Migration-stimulating factor displays HEXXH-dependent catalytic activity important for promoting tumor cell migration

in International Journal of Cancer = Journal International du Cancer (2005), 116(3), 378-384

Like most extracellular matrix (ECM) components, fibronectin (Fn) is proteolyzed generating specific activities. Fibronectin proteinase (Fn-proteinase) represents such a cryptic activity located in the ... [more ▼]

Like most extracellular matrix (ECM) components, fibronectin (Fn) is proteolyzed generating specific activities. Fibronectin proteinase (Fn-proteinase) represents such a cryptic activity located in the gelatin-binding domain (GBD) of Fn and displays a zinc metalloproteinase activity. The migration-stimulating factor (MSF) is a truncated Fn isoform generated by alternative mRNA splicing and corresponds to the N-terminal part of Fn that comprises the GBD. We show that several human mammary epithelial cells express MSF and constitutively produce Fn-proteinase activity. Furthermore, recombinant M F produced by HEK-293 and MCF-7 cells possesses a constitutive Fn-proteinase activity. Mutating the putative zinc-binding motif, HEXXH, of the protein abolishes its activity thereby demonstrating its specificity. Using PCR, we showed that MSF is barely expressed in normal breast tissues, whereas its expression is significantly increased in tumors. Furthermore, an association between MSF expression and invasive capacity is observed in various breast adenocarcinoma cell lines. Indeed, when stably transfected in non-invasive MCF-7 cells, MSF promotes cell migration in a mechanism mostly dependent on its Fn-proteinase activity. In summary, our study shows that: (i) MSF displays constitutive Fn-proteinase activity; (ii) MSF expression is induced in human breast cancer; and (iff) MSF confers pro-migratory activity that depends mostly on its Fn-proteinase activity. These results suggest that MSF may be involved in tumor progression. (C) 2005 Wiley-Liss, Inc. [less ▲]

Migrations and family law

in Meeusen, Johan; Pertegas, Marta; Straetmans, Gert (Eds.) et al International Family Law for the European Union (2007)

migrations congolaises en Belgique et géographies religieuses

Conference (2012, April 13)

Les migrations de remontée des poissons dans la Meuse liégeoise et l’Ourthe. Bilan des connaissances et perspectives.

Conference (1998, October 21)

Les Migrations en Chine : Implications sur le plan

in Hommes & Migrations (1999), (n° 1220), 73-95

Migrations et capacités de franchissements d'obstacles chez la truite commune (Salmo trutta L.). Synthèse de 10 années de recherches par biotélémétrie dans les cours d'eau wallons.

Conference (2007, November 21)

Migrations et déplacements de populations dans un monde à +4°C. Scénarios d'évolution et options politiques.

in Etudes (2011), 414

« Migrations et Diversité Culturelle »

Scientific conference (2007, June 14)

Migrations et implantation du pentecôtisme congolais en Europe : processus d’alliance et intégration des églises diasporiques à la globalisation

Conference (2006)

Migrations et territoire religieux- Le cas des Eglises de réveil congolaises en Europe

Scientific conference (2010, November 16)