Immunohistochemical localization of basement membrane components and interstitial collagen types in preovulatory rat ovarian follicles.
; ; Foidart, Jean-Michel et al
in Collagen and Related Research (1984), 4(4), 279-87
Cryosections of ovaries from rats treated with pregnant mare serum gonadotropin (PMSG) were immunostained with antibodies against collagen types, I, III, IV and V, laminin and heparan sulphate ... [more ▼]
Cryosections of ovaries from rats treated with pregnant mare serum gonadotropin (PMSG) were immunostained with antibodies against collagen types, I, III, IV and V, laminin and heparan sulphate proteoglycan using the peroxidase-antiperoxidase technique. A uniform belt-like staining was observed between the granulosa and theca interna layers with antibodies against type IV collagen, laminin and heparan sulphate proteoglycan. Interstitial collagens type I and III stained the connective tissue in the theca externa layer but gave only faint staining in the area between the granulosa and theca interna zones. If the PMSG-treated rats were injected with human chorionic gonadotropin the number of follicles that showed a discontinuous, ragged or disrupted staining reaction in the area between the granulosa and theca interna layers was clearly increased. Surprisingly in such cases also a punctate staining reaction was seen in the basal granulosa cells. When the rats were injected with radioactive human chorionic gonadotropin, most of the ovarian follicles were labeled within 2 hours. These follicles still showed a continuous staining reaction with antibodies against basement membrane components at the border of the granulosa and theca interna cell layers. These results suggest that there is a basement membrane-like structure in Graafian follicles between the granulosa cells and theca interna layer, and that this basement membrane is disrupted after human chorionic gonadotropin stimulation. [less ▲]Detailed reference viewed: 5 (0 ULg)
Immunohistochemical localization of laminin, type IV and type V collagen in basal cell carcinoma.
; Pierard, Gérald ; Foidart, Jean-Michel et al
in British Journal of Dermatology (1983), 108(2), 163-70
Laminin, type IV and type V collagen were localized in twenty-five basal cell carcinomas (BCCs) by immunofluorescence. All basal cell carcinomas were separated from the connective tissue by a continuous ... [more ▼]
Laminin, type IV and type V collagen were localized in twenty-five basal cell carcinomas (BCCs) by immunofluorescence. All basal cell carcinomas were separated from the connective tissue by a continuous basement membrane (BM) which always contained the three antigenic determinants. Nodular and cystic BCCs, but not pagetoid BCCs, contain linear and globular deposits of these BM components. The linear deposits probably represent BM debris that remains undigested and the globular deposits correspond to residual cytoplasmic fragments occurring during apoptosis. These results suggest that BM components are synthesized by epithelial cells and are secreted to polymerize at the dermo-epidermal junction. [less ▲]Detailed reference viewed: 5 (0 ULg)
Immunohistochemical patterns in the interfollicular Caucasian scalps. Influence of age-, gender- and alopecia.
Franchimont, Claudine ; ; et al
in BioMed Research International (2013), 2013(769489),Detailed reference viewed: 15 (3 ULg)
An immunohistochemical study of canine nasal aspergillosis
Peeters, Dominique ; ; Clercx, Cécile
in Journal of Comparative Pathology (2005), 132(4), 283-288
In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most ... [more ▼]
In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most consistent histopathological finding was a severe, predominantly lymphoplasmacytic, inflammatory infiltration of the lamina propria. Fungal hyphae were not observed to invade the mucosa but were found at the mucosal surface and within material collected from the nasal cavity. The main immunohistochemical findings were (1) a predominance of IgG(+) plasma cells over IgA(+) and IgM(+) plasma cells, (2) significant numbers of macrophages and dendritic cells expressing MHC class 11 molecules, (3) macrophages and neutrophils expressing L1 antigen and (4) a mixture of CD4(+) and CD8(+) T cells. These findings are consistent with a dominant Th1-regulated cell-mediated immune response. The nature of the inflammatory infiltrate and the lack of invasiveness of the mucosa by the fungus, together with the clinical course of the disease and the apparent immunocompetence of the affected dogs, suggest that canine nasal aspergillosis resembles the chronic erosive non-invasive fungal sinusitis described in human patients. © 2004 Elsevier Ltd. All rights reserved. [less ▲]Detailed reference viewed: 19 (1 ULg)
An immunohistochemical study of canine nasal aspergillosis.
Peeters, Dominique ; ; et al
in Proceedings of The 14th Annual Congress of the ECVIM-CA (2004)Detailed reference viewed: 8 (0 ULg)
An immunohistochemical study of eosinophilic bronchopneumopathy in dogs.
Peeters, Dominique ; ; Clercx, Cécile
in Proceedings of the 14th Annual Congress of the ECVIM-CA (2004)Detailed reference viewed: 16 (1 ULg)
An Immunohistochemical Study of the Inflammatory Infiltrate Associated with Nasal Carcinoma in Dogs and Cats.
Vanherberghen, Morgane ; ; et al
in Journal of Comparative Pathology (2009), 141(1)Detailed reference viewed: 14 (5 ULg)
Immunohistochemical study of the proliferation index, oestrogen receptors and progesterone receptors A and B in leiomyomata and normal myometrium during the menstrual cycle and under gonadotrophin-releasing hormone agonist therapy
NISOLLE, Michelle ; ; et al
in Human Reproduction (1999), 14(11), 2844-2850
The cell proliferation-associated antigen Ki 67 and the immunohistochemical content of oestrogen receptors (ER), progesterone receptors AB (PRAB) and progesterone receptors B (PRB) were evaluated in ... [more ▼]
The cell proliferation-associated antigen Ki 67 and the immunohistochemical content of oestrogen receptors (ER), progesterone receptors AB (PRAB) and progesterone receptors B (PRB) were evaluated in leiomyomata and adjacent myometrium during the menstrual cycle and in leiomyomata under gonadotrophin-releasing hormone agonist (GnRHa) therapy. The proliferative status of muscular cells was measured by evaluating the percentage of nuclei staining positive for Ki 67 (proliferation index). Quantitative analysis (QH-score) was carried out using advanced stereographic computer technology to investigate ER, PRAB and PRB. Leiomyoma and myometrial biopsies were taken from 30 patients undergoing hysterectomy or myomectomy because of symptomatic leiomyomata (subgroup I). Leiomyoma biopsies were taken from 10 patients suffering from symptomatic submucosal leiomyomata, after 2 month GnRH therapy (subgroup II). During the secretory phase, the proliferation index (Ki 67) was found to be higher in leiomyomata than in myometrium, but the difference was not significant. Oestrogen receptor content was significantly higher in leiomyomata than in myometrium only during the proliferative phase of the cycle. PRAB and PRB content were found to be higher in leiomyomata than in adjacent myometrium with a statistically significant dominance of PRAB over PRB. Under GnRHa therapy, a dramatic decrease was observed in PRAB and B content as well as Ki 67 but ER content remained comparable with the results obtained during the menstrual cycle. The results suggest that leiomyomata may be under the influence of progesterone which may play a major role in their growth. [less ▲]Detailed reference viewed: 16 (2 ULg)
Immunohistochemical study of the rectus sheath and transversalis facia in adult groin hernias
PANS, Alain ; Pierard, Gérald ; Albert, Adelin
in Hernia : the Journal of Hernias & Abdominal Wall Surgery (1999), 3Detailed reference viewed: 4 (0 ULg)
Immunohistochemical study on cultured FDC-C enriched lymphoid cell populations.
; ; Heinen, Ernst et al
in Advances in Experimental Medicine and Biology (1988), 237Detailed reference viewed: 17 (3 ULg)
Immunohistochemical sweat gland profiles.
; Pierard, Gérald ; Delvenne, Philippe et al
in Journal of Cosmetic Dermatology (2013), 12
Abstract BACKGROUND: Human sweat glands are heterogeneous in their structures and functions. Accordingly, eccrine, apocrine, and apoeccrine glands are distinguished. AIMS: Some immunohistochemical markers ... [more ▼]
Abstract BACKGROUND: Human sweat glands are heterogeneous in their structures and functions. Accordingly, eccrine, apocrine, and apoeccrine glands are distinguished. AIMS: Some immunohistochemical markers are expected to distinguish the sweat gland types in their secretory and excretory parts. METHODS: This study used two sets of antibodies. The first panel was composed of antibodies directed to well-defined sweat gland structures. The molecular targets included the low-molecular-weight cytokeratins CAM 5.2, the S100-B protein, the epithelial membrane antigen (EMA), the carcinoembryonic antigen (CEA), and the lectin Ulex europaeus agglutinin-1 (UEA-1). A second exploratory panel of antibodies targeted syndecan-1 (CD138), NKI-C3 (CD63), and CD68. They were used to disclose some undescribed antigen expressions in human sweat glands. RESULTS: The first set of antibodies confirmed previous findings. The immunoreactivities of the three sweat gland types were similar in the excretory ducts. By contrast, they were distinguished in the deeper coiled secretory portions of the glands. CONCLUSION: Clues supporting their distinction and probably their functional activity were obtained by immunohistochemistry using the S100-B protein, CEA and CD63 antibodies. The immunoreactivity to the S100-B protein, CEA and CD63 possibly help identifying apoeccrine sweat glands or a peculiar functional activity of eccrine sweat glands. [less ▲]Detailed reference viewed: 23 (8 ULg)
Immunohistochemical transforming growth factor Beta1 and its activation, storage and signalling pathways in canine idiopathic pulmonary fibrosis
Krafft, Emilie ; ; et al
in Proceedings of the 23th ECVIM Meeting (2013, September)Detailed reference viewed: 12 (2 ULg)
Immunohistological Demonstration Of Virus And Tumor Associated Antigens In Tissues In Experimental And Spontaneous Bovine Leukemia-Virus (Blv) Infection
; ; et al
in Veterinary Immunology and Immunopathology (1989), 22(3),Detailed reference viewed: 8 (0 ULg)
Immunohistological localization of three basement membrane components in various forms of epidermolysis bullosa.
; ; Foidart, Jean-Michel et al
in Journal of Cutaneous Pathology (1982), 9(5), 316-28
The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against ... [more ▼]
The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against collagen Types IV and V, and a proteoglycan. All these molecules are either basement membrane components or closely associated substances. In two types of EB simplex (subtype of the epidermolytic form) the splicing took place above the basement membrane, whereas the staining with all three antibodies remained localized to the floor of the blister. The herpetiform variant of EB simplex proved to be junctional, i.e. the separation occurred within the lamina lucida. One patient clinically classified as belonging to the junctional EB group, was found to have the epidermolytic form of the disease. In this case all antibodies were localized only on the floor of the blister. In the patients with the dermolytic form of EB, all the antibodies stained the roof of the blister. The immunofluorescence techniques are rapid and easy to perform and are therefore proposed as useful for routine clinical diagnosis. [less ▲]Detailed reference viewed: 11 (0 ULg)
The immunohistology of glomerular antigens. IV. Laminin, a defined noncollagen basement membrane glycoprotein.
; Foidart, Jean-Michel ; et al
in Clinical Immunology and Immunopathology (1980), 15(2), 175-89Detailed reference viewed: 5 (0 ULg)
The immunohistology of glomerular antigens. V. The collagenous antigens of the glomerulus
; Foidart, Jean-Michel ;
in Laboratory Investigation : Journal of Technical Methods & Pathology (1980), 43(4), 373-81
Distinct antigenic loci are present within the human glomerulus as demonstrated by high resolution epifluorescent and phase-contrast microscopic techniques using affinity-purified antibody to ... [more ▼]
Distinct antigenic loci are present within the human glomerulus as demonstrated by high resolution epifluorescent and phase-contrast microscopic techniques using affinity-purified antibody to characterized collagens. Type IV mouse tumor procollagen and type V collagen are present within the mesangium and along the endothelial aspect of the glomerular basement membrane, demonstrating antigenic continuity between these two glomerular zones. In contrast, antiserum to type IV collagen from bovine lens capsule reacts with the full thickness of the glomerular basement membrane, as well as in the tubular basement membrane and Bowman's capsule. Types I procollagen and collagen are found within the epithelium; and types III collagen and procollagen are not detected within the glomerulus. The functional role of the multiple distinct "basement membrane" collagens in the glomerular capillary wall is unknown. A complex structure is suggested, especially in the mesangial-subendothelial continuity, and in the differential localization of two forms of type IV collagen within the glomerular basement membrane. [less ▲]Detailed reference viewed: 8 (0 ULg)
Immunolgic response to (pre)neoplastic cervical lesions associated with human papillomavirus
in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2005), 160(5-6), 287-93Detailed reference viewed: 9 (0 ULg)
Immunolocalization of laminin, fibronectin, and collagens I to V in the normal and mastotic breast
; Foidart, Jean-Michel ;
in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1982), 11(5), 549-54
Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the ... [more ▼]
Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the lactiferous ducts. In consists of a study of 35 samples which were taken from 13 women whose ages ranged from 40 to 75 and whose breasts were considered on clinical examination and on radiography as normal or abnormal. We have analyzed the distribution of anti-collagen types I, III, IV and V and anti-fibronectin and anti-laminin antibodies in certain sites in the breast which were chosen because of the diversity of forms they could take from one woman to another. In each case of the distribution of protein markers seemed to be regular without, as can be found in certain cases of scar or tumour formation, a change in the distribution of the matrix proteins. Therefore, as already suggested by certain histologists, benign abnormal breast conditions (fibrosis) should be considered as simple variations from the normal. This conclusion agrees with classical histological findings. [less ▲]Detailed reference viewed: 19 (1 ULg)
An Immunologic investigation of canine eosinophilic bronchopneumopathy.
Clercx, Cécile ; Peeters, Dominique ; et al
in Journal of Veterinary Internal Medicine (2002), 16Detailed reference viewed: 8 (3 ULg)
The immunological 'self' of neuroendocrine protein families
in Journal of Neuroimmunology (1994), 54Detailed reference viewed: 4 (0 ULg)