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See detailIntracellular damages induced by singlet oxygen are signals for HIV-1 reactivation
Piette, Jacques ULg; Legrand-Poels, Sylvie ULg

in Journal of Free Radicals in Biology & Medicine (1993)

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See detailIntracellular distribution of the ORF4 gene product of varicella-zoster virus is influenced by the IE62 protein
Defechereux, Patricia; Debrus, Serge; Baudoux, Laurence et al

in Journal of General Virology (1996), 77(Part 7), 1505-1513

Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for genes of heterologous viruses, The major regulatory immediate ... [more ▼]

Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for genes of heterologous viruses, The major regulatory immediate-early protein of VZV (IE62) is a transactivator of VZV gene expression, In transfection assays, IE4 has been shown to enhance activation induced by IE62, To investigate the functional interactions underlying this observation, indirect immunofluorescence studies were undertaken to determine whether IE62 could influence IE4 intracellular localization in transfected cells, In single transfections, IE4 was predominantly found in cytoplasm, In cotransfection with IE62, the IE4 localization pattern was altered, with nuclear staining predominating over cytoplasmic staining, This effect was specific to the IE62 protein since the gene products of ORF63 and ORF61, which are also regulatory proteins, did not influence IE4 distribution, The use of IE62 mutants indicated that IE62 influence is independent of its transactivation function and that the integrity of regions 3 and 4 is required, IE62 remained nuclear whether IE4 was present or not, These observations underline differences in the regulation of gene expression between VZV proteins and their herpes simplex virus type 1 homologues, In infected cells, IE4 was only sometimes found to colocalize with IE62 in nuclei, This observation suggests that when all VZV proteins are present, complex interactions probably occur which could diminish the influence of IE62. [less ▲]

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See detailIntracellular free iron content of rat liver tissue after cold ischemia
Pincemail, Joël ULg; Sergent, O.; Detry, Olivier ULg et al

in Transplantation Proceedings (2002), 34(3), 759-761

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See detailIntracellular pH-dependent efflux of the fluorescent probe pyranine in the yeast Yarrowia lipolytica
Aguedo, Mario ULg; Waché, Y.; Belin, J.-M.

in FEMS Microbiology Letters (2001), 200(2), 185-189

8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pHi) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH ... [more ▼]

8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pHi) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH-independent wavelength of 415 nm. A simplified correction of the fluorescent signals was applied, enabling to show for this species a good near-neutral pHi maintenance capacity in a pH 3.9 medium. Octanoic acid, which is known to have toxic effects on yeast, decreased the pHi and increased the 260-nm-absorbing compounds leakage. However, this acid inhibited the fluorescent probe efflux linearly with its concentration suggesting a pHi-dependent efflux of pyranine from cells. © 2001 Federation of European Microbiological Societies. [less ▲]

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See detailThe intracellular tyrosine residues of the ATP-gated P2X(1) ion channel are essential for its function.
Oury, Cécile ULg; Toth-Zsamboki, Emese; Watanabe, Hiroyuki et al

in FEBS Letters (2002)

In this study, we mutated the four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in ... [more ▼]

In this study, we mutated the four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in transfected human embryonic kidney (HEK 293) cells indicated that Y362F and Y370F mutants were non-functional, despite their proper plasma membrane expression. The Y16F and Y363F mutants retained 2.2% and 26% of the wild-type P2X(1) activity, respectively. However, no tyrosine phosphorylation was detected on Western blots of P2X(1) immunoprecipitates derived either from HEK 293 cell lysates or from human platelets, expressing P2X(1) endogenously. Thus, Y16, Y362, Y363 and Y370 are required for the appropriate three-dimensional structure and function of the intracellular P2X(1) domains. [less ▲]

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See detailIntracellular visualization of BrdU-labeled plasmid DNA/cationic liposome complexes.
El Ouahabi, A.; Thiry, Marc ULg; Schiffmann, S. et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1999), 47(9), 1159-66

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA ... [more ▼]

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA complexed with a cationic lipid, Vectamidine (3-tetradecylamino-N-tert-butyl-N'-tetradecylpropionamidine) in BHK21 cells. To facilitate its detection inside the cells, bromodeoxyuridine (BrdU) was incorporated into plasmid DNA under conditions that minimize plasmid alteration. BrdU was localized in cells incubated with Vectamidine/BrdU-labeled plasmid DNA complexes by immunogold labeling and electron microscopy (EM). Labeling was predominantly associated with aggregated liposome structures at the surface of and inside the cells. EM observations of cells transfected with Vectamidine/DNA complexes showed that the liposome/DNA aggregates accumulate in large vesicles in the cell cytosol. On the other hand, using rhodamine-labeled Vectamidine and revealing BrdU with FITC-conjugated antibodies permitted simultaneous detection in the cells of both components of the complexes with confocal laser scanning microscopy. The DNA and lipids co-localized at the surface of and inside the cells, indicating that the complex is internalized as a whole. Our results show that the BrdU-labeled plasmid DNA detection system can be a useful tool to visualize exogenous DNA entry into cells by a combination of electron and confocal microscopy. [less ▲]

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See detailIntraconzole versus amphotericin B plus nystatin in the prophylaxis of fungal infections in neutropenic cancer patients
Boogaerts, Marc; Maertens, Johan; van Hoof, Achiel et al

in Journal of Antimicrobial Chemotherapy (2001), 48

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See detailIntracranial hypotension with subdural hematoma following lumbar puncture: case report
Trunet, S; Litré, Claude Fabien ULg; Tran, H et al

in Neuro-Chirurgie (2008), 54(2), 858

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See detailIntracranial pressure during liver transplantation for fulminant hepatic failure.
Detry, Olivier ULg; Arkadopoulos, N.; Ting, P. et al

in Transplantation (1999), 67(5), 767-70

During orthotopic liver transplantation (OLT) for fulminant hepatic failure (FHF), some patients develop cerebral injury secondary to intracranial hypertension. We monitored intracranial pressure (ICP ... [more ▼]

During orthotopic liver transplantation (OLT) for fulminant hepatic failure (FHF), some patients develop cerebral injury secondary to intracranial hypertension. We monitored intracranial pressure (ICP) and cerebral perfusion pressure (CPP) before and during OLT in 12 FHF patients undergoing transplantation. All four patients who had normal ICP preoperatively maintained normal ICP/CPP throughout OLT. During OLT, four of the eight patients with pretransplant intracranial hypertension had six episodes of ICP increase. These episodes of intracranial hypertension occurred during failing liver dissection (n=3) and graft reperfusion (n=3). At the end of the anhepatic phase, the ICP was lower than the preoperative ICP in all patients, and was below 15 mmHg in all but one patient. These data suggest that in FHF patients who develop intracranial hypertension before OLT, dissection of the native liver and graft reperfusion are associated with a risk of brain injury resulting from intracranial hypertension and cerebral hypoperfusion. [less ▲]

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See detailIntracranial subdural hematoma following spinal anesthesia: case report and review of the literature
Machurot, P. Y.; Vergnion, M.; Fraipont, V. et al

in Acta Anaesthesiologica Belgica (2010), 61(2), 63-66

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See detailIntracranial teratomas in children: the role and timing of surgical removal
Noudel, R; Vinchon, M; Dhellemmes, P et al

in Journal of Neurosurgery (2008), 2(5), 331-8

In this study, the authors report their experience with the surgical treatment of intracranial teratomas with an emphasis on the indications for delayed resection after oncological treatment. Methods. The ... [more ▼]

In this study, the authors report their experience with the surgical treatment of intracranial teratomas with an emphasis on the indications for delayed resection after oncological treatment. Methods. The authors retrospectively reviewed the cases of 14 children with intracranial teratomas. The mean age at diagnosis was 10.5 years (range 2 days–18 years), and 11 patients were male. The final histological analysis revealed pure mature teratoma in 5 cases, mixed teratoma with germinoma in 3 cases, and nongerminomatous malignant germ cell tumor in 6 cases. Thirteen patients underwent tumor resection, and these patients were divided into 2 subgroups according to the timing of surgery. In Group A, 10 patients underwent resection as the primary treatment because no tumor markers were detected in 4 patients, a teratomatous component was revealed on biopsy sampling in 3 patients, and a large tumor volume in 3 patients. In Group B, 3 patients underwent removal of residual pure mature teratoma after oncological treatment. Results. Seven of the 8 patients (87.5%) with pure mature teratomas or with mixed teratoma and germinoma are currently alive (mean follow-up of 9 years); the eighth patient died of postoperative meningitis. Two of the 6 patients (33%) with mixed nongerminomatous malignant germ cell tumors died of tumor progression regardless of the timing of surgery. Conclusions. The results of this study support the belief that microsurgical removal is the only effective treatment for intracranial teratomas. Surgery may be performed as the primary therapy when there is evidence of a noninvasive teratoma, and as a secondary therapy if there is only a partial response to neoadjuvant [less ▲]

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See detailIntradermal immune response after infection with Vaccinia virus.
Jacobs, Nathalie ULg; Chen, Ron A-J; Gubser, Caroline et al

in Journal of General Virology (The) (2006), 87(Pt 5), 1157-61

Although Vaccinia virus (VACV) was used to eradicate smallpox by dermal vaccination, there is little information available about the immune response induced at the vaccination site. Previously, an ... [more ▼]

Although Vaccinia virus (VACV) was used to eradicate smallpox by dermal vaccination, there is little information available about the immune response induced at the vaccination site. Previously, an intradermal murine model that mimics smallpox vaccination was established. Here, this model was used to investigate which leukocytes are recruited to the infected lesion and what are the kinetics of recruitment. Data presented show that VACV infection induced the infiltration of macrophages, followed by granulocytes and lymphocytes. Up to 4 days post-infection, the major lymphocyte population was TCRgammadelta T cells, but thereafter, there was a large recruitment of CD4(+) and CD8(+) T cells. Interestingly, the majority of T cells expressed the natural killer-cell marker DX5. This report is the first to characterize the local immune response sequence to VACV infection and represents a benchmark against which the responses induced by genetically modified VACVs may be compared. [less ▲]

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See detailIntrafollicular LH administration in dairy heifers treated with a GnRH agonist
Mala, J; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Veterinarni Medicina (2013), 58(2), 8186

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to ... [more ▼]

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 μg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10 and 5 μg LH. After IFT using 1 μg of LH 75% animals (3/4) ovulated. The dose of 0.01 μg was not followed by any ovulation whereas control treatments with hCG were followed by an ovulation of the majority of follicles present in the ovaries. In conclusion, IFT with different doses of LH (greater than 0.01 μg) is capable of inducing ovulation. [less ▲]

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See detailIntragrain pinning strength depth dependence of 2223-(Bi,Pb)-based high critical Tc ceramics made by a vitreous route
Dang, An; Godelaine, P. A.; Vanderbemden, Philippe ULg et al

in Journal of Applied Physics (1995), 77(7), 3560-3562

Campbell's method for measuring the critical current in superconductors has been used to obtain the critical current density and the pinning strength in Bi1.7Pb0.3Sr2Ca2Cu3O10−y ceramics synthesized by a ... [more ▼]

Campbell's method for measuring the critical current in superconductors has been used to obtain the critical current density and the pinning strength in Bi1.7Pb0.3Sr2Ca2Cu3O10−y ceramics synthesized by a vitreous route. The intragrain critical current is much higher than 10^5 A/cm2 at 40 K in zero dc magnetic field. A large increase of the pinning strength is observed near the grain surface. The decrease with depth is hyperbolic. The role of the precursors in the synthesis route is emphasized for introducing specific pinning centers. The analysis takes into consideration the ceramics granular nature, i.e., the existence of intergrain and intragrain currents. ©1995 American Institute of Physics. [less ▲]

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See detailIntragranular and intergranular behaviour of multifilamentary Bi-2223/Ag tapes
Fagnard, Jean-François ULg; Vanderbemden, Philippe ULg; Crate, David et al

in Physica C: Superconductivity (2002), 372(Part 2), 970-973

This communication aims at reporting the superconducting properties of Bi-2223/Ag tapes determined by using various measuring techniques. First. the original samples have been characterized by electrical ... [more ▼]

This communication aims at reporting the superconducting properties of Bi-2223/Ag tapes determined by using various measuring techniques. First. the original samples have been characterized by electrical resistance, AC susceptibility. and DC magnetization. The transport (intergranular) critical Current vs. magnetic field was also determined at T = 77 K using pulsed currents up to 40 A. Next. the same combination of experiments as performed oil bent tapes in order to bring out relevant information concerning the strength of the intergranular coupling. The results show that intergranular and intragranular currents differ by at least one order of magnitude, Finally, additional magnetic measurements were carried out in order to determine the anisotropy ratio Jc(ab)/Jc(c), which was found to lie around 30. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailIntragranular and intergranular superconducting properties of bulk melt-textured YBCO
Vanderbemden, Philippe ULg; Cloots, Rudi ULg; Ausloos, Marcel ULg et al

in IEEE Transactions on Applied Superconductivity (1999), 9(2), 2308-2311

We have investigated the superconducting properties of bulk melt-processed YBCO using different experimental techniques. First, single domain materials (i.e. containing no grain boundaries) have been ... [more ▼]

We have investigated the superconducting properties of bulk melt-processed YBCO using different experimental techniques. First, single domain materials (i.e. containing no grain boundaries) have been studied. Electrical resistivity, current-voltage characteristics, M-H loops, and A,C. susceptibility. have been measured for different field and current directions. The intragranular properties are characteristic of high-quality melt-processed YBCO material,,vith a critical current density J(c)(T=77K, B=1T//c) > 10(4) A/cm(2). The anisotropy ratio J(c)(ab)/ J(c)(c) is found to be close to 3. Both magnetic and transport measurements show that defects in the microstructure do not significantly impede the current flow inside the single domain. These results were compared to those measured on samples containing one "natural" single grain boundary which sometimes appears during the grain growth process. The intergranular properties show a much stronger current and magnetic field dependence than that measured within the grain and these differences are discussed. [less ▲]

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See detailIntraguild interactions implicating invasive species: Harmonia axyridis as a model species
Alhmedi, Ammar; Haubruge, Eric ULg; Francis, Frédéric ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14

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