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See detailInsertion des jeunes en rupture et nouvelles cultures du lien - Recommandations et conclusions du colloque
Garcet, Serge ULg

Conference given outside the academic context (2006)

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See detailL'insertion des technologies dans le monde social
Mormont, Marc ULg; Bouquiaux, J. M.; Lauwers, L. et al

(2001)

La gestion du risque est partie intégrante et fondamentale des sociétés industrielles. Les questions environnementales fot émerger des incertitudes nouvelles de grande ampleur que les dispositifs ... [more ▼]

La gestion du risque est partie intégrante et fondamentale des sociétés industrielles. Les questions environnementales fot émerger des incertitudes nouvelles de grande ampleur que les dispositifs classiques ne peuvent encadrer ou limiter.Les nouvelles technologies apparaissent alors comme des risques non seulement d'accident mais de rupture du lien social et ceci rend nécessaire une discussion publique de leurs implications. [less ▲]

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See detailInsertion of domain III of Bacillus subtilis and Bacillus amyloliquefaciens PBP4a in the Bacillus licheniformis BlaP β-lactamase to study the binding to peptidoglycan and whole bacteria
Van Der Heiden, Edwige ULg; Hoebreck, Charline ULg; Freichels, Régine ULg et al

Poster (2013, October 03)

Domain III of Bacillus subtilis and B. amyloliquefaciens DD-endopeptidase PBP4a was introduced in the BlaP beta-lactamse of Bacillus licheniformis. Domain III of Bacillus licheniformis binds to ... [more ▼]

Domain III of Bacillus subtilis and B. amyloliquefaciens DD-endopeptidase PBP4a was introduced in the BlaP beta-lactamse of Bacillus licheniformis. Domain III of Bacillus licheniformis binds to peptidoglycan of Bacillus subtilis 168 and to itself whole cells. [less ▲]

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See detailInsertion Of Escherichia Coli Alpha-Haemolysin In Lipid Bilayers As A Non-Transmembrane Integral Protein: Prediction And Experiment
Soloaga, A.; Veiga, Mp.; Garcia-Segura, Lm. et al

in Molecular Microbiology (1999), 31(4), 1013-24

alpha-Haemolysin is an extracellular protein toxin (approximately 107 kDa) secreted by Escherichia coli that acts at the level of the plasma membranes of target eukaryotic cells. The nature of the toxin ... [more ▼]

alpha-Haemolysin is an extracellular protein toxin (approximately 107 kDa) secreted by Escherichia coli that acts at the level of the plasma membranes of target eukaryotic cells. The nature of the toxin interaction with the membrane is not known at present, although it has been established that receptor-mediated binding is not essential. In this work, we have studied the perturbation produced by purified alpha-haemolysin on pure phosphatidylcholine bilayers in the form of large unilamellar vesicles, under conditions in which the toxin has been shown to induce vesicle leakage. The bilayer systems containing bound protein have been examined by differential scanning calorimetry, fluorescence spectroscopy, differential solubilization by Triton X-114, and freeze-fracture electron microscopy. All the data concur in indicating that alpha-haemolysin, under conditions leading to cell lysis, becomes inserted in the target membrane in the way of intrinsic or integral proteins. In addition, the experimental results support the idea that inserted alpha-haemolysin occupies only one of the membrane phospholipid monolayers, i.e. it is not a transmembrane protein. The experimental data are complemented by structure prediction studies according to which as many as ten amphipathic alpha-helices, appropriate for protein-lipid interaction, but no hydrophobic transmembrane helices are predicted in alpha-haemolysin. These observations and predictions have important consequences for the mechanism of cell lysis by alpha-haemolysin; in particular, a non-transmembrane arrangement of the toxin in the target membrane is not compatible with the concept of alpha-haemolysin as a pore-forming toxin. [less ▲]

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See detailInsertion Of X-Ray Structures Of Proteins In Membranes
Basyn, F.; Spies, B.; Bouffioux, O. et al

in Journal of Molecular Graphics & Modelling (2003), 22(1), 11-21

Few structures of membrane proteins are known and their relationships with the membrane are unclear. In a previous report, 20 X-ray structures of transmembrane proteins were analyzed in silico for their ... [more ▼]

Few structures of membrane proteins are known and their relationships with the membrane are unclear. In a previous report, 20 X-ray structures of transmembrane proteins were analyzed in silico for their orientation in a 36A-thick membrane [J. Mol. Graph. Model. 20 (2001) 235]. In this paper, we use the same approach to analyze how the insertion of the X-ray structures varies with the bilayer thickness. The protein structures are kept constant and, at each membrane thickness, the protein is allowed to tilt and rotate in order to accommodate at their best. The conditions are said to be optimal when the energy of insertion is minimal. The results show that most helix bundles require thicker membranes than porin barrels. Moreover, in a few instances, the ideal membrane thickness is unrealistic with respect to natural membranes supporting that the X-ray structure requires adaptation to stabilize in membrane. For instance, the squalene cyclase could adapt by bending the side chains of its ring of lysine and arginine in order to increase the hydrophobic surface in contact with membranes. We analyzed the distribution of amino acids in the water, interface and acyl chain layers of the membrane and compared with the literature. [less ▲]

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See detailL’insertion professionnelle des comédiens
Brahy, Rachel ULg; Fraiture, Sébastien ULg; Megherbi, Salim ULg

Conference (2010, April 23)

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See detailL'insertion professionnelle des comédiens. Etude de cas à la sortie du conservatoire royal de Liège
Brahy, Rachel ULg; Fraiture, Sébastien ULg; Megherbi, Salim ULg et al

Book published by Les éditions de l'Université de Liège (2010)

Produit d’une étude financée par l’ASBL Théâtre et Publics et réalisée en 2008 au sein de l’Institut des Sciences Humaines et Sociales de l’Université de Liège, cet ouvrage traite de l’insertion ... [more ▼]

Produit d’une étude financée par l’ASBL Théâtre et Publics et réalisée en 2008 au sein de l’Institut des Sciences Humaines et Sociales de l’Université de Liège, cet ouvrage traite de l’insertion professionnelle des comédiens. L’analyse s’inscrit dans une démarche compréhensive et s’appuie principalement sur un matériau empirique de type qualitatif : entretiens semi-directifs de jeunes diplômés du Conservatoire de Liège, entretiens complémentaires d’acteurs clés de l’insertion dans ce secteur bien particulier, collecte ciblée de documents juridiques et institutionnels. L’intérêt de la démarche réside dans la prise en compte d’une multiplicité de variables ou dimensions d’ordre structurel (caractéristiques du marché du travail des comédiens, encadrement juridique de l’activité professionnelle, offre de formation, etc.) et d’ordre individuel (motivations, représentations subjectives, expériences, ressources, etc.), qui façonnent et orientent les trajectoires et stratégies d’insertion des comédiens, dès la sortie du Conservatoire. [less ▲]

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See detailInsertion professionnelle des diplômés de l’Université de Liège (promotion ’97) - Développement d’un outil d’évaluation
Italiano, Patrick ULg

Report (2000)

Ce rapport analyse les résultats d'une enquête de suivi, menée en 1999 auprès des étudiants de l'ULg de la promotion 1997. Il analyse leur insertion professionnelle, leurs difficultés et ressources dans ... [more ▼]

Ce rapport analyse les résultats d'une enquête de suivi, menée en 1999 auprès des étudiants de l'ULg de la promotion 1997. Il analyse leur insertion professionnelle, leurs difficultés et ressources dans la recherche d'un emploi, leur satisfaction quant aux études et l'emploi obtenu et la place du travail dans leur vie. [less ▲]

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See detailInsertion professionnelle des diplômés de l'Université de Liège (Promotion 2000)
Biquet, Véronique ULg; Lemaître, Sébastien

Report (2003)

Ce rapport analyse les résultats d'une enquête de suivi menée auprès des étudiants de l'ULg de la promotion 2000. Il analyse leur insertion professionnelle, leurs difficultés et ressources dans la ... [more ▼]

Ce rapport analyse les résultats d'une enquête de suivi menée auprès des étudiants de l'ULg de la promotion 2000. Il analyse leur insertion professionnelle, leurs difficultés et ressources dans la recherche d'un emploi, leur satisfaction quant aux études et l'emploi obtenu et la place du travail dans leur vie. [less ▲]

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See detailL'insertion professionnelle des diplômés de l'Université de Lubumbashi. Le cas des médecins
Rubbers, Benjamin ULg

in Akam, Noble; Ducasse, Roland (Eds.) Quelle université pour l'Afrique? (2002)

This article analyses the career of young doctors who graduated from the University of Lubumbashi

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See detailInsertion professionnelle et formation
Robert, Jocelyne ULg

in Le lien social. Actes du XIII° colloque de l'AISLF (1989)

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See detailL'insertion professionnelle peut-elle se cogérer ?
Pieters, Jérôme ULg; Jacquemain, Marc ULg

Conference (2004, November)

A partir des dispositifs de participation dans l'insertion professionnelle en Belgique francophone, la communication s'interroge sur la possibilité de pérenniser ce type d'expérience.

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See detailAn insertion-deletion polymorphism in the Interferon Regulatory Factor 5 (IRF5) gene confers risk of inflammatory bowel diseases
Dideberg, Vinciane ULg; Kristjansdottir, G.; Milani, L. et al

in Human Molecular Genetics (2007), 16(24), 3008-3016

The interferon regulatory factor 5 (IRF5) gene encodes a transcription factor that plays an important role in the innate as well as in the cell-mediated immune responses. The IRF5 gene has been shown to ... [more ▼]

The interferon regulatory factor 5 (IRF5) gene encodes a transcription factor that plays an important role in the innate as well as in the cell-mediated immune responses. The IRF5 gene has been shown to be associated with systemic lupus erythematosus and rheumatoid arthritis. We studied whether the IRF5 gene is also associated with inflammatory bowel diseases (IBD), Crohn disease (CD) and ulcerative colitis (UC). Twelve polymorphisms in the IRF5 gene were genotyped in a cohort of 1007 IBD patients (748 CD and 254 UC) and 241 controls from Wallonia, Belgium. The same polymorphisms were genotyped in a confirmatory cohort of 311 controls and 687 IBD patients (488 CD and 192 UC) from Leuven, Belgium. A strong signal of association [P=1.9x10(-5), odds ratio (OR) 1.81 (1.37-2.39)] with IBD was observed for a 5 bp indel (CGGGG) polymorphism in the promoter region of the IRF5 gene. The association was detectable also in CD patients (P=6.8x10(-4)) and was particularly strong among the UC patients [P=5.3x10(-8), OR=2.42 (1.76-3.34)]. The association of the CGGGG indel was confirmed in the second cohort [P=3.2x10(-5), OR=1.59 (1.28-1.98)]. The insertion of one CGGGG unit is predicted to create an additional binding site for the transcription factor SP1. Using an electrophoretic mobility shift assay, we show allele-specific differences in protein binding to this repetitive DNA-stretch, which suggest a potential function role for the CGGGG indel. [less ▲]

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See detailInsertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Godaux, Damien ULg; Emonds-alt, Barbara; Cardol, Pierre ULg et al

Poster (2011, September 18)

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases ... [more ▼]

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization. [less ▲]

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See detailInsertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Godaux, Damien ULg; Emonds-Alt, Barbara ULg; Cardol, Pierre ULg et al

Poster (2011, May 17)

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases ... [more ▼]

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization. To discover new genes involved in hydrogenases activity, we are also planning to screen the same insertional library for mutants with attenuated levels of hydrogen photoproduction, using sensitive chemochromic sensor films which turn in blue in presence of hydrogen. We are currently making the chemochromic sensor WO3 films by dip-coating which is on the brink of being useable. [less ▲]

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See detailLes insertions lyriques dans le théâtre médiéval
Henrard, Nadine ULg

in Grossel, Marie-Geneviève (Ed.) La chanson de trouvère — formes, registres, genres — (2012)

Pour mettre en évidence certains passages des mystères et structurer leur texte, les fatistes ont eu recours à des variations métriques. Il s’agit souvent de rompre la succession des octosyllabes à rimes ... [more ▼]

Pour mettre en évidence certains passages des mystères et structurer leur texte, les fatistes ont eu recours à des variations métriques. Il s’agit souvent de rompre la succession des octosyllabes à rimes plates par une diversification de l’agencement des rimes ou par l’utilisation d’autres types de vers. Mais les fatistes n’hésitent pas à couler les dialogues dans des structures beaucoup plus élaborées qui empruntent à des formes consacrées en poésie, comme celles du rondeau, ou plus sporadiquement, de la ballade. Il s’agira d’examiner comment ces morceaux lyriques s’insèrent dans le cours du drame, en s’interrogeant sur les lieux privilégiés où ils apparaissent, sur la manière dont les vers qui les composent se distribuent, et sur leur articulation éventuelle avec le paratexte didascalique (la rubrique permet-elle le repérage du morceau par une mention précise ? contient-elle des indications quant à une exécution musicale ? y a-t-il des verbes introducteurs spécifiques liés à ces énoncés ? ces passages entraînent-ils une mise en scène particulière ? etc). L'étude s'intéresse ainsi à la manière dont la lyrique est mise au service des effets dramatiques. Les exemples sont empruntés à des drames liturgiques bilingues, et des mystères en langue d’oïl et d'oc. [less ▲]

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See detailInside out transobturator vaginal tape for the treatment of female stress urinary incontinence: Interim results of a prospective study after a 1-year minimum followup
Waltregny, David ULg; Reul, Olivier ULg; Mathantu, Balombi et al

in Journal of Urology (The) (2006), 175(6), 2191-2195

Purpose: We analyzed the results of a prospective, observational trial designed to assess the safety and efficacy of the TVT-O procedure for female SUI. Materials and Methods: Preoperative and ... [more ▼]

Purpose: We analyzed the results of a prospective, observational trial designed to assess the safety and efficacy of the TVT-O procedure for female SUI. Materials and Methods: Preoperative and postoperative evaluations included physical examination, and urinary symptom and quality of life scale questionnaires. Results: Between March 2003 and December 2004, 253 patients with clinical and urodynamic diagnoses of SUI who fulfilled inclusion and exclusion criteria were enrolled in the trial and underwent the TVT-O procedure. No significant intraoperative complications were observed. One-year minimum followup was available on 99 of the initial 102 patients, of whom 16 had undergone concomitant pelvic organ prolapse surgical treatment. The SUI complete cure rate was 91%. No patient had vaginal or urethral erosion. Four patients required tape release or section. Frequency and urge symptoms improved after the operation (p < 0.001). The severity of obstructive symptoms slightly increased postoperatively in the group of patients who did not undergo associated pelvic organ prolapse treatment (p < 0.05), while maximum flow rates somewhat decreased (p < 0.001) and post-void residual urine volumes somewhat increased (p < 0.005). Most patients reported a significant decrease in incontinence severity and improvement in quality of life (p < 0.0001). Conclusions: The results of this study, which suggest that the TVT-O procedure is a safe and efficient surgical treatment for female SUI, warrant further comparative evaluation of this procedure with retropubic and outside in transobturator approaches in appropriately designed, prospective, randomized trials. [less ▲]

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