues In Experimental And Spontaneous Bovine Leukemia-Virus (Blv) Infection
Reinacher, M.; Thurmond, Mc.; Onuma, M. et al

in Veterinary Immunology and Immunopathology (1989), 22(3),

Detailed reference viewed: 8 (0 ULg)
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See detailImmunohistological localization of three basement membrane components in various forms of epidermolysis bullosa.
Kero, M.; Peltonen, L.; Foidart, Jean-Michel ULg et al

in Journal of Cutaneous Pathology (1982), 9(5), 316-28

The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against ... [more ▼]

The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against collagen Types IV and V, and a proteoglycan. All these molecules are either basement membrane components or closely associated substances. In two types of EB simplex (subtype of the epidermolytic form) the splicing took place above the basement membrane, whereas the staining with all three antibodies remained localized to the floor of the blister. The herpetiform variant of EB simplex proved to be junctional, i.e. the separation occurred within the lamina lucida. One patient clinically classified as belonging to the junctional EB group, was found to have the epidermolytic form of the disease. In this case all antibodies were localized only on the floor of the blister. In the patients with the dermolytic form of EB, all the antibodies stained the roof of the blister. The immunofluorescence techniques are rapid and easy to perform and are therefore proposed as useful for routine clinical diagnosis. [less ▲]

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See detailThe immunohistology of glomerular antigens. IV. Laminin, a defined noncollagen basement membrane glycoprotein.
Scheinman, J. L.; Foidart, Jean-Michel ULg; Gehron-Robey, P. et al

in Clinical Immunology and Immunopathology (1980), 15(2), 175-89

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See detailThe immunohistology of glomerular antigens. V. The collagenous antigens of the glomerulus
Scheinman, J. L.; Foidart, Jean-Michel ULg; Michael, A. F.

in Laboratory Investigation : Journal of Technical Methods & Pathology (1980), 43(4), 373-81

Distinct antigenic loci are present within the human glomerulus as demonstrated by high resolution epifluorescent and phase-contrast microscopic techniques using affinity-purified antibody to ... [more ▼]

Distinct antigenic loci are present within the human glomerulus as demonstrated by high resolution epifluorescent and phase-contrast microscopic techniques using affinity-purified antibody to characterized collagens. Type IV mouse tumor procollagen and type V collagen are present within the mesangium and along the endothelial aspect of the glomerular basement membrane, demonstrating antigenic continuity between these two glomerular zones. In contrast, antiserum to type IV collagen from bovine lens capsule reacts with the full thickness of the glomerular basement membrane, as well as in the tubular basement membrane and Bowman's capsule. Types I procollagen and collagen are found within the epithelium; and types III collagen and procollagen are not detected within the glomerulus. The functional role of the multiple distinct "basement membrane" collagens in the glomerular capillary wall is unknown. A complex structure is suggested, especially in the mesangial-subendothelial continuity, and in the differential localization of two forms of type IV collagen within the glomerular basement membrane. [less ▲]

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See detailImmunolgic response to (pre)neoplastic cervical lesions associated with human papillomavirus
Delvenne, Philippe ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2005), 160(5-6), 287-93

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See detailImmunolocalization of laminin, fibronectin, and collagens I to V in the normal and mastotic breast
Gordenne, W.; Foidart, Jean-Michel ULg; Lapière, C. M.

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1982), 11(5), 549-54

Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the ... [more ▼]

Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the lactiferous ducts. In consists of a study of 35 samples which were taken from 13 women whose ages ranged from 40 to 75 and whose breasts were considered on clinical examination and on radiography as normal or abnormal. We have analyzed the distribution of anti-collagen types I, III, IV and V and anti-fibronectin and anti-laminin antibodies in certain sites in the breast which were chosen because of the diversity of forms they could take from one woman to another. In each case of the distribution of protein markers seemed to be regular without, as can be found in certain cases of scar or tumour formation, a change in the distribution of the matrix proteins. Therefore, as already suggested by certain histologists, benign abnormal breast conditions (fibrosis) should be considered as simple variations from the normal. This conclusion agrees with classical histological findings. [less ▲]

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See detailAn Immunologic investigation of canine eosinophilic bronchopneumopathy.
Clercx, Cécile ULg; Peeters, Dominique ULg; German, Alex et al

in Journal of Veterinary Internal Medicine (2002), 16

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See detailThe immunological 'self' of neuroendocrine protein families
Geenen, Vincent ULg

in Journal of Neuroimmunology (1994), 54

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See detailImmunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of Enterobacteria.
Falmagne, P.; Portetelle, Daniel ULg; Stalon, Victor

in Journal of Bacteriology (1985), 161

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See detailAn immunological cryo-ultrastructural study of a sequential appearance of proteins in placental binucleate cells in early pregnancy in the cow
Morgan, G.; Wooding, F. B. P.; Beckers, Jean-François ULg et al

in Journal of Reproduction and Fertility (1989), 86

Using the most sensitive immunocytochemical method available, on ultrathin frozen sections, the results in this paper demonstrate that bovine placental lactogen (bPL) is present in the earliest fetal ... [more ▼]

Using the most sensitive immunocytochemical method available, on ultrathin frozen sections, the results in this paper demonstrate that bovine placental lactogen (bPL) is present in the earliest fetal binucleate cells found at 21 days post coitum in the trophectoderm. A second protein, the SBU-3 antigen, which is absent in the early stages of pregnancy appears abruptly in the binucleate cell granules at 30 days post coitum coincident with the start of villus development. Subsequently, the granules contain both bPL and the SBU-3 antigen. This sequential production of unlike proteins indicates that the binucleate cell has different functions depending on the stage of pregnancy and has important roles to play both at implantation and in villus development. [less ▲]

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See detailImmunological detection of beta-galactosidase-expressing cells by flow cytometry
Vanderplasschen, Alain ULg; Goltz, M.; Hanon, E.

in Biochemica (1994), 3

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See detailImmunological discrimination between self and non self
Defaweux, Valérie ULg; Heinen, Ernst ULg

in Encyclopedia of Life Sciences (2010)

Detailed reference viewed: 43 (16 ULg)
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See detailImmunological effect of a synthetic growth hormone peptide on IGF-I and IGFBPs in growing pigs
Renaville, Robert ULg; Bertozzi, Carlo; Carelli, Claude et al

in 4th Intern. Symp. Insulin-like Growth Factors (1997)

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See detailAN IMMUNOLOGICAL METHOD TO COMBINE THE MEASUREMENT OF ACTIVE AND TOTAL HUMAN MYELOPEROXIDASE ON THE SAME SAMPLE FROM A COMPLEX MEDIUM
Franck, Thierry ULg; MINGUET, Grégory ULg; Mossay, Pierre et al

Conference (2013, September 12)

Active neutrophil myeloperoxidase (MPO) is a powerful producer of oxidant molecules in acute or chronic inflammation, and it is essential to measure its activity in biological samples. We combined two ... [more ▼]

Active neutrophil myeloperoxidase (MPO) is a powerful producer of oxidant molecules in acute or chronic inflammation, and it is essential to measure its activity in biological samples. We combined two immunological techniques, the SIEFED (specific immunologic extraction followed by enzymatic detection) and an ELISA, to measure the active and total contents of human MPO on the same sample and with the same calibration curve, to define an accurate ratio between the active and total enzyme. After the extraction of MPO from aqueous or biological samples by immobilized anti-MPO antibodies followed by a washing to eliminate unbound material, the active and the total contents of the enzyme were sequentially measured without interferences (patent: EP2017351-B1, 2010). Compared to a classical sandwich ELISA, there is one additional step corresponding to the in situ measurement of MPO activity, but this step does not affect the following measurement of the total MPO content. After validation, the combined technique was applied to a whole blood model of in vitro stimulation with phorbol-myristate-acetate (PMA), cytochalasin B/N-formyl-methionyl-leucyl-phenyl-alanine (CB/fMLP) or lipopolysaccharide/ tumor necrosis factor-alpha (LPS/TNF-α) (n=9). The active/total MPO ratio in whole blood reached 0.267±0.126 for non-stimulated condition and was significantly (p<0.05) higher for PMA (0.360±0.106), CB/fMLP (0.380±0.113) and LPS/TNF-α (0.432±0.124) stimulated conditions. These different ratios highlight the real oxidant potential of MPO, which depends on the stimulating conditions, witness of what could happen in pathological situations with diagnostic purpose. The combined SIEFED/ELISA method also appeared as a powerful tool to screen potential inhibitors that could interact directly with the enzyme, either on its active site or on another key position. [less ▲]

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See detailAn immunological method to combine the measurement of active and total myeloperoxidase on the same biological fluid, and its application in finding inhibitors which interact directly with the enzyme.
Franck, Thierry ULg; Minguet, G.; Delporte, C. et al

in Free radical research (2015)

Myeloperoxidase (MPO) is a pro-oxidant enzyme involved in inflammation, and the measurement of its activity in biological samples has emerged essential for laboratory and clinical investigations. We will ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme involved in inflammation, and the measurement of its activity in biological samples has emerged essential for laboratory and clinical investigations. We will describe a new method which combines the SIEFED (specific immunological extraction followed by enzymatic detection) and ELISA (ELISAcb) techniques to measure the active and total amounts of MPO on the same human sample and with the same calibration curve, as well as to define an accurate ratio between both the active and total forms of the enzyme. The SIEFED/ELISAcb method consists of the MPO extraction from aqueous or biological samples by immobilized anti-MPO antibodies coated onto microplate wells. After a washing step to eliminate unbound material, the activity of MPO is measured in situ by adding a reaction solution (SIEFED). Following aspiration of the reaction solution, a secondary anti-MPO antibody is added into the wells and the ELISAcb test is carried out in order to measure the total MPO content. To validate the combined method, a comparison was made with SIEFED and ELISA experiments performed separately on plasma samples isolated from human whole blood, after a neutrophil stimulation. The SIEFED/ELISAcb provides a suitable tool for the measurement of specific MPO activity in biological fluids and for the estimation of the inhibitory potential of a fluid. The method can also be used as a pharmacological tool to make the distinction between a catalytic inhibitor, which binds to MPO and inhibits its activity, and a steric inhibitor, which hinders the enzyme and prevents its immunodetection. [less ▲]

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See detailImmunological principles relevant for immune-mediated inflammatory diseases and pregnancy
Geenen, Vincent ULg

Scientific conference (2010, November 23)

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See detailImmunological reactivity of the Bovine Leukemia Virus glycoprotein gp51.
Portetelle, Daniel ULg; Bruck, Claudine; Cleuter, Yvette et al

in Straub O.C. (Ed.) Current Topics in Veterinary Medicine and Animal Science (1982)

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See detailImmunological Status of Competitive Football Players During the Training Season
Bury, Thierry ULg; Marechal, R.; Mahieu, P. et al

in International Journal of Sports Medicine (1998), 19(5), 364-8

We investigated possible immunological changes in 15 professional football players before, during and after the sports season. We studied the leucocyte count as well as different functions such as T ... [more ▼]

We investigated possible immunological changes in 15 professional football players before, during and after the sports season. We studied the leucocyte count as well as different functions such as T-lymphocyte proliferation, NK activity, chemotaxis and phagocytosis of neutrophils. Training and competitions did not produce any change in the total number of leucocytes but increased neutrophil counts and decreased T4 lymphocyte counts. We also observed a slight decrease of T-lymphocyte proliferation and a significant decrease of neutrophil functions. On the other hand, training and competitions did not induce significant changes in the number of NK cells nor in the total NK cytotoxic activity. The different change observed tended to normalize after the sports season. Our results suggest a predominant neutrophil function depression in football players during a training season which could partly explain the susceptibility of elite athletes to infections. [less ▲]

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See detailImmunological Techniques in Insect Biology (L.I. Gilbert & T.A. Miler, eds.)
Rentier, Bernard ULg

in Biochemical Systematics & Ecology (1989), 17(6), 501-502

Detailed reference viewed: 29 (0 ULg)