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See detailLes interactions médicamenteuses
Charlier, Corinne ULg

Conference (2003)

Detailed reference viewed: 8 (0 ULg)
See detailLes interactions médicamenteuses
Charlier, Corinne ULg

Conference (2002)

Detailed reference viewed: 6 (1 ULg)
See detailLes interactions médicamenteuses
Charlier, Corinne ULg

Conference (2002)

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See detailInteractions medicamenteuses: de la theorie a la pratique.
Scheen, André ULg

in Revue Médicale de Liège (2006), 61(5-6), 471-82

Pharmacotherapy becomes increasingly complex and therapeutic targets are more and more ambitious for both care and prevention. Therefore, polypharmacy is frequent, which exposes the patient to a high risk ... [more ▼]

Pharmacotherapy becomes increasingly complex and therapeutic targets are more and more ambitious for both care and prevention. Therefore, polypharmacy is frequent, which exposes the patient to a high risk of drug-drug interactions. These are often underestimated although they are responsible for many adverse events that may be serious and even fatal. In the present paper, we will first briefly recall the main cautions in the use of medications as well as the most common conditions favouring the occurrence of harmful drug interactions. Then, we will analyze the various mechanisms leading to drug interactions, emphasizing especially those involving cytochrome P450 enzymatic system and P-glycoprotein. Finally, we will illustrate these theoretical considerations with some examples of drug interactions, among the most dangerous and the most frequent in clinical practice. [less ▲]

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See detailInteractions Neuro-Immuno-Endocriniennes : Aspects moléculaires
Dantzer, Robert; Dardenne, Mireille; Haour, France et al

Book published by INSERM (1989)

Detailed reference viewed: 21 (0 ULg)
See detailInteractions neuroendocrino-immunitaires
Geenen, Vincent ULg; Legros, Jean-Jacques ULg; Franchimont, Paul

in Bazin, Hervé; Pastoret, Paul-Pierre; Govaerts, André (Eds.) Immunologie Animale (1990)

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See detailLes interactions neuroendocrino-immunitaires
Geenen, Vincent ULg

Conference given outside the academic context (1988)

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See detailInteractions neurotrophiques dans l'oreille interne en développement ?
Lefebvre, Philippe ULg; Weber, Thierry; Rigo, Jean-Michel et al

in Acta Otolaryngologica Belgica (1989), 43(5), 403-409

Detailed reference viewed: 16 (8 ULg)
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See detailInteractions of a potential plant elicitor mannolipid with plant model membranes
Polo Lozano, Damien ULg; Lins, Laurence ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act ... [more ▼]

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. The target of the elicitors is supposed to be the plant plasma membranes (PPM). The main mechanisms of interaction of many elicitors involve proteic receptors but lipid-based elicitors (LBE) may preferably interact with the lipidic fractions of PPM. However there is no detailed information at the molecular level on the PPM-LBE interactions. Our work is focused on a original synthetic LBE composed of a mannoside linked to a myristic acid. It has potential elicitor activities as shown by the assays on tobacco root cells. These activities could be related to its interaction with the lipidic phase of PPM. Since PPM are complex entities, the analyses of the PPM- molecule interactions are quite difficult. In this context, these interactions were carried out using biomimetic membranes of PPM such as Langmuir monolayers and multilayers. The effects of our molecule on these membranar systems were investigated by biophysical and in silico approaches. [less ▲]

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See detailThe interactions of apamin and tetraethylammonium are differentially affected by single mutations in the pore mouth of small conductance calcium-activated potassium (SK) channels
Dilly, Sébastien ULg; Philippart, Fabian ULg; Lamy, Cédric et al

in Biochemical Pharmacology (2013), 85

Valine residues in the pore region of SK2 (V366) and SK3 (V520) were replaced by either an alanine or a phenylalanine to evaluate the impact on the interactions with the allosteric blocker apamin. Unlike ... [more ▼]

Valine residues in the pore region of SK2 (V366) and SK3 (V520) were replaced by either an alanine or a phenylalanine to evaluate the impact on the interactions with the allosteric blocker apamin. Unlike TEA which showed high sensitivity to phenylalanine mutated channels, the binding affinity of apamin to the phenylalanine mutants was strongly reduced. In addition, currents from phenylalanine mutants were largely resistant to block by apamin. On the other hand, when the valine residue was replaced by an alanine residue, an increase of the binding affinity and the amount of block by apamin was observed for alanine mutated SK2 channels, but not for mutated SK3 channels. Interestingly, the VA mutation reduced the sensitivity to TEA. In silico data confirmed these experimental results. Therefore, such mutations in the pore region of SK channels show that the three-dimensional structure of the SK tetramers can be disorganized in the outer pore region leading to reduced interaction of apamin with its target. [less ▲]

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See detailInteractions of apamin with pore mutated SK3 channels
Dilly, Sébastien ULg; Lamy, Cédric; Poncin, Sylvie et al

Poster (2012, March 16)

In the present work, we have tested the impact of the replacement of valine residues in the pore region of SK3 (520) by either an alanine or a phenylalanine residue in terms of the interactions of apamin ... [more ▼]

In the present work, we have tested the impact of the replacement of valine residues in the pore region of SK3 (520) by either an alanine or a phenylalanine residue in terms of the interactions of apamin with these mutants in comparison with the corresponding native channels. Replacing valine residue at position 520 of the SK3 channel by a phenylalanine significantly increased the sensitivity of the channel to be blocked by tetraethylammonium (TEA) as previously reported. Indeed, an aromatic residue, such as a phenylalanine or a tyrosine, is frequently found in the pore region of several potassium channels more sensitive to TEA than SK channels. We measured the affinity (Kd) of apamin in saturation experiments and studied SK currents in transfected cells using patch clamp techniques. In parallel, molecular modelling techniques were used to examine the impact of these local modifications on the interaction of apamin with the corresponding channels. The presence of a phenylalanine in the pore region of potassium channels led to a higher sensitivity for TEA by creating more hydrophobic interactions as found by the docking procedure. In the in vitro binding experiments, the phenylalanine mutant (SK3VF) displayed a very low affinity for apamin. In patch clamp experiments, the SK current was only very partially blocked by apamin in the SK3VF mutant. Furthermore, apamin displayed an affinity and a blocking activity for the alanine mutant close to that for the corresponding native channels. In conclusion, the presence of a bulky and hydrophobic residue at a position near the pore mouth of SK3 channels has a negative impact on their interactions with apamin. [less ▲]

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See detailInteractions of apomyoglobin with membranes: Mechanisms and effects on heme uptake
Vernier, Gregory; Chenal, Alexandre; Vitrac, Heidi et al

in Protein Science : A Publication of the Protein Society (2007)

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See detailInteractions Of Ciprofloxacin With Dppc And Dppg: Fluorescence Anisotropy, Atr-Ftir And P-31 Nmr Spectroscopies And Conformational Analysis
Bensikaddour, H.; Snoussi, K.; Lins, Laurence ULg et al

in Biochimica et Biophysica Acta-Biomembranes (2008), 1778(11), 2535-43

The interactions between a drug and lipids may be critical for the pharmacological activity. We previously showed that the ability of a fluoroquinolone antibiotic, ciprofloxacin, to induce disorder and ... [more ▼]

The interactions between a drug and lipids may be critical for the pharmacological activity. We previously showed that the ability of a fluoroquinolone antibiotic, ciprofloxacin, to induce disorder and modify the orientation of the acyl chains is related to its propensity to be expelled from a monolayer upon compression [1]. Here, we compared the binding of ciprofloxacin on DPPC and DPPG liposomes (or mixtures of phospholipids [DOPC:DPPC], and [DOPC:DPPG]) using quasi-elastic light scattering and steady-state fluorescence anisotropy. We also investigated ciprofloxacin effects on the transition temperature (T(m)) of lipids and on the mobility of phosphate head groups using Attenuated Total Reflection Fourier Transform Infrared-Red Spectroscopy (ATR-FTIR) and (31)P Nuclear Magnetic Resonance (NMR) respectively. In the presence of ciprofloxacin we observed a dose-dependent increase of the size of the DPPG liposomes whereas no effect was evidenced for DPPC liposomes. The binding constants K(app) were in the order of 10(5) M(-1) and the affinity appeared dependent on the negative charge of liposomes: DPPG>DOPC:DPPG (1:1; M:M)>DPPC>DOPC:DPPC (1:1; M:M). As compared to the control samples, the chemical shift anisotropy (Deltasigma) values determined by (31)P NMR showed an increase of 5 and 9 ppm for DPPC:CIP (1:1; M:M) and DPPG:CIP (1:1; M:M) respectively. ATR-FTIR experiments showed that ciprofloxacin had no effect on the T(m) of DPPC but increased the order of the acyl chains both below and above this temperature. In contrast, with DPPG, ciprofloxacin induced a marked broadening effect on the transition with a decrease of the acyl chain order below its T(m) and an increase above this temperature. Altogether with the results from the conformational analysis, these data demonstrated that the interactions of ciprofloxacin with lipids depend markedly on the nature of their phosphate head groups and that ciprofloxacin interacts preferentially with anionic lipid compounds, like phosphatidylglycerol, present at a high content in these membranes. [less ▲]

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See detailInteractions of cryptolepine and neocryptolepine with unusual DNA structures
Guittat, Lionel; Alberti, Patrizia; Rosu, Frédéric ULg et al

in Biochimie (2003), 85(5), 535-547

Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a ... [more ▼]

Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Societe francaise de biochimie et biologic moleculaire. All rights reserved. [less ▲]

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See detailInteractions of human breast adenocarcinoma with the extracellular matrix - a new concept to study cancer invasion.
Foidart, Jean-Michel ULg; Verly, M.; Castronovo, Vincenzo ULg et al

in Evaluation du Risque de Cancer Mammaire. Chimiothérapie Première ? (1985)

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See detailInteractions of invasive cells with native and modified extracellular matrix in vitro.
Bracke, M.; Castronovo, Vincenzo ULg; De Bruyne, G. et al

in Advances in Experimental Medicine and Biology (1988), 233

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See detailInteractions of invasive cells with native and modified extracellular matrix in vitro.
Bracke, M.; Castronovo, Vincenzo ULg; De Bruyne, G. et al

in In Prodi, G.; Liotta, L. A.; Lollini, P. L. (Eds.) et al Cancer Metastasis : Biological and Biochemical Mechanisms and Clinical Aspects. (1988)

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See detailInteractions of iturinic antibiotics with plasma membrane. Contribution of biomimetic membranes.
Nasir, Mehmet Nail ULg; Besson, Françoise; Deleu, Magali ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2013), 17(3), 505-516

Iturinic antibiotics are produced by Bacillus subtilis strains and constitute a family including iturin A, mycosubtilin and bacillomycins D, F and Lc. They are cyclic lipopeptides having -amino fatty ... [more ▼]

Iturinic antibiotics are produced by Bacillus subtilis strains and constitute a family including iturin A, mycosubtilin and bacillomycins D, F and Lc. They are cyclic lipopeptides having -amino fatty acids linked up to a peptide constituted by seven -aminoacids with an invariable LDDLLDL chiral sequence. The first three -aminoacids containing the tyrosyl residue are the same for all members. They are well-known by their strong antifungal activities but they have also antibacterial and hemolytic properties. These biological properties are due to their amphiphilic nature allowing interactions with different membrane components. Sterols found in plasma membranes are the privileged interaction partners of these lipopeptides. Moreover, the tyrosyl residue of the iturinic antibiotics seems to play an important role during their fixation to the plasma membrane, the result of which is often the cellular lysis. Within plasma membranes, there are particular regions with high sterol content. These microdomains have a different composition compared to the rest of the membrane; they are enriched in certain lipids and proteins and they are involved in many key cellular processes. Their perturbation could then have an important impact on the cell. Due to their composition, these microdomains could constitute the preferential target of iturin antibiotics. This review aims to synthetize the works related to the biological activities of iturinic antibiotics and focusses especially to their understanding at the molecular level with a discussion on the key chemical groups of the iturin antibiotics and on the potentiality of microdomains to constitute a target for these molecules [less ▲]

Detailed reference viewed: 43 (12 ULg)