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See detailExpression of histone deacetylase 8, a class I histone deacetylase, is restricted to cells showing smooth muscle differentiation in normal human tissues
Waltregny, David ULiege; de Leval, Laurence ULiege; Glenisson, Wendy et al

in American Journal of Pathology (2004), 165(2), 553-564

Histone deacetylases (HDACs) were originally identified as nuclear enzymes involved in gene transcription regulation. Until recently, it was thought that their activity was restricted within the nucleus ... [more ▼]

Histone deacetylases (HDACs) were originally identified as nuclear enzymes involved in gene transcription regulation. Until recently, it was thought that their activity was restricted within the nucleus, with histones as unique substrates. The demonstration that specific HDACs deacetylate nonhistone proteins, such as p53 and alpha-tubulin, broadened the field of activity of these enzymes. HDAC8, a class I HDAC, is considered to be ubiquitously expressed, as suggested by results of Northern blots performed on tissue RNA extracts, and transfection experiments using various cell lines have indicated that this enzyme may display a prominent nuclear localization. Using immunohistochemistry, we unexpectedly found that, in normal human tissues, HDAC8 is exclusively expressed by cells showing smooth muscle differentiation, including visceral and vascular smooth muscle cells, myoepithelial cells, and myofibroblasts, and is mainly detected in their cytosol. These findings were confirmed in vitro by nucleo-cytoplasmic fractionation and immunoblot experiments performed on human primary smooth muscle cells, and by the cytosolic detection of epitope-tagged HDAC8 overexpressed in fibroblasts. Immunocytochemistry strongly suggested a cytoskeleton-like distribution of the enzyme. Further double-immunofluorescence staining experiments coupled with confocal microscopy analysis showed that epitope-tagged HDAC8 overexpressed in murine fibroblasts formed cytoplasmic stress fiber-like structures that co-localized with the smooth muscle cytoskeleton protein smooth muscle alpha-actin. Our works represent the first demonstration of the restricted expression of a class I HDAC to a specific cell type and indicate that HDAC8, besides being a novel marker of smooth muscle differentiation, may play a role in the biology of these contractile cells. [less ▲]

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See detailExpression of Hoxa2 in cells entering chondrogenesis impairs overall cartilage development.
Massip, Laurent; Ectors, Fabien ULiege; Deprez, Pierre et al

in Differentiation : Research in Biological Diversity (2007), 75(3), 256-67

Vertebrate Hox genes act as developmental architects by patterning embryonic structures like axial skeletal elements, limbs, brainstem territories, or neural crest derivatives. While active during the ... [more ▼]

Vertebrate Hox genes act as developmental architects by patterning embryonic structures like axial skeletal elements, limbs, brainstem territories, or neural crest derivatives. While active during the patterning steps of development, these genes turn out to be down-regulated in specific differentiation programs like that leading to chondrogenesis. To investigate why chondrocyte differentiation is correlated to the silencing of a Hox gene, we generated transgenic mice allowing Cre-mediated conditional misexpression of Hoxa2 and induced this gene in Collagen 2 alpha 1-expressing cells committed to enter chondrogenesis. Persistent Hoxa2 expression in chondrogenic cells resulted in overall chondrodysplasia with delayed cartilage hypertrophy, mineralization, and ossification but without proliferation defects. The absence of skeletal patterning anomaly and the regular migration of precursor cells indicated that the condensation step of chondrogenesis was normal. In contrast, closer examination at the differentiation step showed severely impaired chondrocyte differentiation. In addition, this inhibition affected structures independently of their embryonic origin. In conclusion, for the first time here, by a cell-type specific misexpression, we precisely uncoupled the patterning function of Hoxa2 from its involvement in regulating differentiation programs per se and demonstrate that Hoxa2 displays an anti-chondrogenic activity that is distinct from its patterning function. [less ▲]

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See detailexpression of HSP70 mRNA and protein in rat hippocampus following heat shock
Krueger, Anne-Marie; Plumier, Jean-Christophe ULiege; Armstrong, John A. et al

Poster (1997)

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See detailThe expression of IL-1 β, iNOS and COX-2 genes by human chondrocytes is differentially regulated by reactive oxygen species
Mathy-Hartert, M; Deby, GP; Ayache, N et al

in Osteoarthritis and Cartilage (2001), 9SB

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See detailThe expression of IL-1bêta, iNOS and COX-2 genes by human chondrocytes in differentially regulated by reactive oxygen species
Henrotin, Yves ULiege; Mathy-Hartert, M; Deby, GP et al

in Clinical Rheumatology (2001), 20

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See detailThe expression of IL-1β, iNOS and COX-2 genes by human chondrocytes is differentially regulated by reactive oxygen species
Henrotin, Yves ULiege; Mathy, M; Deby, G et al

in Tissue Engineering (2001), 7

Detailed reference viewed: 19 (1 ULiège)
See detailExpression of insulin-related peptides in the human thymus
Geenen, Vincent ULiege; Lefebvre, Pierre ULiege

in International Diabetes Monitor (1997), 9

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See detailExpression of interleukin-4 receptor and ccl5 chemokine is not related to insect bite hypersensitivity in horses
Peeters, L. M.; Schroyen, Martine ULiege; Coussé, A. et al

in Journal of Equine Veterinary Science (2013), 33(8), 667-669

Insect bite hypersensitivity (IBH) in horses represents a type I or sometimes a type IV hypersensitivity to salivary antigens from numerous Culicoides spp and some other insects. Until now, there has been ... [more ▼]

Insect bite hypersensitivity (IBH) in horses represents a type I or sometimes a type IV hypersensitivity to salivary antigens from numerous Culicoides spp and some other insects. Until now, there has been no curative treatment available, but there are clear indications that the susceptibility to IBH is partly heritable. Identification of equine genes that are associated with susceptibility to IBH could lead to the development of a marker-assisted selection method. The aim of this study was to investigate the expression of functional candidate genes in relation to IBH. Based on literature, the interleukin-4 receptor (IL4R) and chemokine CCL5 (CCL5) genes were selected for examination by quantitative reverse transcription-PCR (qRT-PCR). Expression levels were determined in 16 horses: 8 IBH-positive horses, all showing clinical IBH symptoms at sampling; and 8 IBH-negative horses, stabled at the same location (case-control set up). Peripheral blood mononuclear cells (PBMC) were isolated from whole- blood samples, and expression levels of IL4R and CCL5 were measured. No differential expression was found for IL4R and CCL5 in PBMC between IBH-positive and IBH-negative horses (P=58 and P=63). Expression of CCL5 and IL4R in PBMC is not related to insect bite hypersensitivity in warmblood horses. Research toward a marker-assisted selection procedures could reduce IBH prevalence in horse populations by means of selection. © 2013 Elsevier Inc. [less ▲]

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See detailExpression Of Interleukin-6 Receptors And Interleukin-6 Messenger-Rna By Bovine Leukemia Virus-Induced Tumor-Cells
Droogmans, L.; Cludts, I.; Cleuter, Y. et al

in Cytokine (1994), 6(6),

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See detailExpression of interstitial collagenase (matrix metalloproteinase-1) is related to the activity of human endometriotic lesions
Kokorine, Isabelle; NISOLLE, Michelle ULiege; Donnez, Jacques et al

in Fertility and Sterility (1997), 68(2), 246-251

Objective: To determine whether interstitial collagenase (matrix metalloproteinase-1), known to play a pivotal role in the initiation of menstruation, contributes to the pathogenesis of endometriosis ... [more ▼]

Objective: To determine whether interstitial collagenase (matrix metalloproteinase-1), known to play a pivotal role in the initiation of menstruation, contributes to the pathogenesis of endometriosis. Design: Serial sections of peritoneal red and black endometriotic lesions, ovarian endometriotic cysts, and rectovaginal adenomyotic nodules were analyzed by in situ hybridization for the expression of matrix metalloproteinase-1 by silver staining for the integrity of the fibrillar extracellular matrix and by immunolabeling for the abundance of sex steroid receptors. Setting: Academic hospital and research laboratory. Patient(s): Premenopausal women undergoing laparoscopy for endometriosis. Intervention(s): Biopsy of endometriotic lesions, combined with endometrium whenever possible. Main Outcome Measure(s): Expression of matrix metalloproteinase-1 messenger RNA (mRNA). Result(s): Matrix metalloproteinase-1 mRNA was expressed focally in red peritoneal and ovarian endometriosis irrespective of the phase of the menstrual cycle but was not detectable in black peritoneal and rectovaginal lesions. Foci of matrix metalloproteinase-1 expression closely correlated with matrix breakdown and with the absence of P receptors in adjacent epithelial cells. Conclusion(s): Correlation of matrix metalloproteinase-1 expression with activity of endometriotic tissue suggests its involvement in tissue remodeling and bleeding, and possibly in the secondary shedding and reimplantation of endometriotic lesions. [less ▲]

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See detailExpression of intron-encoded maturase-like polypeptides in potato chloroplasts.
du Jardin, Patrick ULiege; Portetelle, Daniel ULiege; Harvengt, Luc et al

in Current Genetics (1994), 25(2), 158-163

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See detailExpression of Laminin by Human Fibroblasts, Ht1080 Fibrosarcoma Cells and Mcf-7 Breast Adenocarcinoma Cells. Lack of Regulation by the Cell Density and Extracellular Matrix
Munaut, Carine ULiege; Noël, Agnès ULiege; Sobel, M. et al

in Cell Biology International Reports (1991), 15(6), 499-509

We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 cells on various substrates (plastic, collagen type I, laminin). All cell types used adhered on the three ... [more ▼]

We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 cells on various substrates (plastic, collagen type I, laminin). All cell types used adhered on the three substrates with, however, a delayed attachment on laminin. On all substrates, cell grew as monolayer with the exception of MCF-7 cells that formed clusters on laminin. The epithelial MCF-7 cells as well as mesenchymal cells (fibroblasts and tumoral HT1080 cells) synthesized laminin and expressed mRNA coding for laminin B1 chain and for the 67 kD laminin binding protein. The levels of these mRNAs were not modulated by culture conditions which affect cell morphology nor by cell density. [less ▲]

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See detailExpression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
Sarafian, V.; Jadot, Michel ULiege; Foidart, Jean-Michel ULiege et al

in International Journal of Cancer = Journal International du Cancer (1998), 75(1), 105-11

Lysosomal-membrane-associated glycoproteins (Lamps) 1 and 2 are rarely found on the plasma membranes of normal cells. There is evidence suggesting an increase in their cell-surface expression in tumor ... [more ▼]

Lysosomal-membrane-associated glycoproteins (Lamps) 1 and 2 are rarely found on the plasma membranes of normal cells. There is evidence suggesting an increase in their cell-surface expression in tumor cells, with some data indicating that the adhesion of some cancer cells to the extracellular matrix is partly mediated by interactions between Lamps and E-selectin and between Lamps and galectins (endogenous-galactoside-binding lectins). The present study examined the expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in different human tumor cell lines. Indirect immunofluorescence staining revealed accumulation of Lamp molecules at the edges of A2058 human metastasizing melanoma cells suggesting that these glycoproteins could participate in cell adhesion. Flow cytometry showed the presence of cell-surface Lamps in A2058, HT1080 (human fibrosarcoma) and CaCo-2 (human colon-adenocarcinoma) cells. Treatment with 2 mM sodium butyrate for 24 and 48 hr resulted in a significant increase in Lamps surface expression. A strong binding of A2058 to recombinant galectin-3 was detected by FACS. The application of 2 and 5 mM butyrate for the same incubation period enhanced galectin-3 binding to Lamps-expressing cells. Our results support the idea that Lamps may be considered a new family of adhesive glycoproteins participating in the complex process of tumor invasion and metastasis. [less ▲]

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See detailExpression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta
Petignat, P.; Laurini, R.; Goffin, Frédéric ULiege et al

in International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society (2006), 16(4, JUL-AUG), 1679-1684

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs ... [more ▼]

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs and p53 in normal trophoblast and hydatidiform moles (HM). Paraffin sections of 45 specimens, including 14 complete hydatidiform moles (CM), 15 partial hydatidiform moles (PM), 8 atypical partial hydatidiform moles (aPM), and 8 controls were selected. Classification of HM was established on histologic criteria and supported by the DNA ploidy results. Tissue sections from each case were immunostained with monoclonal antibodies, cytokeratin-7, MMP-2, MMP-9, tissue inhibitors of metalloproteinases (TIMP)-1, and p53 wild type (p53wt) and mutant types (mutp53). Staining for cytokeratin-7 revealed a positive reaction in 93% of the samples. MMP-2 was mainly expressed in the syncytiotrophoblast of HM and found in 62% of aPM, 60% PM, and 93% CM. The mutp53 was mainly and focally expressed in syncytiotrophoblastic cells and was found in 63% of aPM, 80% PM, and 93% CM. Expression of MMP-2 and mutp53 was both significantly greater in HM vs control group (P < 0.05) and greater in CM vs PM and aPM (P < 0.05). No significant difference was observed for cytokeratin-7, MMP-9, TIMP-1, and p53wt between the HM subgroups and between HM and control group. MMP-2 and mutp53 are overexpressed in HM as compared with normal trophoblast and might participate in the invasive behavior of the HM. [less ▲]

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See detailExpression of members of the putative olfactory receptor gene family in mammalian germ cells.
Parmentier, M.; Libert, F.; Schurmans, Stéphane ULiege et al

in Nature (1992), 355

A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the ... [more ▼]

A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the unidentified receptors ('orphan receptors'), a human genomic clone (HGMP07) was characterized by the presence of its transcripts in the testis and by its belonging to a large subfamily of genes sharing extensive sequence similarities. Sequence comparison demonstrated that this gene subfamily is the human counterpart of the putative rat olfactory receptors cloned recently. Another 48 members of the family were cloned. Northern blotting further demonstrated the presence of olfactory receptor transcripts in germ cells. Our finding suggests that a common receptor gene family encodes olfactory receptors and sperm cell receptors that could be involved in chemotaxis during fertilization [less ▲]

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See detailExpression of membrane type 1 matrix metalloproteinase (MT1-MMP) in A2058 melanoma cells is associated with MMP-2 activation and increased tumor growth and vascularization
Sounni, Nor Eddine ULiege; Baramova, Eugénia; Munaut, Carine ULiege et al

in International Journal of Cancer = Journal International du Cancer (2002), 98(1), 23-28

Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase overexpressed in tumors, which plays a major role in the first step of pro-MMP-2 activation, leading to the generation of ... [more ▼]

Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase overexpressed in tumors, which plays a major role in the first step of pro-MMP-2 activation, leading to the generation of an intermediate 62 kDa species. The second step of MMP-2 activation that yields to the mature form is less understood and could involve an autocatalytic process and/or the activity of the plasminogen/plasmin system. Human melanoma A2058 cells, which express MMP-2 only in its pro-form, were used to determine the role of MT1-MMP during pericellular proteolysis and tumor progression. The induction of MT1-MMP overexpression by MT1-MMP cDNA transfection initiated the first step of MMP-2 activation. We provide evidence that a cooperation between the plasminogen/plasmin system and MT1-MMP endowed the cells with the ability to fully activate MMP-2 and with enhanced invasive properties in vitro. When injected subcutaneously in nude mice, MT1-MMP expressing clones induced rapid tumor growth and high tumor vascularization, while the control clones were poorly or not tumorigenic. Our data provide the first demonstration, in an experimental model, that MT1-MMP expression by tumor cells promotes tumor vascularization. [less ▲]

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See detailExpression of Mrna Encoding Alpha1e and Alpha1g Subunit in the Brain of a Rat Model of Absence Epilepsy
de Borman, B.; Lakaye, Bernard ULiege; Minet, Arlette ULiege et al

in Neuroreport (1999), 10(3), 569-74

Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding ... [more ▼]

Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding calcium channel alpha1E and alpha1G subunits was measured in the brain of genetic absence epilepsy rats from Strasbourg (GAERS). Using quantitative RT-PCR and in situ hybridization, no difference was found in alpha1G mRNA expression between GAERS and control animals, while a decreased expression of alpha1E was seen in the cerebellum and the brain stem of the GAERS. This phenomenon was not observed in young animals when the epileptic phenotype is not expressed. [less ▲]

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See detailExpression of MUC13 and MUC20 in relation to enterotoxigenic E. coli sensitivity in pigs
Schroyen, Martine ULiege; Stinckens, Anneleen; Verhelst, Roderick et al

Poster (2009)

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See detailExpression of osteopontin and its receptor the alphavbeta3 integrin in the 5TMM myeloma model
CAERS, Jo ULiege; De Raeve, Hendrik; Asosingh, Kewal et al

Poster (2004, September 17)

NA

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See detailExpression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH cells.
Rotondi, Sandra; Modarelli, Alessio; Oliva, Maria-Antonietta et al

in Molecular and cellular endocrinology (2016)

PURPOSE: To search for a possible role of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha), a molecular partner of the Aryl hydrocarbon receptor Interacting Protein (AIP), in somatotropinomas ... [more ▼]

PURPOSE: To search for a possible role of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha), a molecular partner of the Aryl hydrocarbon receptor Interacting Protein (AIP), in somatotropinomas. METHODS: Tumours from 51 acromegalic patients were characterized for PPARalpha and AIP expression by immunohistochemistry (IHC) and/or Real Time RT-PCR. Data were analysed according to tumour characteristics and pre-operative treatment with somatostatin analogues (SSA). The effects of fenofibrate were studied in GH3 cells in vitro. RESULTS: PPARalpha was expressed in most somatotropinomas. A modest relationship was found between PPARalpha and AIP expression, both being significantly higher in the presence of pre-operative SSA. However, only AIP expression was influenced by the response to treatment. Dual effects of fenofibrate were observed in GH3 cells, consisting of cell growth inhibition and an increase in GH secretion inhibited by octreotide. CONCLUSIONS: PPARalpha is a new player in somatotropinomas. Potential interactions between PPARalpha agonists and SSA may deserve further investigation. [less ▲]

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