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See detailInnovative Methodology for the Definition of Design Spaces of Chromatographic Methods
Rozet, Eric ULg; Debrus, B; Lebrun, Pierre ULg et al

Conference (2013, June 06)

As defined by ICH [1] and FDA, Quality by Design (QbD) stands for “a systematic approach to development that begins with predefined objectives and emphasizes product and process understanding and process ... [more ▼]

As defined by ICH [1] and FDA, Quality by Design (QbD) stands for “a systematic approach to development that begins with predefined objectives and emphasizes product and process understanding and process control, based on sound science and quality risk management”. A risk–based QbD–compliant approach is proposed for the robust development of analytical methods. This methodology based on Design of Experiments (DoE) to study the experimental domain models the retention times at the beginning, the apex and the end of each peak corresponding to the compounds of a mixture and uses the separation criterion (S) rather than the resolution (RS) as a Critical Quality Attribute. Stepwise multiple linear regressions are used to create the models. The estimated error is propagated from the modelled responses to the separation criterion (S) using Monte Carlo simulations in order to estimate the predictive distribution of the separation criterion (S) over the whole experimental domain. This allows finding ranges of operating conditions that will guarantee a satisfactory quality of the method in its future use. These ranges define the Design Space (DS) of the method. In chromatographic terms, the chromatograms processed at operating conditions within the DS will assuredly show high quality, with well separated peaks and short run time, for instance. This Design Space can thus be defined as the subspace, necessarily encompassed in the experimental domain (i.e. the knowledge space), within which the probability for the criterion to be higher than an advisedly selected threshold is higher than a minimum quality level. Precisely, the DS is defined as “the multidimensional combination and interaction of input variables (e.g., material attributes) and process parameters that have been demonstrated to provide assurance of quality” [1]. Therefore, this DS defines a region of operating conditions that provide prediction of assurance of quality rather than only quality as obtained with traditional mean response surface optimisation strategies. For instance, in the liquid chromatography there is a great difference in e.g. predicting a resolution (RS) higher than 1.5 vs. predicting that the probability for RS to be higher than 1.5 (i.e. P(RS> 1.5)) is high. The presentation of this global methodology will be illustrated for the robust optimisation and DS definition of several liquid chromatographic methods dedicated to the separation of different mixtures: pharmaceutical formulations, API and impurities/degradation products, plant extracts, separation of enantiomers, … [less ▲]

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See detailInnovative methodology to transfer conventional GC-MS heroin profiling to UHPLC-MS/MS
Debrus, Benjamin ULg; Broséus, Julian; Guillarme, Davy et al

in Analytical and Bioanalytical Chemistry (2011), 399(8), 2719-2730

Nowadays, in forensic laboratories, heroin profiling is frequently carried out by gas chromatography coupled with mass spectrometry (GC-MS). This analytical technique is well established, provides good ... [more ▼]

Nowadays, in forensic laboratories, heroin profiling is frequently carried out by gas chromatography coupled with mass spectrometry (GC-MS). This analytical technique is well established, provides good sensitivity and reproducibility, and allows the use of large databases. Despite those benefits, recently introduced analytical techniques, such as ultra-high-pressure liquid chromatography (UHPLC), could offer better chromatographic performance, which needs to be considered to increase the analysis throughput for heroin profiling. With the latter, chromatographic conditions were optimized through commercial modeling software and two atmospheric pressure ionization sources were evaluated. Data obtained from UHPLC–MS/MS were thus transferred, thanks to mathematical models to mimic GC-MS data. A calibration and a validation set of representative heroin samples were selected among the database to establish a transfer methodology and assess the models’ abilities to transfer using principal component analysis and hierarchical classification analysis. These abilities were evaluated by computing the frequency of successful classification of UHPLC–MS/MS data among GC-MS database. Seven mathematical models were tested to adjust UHPLC–MS/MS data to GC-MS data. A simplified mathematical model was finally selected and offered a frequency of successful transfer equal to 95%. [less ▲]

Detailed reference viewed: 138 (31 ULg)
See detailInnovative multilayered nanofibers for wound dressing application
Croisier, Florence ULg; Detrembleur, Christophe ULg; Jérôme, Christine ULg

Poster (2012, April 18)

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This ... [more ▼]

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This polysaccharide has shown a great potential for biomedical and pharmaceutical applications, on account of its remarkable compatibility with physiological medium. Besides, it is degraded in a physiological environment into non-toxic products, which make chitosan an outstanding candidate for short- to medium-term applications. In this respect, nanometric fibers are highly interesting as their assembly mimics the skin extracellular matrix structure. Such nanofibrous materials can be prepared by electrospinning (ESP). This technique uses a high voltage to create an electrically charged jet of polymer solution or melt which leads to fibers formation. Depending on the polymer characteristics (a.o. molecular weight, solution viscosity and conductivity) and processing conditions (electric potential, distance between syringe-capillary and collection plate, concentration, flow rate), polymer fibers ranging from nanometers to a few microns in diameter can be obtained and subsequently used as potential scaffolds, a.o. to form a temporary, artificial extracellular matrix. In the present study, electrospinning technique was combined with layer-by-layer deposition method (LBL) - a well-known method for surface coating, based on electrostatic interactions - in order to prepare multilayered chitosan-based nanofibers. The antibacterial properties of the obtained material were then assessed, and the presence of a multilayered deposit was confirmed by several techniques. (Future) possibilities for valorization: These multilayered chitosan-based nanofibers present great prospects for the preparation of new biomedical scaffolds - such as wound dressings that could improve skin regeneration. [less ▲]

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See detailInnovative Proteomics for the Discovery of Systemically Accessible Cancer Biomarkers Suitable for Imaging and Targeted Therapies
Turtoi, Andrei ULg; De Pauw, Edwin ULg; Castronovo, Vincenzo ULg

in American Journal of Pathology (2011), 178(1), 12-18

The discovery of biomarkers that are readily accessible through the circulating blood and are selectively overexpressed in pathological tissues has become a major research objective, particularly in the ... [more ▼]

The discovery of biomarkers that are readily accessible through the circulating blood and are selectively overexpressed in pathological tissues has become a major research objective, particularly in the field of oncology. Indisputably, this group of molecules has a high potential to serve as an innovative tool for effective imaging and targeted cancer therapy approaches. In this attractive therapeutic concept, specific cancer proteins are reached by intravenously administered ligands that are coupled to cytotoxic drugs. Such compounds are able to induce cancer destruction while sparing normal tissues. Owing to the performance of mass spectrometry technology, current high-throughput proteomic analysis allows for the identification of a high number of proteins that are differentially expressed in the cancerous tissues. However, such approaches provide no information regarding the effective accessibility of the biomarkers and, therefore, the possibility for these discovered proteins to be targeted. To bypass this major limitation, which clearly slows the discovery of such biomarkers, innovative methodological strategies have been developed to enrich the clinical specimens before the mass spectrometry analysis. The focus is laid on the group of proteins that are necessarily located either at the exterior face of the plasma membrane or in the extracellular matrix. The present review addresses the current technologies meant for the discovery and analysis of accessible antigens from clinically relevant samples. [less ▲]

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See detailInnovative smart sensors to enhance eco-efficiency of a continuous (steel) production line
Saint-Mard, Michel; Stoukatch, Serguei ULg; Schischke, Karsten et al

in Proc. Conf. of the AMA Association for Sensor Technology (2013, May 15)

The paper aims to demonstrate the benefits for a global approach that allows to define new research directions, new potential industrial markets, and increasing interest from potential users. Combining ... [more ▼]

The paper aims to demonstrate the benefits for a global approach that allows to define new research directions, new potential industrial markets, and increasing interest from potential users. Combining performance, economic and environmental criteria in a joint assessment approach for the target applications of sensor systems, facilitates significantly the communication between service provider and user. The environmental assessment approach for sensor systems will be available as a beta-version online tool tentatively from July 2013 onwards. Interested SMEs are invited to get involved as the LCA to go consortium will provide also a comprehensive mentoring for SMEs [less ▲]

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See detailAn innovative solution to improve the seismic robustness of reinforced concrete frames
Degée, Hervé ULg; Plumier, André ULg

in Proceedings of the 14ECEE Conference (2010)

Detailed reference viewed: 47 (4 ULg)
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See detailInnovative strategies for the control of apple scab (Venturia inaequalis [Cke.] Wint.) in organic apple production
Jamar, Laurent ULg

Doctoral thesis (2011)

The aim of this thesis, conducted over six growing seasons (2003-2008), is to define new measures for reducing the use of fungicides and in particular, copper fungicides, for the control of apple scab (V ... [more ▼]

The aim of this thesis, conducted over six growing seasons (2003-2008), is to define new measures for reducing the use of fungicides and in particular, copper fungicides, for the control of apple scab (V. inaequalis) in organic apple production. Special emphasis is put on primary scab infection control during spring season. An original approach is proposed for defining a specific spray timing involving spraying during the infection processes, especially before fungal penetration, determined by the RIMpro software warning system. This ‘during-infection’ spray strategy allows reducing from 30 to 50% the amount of fungicide usually used for effective apple scab control, on high scab-susceptible cultivars. The field study shows that six alternative products (e.g., potassium bicarbonate and lime sulphur), among 60 products tested, have the potential to reduce copper use. However, copper use, even with low doses, seems to be still necessary in presence of scab-susceptible cultivars. The results obtained in these experiments could not be attributed to the specific technical performances of the tunnel sprayer used, which however, offer valuable environmental benefits. On the basis of the present study, the organic management system seems a good farming approach for maintaining soil quality with regard to biological indicators. This work shows and draws attention to the fact that (i) planting cultivars with polygenic scab-resistance traits, (ii) increasing emphasis on sanitation practices aimed at reducing initial inoculum in autumn, and (iii) applying an accurate spray strategy in spring, as defined in the present study, are the three most promising approaches for substantial further reductions in protection products fully compliant with international organic crop production standards. [less ▲]

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See detailInnovative Surface Plasmon Resonance biosensing architectures
Desfours, Caroline; Piron, Pierre ULg; Habraken, Serge ULg et al

Conference (2012, December 11)

Surface Plasmons Resonance (SPR) architectures involving multi-wavelength interrogation are an attractive alternative for droplet biosensing. We present our results for in situ measurements of biological ... [more ▼]

Surface Plasmons Resonance (SPR) architectures involving multi-wavelength interrogation are an attractive alternative for droplet biosensing. We present our results for in situ measurements of biological molecules with a two-wavelength sensor and a SPR Coupler-Disperser spectroscopic sensor. [less ▲]

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See detailInnovative Surface Plasmon Resonance biosensing architectures
desfours, Caroline; Hastanin, Juriy ULg; Drucbert, Anne-Sophie et al

in International Conference on Fibre Optics and Photonics (2012, December)

Surface Plasmons Resonance (SPR) architectures involving multi-wavelength interrogation are an attractive alternative for droplet biosensing. We present our results for in situ measurements of biological ... [more ▼]

Surface Plasmons Resonance (SPR) architectures involving multi-wavelength interrogation are an attractive alternative for droplet biosensing. We present our results for in situ measurements of biological molecules with a two-wavelength sensor and a SPR Coupler- Disperser spectroscopic sensor. [less ▲]

Detailed reference viewed: 21 (5 ULg)
See detailInnovative utilization of the Lactoperoxidase System against apple’s postharvest diseases
Bafort, Françoise ULg; Jijakli, Haissam ULg; Perraudin, Jean-Paul

Conference (2012, September 21)

Detailed reference viewed: 34 (4 ULg)
See detailInnovatives techniques in implant dentistry
LAMBERT, France ULg

Scientific conference (2008)

Detailed reference viewed: 12 (0 ULg)
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See detailInnovativité et Modes de Coopération des Projets Inter-organisationnels Développés dans le Cadre d'une Politique Publique
Antoine, Mélanie ULg

in Bo, D.; Huron, D.; Spindler, J. (Eds.) Les pôles de compétitivité se décrètent-ils? Une comparaison européenne (2011)

Nous nous intéressons à la nature de l’innovation poursuivie par les projets inter-organisationnels initiés dans le cadre d'une politique publique, plus précisément à leur innovativité, ainsi qu'aux modes ... [more ▼]

Nous nous intéressons à la nature de l’innovation poursuivie par les projets inter-organisationnels initiés dans le cadre d'une politique publique, plus précisément à leur innovativité, ainsi qu'aux modes de coopération qui s’y développent. Une littérature émergente présente l'adéquation entre ces variables comme un facteur clé de succès des projets d'innovation multi-partenaires. Chaque type de projet appellerait un mode de pilotage approprié, les activités radicalement innovantes ne pouvant être gérées comme le développement d'innovations incrémentales. Dans cette étude, nous nous interrogeons sur la marge de manœuvre dont disposent les acteurs pour innover et organiser leurs actions lorsqu'ils répondent à l'initiative des autorités politiques. La recherche s'appuie sur deux études de cas de projets financés dans le cadre des Pôles de Compétitivité wallons. Alors qu'ils font face à des contraintes similaires, ces cas illustrent des configurations variées, tant en ce qui concerne la nature de l'innovation que les modes de coopération. L'analyse révèle en outre que les critères utilisés pour appréhender l'innovativité des projets, plutôt que de former un ensemble homogène, prennent des orientations différentes. Nous tentons alors d'identifier les principaux enjeux du pilotage des projets étudiés. [less ▲]

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See detailInnover ... ou périr !
Van Caillie, Didier ULg

Article for general public (2004)

This short chronicle recalls the strong link between innovation ability and performance and highlights the fact that failing firms are also often firm suffering from a lack of innovation.

Detailed reference viewed: 42 (4 ULg)
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See detailInnover à partir d’une ressource cognitive utopique : le cas des consultants en justice réparatrice
Dubois, Christophe ULg

in Bernard, Benoit (Ed.) Le management des organisations judiciaires (2009)

En publiant la circulaire ministérielle du 4 octobre 2000, Marc Verwilghen, alors ministre de la Justice, décide d’introduire le concept de justice réparatrice en milieu carcéral. Pour réaliser cet ... [more ▼]

En publiant la circulaire ministérielle du 4 octobre 2000, Marc Verwilghen, alors ministre de la Justice, décide d’introduire le concept de justice réparatrice en milieu carcéral. Pour réaliser cet ambitieux projet, il crée une nouvelle fonction – celle de consultant en justice réparatrice (CJR) – à qui il confie comme mission d’« orienter la culture de la détention vers une culture de la réparation », selon le texte de la circulaire. Ainsi, de 2000 à 2008, un CJR (consultant en justice réparatrice) a travaillé au sein de chaque établissement pénitentiaire belge. La présente contribution visera tout d’abord (2) à décrire le processus d’incarnation d’un dispositif d’action publique, c’est-à-dire le processus d’émergence de la fonction de CJR sur le terrain. Ensuite (3), nous tenterons de répertorier les pratiques qu’ont exercées, pendant près de huit ans, ces nouveaux acteurs du monde pénitentiaire. Nous analyserons (4) enfin quelles formes sociales concrètes les CJR ont conféré à leur métier. Pour ce faire, nous nous baserons sur un matériau empirique récolté au sein de quatre prisons pendant une période de trois ans. [less ▲]

Detailed reference viewed: 98 (18 ULg)