Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailInteraction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models
Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg; Perkins, Harold R

in European Journal of Biochemistry (1975), 57(2), 353-359

The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex ... [more ▼]

The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver-Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver-Burk plots can be obtained on the basis of a non-competitive type of inhibition. At present, a choice between the two models cannot be made. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction Between The N-Terminal Domain Of Gastric H,K-Atpase And The Spectrin Binding Domain Of Ankyrin Iii
Festy, F.; Robert, Jocelyne ULg; Brasseur, Robert ULg et al

in Journal of Biological Chemistry (2001), 276(11), 7721-6

We screened a cDNA bank of rabbit gastric fundic mucosa by two-hybrid assays looking for binding partners of the N-terminal domain of the rabbit gastric H,K-ATPase. We extracted five clones sharing more ... [more ▼]

We screened a cDNA bank of rabbit gastric fundic mucosa by two-hybrid assays looking for binding partners of the N-terminal domain of the rabbit gastric H,K-ATPase. We extracted five clones sharing more than 90% sequence identity. The longest clone codes for a protein sharing a high identity (96 and 96.8%, respectively) with a fragment of the membrane domain, from Arg-835 to Ser-873, plus the major part of the "spectrin binding domain" going from Glu-874 to Leu-1455 of human and mouse ankyrin III. We conclude that the membrane and spectrin binding domains of the rabbit ankyrin III are candidates for the binding partner of the N-terminal domain of the rabbit gastric H,K-ATPase. To validate the ankyrin-ATPase interaction and to test its specificity, we produced both domains in yeast and bacteria, coimmunoprecipitated them with an anti-ATPase antibody, and copurified them by affinity chromatography. The sequence of rabbit ankyrin III was not known, and this is the first report demonstrating that the ankyrin III and the H,K-ATPase interact with no intermediate. The interaction involves the N-terminal domain of the ATPase on one hand and the spectrin binding domain of the ankyrin on the other. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction between the vaccine of cervical cancer associated and natural killer (NK) cells
Renoux, Virginie ULg; Longton, Laurence; Dortu, Estelle ULg et al

Poster (2007, October 11)

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailInteraction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli
Bertsche, Ute; Kast, Thomas; Wolf, Benoît ULg et al

in Molecular Microbiology (2006), 61(3), 675-690

The murein (peptidoglycan) sacculus is an essential polymer embedded in the bacterial envelope. The Escherichia coli class B penicillin-binding protein (PBP) 3 is a murein transpeptidase and essential for ... [more ▼]

The murein (peptidoglycan) sacculus is an essential polymer embedded in the bacterial envelope. The Escherichia coli class B penicillin-binding protein (PBP) 3 is a murein transpeptidase and essential for cell division. In an affinity chromatography experiment, the bifunctional transglycosylase-transpeptidase murein synthase PBP1B was retained by PBP3-sepharose when a membrane fraction of E. coli was applied. The direct protein-protein interaction between purified PBP3 and PBP1B was characterized in vitro by surface plasmon resonance. The interaction was confirmed in vivo employing two different methods: by a bacterial two-hybrid system, and by cross-linking/co-immunoprecipitation. In the bacterial two-hybrid system, a truncated PBP3 comprising the N-terminal 56 amino acids interacted with PBP1B. Both synthases could be cross-linked in vivo in wild-type cells and in cells lacking FtsW or FtsN. PBP1B localized diffusely and in foci at the septation site and also at the side wall. Statistical analysis of the immunofluorescence signals revealed that the localization of PBP1B at the septation site depended on the physical presence of PBP3, but not on the activity of PBP3. These studies have demonstrated, for the first time, a direct interaction between a class B PBP (PBP3) and a class A PBP (PBP1B) in vitro and in vivo, indicating that different murein synthases might act in concert to enlarge the murein sacculus during cell division. [less ▲]

Detailed reference viewed: 38 (10 ULg)
Full Text
Peer Reviewed
See detailInteraction between virus like particles (VLP) and natural killer (NK) cells
Renoux, Virginie ULg; Dortu, Estelle ULg; Provenzano, Fabian et al

in Acta Clinica Belgica (2008), 2

Detailed reference viewed: 32 (14 ULg)
See detailInteraction carte hydrogéologique, forages d'eau et forages de géothermie
Debbaut, Vincent ULg

Conference (2013, May 16)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction entre familles récemment immigrées et services : une recherche-action pour le bien être des enfants
Manço, Altay; Born, Michel ULg; Petit, Sylvie

in Education Santé (2006), 209

Detailed reference viewed: 31 (6 ULg)
Full Text
Peer Reviewed
See detailInteraction entre modalité et subjectivité en néo-égyptien. Autour de la construction mri + iw circ. "souhaiter que"
Polis, Stéphane ULg

in Lingua Aegyptia [= LingAeg] (2009), 17

Typologically-oriented conceptions of modality have informed a number of recent publications in the field of Egyptian linguistics. These models have led to new insights regarding the linguistic structure ... [more ▼]

Typologically-oriented conceptions of modality have informed a number of recent publications in the field of Egyptian linguistics. These models have led to new insights regarding the linguistic structure of Egyptian. Especially promising is the interrelationship between modality and subjectivity, which has received considerable attention in general linguistic studies of modality but which has been hitherto neglected in the study of Egyptian. The aims of the present paper, which is intended as a preliminary exploration of this relationship, are [1] to study the semantic field of volition in Late Egyptian (whose primary exponents include: subjunctive; ib+suff. r + NP/INF.; Abi NP/(r +) INF.; wxA NP/INF.; mri NP/INF./iw circ.) and [2] to argue that in Late Egyptian one can distinguish between three main levels of syntactic integration for the complement clauses of verbs expressing the semantic notion of manipulation: complement clauses introduced by iw, subjunctive complement clauses, and infinitive complement clauses. These levels of syntactic integration correspond iconically to different levels of event integration; the degree of syntactic integration correlates with the strength of manipulation. [less ▲]

Detailed reference viewed: 107 (7 ULg)
Peer Reviewed
See detailThe interaction formulae for beam-columns: a new step of yet long story
Maquoi, René ULg; Boissonnade, N.; Muzeau, J. P. et al

in Proceedings 2001 of the SSRC Annual Technical Session and Meeting (2001)

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailAn interaction map for HTLV-1 Tax and PDZ-containing proteins.
Blibek, Karim ULg; Rambout, Xavier ULg; beaufays, Jérôme et al

Poster (2013, June 29)

Human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes for the Tax protein, which has a transforming capacity in vitro. Tax contains at its C-terminus a binding motif for PDZ domain-containing ... [more ▼]

Human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes for the Tax protein, which has a transforming capacity in vitro. Tax contains at its C-terminus a binding motif for PDZ domain-containing proteins (PSD95-DLG1-ZO1). It has been shown that the C-terminal motif of Tax is involved in Tax oncogenic capacity. Ten different PDZ domain-containing proteins have been reported to interact with Tax, but the specificity of Tax-human PDZome interactions has not been investigated. The objective of this study is to obtain a comprehensive interactome map for Tax and the human PDZome and to determine a global role of Tax-PDZ interactions in HTLV-1 biology. [less ▲]

Detailed reference viewed: 57 (16 ULg)
See detailInteraction matériau - vivant
Grandfils, Christian ULg

Learning material (2010)

Detailed reference viewed: 12 (0 ULg)
See detailInteraction médicamenteuse : de la théorie à l'ordonnance
Krzesinski, Jean-Marie ULg

Learning material (2006)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction network of antimicrobial peptides of Arabidopsis thaliana, based on hith-throughput yeast two-hybrid screening
Damon, Coralie ULg; Dmitrieva, Joelia Borisnova; Muhovski, Yordan et al

in Plant Physiology & Biochemistry (2012)

Detailed reference viewed: 25 (8 ULg)
Full Text
Peer Reviewed
See detailInteraction of 6,6-bis(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-1,2,4-benzotriazin-3-yl)-2,2:6,2-terpyridine (CyMe4-BTTP) with some trivalent ions such as lanthanide(III) ions and americium(III)
Lewis, Frank; Harwood, Laurance; Hudson, Michael J. et al

in Dalton Transactions (2010), 39

The new solvent extraction reagent 6,6’’-bis(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-1,2,4-benzotriazin-3-yl)-2,2’:6’,2’’-terpyridine (CyMe4-BTTP) has been synthesized in 4 steps from 2,2’:6’,2’’ ... [more ▼]

The new solvent extraction reagent 6,6’’-bis(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-1,2,4-benzotriazin-3-yl)-2,2’:6’,2’’-terpyridine (CyMe4-BTTP) has been synthesized in 4 steps from 2,2’:6’,2’’-terpyridine. Detailed NMR and mass spectrometry studies indicate that the ligand forms 1:2 complexes with lanthanide(III) perchlorates where the aliphatic rings are conformationally constrained whereas 1:1 complexes are formed with lanthanide(III) nitrates where the rings are conformationally mobile. An optimized structure of the 1:2 solution complex with Yb(III) was obtained from the relative magnitude of the induced paramagnetic shifts. X-ray crystallographic structures of the ligand and of its 1:1 complex with Y(III) were also obtained. The NMR and mass spectra of [Pd(CyMe4-BTTP)]n2n+ are consistent with a dinuclear double helical structure (n = 2). In the absence of a phase-modifier, CyMe4-BTTP in 1-octanol showed a maximum distribution coefficient of Am(III) of 0.039 (±20%) and a maximum separation factor of Am(III) over Eu(III) of 12.0 from nitric acid. From nitric acid, the metal(III) cations are extracted as the 1:1 complex. The generally low distribution coefficients observed compared with the BTBPs arise because the 1:1 complex of CyMe4-BTTP is considerably less hydrophobic than the 1:2 complexes formed by the BTBPs. In M(BTTP)3+ complexes, there is a competition between the nitrate ions and the ligand for the complexation of the metal. [less ▲]

Detailed reference viewed: 66 (24 ULg)
Full Text
Peer Reviewed
See detailInteraction of a slot-tone with a pipe
Billon, Alexis ULg; Valeau, Vincent; Sakout, Anas

in Journal of the Acoustical Society of America (2002, November), 111(5), 2373-2373

The self-sustained tone generated by a low Mach number free flow impinging on a slotted plate, usually referred to slot-tone, is experimentaly studied. In this work we focus on its coupling with the pipe ... [more ▼]

The self-sustained tone generated by a low Mach number free flow impinging on a slotted plate, usually referred to slot-tone, is experimentaly studied. In this work we focus on its coupling with the pipe from which the jet flows out. The jet nozzle and the beveled slot are aligned and have the same large aspect ratio. The tones generated have a single well defined frequency (around 1 kHz), a high amplitude (typically, 105 to 115 dB) and exhibit typical characteristics of self-sustained tones. Their frequencies are about the same order of magnitude than the natural frequency of the shear layer calculated from the linear stability theory. For some Reynolds numbers and some plate distances, a standing wave pattern is observed in the pipe. The outer and inner acoustic fields show high coherence and the slot-tone locks on a pipe eigenmode, which indicates an acoustic feedback. However, especially for lower Reynolds numbers, this coherence vanishes but the self-sustained tones are still present. It suggests that the hydrodynamic feedback becomes preponderant. [less ▲]

Detailed reference viewed: 12 (0 ULg)