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See detailIdentification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages.
Godfroid, F.; Taminiau, Bernard ULg; DANESE, Isabelle ULg et al

in Infection and Immunity (1998), 66(11), 5485-93

Brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. The smooth lipopolysaccharide (S-LPS) has been reported to be an important virulence ... [more ▼]

Brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. The smooth lipopolysaccharide (S-LPS) has been reported to be an important virulence factor of these organisms, but the genetic basis of expression of the S-LPS O antigen has not yet been described. Likewise, the role of the O side chain of S-LPS in the survival of Brucella has not been clearly defined. A mini-Tn5 transposon mutant library of Brucella melitensis 16M was screened by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) directed against the O side chain of Brucella. One mutant, designated B3B2, failed to express any O side chain as confirmed by ELISA, Western blot analysis, and colony coloration with crystal violet. Nucleotide sequence analysis demonstrated that the transposon disrupted an open reading frame with significant homology to the putative perosamine synthetase genes of Vibrio cholerae O1 and Escherichia coli O157:H7. The low G+C content of this DNA region suggests that this gene may have originated from a species other than a Brucella sp. The survival of B. melitensis mutant strain B3B2 in the mouse model and in bovine macrophages was examined. The results suggested that S-LPS or, more precisely, its O side chain is essential for survival in mice but not in macrophages. [less ▲]

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See detailIdentification of the post-maximum lines in the spectrum of Nova (RS) Ophiuchi.
Joy, A. H.; Swings, Polydore ULg

in Astrophysical Journal (1945), 102

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS ... [more ▼]

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS) Ophiuchi obtained between September 2 and November 10, 1933, shows many plausible identifications of lines which were impossible earlier. The atoms now identified, in addition to H, are: He I, He II, C III, [N II], N m, [O I], O III, [O III], [Ne III], [Ne IV], Na I, Si I, Si II, Si III, Si IV, [S II], [S III], [A V], [A X], [A XI], [K IV], [K V], [Ca V], [Ca VI], [Ca VII], [Ca XIII], [Sc VII], [V VIII], Fe II, [Fe II], [Fe IV], [Fe V], [Fe VI], [Fe VII], [Fe X], [Fe XI], [Fe XIV], [Ni XII], [Kr III]. Of these, [A XI] λ 6919, [Ca VII] λ 3688 (and possibly [V VIII] λ 3686), [Sc VII] λ 4823, and [Kr III] λ 6827 are identified for the first time. Considerations of the physical conditions in the solar corona as compared with those in the recurrent novae indicate marked differences, the coronal strata not permitting radiation of forbidden lines in the lower stages of ionization such as are found in the novae. [less ▲]

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See detailIdentification of the post-maximun lines in the spectrum of nova (RS) Ophiuchi.
Joy, A.-H.; Swings, Polydore ULg

in Contributions from the Mount Wilson Observatory (1945), 714

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS ... [more ▼]

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS) Ophiuchi obtained between September 2 and November 10, 1933, shows many plausible identifications of lines which were impossible earlier. The atoms now identified, in addition to H, are: He I, He II, C III, [N II], N III, [O I], O III, [O III], [Ne III], [Ne IV], Na I, Si I, Si II, Si III, Si IV, [S II], [S III], [A V], [A X], [A XI], [K IV], [K V], [Ca V], [Ca VI], [Ca VII], [Ca XIII], [Sc VII], [V VIII], Fe II, [Fe II], [Fe IV], [Fe V], [Fe VI], [Fe VII], [Fe X], [Fe XI], [Fe XIV], [Ni XII], [Zr III]. Of these, [A XI] λ 6919, [Ca VII] λ 3688 (and possibly [V VIII] λ 3686), [Sc VII] λ 4823, and [Kr III] λ 6827 are identified for the first time. Considerations of the physical conditions in the solar corona as compared with those in the recurrent novae indicate marked differences, the coronal strata not permitting radiation of forbidden lines in the lower stages of ionization such as are found in the novae. [less ▲]

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See detailIdentification of the signalling pathways required for interleukin-1 beta stimulation of osteoprotegerin synthesis in osteoblastic cells
Lambert, Cecile; Ribbens, Clio ULg; Bours, Vincent ULg et al

in Journal of Bone and Mineral Research (2003, September), 18(Suppl. 2), 142

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See detailIdentification of the Slow-Flow Dynamics of Transonic Aeroelastic Response from Time-Series Data
Lee, Y. S.; Vakakis, Alexander F.; McFarland, D. M. et al

in 45th Technical Meeting of the Society of Engineering Science, Urbana Champaign, 2008 (2008)

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See detailIdentification of Three-Element Windkessel Model: Comparison of Time and Frequency Domain Techniques
Pochet, T.; Gérard, Paul ULg; Marnette, J. M. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100(3, May-Jun), 295-301

The problem of the parameter identification of the three-element windkessel model is studied. Minimization by least-square technique--LSQ--in time domain and frequential techniques--FFT--are compared ... [more ▼]

The problem of the parameter identification of the three-element windkessel model is studied. Minimization by least-square technique--LSQ--in time domain and frequential techniques--FFT--are compared. Continuous pressure and flow curves were recorded in the proximal aorta of an open chest dog. Comparison shows very high correlations between the parameter estimations obtained by LSQ and FFT methods. However, systematic differences appear between the calculated values, but do not seem to endanger physiological interpretation of the results. [less ▲]

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See detailIdentification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa.
de Chial, Magaly; Ghysels, Bart ULg; Beatson, Scott A. et al

in Microbiology (Reading, England) (2003), 149(Pt 4), 821-31

Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent ... [more ▼]

Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I-III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end. [less ▲]

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See detailIdentification of undeclared sources of animal origin in canine dry foods used in dietary elimination trials
Ricci, Rebecca; Granato, A; Vascellari, M et al

in Journal of Animal Physiology & Animal Nutrition (2013), 97

Failure to respond to commercial limited antigen diets can occur in dogs kept on a dietary trial for the diagnosis of adverse food reaction (AFR). The aim of this study was to assess twelve canine dry ... [more ▼]

Failure to respond to commercial limited antigen diets can occur in dogs kept on a dietary trial for the diagnosis of adverse food reaction (AFR). The aim of this study was to assess twelve canine dry limited antigen diets (eleven novel protein diets and one hydrolysed diet) for potential contamination by ingredients of animal origin not mentioned on the label. The validity of the two methods adopted for the detection of such food antigens was also evaluated. Each dietary product was analysed by microscopy analysis using the official method described in Commission Regulation EC 152/2009 with the aim of identifying bone fragments of different zoological classes (mammalian, avian and fish) and by polymerase chain reaction (PCR) for the identification of DNA of animal origin. Discrepancies between the results obtained by PCR and/or microscopy analysis and the ingredients listed on pet food packages were found. Only in two pet foods did the results of both analyses match the ingredients listed on the label. In the remaining ten samples, microscopy detected bone fragments from one or two unpredicted zoological classes, revealing avian fragments in six of ten samples followed by those of fish in five of ten and mammalian fragments in four of ten. In two samples, microscopy analysis identified a contamination that would have otherwise passed unobserved if only PCR had been used. However, PCR confirmed the presence of all the zoological classes detected by microscopy and also identified the DNA of an additional unexpected zoological class in two samples. Dogs might fail to respond to commercial limited antigen diets because such diets are contaminated with potential allergens. Both PCR and microscopy analysis are required to guarantee the absence of undeclared animal sources in pet foods. Before ruling out AFR, a novel protein home-made diet should be considered if the dog is unresponsive to a commercial regimen. [less ▲]

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See detailIdentification of viable myocardium early after acute myocardial infarction using closed-loop arbutamine echocardiography: comparison with positron emission tomography
PIERARD, Luc ULg; MELON, Pierre ULg; LANCELLOTTI, Patrizio ULg et al

in Acta Cardiologica (2001), 56(6), 387-394

Objective -This study sought to evaluate the safety and efficacy of arbutamine echocardiography in identifying contractile reserve and predicting functional improvement early after acute myocardial ... [more ▼]

Objective -This study sought to evaluate the safety and efficacy of arbutamine echocardiography in identifying contractile reserve and predicting functional improvement early after acute myocardial infarction (AMI). Methods and results - Seventeen patients with first AMI underwent arbutamine echocardiography 48 to 96 hours after AMI. Arbutamine was infused by a closed-loop delivery device. The heart rate slope was 4 beats/min and the heart rate target was 20 beats/min above baseline heart rate. A follow-up echocardiogram was obtained one month later. N- 13 ammonia and F- 18 FDG positron emission tomographic (PET) imaging were performed 6 2 days after AMI, before coronary angiography. Mean duration of arbutamine infusion was 6 2 min. There was no complication and there were no major side effects. Myocardial viability was identified with PET in 15 of the 17 patients. Contractile reserve was observed in 10 patients during arbutamine infusion. Functional recovery was identified in 12 patients. Sensitivity, specificity and accuracy of PET and arbutamine echocardiography for predicting functional recovery were 100%, 40%, 76% and 67%, 80%, 84%, respectively. Conclusions - Low-dose arbutamine stress testing is safe early after AMI. Contractile reserve can be rapidly identified by echo cardiography and is specific, but moderately sensitive for predicting reversible dysfunction. [less ▲]

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See detailIdentification of VZV ORF9p potential cellular partners that could be important for the viral egress.
Lebrun, Marielle ULg; riva, laura; Rambout, Xavier ULg et al

Poster (2015, July 26)

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate ... [more ▼]

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate of the viral kinase ORF47p and that its ORF47p-dependent phosphorylation is important for the secondary envelopment process. We also have identified an acidic cluster (AC) within the protein that is important for its correct localization in the infected cells and for the interaction with ORF47p. The recombinant VZV expressing ORF9p-ΔAC presents an accumulation of capsids in the perinuclear space. ORF9p seems then to play an important role in several steps of the egress process. In this context, we sought to identify cellular partners of ORF9p that might be important for these functions. We performed a yeast two hybrid screen against the human ORFeome 5.1. and picked out 44 candidates among which 5 proteins playing roles in membrane organization and targeting. We currently are trying to confirm these interactions in infected cells and to assess the role of these interactions for the viral lytic cycle. [less ▲]

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See detailIdentification of wire rope isolators using the restoring force surface method
Kerschen, Gaëtan ULg; Lenaerts, Vincent; Golinval, Jean-Claude ULg

in International Conference on Structural Systems Identification, Kassel, 2001 (2001)

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by ... [more ▼]

The restoring force surface method offers an efficient and reliable identification of non-linear single-degree-of-freedom systems. The method may be extended to multidegree-of-freedom systems but by loosing the key advantage of the method which lies in the two-dimensional representation for single-degree-of-freedom systems. An experimental application of the restoring force surface method is considered in the present paper. The structure investigated consists of wire rope isolators mounted between a load mass and a base mass. These helical isolators were found to be characterised by a non-linear behaviour. The results obtained are discussed in details and the advantages and drawbacks of the method are underlined. [less ▲]

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See detailIdentification of yield locus parameters of metals using inverse modeling and full field DIC
Lecompte, D.; Cooreman, S.; Sol, H. et al

in Proceedings of the 7th national congress on theoretical and applied mechanics (2006)

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as ... [more ▼]

The basic principle of the inverse modeling procedure as it is used for parameter identification is the generation of a complex and heterogeneous deformation field that contains as much information as possible about the parameters to be identified. One way of obtaining such a non-homogeneous deformation is by making the geometry of the specimen less regular. Another possibility is to make the loading conditions more complex. In this paper both options are actually combined by using the concept of a biaxial tensile test on a perforated cruciform specimen. In the present paper, the work hardening of the material is assumed to be isotropic and it is described by a Swift law. The yield locus is modeled by the anisotropic Hill48 criterion. The optimization technique used is a constrained gradient based Newton-type routine, which means that in every iteration step, a sensitivity calculation has to be performed in order to indicate the direction in which the parameters are to be optimized. The functional to be minimized is a least-squares expression of the discrepancy between the measured and the simulated strain fields at a certain load. The numerical routines as well as the identification results of the different parameters, based on simulated strain fields, are discussed. [less ▲]

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See detailIdentification of ‘Textsorten’ in the Late Egyptian Corpus
Gohy, Stéphanie ULg; Martin Leon, Benjamin ULg

in Winand, Jean; Polis, Stéphane (Eds.) Texts, Languages & Information. Technology in Egyptology. Selected papers from the meeting of the Computer Working Group of the International Association of Egyptologists (Informatique & Égyptologie), Liège, 6-8 July 2010 (2010, July 08)

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See detailIdentification On Commercialized Products Of Aflp Markers Able To Discriminate Slow- From Fast-Growing Chicken Strains
Fumiere, Olivier; Dubois, Marc; Gregoire, Dimitrie et al

in Journal of Agricultural and Food Chemistry (2003), 51(5), 1115-1119

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc ... [more ▼]

The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc. Most of those certified productions have specifications requiring the use of slow-growing chicken strains. The amplified fragment length polymorphism (AFLP) technique has been used to search molecular markers able to discriminate slow-growing chicken strains from fast-growing ones and to authenticate certified products. Two pairs of restriction enzymes (EcoRI/MseI and EcoRI/TaqI) and 121 selective primer combinations were tested on individual DNA samples from chicken products essentially in carcass form that were ascribed as belonging to either slow- or fast-growing strains. Within the resulting fingerprints, two fragments were identified as type−strains specific markers. One primer combination gives a band (333 bp) that is specific for slow-growing chickens, and another primer pair generates a band (372 bp) that was found to be characteristic of fast-growing chickens. The two markers were isolated, cloned, and sequenced. The effectiveness and the specificity of the two interesting determinants were assessed on individuals of two well-known strains (ISA 657 and Cobb 500) and on commercialized products coming from various origins. [less ▲]

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See detail"Identification organisationnelle et changement: les logiques identitaires à l'épreuve du New Public Management"
Rondeaux, Giseline ULg

Doctoral thesis (2012)

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements ... [more ▼]

Depuis près de 30 ans, nombre de pays se sont engagés dans la réforme de leur administration publique, inspirée du New Public Management (NPM), inspirant de multiples travaux et débats sur ses fondements et ses déclinaisons, ses avantages et inconvénients, sa pertinence, ses paradoxes ou encore les difficultés liées à sa mise en oeuvre ou à son évaluation. Construit comme une critique des règles de l’administration bureaucratique, ce modèle managérial constitue une profonde remise en cause de celle-ci en termes d’organisation, de management, de GRH et de valeurs fondamentales. Les réformes inspirées du NPM sont aussi à la base d’une redéfinition de l’identité organisationnelle institutionnalisée de l’administration (Du Gay, 1996) : l’organisation s’interroge sur ce qu’elle est, ce qu’elle devient, ce qu’elle veut être, propulsant l’identité organisationnelle au premier plan des préoccupations. Alors que le processus de modernisation de l’administration vise à redéfinir la réponse à la question « qui sommes-nous en tant qu’organisation ? », de quelle manière les membres de celle-ci en font-ils l’expérience? Au travers de l’analyse de trois cas de réforme d’administrations publiques, notre thèse s’intéresse aux dynamiques d’identification à l’oeuvre en regard de la perception des évolutions du contexte par les individus. Nous mettons en évidence le lien qui existe entre les processus d’identification organisationnelle et deux dimensions spécifiques à chaque contexte en évolution : le caractère plus ou moins radical de changement d’identité organisationnelle institutionnalisée que suppose la réforme mise en œuvre, et le degré de maturité de ce processus de réforme, ou en d’autres termes la mesure dans laquelle la réforme est plus ou moins perceptible dans la transformation du contexte même. Au départ des différents éléments relevés dans chacun de nos terrains, notre thèse illustre concrètement comment la mise en œuvre d’un management polyphonique du processus de changement peut contribuer de manière positive à l’identification organisationnelle des membres de ces organisations en évolution. Cette approche mène à réfléchir autrement à la gestion du changement au travers de la prise en compte de cette diversité de positionnements identitaires. Elle conduit en outre à une réflexion plus large sur la mise en œuvre du NPM et sur l’administration publique de demain. [less ▲]

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See detailIdentification par empreintes génétiques des espèces animales entrant dans une chaîne alimentaire
Haezebroeck, Valérie; Renaville, Robert ULg; Bertozzi, Carlo et al

in Cinquième Carrefour des Productions animales. Quels systèmes de Productions Animales pour le 21ème siècle? (2000, January 26)

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