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See detailIn vitro investigations of smart drug delivery systems based on redox-sensitive cross-linked micelles
Cajot, Sébastien; Schol, D.; Dahnier, F. et al

in Macromolecular Symposia (2013), 13(12), 1661-1670

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly ... [more ▼]

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly(α-azide-ϵ-caprolactone). Stability of these micelles is insured in diluted media by cross-linking their core via the addition of a bifunctional cross-linker, while redox sensitivity is provided to these micelles by inserting a disulfide bridge in the cross-linker. The potential of these responsive micelles to be used as nanocarriers is studied in terms of cytotoxicity and cellular internalization. The release profiles are also investigated by varying the environment reductive strength. [less ▲]

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See detailIn Vitro Kinetics of a Newborn Rat Astroglia-Derived Neuronotoxic Activity
Leprince, Pierre ULg; rigo, Jean-Michel; Lefebvre, Philippe ULg et al

in Neuroscience Letters (1989), 102(2-3), 268-72

A low-molecular weight astrocyte-derived neuronotoxic activity (ANTA) was detected, using a colorimetric bioassay of cell survival, by its effect on cultured granule cells. This neuronotoxic activity was ... [more ▼]

A low-molecular weight astrocyte-derived neuronotoxic activity (ANTA) was detected, using a colorimetric bioassay of cell survival, by its effect on cultured granule cells. This neuronotoxic activity was found to be released rapidly from newborn rat astrocytes in culture upon incubation in 50 mM K+-containing growth medium. The release by astrocytes could be induced repetitively by successive incubations in high-K+ medium alternating with incubations in normal medium. Astrocytes were also found to inactivate rapidly isobutanol-extracted ANTA in normal K+-containing growth medium. Kinetic studies showed that ANTA induces a slow (greater than 12 h) degeneration of cultured granule cells. ANTA is shown here to be an intermediate of normal astrocyte metabolism and to display appropriate kinetic characteristics compatible with its proposed role in inducing part of the delayed neuronal loss that occurs after a brain injury (secondary neuronal death). [less ▲]

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See detailIn vitro maturation treatment affects developmental competence of laparoscopic ovum pickup-derived oocytes in follicle-stimulating hormone-stimulated goats
Locatelli, Y; Poulin, N; Baril, G et al

in Reproduction, Fertility, & Development (2008), 20(1), 182-183

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU ... [more ▼]

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU sessions were performed on 33 adult goats of the Saanen and Alpine breeds. Females were synchronized (Day 0) during the nonbreeding season by inserting vaginal sponges (45 mg of fluorogestone acetate, Intervet, Boxmeer, The Netherlands). At Day 8, an i.m. injection of 50 μg of cloprostenol (Estrumate; Schering-Plough Animal Health, Pointe-Claire, Quebec, Canada) was administered. Porcine FSH (Stimufol, Merial, Brussels, Belgium, 160 mg/goat) was administered in 5 injections at 12-h intervals, starting on Day 8. The LOPU took place under general anesthesia on Day 11, and follicles ≥2 mm were aspirated with an 18-gauge needle connected to a controlled vacuum system. Vaginal sponges were removed at the time of LOPU. Treatments were repeated 2 times in a 2-week interval scheme (2 goats and 1 goat were excluded from the experiment during the second and third LOPU sessions, respectively). Cumulus–oocyte complexes were washed and evaluated for quality (graded from 1 to 3). Oocytes recovered from unstimulated slaughterhouse-derived ovaries served as a control. Cumulus–oocytes complexes from Grades 1 and 2 were submitted to IVM in TCM-199, supplemented with 100 μm of cysteamine and either 10 ng mL–1 of epidermal growth factor (EGF) or 10% follicular fluid and 100 ng mL–1 of ovine FSH (FF-FSH). Matured oocytes were then submitted to IVF and in vitro development as described by Cognié et al. (2004 Reprod. Fertil. Dev. 16, 437–445). Over the 94 LOPU sessions, 20.4 ± 0.9 follicles were aspirated (mean ± SEM), allowing the recovery of 12.3 ± 0.7 COC per goat and per session, of which 80.1% were suitable for IVM (Grades 1 and 2). Results of in vitro production are detailed in the table. The IVM treatment did not significantly affect cleavage or blastocyst development rates in oocytes derived from slaughterhouse ovaries. Cleavage rates were significantly decreased in LOPU-derived oocytes when compared with control oocytes. For LOPU-derived oocytes, cleavage and final blastocyst development rates were increased significantly and kinetics of embryo development were accelerated when FF-FSH was used during IVM as compared with EGF. The IVM with FF-FSH allowed us to produce 4.1 blatocysts per goat per LOPU session. These results demonstrate the interest in LOPU for goat embryo production once appropriate IVM treatment is used. The difference observed between LOPU and slaughterhouse oocytes in terms of response to IVM treatments may be related to FSH stimulation prior to the LOPU session or to postmortem changes in oocyte responsiveness in the slaughterhouse group. [less ▲]

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See detailIn vitro micropropagation of Jatropha curcas L. from bud aggregates
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Druart, Phillipe et al

in Journal of Technology Innovations in Renewable Energy (2013), 2

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 ... [more ▼]

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 mg.l-1 adenine sulfate, 15 mg.l-1 L-arginine, 2.46 µM IBA (indole-3-butyric acid), 30 g.l-1 sucrose and 7 g.l-1 of agar, and enriched with different balances of BA (benzyladenine) and L glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 µM BA and 25 mg.l 1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in a MS medium supplemented with 2.21 µM BA, 5.70 µM IAA (indole-3-acetic acid) and 15 mg.l-1 L arginine. These shoots were isolated and then rooted in MS containing 2.46 µM of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable. [less ▲]

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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2013, December 06)

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers ... [more ▼]

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers like baby bottle, cups, etc. BPA can migrate into food in contact with polycarbonate. There is a worldwide concern about BPA because several studies have shown endocrine disruptor potency of BPA causing possible adverse health effects. In January 2011, the European Commission decided to ban the use of polycarbonate to manufacture baby feeding bottles. In a recent opinion, the Superior Health Council’s issued its concern regarding the currently use of alternatives to polycarbonate in these materials. This work is part of the ALTPOLYCARB project, which aims to study the migration products from non polycarbonate plastic material, and their endocrine disruptor activities. The endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles will be explored using cell based transactivational assays also named “reporter gene assays”. The MCF7 recombinant cells used here (named MCF7-ER cells) are genetically modified cells containing the firefly luciferase gene, as a reporter gene, and a DNA responsive element specific to the human estrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances, which were shown to migrate from plastic baby bottles, in a recent study performed by Simoneau & al, 2012 . Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7-ER cells. After the first screening, some substances clearly show an activity such as BPA, benzophenone, 2-propenoic acid-2-ethylhexyl ester, benzaldehyde-4-methylthio, butylated hydroxytoluene and dodecanoic acid methyl ester, whereas others ask an in-depth analysis to confirm their activity. For active substances only, the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity. [less ▲]

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See detailIn vitro modelisation of prions neuroinvasion mediated by dendritic cells
Dorban, G.; Defaweux, Valérie ULg; Heinen, Ernst ULg et al

Poster (2008, October)

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See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Labasse, A; Zheng, SX et al

in Rheumatology in Europe (1998), 27(S2), 7

Detailed reference viewed: 10 (3 ULg)
See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Reginster, Jean-Yves ULg

in Reginster, Jean-Yves; Pelletier, J-P; Martel-Pelletier, J (Eds.) et al Osteoarthritis: Clinical and experimental aspects (1999)

Detailed reference viewed: 9 (2 ULg)
See detailIn vitro models of non persistent and persistent infection of human and murine neuroblastoma cell lines by the varicella zoster virus
Schlabertz, Tania; Sadzot-Delvaux, Catherine ULg; Piette, Jacques ULg et al

in Archives Internationales de Physiologie et de Biochimie (1997)

Detailed reference viewed: 38 (4 ULg)
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See detailIn vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL.
Van Heusden, Alain ULg; Nusgens, Betty ULg; Goffinet, Gerhard ULg et al

in Journal of Periodontal Research (1998), 33(6), 377-85

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce ... [more ▼]

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium. [less ▲]

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See detailIn vitro modulation of human gingival keratinocyte migration by minocycline-HCl
Van Heusden, Alain ULg; Nusgens, Betty ULg; Lapière, Charles et al

in Journal of Dental Research (1998), 77

Detailed reference viewed: 18 (3 ULg)
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See detailIn vitro modulation of keratinocyte attachment by minocycline H-Cl
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

in Journal of Dental Research (1995), 74

Detailed reference viewed: 17 (3 ULg)
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See detailIn vitro modulation of keratinocyte attachment by minocycline HCl.
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

Poster (1994)

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See detailIn vitro modulation of keratinocyte attachment to dentin by minocycline
Van Heusden, Alain ULg; Rompen, Eric ULg; Lapière, Charles

in Journal of Dental Research (1997), 76

Detailed reference viewed: 16 (3 ULg)
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See detailIn vitro modulation of radiosenitizing effect of FMdC. The importance of simultaneous alteration of the novo and salvage pathways to deoxyribonucleosides.
COUCKE, Philippe ULg; Cottin, E; Ciernick, I-F et al

in International Journal of Radiation, Oncology, Biology, Physics (2000), 46(3),

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See detailIn vitro multiplication of an industrial fiber plant: kenaf (Hibiscus cannabinus L.)
Arbaoui, Sarra; Campanella, Bruno ULg; Paul, Roger ULg et al

Poster (2012, April 23)

Detailed reference viewed: 118 (8 ULg)
See detailIn vitro neuroblastoma cell infection by varicella-zoster virus
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Piette, Jacques ULg et al

Poster (1988)

Detailed reference viewed: 9 (0 ULg)