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See detailIn vitro pharmacological activity of salbutamol acetonide on the isolated guinea-pig trachea and porcine bronchus
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, F. et al

in Fundamental & Clinical Pharmacology (2005), 19

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See detailIn vitro pre-digestion to estimate fermentability of feedstuffs in pig large intestine
Boudry, Christelle ULg; Estrada, Fernando; Schoeling, Olivia et al

in Ball, Ronald O. (Ed.) Proceedings of the 9th International Symposium on Digestive Physiology in Pigs (2003, May)

The aim of this study was to compare ileal and faecal digestibilities to gas production in syringes, preceded or not by an in vitro enzymatic digestion of two pig diets containing different amounts of non ... [more ▼]

The aim of this study was to compare ileal and faecal digestibilities to gas production in syringes, preceded or not by an in vitro enzymatic digestion of two pig diets containing different amounts of non starch polysaccharides (NSP). In vivo, only nitrogen faecal digestibility differed between the two diets. Their enzymatic pre-digestion led to a higher gas production after 16 h of incubation in syringes for the diet with the lowest N faecel digestibility but not without pre-digestion. We may conclude that an in vitro pre-digestion before incubation in syringes is necessary to evaluate feed fermentescibility in pig large intestine. [less ▲]

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See detailIn Vitro Preservation of Yam ( Dioscorea cayenensis-D.rotundata complex) for a Better Use of Genetic Resources
Ondo Ovono, Paul ULg; Kevers, Claire ULg; Dommes, Jacques ULg

in Notulae Botanicae Horti Agrobotanici Cluj-Napoca (2010), 38

Among the food crops, yam takes up quantitatively the first place in the gabonese diet. Unfortunately, it can stay available only 6 to 7 months in the year because of difficulties of harvest and post ... [more ▼]

Among the food crops, yam takes up quantitatively the first place in the gabonese diet. Unfortunately, it can stay available only 6 to 7 months in the year because of difficulties of harvest and post- harvest. This problem is little studied in the case of Dioscorea cayenensis-D. rotundata complex. In order to optimize the use of micro tubers for the growing in green house or field, it is important to control the duration of storage before the germination. The present study concerns microtubers obtained by in vitro culture. When microtubers were harvested (after 9 months of culture) and directly transferred on a new medium without hormone, the tubers rapidly sprouted in in vitro conditions. Harvested microtubers were also stored dry in jars in sterile conditions during 2 to 18 weeks before in vitro sprouting. In this case, microtubers stored during 18 weeks sprouted more rapidly than those stored 8 weeks. The size of the tubers used for the storage had great influence on further sprouting. The upper microtubers in 25 mm can be kept to the darkness, under 50% of relative humidity, in 25°C during at least 18 weeks. Sprouting is 100% whatever the substrate of culture. The plant tissue culture technique constitutes a serious alternative for the preservation of plant kinds and for the production of planting material. These techniques allow multiplying in a short time of thousands of copies of new varieties of newly created plants. These in vitro plants can be used on one hand, for the production planting material, and on the other hand for ex vitro storage of breeding grounds with decelerated growth, to struggle against genetic erosion. These results should allow improving in practice the multiplication of yam, while guaranteeing phytosanitary qualities. [less ▲]

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See detailIn vitro production of adaline and coccinelline, two defensive alkaloids from ladybird beetles (Coleoptera : Coccinellidae)
Laurent, Pascal ULg; Braekman, Jean-Claude; Daloze, Désiré et al

in Insect Biochemistry and Molecular Biology (2002), 32(9), 1017-1023

In vitro experiments using [1-C-14] and [2-C-14] acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7 ... [more ▼]

In vitro experiments using [1-C-14] and [2-C-14] acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7-punctata, respectively. The labelled alkaloids obtained in these experiments had a specific activity about ten times higher than that of the samples obtained in feeding experiments. This in vitro assay has enabled us to demonstrate that these two alkaloids are most likely biosynthesised through a fatty acid rather than a polyketide pathway, that glutamine is the preferred source of the nitrogen atom and that alkaloid biosynthesis takes place in the insect fat body. [less ▲]

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See detailIn vitro promotion of root formation by apple shoots through darkness effect on endogenous phenols and peroxidases.
Druart, P.; Kevers, Claire ULg; Boxus, P. et al

in Zeitschrift fur Pflanzenphysiologie (1982), 108(5),

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See detailIn Vitro Propagated Dendritic Cells from Patients with Human-Papilloma Virus-Associated Preneoplastic Lesions of the Uterine Cervix: Use of Flt3 Ligand
Hubert, Pascale ULg; Greimers, Roland ULg; Franzen-Detrooz, E. et al

in Cancer Immunology, Immunotherapy (1998), 47(2), 81-9

Dendritic cells (DC) are the most efficient antigen presenting cells. The clinical use of DC as vectors for antitumor and anti-infectious disease immunotherapy has been limited by their low level and ... [more ▼]

Dendritic cells (DC) are the most efficient antigen presenting cells. The clinical use of DC as vectors for antitumor and anti-infectious disease immunotherapy has been limited by their low level and accessibility in normal tissue. Substantial numbers of DC can be generated from peripheral blood cultured in the presence of interleukin-4 (IL-4) and granulocyte/macrophage-colony-stimulating factor (GM-CSF). We showed in this study that substantial numbers of DC can be obtained from the peripheral blood of patients with (pre)neoplastic lesions of the uterine cervix. The procedure required relatively small blood samples (10 ml) and the presence of 100 U/ml IL-4 and 800 U/ml GM-CSF in the culture medium. There was no significant difference in the morphology, yield, phenotype and function of generated DC between patients with cervical (pre)neoplastic lesions and healthy individuals. When the hematopoietic factor Flt3 ligand (Flt3L, 40 ng;ml) was added, there was an average increase in the DC population of 26% compared to cultures with GM-CSF and IL-4 alone. Approximately 1.2 x 10(6) cells with the characteristics of dendritic cells could be obtained when Flt3L was included in the medium. The addition of Flt3L did not modify the phenotypic profile of DC (HLA-DR+, CD1a+, CD4+, CD54+, CD80+, CD86+. CD40+, CD3- and CD14-). In addition, Flt3L generated functional DC capable of stimulating the proliferation of alloreactive T cells. These results suggest that Flt3L, in association with GM-CSF and IL-4, provides an advantageous tool for the large-scale generation of DC and that an immunotherapy based on the use of DC generated in vitro is possible in patients with (pre)neoplastic lesions of the uterine cervix. [less ▲]

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See detailIn vitro reconstruction of epidermis from primary Darier's disease keratinocytes replicates the histopathological phenotype
LAMBERT DE ROUVROIT, CATHERINE; CHARLIER, CELINE; LEDERER, DAMIEN et al

in Journal of Dermatological Science (2013)

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See detailIn vitro recovery of ATP-sensitive potassium channels in Beta-cells from patients with hyperinsulinism in infancy; effects of low temperature and BPDZ 154
Cosgrove, K. E.; Gonzalez, A. M.; Lee, A. T. et al

in Journal of Physiology (2002), 544

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See detailIn vitro release of salbutamol acetonide from solid lipid nanoparticles
Jaspart, Séverine ULg; Bodson, Cédric; Bertholet, Pascal et al

in Proceedings of 1st Pharmaceutical Sciences Fair and Exhibition (2005)

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See detailIn vitro root cultures of Panax ginseng and P-quinquefolium
Kevers, Claire ULg; Jacques, Philippe; Thonart, Philippe ULg et al

in Plant Growth Regulation (1999), 27(3), 173-178

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took ... [more ▼]

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots. [less ▲]

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See detailIn vitro safety assessment of inhaled products using respiratory epithelial cells
Forbes, Ben; Cao Minh, Quin An; Evrard, Brigitte ULg et al

Poster (2010)

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See detailIn vitro screening of some Strychnos species for antiplasmodial activity
Philippe, Geneviève ULg; Angenot, Luc ULg; De Mol, Patrick ULg et al

in Journal of Ethnopharmacology (2005), 97(3), 535-539

The antiplasmodial activity of crude extracts of 19 species of Strychnos (Loganiaceae) was assessed in vitro against a chloroquine-susceptible strain of Plasmodium falciparum. For each species, ethyl ... [more ▼]

The antiplasmodial activity of crude extracts of 19 species of Strychnos (Loganiaceae) was assessed in vitro against a chloroquine-susceptible strain of Plasmodium falciparum. For each species, ethyl acetate (EtOAc) extracts were analysed and, for the most active species, methanolic (MeOH) extracts were also tested. Among them, Strychnos variabilis De Wild. seemed to be very promising (inhibitory concentration 50% (IC50) < 5 microg/ml) whereas two other species, Strychnos gossweileri Exell and Strychnos mellodora S. Moore, could be interesting (IC50 < 15 microg/ml) in further antimalarial studies. [less ▲]

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See detailIn vitro selection and characterization of DNA aptamers recognizing chloramphenicol
Mehta, Jaytry; Van Dorst, Bieke; Rouah-Martin, Elsa et al

in Journal of Biotechnology (2011), 155

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See detailIn vitro selection of Phytophthora citrophthora isolates resistant to phosphorous acid and fosetyl-Al.
Ali, M. K.; Lepoivre, Philippe ULg; Semal, J.

in Mededelingen van de Faculteit Landbouwwetenschappen (Rijksuniversiteit te Gent) (1988), 53(2b),

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See detailIn vitro simulation of oxic/suboxic diagenesis in an estuarine fluid mud subjected to redox oscillations
Abril, Gwenael; Commarieu, Marc-Vincent ULg; Etcheber, Henri et al

in Estuarine Coastal And Shelf Science (2010), 88(2), 279-291

Estuarine turbidity maxima (ETMs) are sites of intense mineralisation of land-derived particulate organic matter (OM), which occurs under oxic/suboxic oscillating conditions owing to repetitive ... [more ▼]

Estuarine turbidity maxima (ETMs) are sites of intense mineralisation of land-derived particulate organic matter (OM), which occurs under oxic/suboxic oscillating conditions owing to repetitive sedimentation and resuspension cycles at tidal and neap-spring time scales. To investigate the biogeochemical processes involved in OM mineralisation in ETMs, an experimental set up was developed to simulate in vitro oxic/anoxic oscillations in turbid waters and to follow the short timescale changes in oxygen, carbon, nitrogen, and manganese concentration and speciation. We present here the results of a 27-day experiment (three oxic periods and two anoxic periods) with an estuarine fluid mud from the Gironde estuary. Time courses of chemical species throughout the experiment evidenced the occurrence of four distinct characteristic periods with very different properties. Steady oxic conditions were characterised by oxygen consumption rates between 10 and 40 mu mol L-1 h(-1), dissolved inorganic carbon (DIC) production of 9-12 mu mol L-1 h(-1), very low NE4+ and Mn2+ concentrations, and constant NO3 production rates (0.4 - 0.7 mu mol L-1 h(-1)) due to coupled ammonification and nitrification. The beginning of anoxic periods (24 h following oxic to anoxic switches) showed DIC production rates of 2.5-8.6 mu mol L-1 h(-1) and very fast NO consumption (5.6-6.3 mu mol L-1 h(-1)) and NH4+ production (1.4-1.5 mu mol L-1 h(-1)). The latter rates were positively correlated to NO concentration and were apparently caused by the predominance of denitrification and dissimilatory nitrate reduction to ammonia. Steady anoxic periods were characterised by constant and low NO3- concentrations and DIG and NH4+ productions of less than 1.3 and 0.1 mu mol L-1 h(-1), respectively. Mn2+ and CH4 were produced at constant rates (respectively 0.3 and 0.015 mu mol L-1 h(-1)) throughout the whole anoxic periods and in the presence of nitrate. Finally, reoxidation periods (24-36 h following anoxic to oxic switches) showed rapid NH4+ and Mn2+ decreases to zero (1.6 and 0.8-2 mu mol L-1 h(-1), respectively) and very fast NO production (3 mu mol L-1 h(-1)). This NO3- production, together with marked transient peaks of dissolved organic carbon a few hours after anoxic to oxic switches, suggested that particulate OM mineralisation was enhanced during these transient reoxidation periods. An analysis based on C and N mass balance suggested that redox oscillation on short time scales (day to week) enhanced OM mineralisation relative to both steady oxic and steady anoxic conditions, making ETMs efficient biogeochemical reactors for the mineralisation of refractory terrestrial OM at the land-sea interface. (C) 2010 Elsevier Ltd. All rights reserved. [less ▲]

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See detailIn vitro stability and immunoreactivity of the native and recombinant plant food 2S albumins Ber e 1 and SFA-8
Murtagh, M.; Archer, D.; Dumoulin, Mireille ULg et al

in Clinical & Experimental Allergy : Journal of the British Society for Allergy & Clinical Immunology (2003), 8

Background The ability of an intact protein to reach the circulatory system may be a prerequisite to allergenicity and many allergens, particularly those from plant foods, have been found to be ... [more ▼]

Background The ability of an intact protein to reach the circulatory system may be a prerequisite to allergenicity and many allergens, particularly those from plant foods, have been found to be consistently more resistant to digestion by pepsin than other proteins. Objective This study assessed the pepsinolytic stability of native 2S albumins from Brazil nut and sunflower seed and their recombinant versions produced in Pichia pastoris. The physicochemical stability of native and recombinant Brazil nut 2S albumins and recombinant sunflower seed 2S albumin was also assessed. The immunoreactivity of native Brazil nut 2S albumin and recombinant 2S albumins was compared using serum from patients allergic to Brazil nuts and animals immunized with native 2S albumins. Methods Digestibility was measured in simulated gastric fluid followed by SDS-PAGE. Circular dichroism spectra were used to analyse unfolding, as proteins were denatured by temperature, pH and guanidinium chloride. Immunoreactivity was assessed by immunoblot, RAST and ELISA. Results Brazil nut 2S albumin was significantly more resistant to proteolytic digestion than other Brazil nut proteins. It was also resistant to thermally and chemically induced denaturation. Equally high resistance to proteolytic digestion was observed with sunflower seed 2S albumin. The recombinant albumins mirrored their native counterparts in stability and immunoreactivity. Conclusion The important food allergen Brazil nut 2S albumin is as stable to digestion as is sunflower seed 2S albumin, whose allergenicity has yet to be determined. The 2S albumins and their recombinant counterparts could not be easily denatured by physicochemical treatments. The results suggest that 2S albumin is the only Brazil nut protein to reach the gut immune system intact. The production of properly folded recombinant proteins will facilitate mechanistic studies as well as diagnostic testing and antigen-based therapies. [less ▲]

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