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See detailIdentification du profil du consommateur par datamining
Schyns, Michael ULg

Scientific conference (2011, April)

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See detailIdentification du Silurien par Acritarches et Chitinozoaires dans le Boulonnais (Nord de la France): implications géodynamiques et paléogéographiques
Degardin, J. M.; Mansy, J. L.; Le Hérissé, A. et al

Conference (2001)

Detailed reference viewed: 6 (0 ULg)
See detailIdentification du site de fixation d'un facteur de transcription contribuant à la surexpression du gène c-erbB2 dans les cellules tumorales BT-474.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Hodzic, Didier et al

Conference (1996, February 14)

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See detailIdentification et caractérisation de bactériocines produites par deux bactéries lactiques (Enterococcus faecium CWBI-B1430 et Enterococcus mundtii CWBI-B1431) isolées à partir de fromages frais artisanaux péruviens
Aguilar Galvez, Ana Consuelo ULg

Doctoral thesis (2011)

A screening of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity against Listeria monocytogenes was performed. The screening was conducted in 27 traditional artisanal-produced cheeses ... [more ▼]

A screening of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity against Listeria monocytogenes was performed. The screening was conducted in 27 traditional artisanal-produced cheeses obtained from different Peruvian regions. Two thousand two hundred and seventy seven strains (2277) were isolated, of which 20 exhibited bacteriocin-like inhibitory activity, two strains stand out for having higher activity against listeria, as well as stability at pH value of 2-11 and a thermal stability. The two strains were identified using 16 S ribosomal DNA method, and were registered in the GenBank database of NCBI (National Center for Biotechnology Information). These were Enterococcus faecium CWBI-B1430 (accession number EF591817) and Enterococcus mundtii CWBI-B1431 (accession number EF591816). Two of the major factors concerned in a potential health risk associated with the genus Enterococcus were evaluated, and it was demonstrated that they were sensitive to vancomycin and showed no hemolytic activity. Genetic studies showed the presence of four putative bacteriocin genes in both strains, these were enterocin A, enterocin B, enterocin P, and mundticin KS. The reverse transcription-PCR analysis was realized, the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in CWBI-B1430 was showed, while bacteriocin genes enterocin P and mundticin KS was demonstrated in CWBI-B1431. These results are the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii. Also, the expression was studied at different temperatures, unlike the enterocin P, where only a positive signal was obtained at 12 and 37°C. All other enterocin showed positive signal at 12, 30, 37 and 42°C. The production of bacteriocins on a small scale, in both strains was studied. It was investigate the influence of initial pH (4.5, 5.0, 5.5, 6.0, 6.5 and 7.0), temperature (25, 30 and 37°C) and oxygen level (anaerobic, microaerobic and aerobic). The conditions for maximizing the production of bacteriocins are : initial pH of 7.0, at 37 °C, and microaerobic conditions for CWBI-B1430 strain; initial pH of 6.5, at 37 °C, and an aerobic condition for CWBI-B1431. The influence of the source of carbon and nitrogen, as well as their concentration in the bacteriocin production, was studied. The study of the production of short-chain fatty acids by these strains of multiproducing bacteriocins, showed the propionic acid as the most important. The purification study confirmed that the two bacteria in question are multi-producing of bacteriocins, as shown by the obtained chromatograms, showing different molecular weights, different charge and hydrophobicity level. The results of in situ application of the two selected strains or their supernatant does not show the same efficiency as those obtained in vitro. Factors such as temperature and food composition may be the origin of these results. Therefore, we propose the use of both strains in the food industry, for being multi-producing bacteriocins, have broad spectrum of inhibition and because the substances that produce have functional properties that are useful in the food industry. [less ▲]

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See detailIdentification et caractérisation des sous-unités ELP5 et ELP6 du complexe Elongator humain
Gillard, Magali ULg

Doctoral thesis (2013)

Familial Dysautonomia is a neurodegenerative disorder that affects the autonomic and sensory nervous systems. This disease results from loss-of-function mutations of the ELP1-encoding gene. ELP1 is ... [more ▼]

Familial Dysautonomia is a neurodegenerative disorder that affects the autonomic and sensory nervous systems. This disease results from loss-of-function mutations of the ELP1-encoding gene. ELP1 is required for the integrity of the so-called Elongator complex, which is composed of two sub-proteins complexes, namely the core-Elongator (ELP1 to ELP3) and HAP (ELP4 to ELP6). While ELP3 is acting as an acetyltransferase that target multiple substrates such as nuclear histones and cytoplasmic α-tubulin, the ELP4-6 has been recently described as an ATPase. This complex has been initially identified as a component of a hyperphosphorylated RNA polymerase II holoenzyme. As a result, Elongator is involved in transcriptional elongation. Since, other roles have been assigned. Elongator is indeed also required for some tRNA modifications in the cytoplasm and consequently controls translation fidelity. Those molecular functions underlie the capacity of Elongator to regulate cell division, DNA-damage response and cell motility. It is likely that a better understanding of the molecular functions of Elongator will clarify its role in these cellular processes and probably highlight new features. The identity of human ELP1 through ELP4 has been reported but human ELP5 and ELP6 have remained uncharacterized. We therefore initiated a study dedicated to the identification and characterization of both proteins. A biochemical purification of ELP4 was conducted to isolate all associated proteins. Such experimental approach led to the identification of DERP6 and C3ORF75 as human ELP5 and ELP6 subunits of the Elongator complex, respectively. We further investigated the cellular functions of both subunits by combining biochemical analysis and cellular assays. Our results show that DERP6/Elp5 is required for the integrity and the fucntion of Elongator and directly connects ELP3 to ELP4. Our laboratory has previously demonstrated the key role of Elongator in cell migration. As cell motility is required for cell invasion and metastasis, we therefore investigated to which extent Elongator is involved in those processes. The migration and tumorigenicity of melanoma-derived cells are significantly decreased upon Elongator invalidation through Elp1 or Elp3 depletion. Strikingly, DERP6/Elp5 and C3ORF75/Elp6-depleted melanoma cells have similar defects, further supporting the idea that DERP6/Elp5 and C3ORF75/Elp6 are essential for Elongator function. Together, our data identify DERP6/ELP5 and C3ORF75/ELP6 as key players for migration, invasion and tumorigenicity of melanoma cells, as integral subunits of Elongator. [less ▲]

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See detailIdentification et caractérisation des unités géomorphologiques de la plaine alluviale du bassin du Kou de Kokoroué à Bama/Badara (Burkina Faso)
Héma, Belo; Sauret, Elie ULg

Report (2010)

La plaine alluviale du bassin du Kou dans la région des Hauts Bassins au Burkina Faso, se déploie principalement le long de la rivière Kou. Cette étude de caractérisation géomorphologique a permis de ... [more ▼]

La plaine alluviale du bassin du Kou dans la région des Hauts Bassins au Burkina Faso, se déploie principalement le long de la rivière Kou. Cette étude de caractérisation géomorphologique a permis de cartographier l'extension latérale des différentes unités de la plaine alluviale et de caractériser la nature des dépôts. [less ▲]

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See detailIdentification et caractérisation partielle d'un gène de Fasciola hepatica d'intérêt diagnostique
Bossaert, K.; Martin-Alonzo, J. M.; Ruiz de Eguino, A. D. et al

in Annales de Médecine Vétérinaire (2000), 144

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See detailIdentification et clonage des antigènes de diagnostic de la brucellose: perspectives d'application
Limet, J. N.; VO, T. K. O.; Saegerman, Claude ULg et al

in Prevention of brucellosis in the mediterranean countries. Proceedings of the international seminar (1992)

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See detailIdentification et distribution des virus du Lagenaria siceraria (Molina) Standl et Citrullus sp. en Côte d'Ivoire
Agneroh, Thérèse Atcham; Kouadio, Kouakou Théodore ULg; Soro, Koloholotcholofolo et al

in Journal of Animal and Plant Sciences (2012), 13(2), 1578-1770

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See detailIdentification et fréquences des bactéries impliquées dans des processus pathologiques chez les mammifères marins
Boseret, Géraldine ULg

Master of advanced studies dissertation (2003)

Cette étude rassemble les résultats obtenus lors d'analyses bactériologiques effectuées sur les mammifères marins échoués au large des côtes belge et du nord de la France, ainsi que sur des organes de ... [more ▼]

Cette étude rassemble les résultats obtenus lors d'analyses bactériologiques effectuées sur les mammifères marins échoués au large des côtes belge et du nord de la France, ainsi que sur des organes de phoques de la mer Caspienne. Escherichia coli bêta-hémolytique est la bactérie isolée le plus fréquemment des phoques examinés. Chez les cétacés, Aeromonas sp. est prédominant sur les autres genres bactériens identifiés, et est le second genre le plus souvent isolé des pinnipèdes. Le diagnostic bactériologique le plus couramment formulé est celui de septicémie ; le poumon est l'organe le plus souvent infecté. Cette étude a pour objet d'approfondir les données existantes en bactériologie des mammifères marins et de donner de nouveaux outils dans la prévention et le traitement des infections bactériennes de ces animaux, en mettant en évidence l'émergence possible de nouveaux agents pathogènes. [less ▲]

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See detailIdentification et modification de comportements enthousiastes d’enseignants en éducation physique
Cloes, Marc ULg

in Dissertation Abstract International (1988), 49(4), 528-

From the analysis of 879 critical incidents gathered with secondary level students and 300 with secondary level physical education teachers, 19 categories of teacher behavior representing the construct of ... [more ▼]

From the analysis of 879 critical incidents gathered with secondary level students and 300 with secondary level physical education teachers, 19 categories of teacher behavior representing the construct of teacher enthusiasm and 10 categories of non-enthusiastic behaviors were developed. Many similarities have been observed between the categories identified in this study and categories provided by the North-American literature. ... [less ▲]

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See detailIdentification et modification de comportements enthousiastes d’enseignants en éducation physique
Cloes, Marc ULg

Doctoral thesis (1987)

This study is divided in two parts. In the first one, the goal was to identify the behavioural descripteurs of the physical education teacher. Data were collected through the critical incidents technique ... [more ▼]

This study is divided in two parts. In the first one, the goal was to identify the behavioural descripteurs of the physical education teacher. Data were collected through the critical incidents technique in teachers and students. They were used to develop and validate an observational system of PE teacher enthusiam. In the second part, three pre-service PE teachers were involved in an experience aiming to modify their enthusiastic behaviours. The experimental design corresponded to the multiple baseline model accross behaviours. [less ▲]

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See detailIdentification et rôle des médiateurs sonores dans les phases de colonisation et d’installation de poissons dans un environnement corallien
Berten, Laetitia ULg

Doctoral thesis (2014)

La majorité des poissons de récifs coralliens présentent un cycle de vie complexe, caractérisé par une phase larvaire pélagique dispersive en milieu océanique, suivie d’une phase benthique au sein du ... [more ▼]

La majorité des poissons de récifs coralliens présentent un cycle de vie complexe, caractérisé par une phase larvaire pélagique dispersive en milieu océanique, suivie d’une phase benthique au sein du lagon ou sur la pente externe du récif. Ce travail porte sur l'influence du stimulus sonore lors de la colonisation et de l'installation, au sein du récif, des larves de poissons. De récentes études ont montré un effet attracteur du son de récifs sur les larves de poissons et de crustacés. Mais on ignore encore la distance à laquelle les larves sont capables de repérer le son du récif, ni si elles sont attirées par une signature spectrale complexe ou plus simplement par la présence d'un "bruit". Enfin, on ignore si les différents habitats d’un récif peuvent présenter des signatures acoustiques propres, ni si les sons des habitats peuvent guider les larves lors de leur installation au sein du récif. L'étude de mesures de pression sonore le long de trois transects de 2 km effectuées sur la côte nord de l'île de Moorea (Polynésie Française) met en évidence une diminution régulière de l'intensité sonore jusqu'à une distance à la crête de 750-1000 m. Au-delà de cette distance, et jusqu'à 2 km, une augmentation graduelle de la pression sonore est observée. Dès lors, une larve située à 750-1000 m du récif se retrouve confrontée à deux gradients de pression, l'un dirigés vers l'océan et l'autre vers le récif, et devra faire appel à d'autres stimuli pour s'orienter vers ce dernier. Au sein du récif, les sons provenant de 5 habitats de Moorea (crête interne, récif barrière, récif frangeant, chenal et mangrove) ont été étudiés. Les signatures sonores de chaque habitat (hormis crête interne et récif barrière) diffèrent des autres sur une certaine gamme de fréquences, et ces différences sont conservées avec la distance (plusieurs km). De plus, l'étude de l'influence des sons d'habitats et d'un son artificiel sur le comportement des larves met en évidence une modification du comportement chez 16 des 20 espèces testées. Les différentes réponses illustrent deux stratégies d'installation sur base du son: soit une sélection directe de l'habitat, soit un évitement de certains habitats, et donc une sélection "par défaut" des habitats restants. Pourtant, les niveaux de pression sonore des habitats du lagon sont sous les seuils de capacités sonores des juvéniles d'Abudefduf vaigiensis, Acanthurus triostegus, Chaetodon citrinellus, Rhinecanthus aculeatus et Myripristis kuntee. Ce paradoxe nous pousse à remettre en question l'utilisation de l'outil "ABR" (Auditory Brainstem Response) dans les études de perception et d'influence des sons sur le comportement des poissons. [less ▲]

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See detailIdentification et tautologie: L'identité chez Husserl et Wittgenstein
Seron, Denis ULg

in Revue Philosophique de Louvain (2003), 101(4), 593-609

A question common to the sixth Logical Research of Husserl and to Wittgenstein's Tractatus is the question of the status of mathematical equations, and more broadly of judgements of identity. It is that ... [more ▼]

A question common to the sixth Logical Research of Husserl and to Wittgenstein's Tractatus is the question of the status of mathematical equations, and more broadly of judgements of identity. It is that of whether the mathematician expresses propositions, which as such bear a character of possible truth, or, on the contrary, simple rules of substitution useful for calculations. As formulated by Frege, this question can be summarized as follows: does mathematical knowledge exist? In reply to this question Husserl's position may be interpreted as being midway between Kant and Wittgenstein. [less ▲]

Detailed reference viewed: 64 (3 ULg)