Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailThe Influence of Phylogenic Origin on the Occurrence of Brachycephalic Airway Obstruction Syndrome in a Large Retrospective Study
Njikam Nsangou, Ibrahim ULg; Huault, Maxime; Pirson, Vincent et al

in International journal of applied research in veterinary medicine (2009), 7(3), 138-143

Strategies to identify genetic polymorphisms responsible for inherited disorders in purebred dog population are often based on experimental studies whereas hospitals case reports provide widely and easily ... [more ▼]

Strategies to identify genetic polymorphisms responsible for inherited disorders in purebred dog population are often based on experimental studies whereas hospitals case reports provide widely and easily affordable information of clinical significance. When this clinical information is enriched with knowledge on the phylogenetic origins, breed histories of the dog and adjusted for known biases, they may lead the way to more in depth genetic research. We illustrated here with a very complex disease, the brachycephalic airway obstruction syndrome (BAOS). No cases were reported in the boxer breed while almost half of the dogs from the other brachycephalic breeds of the same phylogenetic cluster were BAOS, making these breeds good candidates for further genetic studies. Critical issues to improve utility of clinical data for genetic studies are discussed along with methods to handle biases inherent to such type of studies. [less ▲]

Detailed reference viewed: 166 (7 ULg)
Full Text
Peer Reviewed
See detailInfluence of physico-chemical characteristics of limestone fillers on fresch and hardened mortar performances
Michel, Frédéric ULg; Piérard, Julie; Courard, Luc ULg et al

in De Schutter, G.; Boel, V. (Eds.) Self-Compacting Concrete SCC 2007 (2007, September)

In order to meet the specific requirements for fresh Self-Compacting Concrete (SCC), i.e. a high workability together with a good resistance to segregation, the amount of coarse aggregates has to be ... [more ▼]

In order to meet the specific requirements for fresh Self-Compacting Concrete (SCC), i.e. a high workability together with a good resistance to segregation, the amount of coarse aggregates has to be reduced and replaced by fine material. Since cement is quite expensive and can develop a high heat of hydration with possible problems for thermal cracks in massive concrete, mineral fillers are usually used. In Belgium, local available materials are limestone fillers; they are very well-adapted for the optimisation of particle packing and flow behaviour of cementitious paste in SCC mixes. These by-products are issued from different sectors, such as the aggregate and lime production industry (quarrying operations) and the ornamental stones industry (sawing operations). The suitability of these fillers for use in SCC or conventional concrete production was investigated. This paper reports the effect of the nature and the substitution rate of the fillers on the properties of mortars. Some relationship between the physico-chemical properties of the fillers and the properties of mortars were brought forward. [less ▲]

Detailed reference viewed: 111 (9 ULg)
Full Text
Peer Reviewed
See detailInfluence of Plasminogen Activator Inhibitor Type 1 on Choroidal Neovascularization
Lambert, Vincent ULg; Munaut, Carine ULg; Frankenne, F. et al

in FASEB Journal (2001), 15(6), 1021-7

High levels of the plasminogen activators, but also their inhibitor, plasminogen activator inhibitor 1 (PAI-1), have been documented in neovascularization of severe ocular pathologies such as diabetic ... [more ▼]

High levels of the plasminogen activators, but also their inhibitor, plasminogen activator inhibitor 1 (PAI-1), have been documented in neovascularization of severe ocular pathologies such as diabetic retinopathy or age-related macular degeneration (AMD). AMD is the primary cause of irreversible photoreceptors loss, and current therapies are limited. PAI-1 has recently been shown to be essential for tumoral angiogenesis. We report here that deficient PAI-1 expression in mice prevented the development of subretinal choroidal angiogenesis induced by laser photocoagulation. When systemic and local PAI-1 expression was achieved by intravenous injection of a replication-defective adenoviral vector expressing human PAI-1 cDNA, the wild-type pattern of choroidal angiogenesis was restored. These observations demonstrate the proangiogenic activity of PAI-1 not only in tumoral models, but also in choroidal experimental neovascularization sharing similarities with human AMD. They identify therefore PAI-1 as a potential target for therapeutic ocular anti-angiogenic strategies. [less ▲]

Detailed reference viewed: 34 (4 ULg)
Full Text
Peer Reviewed
See detailInfluence of players' level on racket speed and ball accuracy in the tennis serve
Tubez, François ULg; Croisier, Jean-Louis ULg; Cordonnier, Caroline ULg et al

Conference (2014, July 06)

INTRODUCTION Serve in modern tennis game is an important offensive weapon for players (1-2). In kinematic analysis, serve is the most studied stroke of this game. The aim of our study was to compare the ... [more ▼]

INTRODUCTION Serve in modern tennis game is an important offensive weapon for players (1-2). In kinematic analysis, serve is the most studied stroke of this game. The aim of our study was to compare the performance of two specific populations: international players versus national players. In particular, racket speed at impact and accuracy of ball were assessed. METHODS A tennis court was reconstructed in a motion analysis laboratory. The position of the racket was evaluated in 3D at a frequency rate of 200 Hz. Tests were performed on 6 professional players (international level) and 9 non-professional players (national level). Each of them served 25 trials in direction of the “T” area of deuce diagonal. Two squares of 1m² and 2 m² respectively were delimited on the corner of the serve square. The instruction for both groups was to serve in the “T” area with the highest ball speed and minimal ball rotation (flat serve). RESULTS Although the forward speed of the racket at impact was identical between the two groups of players (International 36.35 ± 2.37 m/s and national 36.37 ± 2.90 m/s, p-value 0,991), the accuracy and consistency of serves on the target area is better for international players group (1m² area: International 33% ± 7% and national 14% ± 12%, p-value 0.0053; 2m² area (including 1m² area): International 71% ± 8% and national 54% ± 12%, p-value 0.0096; Out of zone: International 29% ± 8% and national 46% ± 12%, p-value 0.014). DISCUSSION High-velocity ball seems to be a key factor for serve performance (3). It is known that there is a relationship between racket speed and ball velocity (4). Both groups have high racket speed. However, international players serve with better accuracy and consistency than national players. We hypothesize that these differences are due to capacity of international players to adapt to a particular environment. Moreover, international players could give priority to consistency over velocity. We conclude that high-velocity serve is not a sufficient criterion to perform at international level; consistency and accuracy are two important factors to reach this level. [less ▲]

Detailed reference viewed: 82 (25 ULg)
Full Text
Peer Reviewed
See detailInfluence of practice characteristics on injury risk in young athletes
Frisch, Anne ULg; Urhausen, Axel; Seil, Romain et al

in British Journal of Sports Medicine (2011), 45

Detailed reference viewed: 47 (7 ULg)
Full Text
Peer Reviewed
See detailInfluence of processing parameters on physicochemical properties of low-trans and trans-free puff pastry margarines
Lefebure, Emilie ULg; Ronkart, Sébastien; Brostaux, Yves ULg et al

in LWT - Food Science and Technology (2013), 51(1), 225-232

Detailed reference viewed: 38 (21 ULg)
Full Text
Peer Reviewed
See detailInfluence of progesterone concentrations on secretory functions of trophoblast and pituitary during the first trimester of pregnancy in dairy cattle
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Theriogenology (2007), 67(9), 1503-1511

The essential role played by progesterone in the maintenance of pregnancy is unequivocal; however, the effects of progesterone on the secretory patterns of placental and pituitary molecules during the ... [more ▼]

The essential role played by progesterone in the maintenance of pregnancy is unequivocal; however, the effects of progesterone on the secretory patterns of placental and pituitary molecules during the gestation period are not well defined. The objective of this study was to describe pregnancy-associated glycoprotein (PAG) concentrations (measured by RIA-497 and RIA-Pool) in pregnant females with progesterone concentrations lower (low-P4 group, n = 20) or higher (high-P4 group, n = 17) than the mean of 8.74 ng/mL on Day 21 (AI = Day 0). Luteinizing hormone (LH) and prolactin concentrations were also measured in both groups. Throughout the study period, blood samples were collected on Days 0, 21, 45, 60, and 80 from 37 females that were confirmed to be pregnant. PAG concentrations measured by both RIA-497 and RIA-Pool tended to be higher in high-P4 group than in low-P4 group from Day 30 until Day 80. On Day 80, plasma PAG concentrations that were measured using RIA-497 were observed to be higher (P < 0.05) in the high-P4 group than in the low-P4 group (10.2 +/- 8.7 ng/mL versus 6.9 +/- 13.8 ng/mL). Concentrations of LH on Day 60 and prolactin on Day 80 were observed to be significantly lower (P < 0.05) in the high-P4 group. There was a tendency for the concentrations of LH (Days 45 and 80) and prolactin (Days 30, 45, and 60) to be lower in cows in the high-P4 group than in the low-P4 group. Our results suggest the existence of a relationship among the concentration levels of progesterone, PAG, LH, and prolactin during early pregnancy. (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 27 (1 ULg)
Full Text
See detailInfluence of protein context on the propensity of polyglutamine tracts to induce protein aggregation into amyloid fibrils
Huynen, Céline ULg

Poster (2012, April 18)

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of ... [more ▼]

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of the CAG codon in the corresponding genes, and are present in proteins of healthy people. They are however pathogenic when their length, due to mutations, becomes higher than a threshold generally comprised between 35 and 45Q. Such pathogenic tracts trigger the aggregation of the proteins into amyloid-like aggregates that could play an important role in the disease. It is therefore necessary to investigate at a molecular level the aggregation process of polyQ proteins. Since proteins associated with polyQ diseases are generally big and relatively insoluble, they are difficult to produce and manipulate. We have therefore decided to study the aggregating properties of polyQ proteins by designing and characterizing model proteins made of a well-characterized host protein, the β-lactamase BlaP, and polyQ tracts of different lengths (23 to 79Q) inserted at position 197 of BlaP. The aggregating properties of these BlaP chimeras recapitulate those of proteins associated with polyQ diseases. We indeed observed that there is a minimal number of glutamines (threshold) required for chimeras aggregation into amyloid-like fibrils and that the kinetics of aggregation are faster with longer glutamine repeats. Most importantly, the value of the threshold for amyloid-like fibril formation seems to critically depend on the structural integrity of BlaP and thus on the constraints applied to the polyQ tract. In the present work, we investigate more deeply the role of the protein context and the role of oligomers in the process of aggregation of BlaP chimeras. This study is mainly based on the use of two techniques: the quartz crystal microbalance with dissipation (QCM-D) in combination with atomic force microscopy (AFM), and the dynamic light scattering (DLS). Preliminary QCM-D results indicate that in native condition there is a minimal number of glutamines required for the elongation of BlaP197(Gln)79 fibrils by BlaP chimeras. This threshold is different to that observed for the aggregation monitored in solution in absence of seed. Most interestingly it corresponds to the threshold observed in denaturing conditions. These observations suggest that the conformation of BlaP is the limiting step for amyloid fibril formation by interfering with the nucleation step. Preliminary DLS experiments indicate that the oligomers formed by BlaP197(Gln)79 in native condition are on the pathway of amyloid fibril formation and could act as aggregation nuclei. [less ▲]

Detailed reference viewed: 31 (3 ULg)
Full Text
See detailInfluence of protein context on the propensity of polyglutamine tracts to trigger protein aggregation into amyloid fibrils
Huynen, Céline ULg

Poster (2012, September 10)

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of ... [more ▼]

Nine neurodegenerative diseases, referred to as polyglutamine diseases, are associated with nine proteins containing an expanded polyglutamine (polyQ) tract. PolyQ tracts are encoded by a repetition of the CAG codon in the corresponding genes, and are present in proteins of healthy people. They are however pathogenic when their length, due to mutations, becomes higher than a threshold generally comprised between 35 and 45Q. Such pathological tracts trigger the aggregation of the proteins into amyloid-like aggregates that could play an important role in the disease. It is therefore necessary to investigate at a molecular level the aggregation process of polyQ proteins. Since proteins associated with polyQ diseases are generally large and relatively insoluble, they are difficult to produce and handle experimentally. We have therefore decided to study the aggregation properties of polyQ proteins by designing and characterizing model proteins made of a well-characterized host protein, the β-lactamase BlaP, and polyQ tracts of different lengths (23 to 79Q) inserted either at positions 197 or 216 of BlaP. The aggregation behaviour of BlaP chimeras recapitulate those of proteins associated with polyQ diseases. We indeed observed that there is a minimal number of glutamines (i.e. a threshold) required for the chimeras’ aggregation into amyloid-like fibrils and that the kinetics of aggregation are faster with longer glutamine repeats. Most importantly, the value of the threshold for amyloid-like fibril formation seems to critically depend on the structural integrity of BlaP and thus on the constraints applied to the polyQ tract. In the present work, we investigate more deeply the role of the protein context and of oligomers on the aggregation process of BlaP chimeras. This study is mainly based on the use of two techniques: the quartz crystal microbalance with dissipation (QCM-D) in combination with atomic force microscopy (AFM), and the dynamic light scattering (DLS). QCM-D studies indicate that, under native condition, there is a minimal number of glutamines required for the elongation of BlaP197(Gln)79 fibrils by BlaP chimeras containing a polyQ tract in position 197. This threshold is lower than that observed for the aggregation assays monitored in solution in the absence of seed; it corresponds however to the threshold for fibril formation observed under denaturing conditions. These observations suggest that the conformation of BlaP is the limiting step for amyloid fibril formation by interfering with the nucleation step. BlaP chimeras containing 55Q or more in position 216 of BlaP also elongate BlaP197(Gln)79 fibrils. Interestingly this rate of cross-elongation is faster than the elongation rate observed with BlaP197(Gln)55 and BlaP197(Gln)79. This observation demonstrates that the protein environment of the polyQ tract influences its propensity to form amyloid fibrils. Finally, preliminary DLS experiments indicate that oligomers formed by BlaP197(Gln)79 under native condition are on-pathway of amyloid fibril formation and could act as aggregation nuclei. [less ▲]

Detailed reference viewed: 45 (6 ULg)
Full Text
Peer Reviewed
See detailInfluence of purified dietary fibre on bacterial protein synthesis in the large intestine of pigs, as measured by the gas production technique.
Bindelle, Jérôme ULg; Buldgen, André; Michaux, David et al

in Livestock Science (2007), 109

Microbial fermentation of non-digestible carbohydrates in the pig's large intestine induces a shift of N excretion from urea in urine to bacterial protein in faeces. Experiments were carried out to ... [more ▼]

Microbial fermentation of non-digestible carbohydrates in the pig's large intestine induces a shift of N excretion from urea in urine to bacterial protein in faeces. Experiments were carried out to measure the mineral N incorporation by the pig intestinal microflora using 5 purified carbohydrates in a gas-test: starch (S), cellulose (C), inulin (I), pectin (P) and xylan (X). Fermentation kinetics was modelled. N source in the buffer solution was replaced by 15N labelled NH4Cl. The bacterial N fixation was determined at mid-fermentation, measuring 15N incorporation into the solid phase of the buffer. The bacterial N fixation was higher (Pb0.001) with I and S (19.9 and 18.1 mg N/g incubated DM), compared to P, C and X (8.7, 5.9 and 5.5 respectively). Inulin and S were fermented also more rapidly, even if I (0.081 h−1) and C (0.074 h−1) showed lower half time fractional rate of degradation than S (0.153 h−1), P (0.133 h−1) and X (0.104 h−1). The insoluble dietary fibre content of the substrates was negatively correlated to bacterial N fixation (r=−0.957, P=0.011). The high crude protein content of P (32.5 mg g−1DM) might explain the lower impact of this substrate on bacterial N fixation, despite its rapid fermentation. Beside the proportion of insoluble fibre, the N content and the rate of fermentation seem to be the major factors influencing bacterial protein synthesis. Further studies including ingredients with variable content of indigestible protein and mean retention time in the pig's intestines are necessary. [less ▲]

Detailed reference viewed: 42 (12 ULg)
Full Text
Peer Reviewed
See detailInfluence of residual alpha-amylase activity on the pasting properties of extracted wheat starch.
Lenartz, Jonathan; Sinnaeve, Georges; Massaux, Carine et al

Poster (2005, June)

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailInfluence of response factors on determining equilibrium association constants of non-covalent complexes by electrospray ionization mass spectrometry
Gabelica, Valérie ULg; Galic, Nives; Rosu, Frédéric ULg et al

in Journal of Mass Spectrometry [=JMS] (2003), 38(5), 491-501

A method for determining the equilibrium association constant of a complexation reaction A + B <=> AB by electrospray ionization mass spectrometry is described. The method consists in measuring the ... [more ▼]

A method for determining the equilibrium association constant of a complexation reaction A + B <=> AB by electrospray ionization mass spectrometry is described. The method consists in measuring the relative intensities of the peaks corresponding to A and to AB in equimolar A–B solutions at different concentrations C0. The results are fitted by a non-linear least-squares procedure, with the two variable parameters being the equilibrium association constant Ka and a factor R, defined by I(AB)/I.A/ = R× [AB]/[A]. The factor R is the ratio between the response factors of AB and A, and corrects for the relative electrospray responses of the complex and the free substrate A, mass discrimination of instrumental origin and/or moderate in-source dissociation. The method is illustrated with the following two systems: complexes between a double-stranded 12-base pair oligonucleotide and minor groove binders, and cyclodextrin complexeswith a,!-dicarboxylic acids. For the oligonucleotide complexes, it is found that the response of the complex is not dramatically different to the response of the free oligonucleotide duplex, as the double helix conformation is disturbed by the drug only to a minor extent. In the case of cyclodextrin complexes, these complexes were found to have a much higher response than free cyclodextrin. This may be due to the fact that cyclodextrin is neutral in solution, whereas the complex is charged, but it can also stem from the fact that a significant proportion of the complex is in a non-inclusion geometry. The present method requires the exact determination of the concentrations of the reactants and is applicable to 1 : 1 complexes. [less ▲]

Detailed reference viewed: 60 (5 ULg)
Full Text
Peer Reviewed
See detailInfluence of response prepotency strength, general working memory resources, and specific working memory load on the ability to inhibit predominant responses: A comparison of young and elderly participants
Grandjean, Julien ULg; Collette, Fabienne ULg

in Brain & Cognition (2011), 77

One conception of inhibitory functioning suggests that the ability to successfully inhibit a predominant response depends mainly on the strength of that response, the general functioning of working memory ... [more ▼]

One conception of inhibitory functioning suggests that the ability to successfully inhibit a predominant response depends mainly on the strength of that response, the general functioning of working memory processes, and the working memory demand of the task (Roberts, Hager, and Heron, 1994). The proposal that inhibition and functional working memory capacity interact was assessed in the present study using two motor inhibition tasks (Go/No-Go and response incompatibility) in young and older participants. The strength of prepotency was assessed with a short or long training phase for the response to be inhibited. The influence of working memory resources was evaluated by administering the tasks in full versus divided attention conditions. The effect of working memory load was manipulated by increasing the number of target and distracter items in each task. Results showed no effect of prepotency strength, whereas dividing attentional resources and increasing working memory load were associated with greater inhibitory effects in both groups and for both tasks. This deleterious effect was higher for older participants, except in the working memory load condition of the Go/No-Go task. These results suggest an interactive link between working memory and response inhibition by showing that taxing working memory resources increases the difficulty of inhibiting prepotent responses in younger and older subjects. The additional detrimental effect of these factors on healthy elderly subjects was related to their decreased cognitive resources and to their shorter span size. [less ▲]

Detailed reference viewed: 43 (7 ULg)