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See detailInduce triploidy by heat shock in Eurasian perch, Perca fluviatilis
Rougeot, Carole ULg; Minet, Laurent; Prignon, Christian ULg et al

in Aquatic Living Resources (2003), 16(2, MAR-APR), 90-94

In Eurasian perch (Perca fluviatilis), females grow significantly faster than males. Moreover, gonadal development has a significant negative impact on somatic growth and fillet yield. In order to induce ... [more ▼]

In Eurasian perch (Perca fluviatilis), females grow significantly faster than males. Moreover, gonadal development has a significant negative impact on somatic growth and fillet yield. In order to induce sterility, triploidy induction was attempted by subjecting fertilised eggs to heat shocks. Different combinations of temperature (28, 30, 34, 35 and 36 degreesC), duration (2, 5, 10 and 25 min) and time of shock initiation (TI = 3, 5 and 7 min post-fertilisation) were tested. Flow cytometry analysis was used to assess ploidy level of control and heat-shocked larvae. Low intensity (28-30 degreesC) and long duration (10 and 25 min) shocks lead to significantly higher survival (44 +/- 26%) and triploidisation (71 +/- 26%) rates than high intensity (34-36 degreesC) and short duration (2 and 5 min) shocks (17 +/- 19% and 21 +/- 26%, respectively). The most effective conditions for efficient triploidy induction were low intensity shock of 30 degreesC, applied 5 min post-fertilisation for 25 min. This treatment led to the production of all-triploid populations (100%) with up to 43% survival rate. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Ifremer/IRD/Inra/Cemagref. All rights reserved. [less ▲]

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See detailInduced acute erythema and late pigmentation may not be correlated: In regards to Perera et al. (Int J Radiat Oncol Biol Phys 2005;62:1283-1290)
Nemeskéri, C.; Póti, Z.; Mayer, A. et al

in International Journal of Radiation, Oncology, Biology, Physics (2006), 65(1), 309-310

[No abstract available]

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See detailInduced land subsidence near major river mouths; from Quaternary geology to coupled numerical models
Dassargues, Alain ULg; Baeteman, Cécile

in 7th International Association of Engineering Geology Congress (1994)

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See detailInduced Magnetoelectric Response in Pnma Perovskites
Bousquet, Eric ULg; Spaldin, Nicola

in Physical Review Letters (2011), 107(19),

We use symmetry analysis to show that the G-, C-, and A-type antiferromagnetic Pnma perovskites can exhibit magnetoelectric (ME) responses when a ferroelectric instability is induced with epitaxial strain ... [more ▼]

We use symmetry analysis to show that the G-, C-, and A-type antiferromagnetic Pnma perovskites can exhibit magnetoelectric (ME) responses when a ferroelectric instability is induced with epitaxial strain. Using first-principles calculations we compute the values of the allowed ME response in strained CaMnO(3) as a model system. Our results show that large linear and non-linear-ME responses are present and can diverge when close to the ferroelectric phase transition. By decomposing the electronic and ionic contributions, we explore the detailed mechanism of the ME response. [less ▲]

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See detailInduced sputum - Comparison between isotonic and hypertonic saline solution inhalation in patients with asthma
Cataldo, Didier ULg; Foidart, Jean-Michel ULg; Lau, L. et al

in CHEST (2001), 120(6), 1815-1821

Background: Sputum induction by hypertonic saline solution inhalation is widely used to study airways secretions in patients with asthma. However, hypertonic saline solution is a potent indirect ... [more ▼]

Background: Sputum induction by hypertonic saline solution inhalation is widely used to study airways secretions in patients with asthma. However, hypertonic saline solution is a potent indirect bronchoconstrictor. Study objectives: We studied the validity of isotonic saline solution (0.9%) inhalation as a means to induce sputum by comparing it to hypertonic saline solution (4.5%) inhalation. Patients: Sixteen patients with moderate-to-severe asthma reporting a clinical history of mucus hypersecretion. Methods: Subjects underwent sputum induction twice at 1-week intervals. Saline solution (hypertonic or isotonic) was inhaled For three periods of 5 min. The parameters assessed in sputum samples were cell counts, sodium, eosinophil cationic protein (ECP), and albumin concentrations, osmolality, and pro-matrix metalloproteinase (MMP)-9 activity by zymography. Results: The maximal fall in peak expiratory flow during sputum induction was greater after inhalation of hypertonic saline solution than after inhalation of isotonic saline solution (p < 0.01). Each subject produced analyzable sputum on both visits. There were no statistically significant differences in total and differential sputum cell counts, and the reproducibility coefficients were high for eosinophils and neutrophils when comparing the two methods. Likewise, sputum levels of ECP and albumin as well as sputum pro-MMP-9 activity were not different between the two methods, and were highly reproducible as shown by high intraclass coefficients (Ri) of correlation (0.72, 0.74, and 0.77 for ECP, albumin, and pro-MMP-9, respectively). Sputum sodium concentrations and osmolality were higher after inhalation of hypertonic saline solution (p < 0.05). Conclusion: In patients with moderate-to-severe asthma reporting a clinical history of mucus hypersecretion, inducing sputum by isotonic or hypertonic saline solution inhalation leads to comparable results in eosinophil and neutrophil cell counts and fluid phase mediators/proteins. [less ▲]

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See detailInduced sputum - Towards Normal Values
LOUIS, Renaud ULg; GODINAS, Laurent; SCHLEICH, FLorence ULg

in Loukides, Stelios; Kostikas, Konstantinos; Barnes, Peter J (Eds.) Non invasive assessment of airways inflammation in asthma and COPD (2011)

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See detailInduced sputum in asthma: from bench to bedside.
Bakakos, P.; SCHLEICH, FLorence ULg; Alchanatis, M. et al

in Current medicinal chemistry (2011), 18(10), 1415-22

During recent years there has been a growing interest in using non-invasive biomarkers to understand and monitor the airway inflammation in subjects with respiratory tract disorders and mainly asthma and ... [more ▼]

During recent years there has been a growing interest in using non-invasive biomarkers to understand and monitor the airway inflammation in subjects with respiratory tract disorders and mainly asthma and chronic obstructive pulmonary disease (COPD). Sputum induction is generally a well-tolerated and safe procedure and a European Respiratory Society Task Force has published a comprehensive review on sputum methodology. Induced sputum cell count and, to a lesser extent, mediator measurements have been particularly well validated. In asthma, the sputum and the cell culture supernatant can be used for the measurement of a variety of soluble mediators, including eosinophil-derived proteins, nitric oxide (NO) derivatives, cytokines and remodelling-associated proteins. Sputum eosinophilia (> 3%) is a classic feature of asthma although half of the patients seems to be non eosinophilic. Measuring the percentage of sputum eosinophils has proved to be useful in the clinical arena in helping to predict short term response to inhaled corticosteroids (ICS) and tailor the dose of ICS in the severe patients but there is scope for the application of other induced sputum markers potentially useful in clinical practice. The widespread application of induced sputum in asthma across the spectrum of disease severity has given insight into the relationship between airway function and airway inflammation, proposed new disease phenotypes and defined which of these phenotypes respond to current therapy, and perhaps most importantly provided an additional tool to guide the clinical management of asthmatic patients. To date sputum induction is the only non-invasive measure of airway inflammation that has a clearly proven role in asthma management. [less ▲]

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See detailInduced sputum studies in asthma
LOUIS, Renaud ULg; Chanez, P

in Djukanovic, Ratko; Sterk, Peter J. (Eds.) An Atlas of INDUCED SPUTUM : an Aid for Research and Diagnosis. (2003)

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See detailInduced sputum: not only for research but also for better patient management in asthma and COPD.
LOUIS, Renaud ULg

in Monaldi Archives for Chest Disease = Archivio Monaldi per le Malattie del Torace (2012), 77(1), 5-7

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See detailThe inducible 70,000 molecular/weight heat shock protein is expressed in the degenerating dentate hilus and piriform cortex after systemic administration of kainic acid in the rat.
Armstrong, J. N.; Plumier, Jean-Christophe ULg; Robertson, H. A. et al

in Neuroscience (1996), 74(3), 685-93

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic ... [more ▼]

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic administration of kainic acid. In contrast to previous reports, the present study demonstrates that neurons in the regions most susceptible to seizure-induced cell death accumulate both Hsp70 messenger RNA and protein. Neurons in the denate hilus and piriform cortex contained Hsp70 messenger RNA at 6 h and protein at 12 h. These neutrons contained little or no Hsp70 messenger RNA or protein at 24 h when the majority of cells in these area were pyknotic. Injured neurons in areas such as the parietal cortex, which are less susceptible to seizure-induced cell death, expressed and maintained high levels of Hsp70 messenger RNA and protein at 12 and 24 h. This work suggest that Hsp70 messenger RNA and protein are rapidly and transiently expressed in dying neurons, and contradicts the notion that Hsp70 only accumulates in injured neurons that survive. [less ▲]

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See detailInducible class C beta-lactamases produced by psychrophilic bacteria
Pierrard, Annick ULg; Ledent, P.; Docquier, J. D. et al

in FEMS Microbiology Letters (1998), 161

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See detailInduction and Characterization of Mitochondrial DNA Mutants in Chlamydomonas Reinhardtii
Matagne, René-Fernand ULg; Michel-Wolwertz, M. R.; Munaut, Carine ULg et al

in Journal of Cell Biology (1989), 108(4), 1221-6

In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not ... [more ▼]

In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not divide under heterotrophic conditions. Two such obligate photoautotrophic mutants were shown to lack the cyanide-sensitive cytochrome pathway of the respiration and to have a reduced cytochrome c oxidase activity. In crosses to wild type, the mutations are transmitted almost exclusively from the mating type minus parent. A same pattern of inheritance is seen for the mitochondrial DNA in crosses between the two interfertile species C. reinhardtii and Chlamydomonas smithii. Both mutants have a deletion in the region of the mitochondrial DNA containing the apocytochrome b gene and possibly the unidentified URFx gene. [less ▲]

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See detailThe Induction by Testosterone of Aromatase Activity in the Preoptic Area and Activation of Copulatory Behavior
Balthazart, Jacques ULg; Foidart, Agnès ULg; Hendrick, J. C.

in Physiology & Behavior (1990), 47(1), 83-94

A series of 4 experiments was designed to study the relationships between the activity of the aromatase (AA) in the preoptic area (POA) and the activation by testosterone (T) of copulatory behavior in ... [more ▼]

A series of 4 experiments was designed to study the relationships between the activity of the aromatase (AA) in the preoptic area (POA) and the activation by testosterone (T) of copulatory behavior in gonadectomized male and female Japanese quail. The induction of AA by T in the POA is dose- and time-dependent. Levels of AA seen in sexually mature males are restored in castrated birds by a treatment with 20 to 40 mm silastic T capsules which produce physiological levels of steroid in the plasma. The minimal dose of T (10 mm implant) which reliably restores copulatory behavior approximately doubles the AA in the POA. The induction of AA is significantly larger in males than in females. A significant increase in AA is observed within 16 hours after the start of the treatment with T and the induction is maximal after 48 hours. Activation of copulatory behavior follows a similar time course but occurs with a delay of 24-48 hours. These results thus suggest that, in male quail, the activity of the aromatase in the POA is a limiting factor in the activation of copulatory behavior. This idea is confirmed by direct experimentation using an aromatase inhibitor, androstatrienedione (ATD). If T-treated birds receive at the same time silastic implants filled with ATD, the activation of behavior is suppressed for at least one week. This behavioral inhibition is, as expected, accompanied and very probably caused by the inhibition of the aromatase activity in the preoptic area and anterior hypothalamus. No increase of enzyme activity over the level seen in castrates was actually detected during the first 8 days of exposure to T. A moderate increase in AA was seen on day 16 and is probably responsible for the behavioral activation which was observed at the end of the experiment. [less ▲]

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See detailInduction de l'oestrus chez les génisses en anoestrus fonctionnel
Beckers, Jean-François ULg; Wouters-Ballman, Patricia; Ectors, Francis ULg et al

in Annales de Médecine Vétérinaire (1978), 122

Fuctional anoestrus is frequently encountered during winter months and most specially in beef cattle. Ovaries and genital tract display a resting, dormant conditions. Progesterone, 17-beta-oestradiol and ... [more ▼]

Fuctional anoestrus is frequently encountered during winter months and most specially in beef cattle. Ovaries and genital tract display a resting, dormant conditions. Progesterone, 17-beta-oestradiol and LH as measured by radioimmunoassay remain at a very low level. Insertion of an intravaginal device "Abbovestrol Prid" (progesterone 2.3 g 17-beta-oestradiol 10mg) is successful. During the course of the treatment; progesterone reaches normal (corpus luteum induced) levels; 17-beta-oestradiol which is at first very high decreases to a mean value of 4-5 pg/ml; LH remains at a basal level but rises slowly at the end of the treatment. Removal of the insert is generally (16/20) followed by oestrus after c.a. 48 hours. This oestrus is characterized by a normal hormonal profile. However, a PMSG injection when the insert is removed raises chances of favourable result. [less ▲]

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See detailInduction des chaleurs chez le zebu Goudali : observation de l’oestrus et insémination artificielle.
Pitala, W.; Zongo, M.; Boly, H. et al

Scientific conference (2005)

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See detailInduction et synchronisation de l'oestrus par la PGF2alpha
Hanzen, Christian ULg; Boudry, B.; Drion, Pierre ULg

in Point Vétérinaire (2003), 34(236), 22-23

Hormonal management of reproduction uses treatments that regulate both luteal activity and follicular growth to achieve expulsion in the shortest period of an oocyte capable of being fertilised. Therefore ... [more ▼]

Hormonal management of reproduction uses treatments that regulate both luteal activity and follicular growth to achieve expulsion in the shortest period of an oocyte capable of being fertilised. Therefore, PgF2alpha has been the object of numerous trials either as sin e or repeated injections, alone or in association with other hormones such as gonadorelin. Systematic insemination is more difficult after a single injection because return to ea is variable. A single injection is better for the physiology of follicular growth than the corpus luteum. Use of a double injection of PgF2alpha improves the degree of synchronisation but doesn't affect the ovulation rate. [less ▲]

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See detailInduction expérimentale de la pneumonie pasteurellique chez la chèvre : étude des modifications physiopathologiques
Bakima, M.; Kaeckenbeeck, A.; Meniai, K. et al

in Annales de Médecine Vétérinaire (1991), 135

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See detailInduction of a Serotonin-S2 Receptor Blockade During Early or Late Stage of Acute Respiratory Distress Syndrome in Double-Muscled Calves: A Comparative Study
Genicot, Bruno; Mouligneau, Frédéric; Lindsey, J. K. et al

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (1993), 40(4), 241-248

Ninety-four Belgian White and Blue double-muscled calves were involved in this study which aimed to compare the efficacy of a serotonin-S2 receptor blockade at two different stages of an acute respiratory ... [more ▼]

Ninety-four Belgian White and Blue double-muscled calves were involved in this study which aimed to compare the efficacy of a serotonin-S2 receptor blockade at two different stages of an acute respiratory distress syndrome (ARDS), i.e. at the occurrence of first clinical signs or when another anti-inflammatory compound was clinically shown to be ineffective. Metrenperone, a 5-hydroxytryptamine (5-HT2) blocker, was injected (intramuscularly, 5 times, at 12 hourly intervals, dose rate: 0.1 mg/kg) to (1) 58 calves referred to our laboratory after a treatment (group I) which lasted from 3 to 5 days and which did not improve the clinical status of the animals and (2) 36 calves investigated as soon as first clinical signs occurred (group II). Following the severity of the ARDS, the animals from group I needed to be classified into 2 groups: group IA (moderate ARDS - n = 45) and group IB (severe ARDS - n = 13). For animals in group I, the antibacterial compound previously used was maintained and administered during 4 more days whilst the previous anti-inflammatory drug was suppressed and replaced by metrenperone administered as mentioned above. In group II, each animal was treated with ceftiofur sodium as an antibacterial agent. A clinical score and a breathing score were calculated at each step of the investigation period, i.e. before (T0) and 1 hour (T1) after the first treatment, during the interval 12H-48H (T2) which followed this treatment and 168 H (T3) after this treatment. Four clinical parameters were also taken into account separately: rectal and cutaneous temperatures, respiratory and heart rates.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailInduction of a Streptomyces Cacaoi Beta-Lactamase Gene Cloned in S. Lividans
Lenzini, V. M.; Magdalena, J.; Fraipont, Claudine ULg et al

in Molecular & General Genetics [=MGG] (1992), 235(1), 41-8

The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was ... [more ▼]

The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of beta-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C beta-lactamase) genes in several Enterobacteria. [less ▲]

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