Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailGene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
Molina, Alfredo; Iyengar, Arati; Marins, Luis F. et al

in Biochimica et Biophysica Acta (2001), 1520(3), 195-202

We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also ... [more ▼]

We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish. [less ▲]

Detailed reference viewed: 49 (6 ULg)
Full Text
Peer Reviewed
See detailGene transcript quantitation by real-time RT-PCR in cells selected by immunohistochemistry-laser capture microdissection.
Lindeman, Neal; Waltregny, David ULg; Signoretti, Sabina et al

in Diagnostic Molecular Pathology : The American Journal of Surgical Pathology, Part B (2002), 11(4), 187-92

Studying tissue-based gene expression in different cell populations often requires immunohistochemistry-guided microdissection. However, mRNA degradation occurs during long staining procedures. We ... [more ▼]

Studying tissue-based gene expression in different cell populations often requires immunohistochemistry-guided microdissection. However, mRNA degradation occurs during long staining procedures. We combined a novel rapid immunoperoxidase technique with laser capture microdissection (LCM) and real-time quantitative RT-PCR to compare p27 mRNA expression in prostatic basal/secretory cells. Eight frozen prostate sections were immunostained with antibody 34betaE12 (high-molecular-weight keratin). Secretory and basal cells were separately collected by LCM. p27 transcripts from each cell group were quantitated by real-time RT-PCR, with GAPDH as standard. Immunostaining took 22 minutes, with RNA extraction from approximately 40 dissected cells from each compartment initiated within 40 minutes. Qualitative RT-PCR gave a product of the expected size from each sample. Quantitative RT-PCR gave basal/secretory p27/GAPDH ratios of 0.99-16.24 (mean 5.53 +/- 0.643). Immunostaining for keratin 34betaE12 can be done on frozen sections in approximately 20 minutes, and mRNA from pure cell populations can be quantitated by RT-PCR. We used this technique to show that p27 transcript levels are greater in basal than in secretory prostate cells, suggesting, when combined with prior studies, that regulation of p27 occurs at the protein level in normal cells. This technique may have wide applicability to studies of gene expression in distinct cell populations in heterogeneous tissues. [less ▲]

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailGene transfer in inner ear cells: a challenging race
Sacheli, Rosalie ULg; Delacroix, Laurence ULg; Van Den Ackerveken, Priscilla ULg et al

in Gene Therapy (2013), 20

Recent advances in human genomics led to the identification of numerous defective genes causing deafness, which represent novel putative therapeutic targets. Future gene-based treatment of deafness ... [more ▼]

Recent advances in human genomics led to the identification of numerous defective genes causing deafness, which represent novel putative therapeutic targets. Future gene-based treatment of deafness resulting from genetic or acquired sensorineural hearing loss may include strategies ranging from gene therapy to antisense delivery. For successful development of gene therapies, a minimal requirement involves the engineering of appropriate gene carrier systems. Transfer of exogenous genetic material into the mammalian inner ear using viral or non-viral vectors has been characterized over the last decade. The nature of inner ear cells targeted, as well as the transgene expression level and duration, are highly dependent on the vector type, the route of administration and the strength of the promoter driving expression. This review summarizes and discusses recent advances in inner ear gene-transfer technologies aimed at examining gene function or identifying new treatment for inner ear disorders. [less ▲]

Detailed reference viewed: 58 (26 ULg)
Full Text
Peer Reviewed
See detailGene-based replication strategy and meta-analysis in the context of epistasis: application for Alzheimer’s disease.
Gusareva, Elena ULg; van der Lee, Sven; Katsumata, Yuriko et al

Poster (2015, May 22)

Although the discovery of biological epistasis via statistical methods remains a big challenge, large-scale epistasis studies have become more and more popular nowadays. Considering the power of genome ... [more ▼]

Although the discovery of biological epistasis via statistical methods remains a big challenge, large-scale epistasis studies have become more and more popular nowadays. Considering the power of genome-wide association interaction analysis (GWAI) is conceptually lower when comparing to GWA using the same data, the genome-wide screenings for epistasis often ends with no statistically significant findings. Therefore, sound meta-analytic methodologies for combining evidence across independent gene-gene interaction studies, taking into account characteristics of individual studies, as well as protocols for good standard practice of meta-GWAI analysis are missing so far. The existing methodologies, whereas is suboptimal, may still be the first choice in meta-GWAI. In our study, the genome-wide significant interaction between two var¬¬iants of WWC1 and TLN2 genes were first discovered in males of EADI1 of Alzheimer’s disease (AD) case/control cohort. Adopting the gene-based replication approach recently advocated by Gusareva and Van Steen (Hum Genet 2014), we assessed association between all available variants from the pair of genes to test for pair-wise interactions in three independent AD replication cohorts (GERAD1, AD cohort from Rotterdam study - RS, and ADGC). Particularly, we used regression modeling with co-dominant coding of genetic markers (the one that was found to be the most powerful to identify epistasis and gives the least false positive results) and taking into account possible confounding factors (age and the first four PCs). We further meta-analyzed p-values for the interaction effects across all pairs of markers in males and females using Fisher summary test statistic. For the most interesting epistasis signal that occur in males of EADI1 cohort and two replication cohorts (RS and ADGC), we also conducted multi-locus genotype (MLG) association analysis (quantifies effects sizes of each of eight multilocus genotypes derived from the SNPs-pair versus the reference category - homozygous for the major alleles) and then met-analyzed results using the random effect or fixed effect models depending on presence of heterogeneity between studies. Adopting this approach we statistically replicated the interaction between WWC1 and TLN2 genes. Although, the biological validation of this finding is still ahead, we can already demonstrate effectiveness of the adopted meta-analysis pipeline to identify novel genetic factors potentially predisposing to AD. [less ▲]

Detailed reference viewed: 58 (2 ULg)
Full Text
See detailGene-based replication strategy and meta-analysis in the context of epistasis: application for Alzheimer’s disease.
Gusareva, Elena ULg; van der Lee, Sven; Katsumata, Yuriko et al

Speech/Talk (2015)

Although the discovery of biological epistasis via statistical methods remains a big challenge, large-scale epistasis studies have become more and more popular nowadays. Considering the power of genome ... [more ▼]

Although the discovery of biological epistasis via statistical methods remains a big challenge, large-scale epistasis studies have become more and more popular nowadays. Considering the power of genome-wide association interaction analysis (GWAI) is conceptually lower when comparing to GWA using the same data, the genome-wide screenings for epistasis often ends with no statistically significant findings. Therefore, sound meta-analytic methodologies for combining evidence across independent gene-gene interaction studies, taking into account characteristics of individual studies, as well as protocols for good standard practice of meta-GWAI analysis are missing so far. The existing methodologies, whereas is suboptimal, may still be the first choice in meta-GWAI. In our study, the genome-wide significant interaction between two var¬¬iants of WWC1 and TLN2 genes were first discovered in males of EADI1 of Alzheimer’s disease (AD) case/control cohort. Adopting the gene-based replication approach recently advocated by Gusareva and Van Steen (Hum Genet 2014), we assessed association between all available variants from the pair of genes to test for pair-wise interactions in three independent AD replication cohorts (GERAD1, AD cohort from Rotterdam study - RS, and ADGC). Particularly, we used regression modeling with co-dominant coding of genetic markers (the one that was found to be the most powerful to identify epistasis and gives the least false positive results) and taking into account possible confounding factors (age and the first four PCs). We further meta-analyzed p-values for the interaction effects across all pairs of markers in males and females using Fisher summary test statistic. For the most interesting epistasis signal that occur in males of EADI1 cohort and two replication cohorts (RS and ADGC), we also conducted multi-locus genotype (MLG) association analysis (quantifies effects sizes of each of eight multilocus genotypes derived from the SNPs-pair versus the reference category - homozygous for the major alleles) and then met-analyzed results using the random effect or fixed effect models depending on presence of heterogeneity between studies. Adopting this approach we statistically replicated the interaction between WWC1 and TLN2 genes. Although, the biological validation of this finding is still ahead, we can already demonstrate effectiveness of the adopted meta-analysis pipeline to identify novel genetic factors potentially predisposing to AD. [less ▲]

Detailed reference viewed: 30 (3 ULg)
See detailGénéalogie de la figure : Médiation esthétique et destination
Servais, Christine ULg

Book published by Presses universitaires du Septentrion (2001)

Detailed reference viewed: 38 (11 ULg)
See detailGénéalogie de la figure : Médiation esthétique et destination
Servais, Christine ULg

Doctoral thesis (2000)

Detailed reference viewed: 39 (7 ULg)
See detailGénéalogie de la peinture de paysage au XVIe siècle
Allart, Dominique ULg

in Toussaint, Jacques (Ed.) Autour de Henri Bles (2002)

Detailed reference viewed: 60 (10 ULg)
See detailLa généalogie de Tydée et de Diomède
Renaud, Jean-Michel ULg

in Auger, Danièle; Saïd, Suzanne (Eds.) Généalogies mythiques. Actes du VIIIe Colloque international du Centre de Recherches Mythologiques de l'Université de Paris-X (Chantilly, 14-16 septembre 1995) (1998, April)

Detailed reference viewed: 60 (3 ULg)
Full Text
Peer Reviewed
See detailGénéalogie éditoriale et génétique textuelle. Les premières éditions du Déserteur de Sébastien Mercier (1770-1772)
Droixhe, Daniel ULg

in Le livre et l'Estampe (2012), 58(178), 7-64

We establish the "stammbaum" organization of textual variations in editions whose printers are identified by their typographical material: Jay and Veuve Duchesne in Paris, Castaud in Lyon, Chambon in ... [more ▼]

We establish the "stammbaum" organization of textual variations in editions whose printers are identified by their typographical material: Jay and Veuve Duchesne in Paris, Castaud in Lyon, Chambon in Paris, Fantet in Besançon. We propose that some variations are due to the author, S. Mercier, himself and that Jay's edition of 1772, as well as an un-dated Brunet edition, announces the definitive 1786 edition. [less ▲]

Detailed reference viewed: 54 (0 ULg)
Full Text
See detailDe genegeerde kunst
Meesters, Gert ULg

Article for general public (2007)

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailA general and effective approach for the optimal design of fibers reinforced composite structures
Bruyneel, Michaël ULg

in Composites Science & Technology (2006), 66

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailA General and Natural Method to Define Circuit Relations Associated with Magnetic Vector Potential Formulations
Dular, Patrick ULg; Henrotte, François; Legros, Willy

in IEEE Transactions on Magnetics (1999), 35(3), 1630-1633

A procedure is proposed to deal with magnetic vector potential finite element magnetodynamic formulations and the definition of their associated circuit relations, involving voltages and currents ... [more ▼]

A procedure is proposed to deal with magnetic vector potential finite element magnetodynamic formulations and the definition of their associated circuit relations, involving voltages and currents, especially for massive inductors. It consists in solving two successive problems thanks to the use of edge elements, the first problem releasing the second one, which is especially useful for efficient nonlinear time stepping analyses [less ▲]

Detailed reference viewed: 17 (0 ULg)
Peer Reviewed
See detailGeneral Anti-Abuse Rules in Belgium income tax law
Bourgeois, Marc ULg

Conference (2014)

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailGeneral Anti-Avoidance Rules (GAARs) – A Key Element of Tax Systems in the Post-BEPS World, Belgian Report
Bourgeois, Marc ULg; Nollet, Aymeric ULg

in Lang, Michael (Ed.) General Anti-Avoidance Rules (GAARs) – A Key Element of Tax Systems in the Post-BEPS World (2016)

Detailed reference viewed: 28 (9 ULg)
Full Text
Peer Reviewed
See detailGeneral approach for homogenisation of multi-turn windings in 3D FE models
Gyselinck, Johan; V Sabariego, Ruth ULg; Dular, Patrick ULg

in Proceedings of the Sixth international conference on computation in electromagnetics (CEM2006) (2006)

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailA general approach to assess hydro-mechanical changes of natural clay barriers due to physico-chemical interactions with radwaste in deep disposal sites
Schmitz, Robrecht M; Schroeder, Christian ULg; Charlier, Robert ULg

in Physics & Chemistry of the Earth - Parts A/B/C (2007), 32(8-14), 922-928

Radwaste must be isolated from the biosphere for a long periods. To isolate waste, engineered clay barriers can be constructed. The functional life of such engineered barriers must be much longer (factor ... [more ▼]

Radwaste must be isolated from the biosphere for a long periods. To isolate waste, engineered clay barriers can be constructed. The functional life of such engineered barriers must be much longer (factor 100-1000) than that of more common engineered structures. Can the responsible engineer guarantee a functional life of several thousand years? Probably not, therefore waste like radwaste is stored at great depths, in a combination of geological and engineered barriers. During the storage period the waste itself (radiation) or derived products (oxidation of containers) can interact with the engineered and geological clay barriers. In,this contribution it is discussed how such interactions affect the hydro-mechanical properties of clay barriers. First some examples are given that show how properties of clays change at surface conditions if clays are exposed to typical waste products like different cations. Thereafter a method is proposed to classify the potential of hydro-mechanical modifications at three different scales: the particle level, the interlayer level and the tetrahedral-octahedral level. Next it is explained why the reactivity of clays at depth during long-term waste isolation is different (smaller) from the better known reactivity of clays at the surface. Instead of directing too much attention to such well known interactions, the focus for a safe deep disposal of radwaste should be directed to reactions that play a role at depth if, e.g. damage due to radiation occurs. Only if such reactions can be excluded, a safe storage of waste is possible. (C) 2006 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 40 (3 ULg)
Peer Reviewed
See detailA general approach to the harmonic balance finite element method for the modelling of 2D and 3D electromagnetic devices
Gyselinck, J.; Dular, Patrick ULg; Geuzaine, Christophe ULg et al

in Proceedings of the 11th COMPUMAG Conference on the Computation of Electromagnetic Fields (2001)

Detailed reference viewed: 27 (1 ULg)
Full Text
Peer Reviewed
See detailThe general approach to the patient with osteoarthritis : is a treatment algorithm feasible ?
Reginster, Jean-Yves ULg

in Osteoporosis International (2013), 24(1), 385

Detailed reference viewed: 15 (2 ULg)