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See detailImmunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumor cells.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in European Journal of Cell Biology (1988), 47(2), 346-57

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU ... [more ▼]

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus. [less ▲]

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See detailImmunoendocrinology in Health and Disease
Geenen, Vincent ULg; Chrousos, George

Book published by Marcel Dekker (2004)

L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires ... [more ▼]

L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires, infectieuses, allergiques et néoplasiques, de même que le processus d'immunosénescence. Des experts internationaux de chaque domaine ont rédigé des chapitres à propos de maladies chroniques qui ont un impact majeur tant émotionnel qu'économique sur la société humaine. [less ▲]

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See detailImmunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
Kimata, K.; Foidart, Jean-Michel ULg; Pennypacker, J. P. et al

in Cancer Research (1982), 42(6), 2384-91

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has ... [more ▼]

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation. [less ▲]

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See detailImmunofluorescence study of the antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas.
Burtin, P.; Chavanel, G.; Foidart, Jean-Michel ULg

in Annals of the New York Academy of Sciences (1983), 420

Twenty-three colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane (type IV collagen, laminin, and heparan sulfate-rich proteoglycan) as well ... [more ▼]

Twenty-three colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane (type IV collagen, laminin, and heparan sulfate-rich proteoglycan) as well as antisera against antigens of the connective tissue (type III collagen, fibronectin, and hyaluronectin). Marked alterations of the basement membranes were consistently observed on staining with each one of the first three antisera. In contrast, staining of the normal components of connective tissue was in most cases as intense in tumors as in normal colonic mucosa. Hyaluronectin, a marker of peritumoral stroma, was found to be present in 13 out of 16 tumors studied. In six metastatic lymph nodes, tumor foci were sometimes surrounded by antigens of the basement membrane. But these antigens were never found in the cytoplasm of cancer cells. [less ▲]

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See detailImmunogenetics as a tool to assess Hantavirus risk in wild rodents
Guivier, Emmanuel; Galan, Maxime; Pagès, Marie ULg et al

Conference (2011, July)

Immunogenetics, the analysis of genetic polymorphisms in specific recognition and immune regulation, is at the core of the study of host-parasite coevolution. We present here a synthesis of our recent ... [more ▼]

Immunogenetics, the analysis of genetic polymorphisms in specific recognition and immune regulation, is at the core of the study of host-parasite coevolution. We present here a synthesis of our recent works based on immune gene polymorphism to illustrate the power of this approach in improving our knowledge of rodent / hantavirus interactions. Two candidate genes (encoding Mx2 protein and β3 integrin) were first investigated at the inter-specific level. Sequence and/or expression polymorphism were compared among twenty murid species, some of them being reservoirs and some others being non reservoirs of hantaviruses. Our results showed that SNPs as well as patterns of selection acting on these genes differed between these rodent species and could mediate their susceptibility to hantaviruses. Four candidate genes (Mx2 gene and three genes from the Major Histocompatibility Complex) were next analyzed among populations of bank voles (Myodes glareolus), the reservoir of Puumala virus (PUUV). We revealed the existence of positive associations between Mhc alleles and PUUV infection in one hand and negative relationships between Tnf-α or Mx2 expression levels and PUUV prevalence in the other hand. These results suggested that tolerance to PUUV could have evolved in endemic areas. Such tolerance could be selected in response to the costs of immune responses that are activated against PUUV. We will conclude on the advantages to combine this immune gene candidate approach with more recent analyses based on genome scan, to deepen our investigation of this intra- and inter-specific variability of rodent tolerance / susceptibility to hantaviruses. [less ▲]

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See detailImmunogenicity of infliximab: how to handle the problem?
Baert, Filip; De Vos, Martine; Louis, Edouard ULg et al

in Acta Gastro-Enterologica Belgica (2007), 70(2), 163-70

BACKGROUND: The introduction of infliximab has greatly advanced the therapeutic armamentarium of the inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. Although the benefit/risk ... [more ▼]

BACKGROUND: The introduction of infliximab has greatly advanced the therapeutic armamentarium of the inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. Although the benefit/risk ratio for infliximab is positive, of particular concern has been the problem of immunogenicity ascribed to the chimeric properties of the drug. Antibody formation is associated with allergic reactions and loss of response. AIMS AND METHODS: A literature search was undertaken on the magnitude of the problem of immunogenicity and on the clinical consequences. A survey was conducted about the clinical practice and management of acute and delayed allergic reactions to infliximab in different centres in Belgium. For this, a questionnaire was sent to all members of the Belgian IBD research group (n = 38 belonging to 29 centers). RESULTS AND CONCLUSION: Infusion reactions are important immunologic events induced by the presence of a substantial concentration of antibodies against infliximab (ATI) in the serum. Concomitant immunosuppressive treatment may optimize response to infliximab by preventing the formation of antibodies. Steroid administration prior to an infliximab infusion can further reduce the immunogenicity. Probably the most effective strategy to optimize treatment and avoid immunogenicity is maintenance therapy. If infliximab therapy can be discontinued is yet unclear but when treatment goals have been reached, we feel this should be attempted. In the case of relapse, infliximab should be restarted as maintenance long term. Practical guidelines on how to handle the problem of immunogenicity to infliximab are important for clinicians treating patients with IBD. [less ▲]

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See detailImmunogenicity of semisynthetic human insulin in man. Long-term comparison with porcine monocomponent insulin.
Luyckx, A. S.; Daubresse, J. C.; Jaminet, C. et al

in Acta Diabetologica Latina (1986), 23(2), 101-6

The levels of circulating IgG-insulin antibodies were determined in two groups of diabetic patients before and at 3-month intervals after starting insulin treatment either with monocomponent porcine ... [more ▼]

The levels of circulating IgG-insulin antibodies were determined in two groups of diabetic patients before and at 3-month intervals after starting insulin treatment either with monocomponent porcine insulin (n = 17) or with human semisynthetic insulin (SH) (n = 16). Patients were followed during 15.1 +/- 1.0 and 19.9 +/- 1.1 months, respectively (m +/- SEM). In addition, the quality of metabolic control and residual B-cell function were evaluated in the group under treatment with SHI. The percentage of patients who remained antibody-free after 12-21 months of treatment was 67.75% in the human insulin-treated group and only 25-43% in the one receiving porcine insulin (p less than 0.01). Moreover, insulin antibody titers, when present, were usually lower in subjects treated with human insulin. In SHI-treated patients: metabolic control was excellent during the first months of treatment as evidenced by values of mean daily blood glucose (7.3 +/- 0.6 mmol/l), M-index according to Schlichtkrull (7.4 +/- 2.4) and Hb1c (6.8 +/- 0.6%); residual B-cell function, evaluated at 3-month intervals by a circadian profile of plasma C-peptide did not decrease throughout the study; and a significant deterioration of blood glucose control occurred after 18 months of treatment, which might have been due to a less intensive supervision of the patients by the physicians and/or less careful attention by the patients themselves. This observation confirms the need for a continuous education of the patients regardless of the type of insulin used. [less ▲]

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See detailImmunogenicity profile of a 3.75-Ug hemagglutinin pandemic rH5N1 split virion As03A-adjuvanted vaccine in elderly persons : a randomized trial
Heijmans, S.; De Meulemeester, M.; Reynders, P. et al

in Journal of infection diseases (2011), 203(8), 1054-1062

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See detailImmunoglobulines intraveineuses et maladie hémolytique allo-immune du nouveau-né.
Senterre, Thibault ULg; Viellevoye, Renaud ULg; Rigo, Jacques ULg

in Archives de Pédiatrie (2010), 17(3), 299-300

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See detailImmunogold Labelling of Fatty Acyl Chains
Compère, Philippe ULg; Maneta-Peyret, Lilly; Goffinet, Gerhard ULg et al

in Journal of Immunological Methods (1995), 181(2), 201-9

For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either ... [more ▼]

For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide. [less ▲]

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See detailImmunogold Silver Staining Associated With Epi-Fluorescence For Cucumber Mosaic Virus Localisation On Semi-Thin Sections Of Banana Tissues
Helliot, B.; Panis, B.; Busogoro, Jp. et al

in European Journal of Histochemistry (2007), 51(2),

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See detailImmunogold staining of pituitary hormones in human adenoma biopsies
courtoy, r; Beckers, Albert ULg; Stevenaert, Achille ULg et al

in Biology of the Cell (1985)

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See detailImmunogold staining of pituitary hormones in human adenoma biopsies.
Beckers, Albert ULg; Courtoy, R.; Reznik, Michel ULg et al

in Biology of the Cell (1985)

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See detailImmunohistochemical aid at risk stratification of melanocytic neoplasms.
Quatresooz, Pascale ULg; Arrese Estrada, Jorge ULg; Pierard, Claudine ULg et al

in International Journal of Oncology (2004), 24(1), 211-6

There is increasing awareness of the population about the risk of cutaneous melanoma. In recent years, some improvement was gained in the early recognition of clinical warning signs using dermoscopy. As a ... [more ▼]

There is increasing awareness of the population about the risk of cutaneous melanoma. In recent years, some improvement was gained in the early recognition of clinical warning signs using dermoscopy. As a result, the number of puzzling cases exhibiting limited classical clues for malignancy are submitted to the dermatopathologist. Thus, the risk of microscopic uncertainty or misdiagnosis may be increasing. The aim of the study was to assess retrospectively the contribution of immunohistochemistry in establishing the histological diagnosis of 520 melanocytic neoplasms. According to the disease evolution and the histological presentation, 6 profiles of phenotypical expressions were distinguished. [less ▲]

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See detailImmunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: a new quantitative method.
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Wyns, Christine et al

in Fertility and Sterility (1994), 62(4), 751-9

Objective: To evaluate estrogen receptors (ER) and progesterone receptors (PR) content in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: A recently advanced stereographic computer ... [more ▼]

Objective: To evaluate estrogen receptors (ER) and progesterone receptors (PR) content in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: A recently advanced stereographic computer technology was applied for the investigation of steroid receptors. SETTING: University hospital department of gynecology. PATIENTS: Biopsies of endometrium and typical peritoneal endometriotic lesions were taken from 19 infertile patients with laparoscopically proved endometriosis. Endometrial biopsies were also taken from 15 patients without endometriosis. All of them were untreated. RESULTS: In normal endometrium, the highest concentrations of ER and PR occurred in the epithelial and stromal cells during the late proliferative phase of the menstrual cycle. Estrogen receptor and PR content declined throughout the secretory phase. Progesterone receptor content was found not to be significantly decreased in the stroma during the early secretory phase and quite high in the late secretory phase. In peritoneal endometriotic lesions, the highest concentrations of ER and PR were found during the late proliferative phase. When compared with normal endometrium, a lower ER content ans a similar PR content were observed, and the cyclic changes in peritoneal endometriosis lesions were also similar. CONCLUSION: A new computerized technology for the evaluation of ER and PR in eutopic and ectopic endometrium. Although the ER content was found to be lower in endometriotic tissue when compared with endometrium, the cyclic pattern was similar in both eutopic and ectopic endometrium. Progesterone receptor content was similar in both tissues, except during the late secretory phase in ectopic glandular epithelium in which a high persistent PR content was observed. [less ▲]

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See detailImmunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Donnez, Jacques

in Fertility and Sterility (1997), 68(5), 912-919

Objective: To assess the proliferative activity of eutopic and ectopic endometrium throughout the menstrual cycle and its correlation to steroid receptor content. Design: The immunohistochemical use of Ki ... [more ▼]

Objective: To assess the proliferative activity of eutopic and ectopic endometrium throughout the menstrual cycle and its correlation to steroid receptor content. Design: The immunohistochemical use of Ki 67 was applied to investigate the proliferation index. A recently advanced stereographic computer technology was used to investigate steroid receptors. Setting: University hospital department of gynecology. Patient(s): Biopsies of eutopic endometrium, black and red peritoneal endometriotic lesions, and ovarian endometriomas were taken from infertile patients and classified according to the phase of the cycle. Result(s): In normal endometrium, the glandular proliferation index was highest during the proliferative phase and was statistically significantly reduced during the secretory phase. No proliferative activity was observed in the late secretory phase. No statistically significant differences were found between ectopic endometrium and eutopic endometrium except during the late secretory phase, when proliferative activity was still present in endometriotic tissue. The stromal proliferation index was similar in red lesions, ovarian endometriomas, and eutopic endometrium during the secretory phase. In normal endometrium, the highest concentrations of estrogen receptors (ERs) and P receptors (PRs) occurred in the epithelial and stromal cells during the late proliferative phase of the menstrual cycle. Estrogen receptor and PR content declined throughout the secretory phase. In ectopic endometrium, PR persisted in the glandular epithelium during the late secretory phase. Estrogen receptors persisted in the glandular epithelium and stroma of red peritoneal lesions and ovarian endometriomas during the late secretory phase. Conclusion(s): The high proliferative activity and the persistence of ERs and PRs in the stroma of red lesions and ovarian endometriomas emphasize the primordial role of the stroma in the development of endometriosis and suggest different mechanisms of proliferation control from those observed in eutopic endometrium. [less ▲]

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See detailIMMUNOHISTOCHEMICAL ANALYSIS OF TRANSFORMING GROWTH FACTOR BETA 1 AND ITS SIGNALLING PATHWAYS IN CANINE IDIOPATHIC PULMONARY FIBROSIS
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailImmunohistochemical Aspects of the Fibrogenic Pathway in Nephrogenic Systemic Fibrosis.
Quatresooz, Pascale ULg; Paquet, Philippe ULg; Hermanns-Lê, Trinh ULg et al

in Applied Immunohistochemistry & Molecular Morphology (2010)

Nephrogenic systemic fibrosis (NSF) is a rare gadolinium-dependent disorder of the skin and viscera. The aim of this study was to revisit some immunopathologic clues of NSF, including the characterization ... [more ▼]

Nephrogenic systemic fibrosis (NSF) is a rare gadolinium-dependent disorder of the skin and viscera. The aim of this study was to revisit some immunopathologic clues of NSF, including the characterization of glycosaminoglycans, cell tensegrity, and cell proliferation in the dermis. Immunohistochemistry was done using antibodies directed to vimentin, CD34, Factor XIIIa, calprotectin, alpha-smooth muscle actin, Ulex europaeus agglutinin-1 (UEA-1), and MIB1/Ki67 and to glycosaminoglycans, including CD44 var3, versican, and perlecan. The vimentin+ cell density was markedly increased. The vast majority of them corresponded to CD34+ or Factor XIIIa+ dermal dendrocytes (DD) showing distinct cell tensegrity. CD34+DD were slender, elongated, and usually scattered in the dermis but focally clustered in nodular collections. By contrast, Factor XIIIa+ was plump with squat dendrites showing no evidence for being under mechanical stress. Cells in the vicinity of the microvasculature were rounded and exhibited calprotectin immunoreactivity typical for monocyte/macrophages. The microvasculature highlighted by UEA-1 and alpha-smooth muscle actin looked unremarkable. The cell proliferation highlighted by the MIB/Ki67 immunoreactivity was unusually high (>20%) in the interstitial stromal cells. Stromal cells enriched in versican were plump, abundant, and seemed interconnected each other by a dense network of dendrites. By contrast, the immunolabeling for perlecan and CD44 var 3 was unremarkable. In conclusion, the cell population involved in NSF seemed phenotypically heterogeneous, and its growth fraction was clearly boosted in the skin. The intracellular load in versican was prominent. The aspect of cell tensegrity did not suggest the influence of mechanical stress putting stromal cells under tension in the dermis. [less ▲]

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