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See detailIdentification Of Distinct Potyviruses In Mixedly-Infected Sweet-Potato By The Polymerase Chain-Reaction With Degenerate Primers
Colinet, D.; Kummert, J.; Lepoivre, Philippe ULg et al

in Phytopathology (1994), 84(1),

Detailed reference viewed: 18 (0 ULg)
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See detailIdentification of eae genes in rabbit attaching and effacing enteropathogenic Escherichia coli (EPEC)
Pohl, P.; Peeters, J. E.; Jacquemin, E. R. et al

in Infection and Immunity (1993), 61

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See detailIdentification of elastic orthotropic material parameters by inverse modeling using ESPI
Lecompte, D.; Sol, H.; Vantomme, J. et al

in Proceedings of the 2005 SEM Annual Conference & Exposition on Experimental and Applied Mechanics (2005)

Parameter identification methods, which integrate optimization techniques and numerical methods such as the finite element method (FEM), offer an alternative tool for material characterisation. The most ... [more ▼]

Parameter identification methods, which integrate optimization techniques and numerical methods such as the finite element method (FEM), offer an alternative tool for material characterisation. The most common approach is to determine the optimal estimates of the model parameters by minimizing a selected measure-of-fit between the responses of the system and the model. The possibility is studied of retrieving the four independent elastic engineering constants for an orthotropic medium, based on the measurement of a heterogeneous displacement field. In the present case a tensile test is performed on a perforated specimen. The responses of the system, i.e. the surface displacements are measured with an Electronic Speckle Pattern Interferometer. Strains are subsequently calculated, based on the measured displacement field. A finite element model of the perforated specimen is made. The difference between the experimental and numerical strains is minimized in a least squares sense by updating the values of the parameters. The obtained material (or model) parameters are very well in agreement with the traditionally determined ones. [less ▲]

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See detailIdentification of elastoplastic model in large deformation problems
Jeunechamps, Pierre-Paul ULg; Walmag, Jérôme ULg; Delhez, Eric ULg et al

in Cinquini, Carlo (Ed.) Proceedings of the 5th World Congress of Structural and Multidisciplinary Optimization WCSMO5 (2003, May)

This paper reports on preliminary results of parameter identification problems in finite element non-linear analyses such as metal forming simulations. Two approaches are compared. The first one is the ... [more ▼]

This paper reports on preliminary results of parameter identification problems in finite element non-linear analyses such as metal forming simulations. Two approaches are compared. The first one is the classic Levenberg-Marquardt algorithm. The second one is a trust-region algorithm based on a quadratic model. The two algorithms are compared on two cases, one of them being an actual experiment. [less ▲]

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See detailIdentification of elastoplastic model parameters in large deformation problems using gamma methods
Jeunechamps, Pierre-Paul ULg; Walmag, J.; Mathonet, V. et al

in Proceedings of the 5th World Congress on Structural and Multidisciplinary Optimization (2003)

This paper reports on preliminary results of parameter identification problems in finite element non-linear analyses such as metal forming simulations. Two approaches are compared. The first one is the ... [more ▼]

This paper reports on preliminary results of parameter identification problems in finite element non-linear analyses such as metal forming simulations. Two approaches are compared. The first one is the classic Levenberg-Marquardt algorithm. The second one is a trust-region algorithm based on a quadratic model. The two algorithms are compared on two cases, one of them being an actuel experiment. [less ▲]

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See detailIdentification of Electromechanical Coupling in Piezo-Structures
De Boe, Pascal; Golinval, Jean-Claude ULg

Conference given outside the academic context (2000)

An identification method based on a structural model updating procedure may be used to improve the knowledge of a piezoelectric tested structure and to determine the coupling coefficients of the ... [more ▼]

An identification method based on a structural model updating procedure may be used to improve the knowledge of a piezoelectric tested structure and to determine the coupling coefficients of the piezoelectric material. This procedure starts with the modal analysis of the open-loop instrumented structure. Let the target modes be a subset of the model modes; a selection of sensor (generally accelerometers) locations is then performed by determining the smaller subset such that the H2 modal norm is as close as possible to the modal norm of the original full set. In most cases, experimental testing with the selected sensor set will give acceptable information to identify target modes. These data, coupled with electrical sensing at the piezoelectric element level, will then be used to perform modal analysis of the piezo-structure. A pole-residue development of the open-loop piezo-structure shows that conventional algorithms may be used to estimate the mechanical modal parameters and the electromechanical coupling matrix. The second step of the procedure is to perform model updating itself. The initial finite element piezoelectric model will be improved stiffness corrections to the global stiffness matrix.. The corrections are split in their mechanical and electromechanical contributions. It is then possible to separate mechanical modelling errors from electromechanical coupling errors. The problem becomes a classical model updating problem which may be solved using well established techniques. This will result not only in a model behaving like the measures, but also in an improved knowledge of the structure behaviour without loosing physical insight. From a numerical point of view, it will be shown that ill-conditioning inherent to the presence of piezoelectric elements presents some difficulties at different steps of the model correction procedure. A clamped-free plate instrumented with piezo-laminates is used to illustrate the model updating approach. The selection of measurement points, using the modal norm criteria, is also presented. Experimental identification data will then be used as inputs for the model correction procedure and the behaviour of the updated model will be compared with the initial model dynamics. [less ▲]

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See detailIdentification of elemental sulphur (S8) in Boscia senegalensis (Pers.) Lam ex Poir. leaves.
Lognay, Georges ULg; Seck, D.; Marlier, M. et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(4),

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See detailIdentification of equid herpesvirus-5 in respiratory liquids: A retrospective study of 785 samples taken in 2006-2007
Fortier, Guillaume; Pronost, Stéphane; Miszczak, Fabien et al

in Veterinary Journal (2009), 182

During a case control study undertaken in 2006-2007, a screening and consensus polymerase chain reaction (PCR) was performed to evaluate the potential role of equid herpesviruses (EHV) in several ... [more ▼]

During a case control study undertaken in 2006-2007, a screening and consensus polymerase chain reaction (PCR) was performed to evaluate the potential role of equid herpesviruses (EHV) in several occurrences of respiratory disorders in 661 horses. Of 785 bronchoalveolar or tracheal lavage fluid samples submitted for analysis, 20 were positive for EHV-5 DNA by sequential analysis of the consensus PCR product. Nineteen of those samples were confirmed using a specific EHV-5 PCR. No particular changes in cytological profile could be associated with the detection of EHV-5 in contrast to suggestions in previous reports of natural or experimental respiratory viral infections in horses or ponies. This is the first description of EHV-5 isolation in equine respiratory fluids in Europe, but further investigations are needed to determine the potential pathogenic role of this gammaherpesvirus in the horse. [less ▲]

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See detailThe identification of experimental microscopic hafting traces on lithic tools
Rots, Veerle ULg

in Le Sécretariat du Congrès (Ed.) Sessions Générales et Posters / General Sessions and Posters. Acts of the XIVth UISPP Congress University of Liège, Belgium, 2 – 8 September 2001 (2003)

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See detailIdentification of factors important for the success of suicide gene therapy after a comparative study of Varicella zoster and Herpes simplex viral thymidine kinases efficacy on breast cancer cells
Grignet-Debrus, Christine ULg; Noël, Agnès ULg; Foidart, Jean-Michel ULg et al

in Cellular and Molecular Biology (2005), 51(1), 37-48

One of the most frequently studied therapeutic strategies in the field of suicide gene therapy is based on the expression by tumor cells of the Herpes simplex virus thymidine kinase gene (HSVtk) followed ... [more ▼]

One of the most frequently studied therapeutic strategies in the field of suicide gene therapy is based on the expression by tumor cells of the Herpes simplex virus thymidine kinase gene (HSVtk) followed by a ganciclovir (GCV) treatment. In order to investigate the potential of other enzyme/prodrug strategies, we studied in vitro and in vivo the ability of the Varicella zoster virus thymidine kinase gene (VZVtk) to act as a suicide gene and to kill non-transduced bystander cells, and compared this activity to that of its HSV counterpart. Four different antiviral compounds were tested as prodrugs. Our comparative study demonstrates the superiority of the HSVtk/GCV system among the different combinations tested and underlines the importance of both the tumor cell type and the prodrug in the success of a prodrug/suicide gene strategy. [less ▲]

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See detailIdentification of factors influencing the production of lipopeptides by Bacillus subtilis.
Jacques, Ph.; Hbid, Ch.; Razafindralambo, Hary ULg et al

Poster (1997, April)

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See detailIdentification of factors that maintain mammalian outer hair cells in adult organ of Corti explants
Malgrange, Brigitte ULg; Rigo, Jean-Michel; Coucke, Paul et al

in Hearing Research (2002), 170(1-2), 48-58

Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same ... [more ▼]

Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same culture conditions, only IHCs survive in explants from adult guinea pig organ of Corti while many of the OHCs are lost within the first 48 It. Hair cell Count,, show OHCs loss to be greater in the lower portion (i.e. middle turn) of the cochlea than Lit the apex. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) indicates that there is DNA damage in adult OHCs, within 8 h of explantation. Treatment of the adult organ of Corti explants with either actinomycin D (10(-7) M) or cycloheximide (10(-6) M) prevents most OHC losses . According to these results apoptosis may be the mechanism of OHC loss in adult organ of Corti explants, Stable membrane potentials recorded from the OHCs in both uncultured and actinomycin D-treated organ of Corti explants cultured for 72 h demonstrate the functional integrity of these hair cells. OHC losses in the adult guinea pig, organ of Corti cultures can also be prevented by treatment with several of the growth factors tested. i.e. acidic fibroblast growth factor (aFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), transforming growth factor-beta1 (TGF-beta1). and glial cell-derived neurotrophic factor (GDNF). The results of this study suggest that growth factor therapy may be applicable to the treatment of some hearing disorders. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailIdentification of feline panleukopenia virus proteins expressed in Purkinje cell nuclei of cats with cerebellar hypoplasia
Poncelet, Luc; Héraud, Céline; Springinsfeld, Marie et al

in The Veterinary Journal (2013), 196(3), 381-387

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See detailIdentification of Fragmentation Channels of Dinucleotides Using Deuterium Labeling.
Balbeur, D.; Dehareng, Dominique ULg; De Pauw, Edwin ULg

in Journal of the American Society for Mass Spectrometry (2010), 21(1), 23-33

The fragmentation of the totally deuterated dinucleotide dAT(-) in labile positions (heteroatom-bound hydrogens) was compared for different MS/MS methods: CID, IRMPD, and EID. These experiments allowed us ... [more ▼]

The fragmentation of the totally deuterated dinucleotide dAT(-) in labile positions (heteroatom-bound hydrogens) was compared for different MS/MS methods: CID, IRMPD, and EID. These experiments allowed us to affirm the coexistence of several fragmentation channels. They can be classified according to the involvement of nonlabile or labile protons in the fragmentation process. Moreover, double resonance experiments were performed in IRMPD and EID. They demonstrated the existence of consecutive fragmentation processes. The probability with which each channel is taken depends on the fragmentation technique used, i.e., the energy and the time scale of the method. The fragmentation channels that involve labile protons requiring peculiar three-dimensional structures are entropically unfavorable and enthalpically favorable. They are more observed in IRMPD and EID. The involvement of labile and, therefore, exchangeable protons in the fragmentation mechanism casts doubt on the use of tandem mass spectrometry to localize incorporated deuteriums in oligonucleotides. [less ▲]

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See detailIdentification of functional elements and regulatory circuits by Drosophila modENCODE.
Roy, Sushmita; Ernst, Jason; Kharchenko, Peter V. et al

in Science (2010), 330(6012), 1787-97

To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively ... [more ▼]

To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively mapping transcripts, histone modifications, chromosomal proteins, transcription factors, replication proteins and intermediates, and nucleosome properties across a developmental time course and in multiple cell lines. We have generated more than 700 data sets and discovered protein-coding, noncoding, RNA regulatory, replication, and chromatin elements, more than tripling the annotated portion of the Drosophila genome. Correlated activity patterns of these elements reveal a functional regulatory network, which predicts putative new functions for genes, reveals stage- and tissue-specific regulators, and enables gene-expression prediction. Our results provide a foundation for directed experimental and computational studies in Drosophila and related species and also a model for systematic data integration toward comprehensive genomic and functional annotation. [less ▲]

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See detailIdentification Of Functional-Sites On Bovine Leukemia-Virus Envelope Glycoproteins Using Structural And Immunological Data
Callebaut, I.; Portetelle, Daniel ULg; Burny, A. et al

in European Journal of Biochemistry (1994), 222(2),

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See detailIdentification of genes involved in the response of banana to crown rot disease
Lassois, Ludivine ULg; Frettinger, Patrick; de Lapeyre de Bellaire, Luc et al

in Molecular Plant-Microbe Interactions [=MPMI] (2011), 24(1), 143-153

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See detailIdentification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.
Qiu, Hui; Eifert, Julia; Wacheul, Ludivine et al

in Molecular & Cellular Biology (2008), 28(11), 3686-99

Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently ... [more ▼]

Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently localize to the Cajal body (in plant and animal cells) or the homologous nucleolar body (in budding yeast) for maturation and assembly into snoRNPs prior to accumulation in their primary functional site, the nucleolus. However, little is known about the trans-acting factors important for the intranuclear trafficking and nucleolar localization of snoRNAs. Here, we describe a large-scale genetic screen to identify proteins important for snoRNA transport in Saccharomyces cerevisiae. We performed fluorescence in situ hybridization analysis to visualize U3 snoRNA localization in a collection of temperature-sensitive yeast mutants. We have identified Nop4, Prp21, Tao3, Sec14, and Htl1 as proteins important for the proper localization of U3 snoRNA. Mutations in genes encoding these proteins lead to specific defects in the targeting or retention of the snoRNA to either the nucleolar body or the nucleolus. Additional characterization of the mutants revealed impairment in specific steps of U3 snoRNA processing, demonstrating that snoRNA maturation and trafficking are linked processes. [less ▲]

Detailed reference viewed: 18 (0 ULg)