Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailImmunogold Labelling of Fatty Acyl Chains
Compère, Philippe ULg; Maneta-Peyret, Lilly; Goffinet, Gerhard ULg et al

in Journal of Immunological Methods (1995), 181(2), 201-9

For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either ... [more ▼]

For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide. [less ▲]

Detailed reference viewed: 11 (3 ULg)
Full Text
Peer Reviewed
See detailImmunogold Silver Staining Associated With Epi-Fluorescence For Cucumber Mosaic Virus Localisation On Semi-Thin Sections Of Banana Tissues
Helliot, B.; Panis, B.; Busogoro, Jp. et al

in European Journal of Histochemistry (2007), 51(2),

Detailed reference viewed: 15 (1 ULg)
Peer Reviewed
See detailImmunogold staining of pituitary hormones in human adenoma biopsies
courtoy, r; Beckers, Albert ULg; Stevenaert, Achille ULg et al

in Biology of the Cell (1985)

Detailed reference viewed: 2 (0 ULg)
Peer Reviewed
See detailImmunogold staining of pituitary hormones in human adenoma biopsies.
Beckers, Albert ULg; Courtoy, R.; Reznik, Michel ULg et al

in Biology of the Cell (1985)

Detailed reference viewed: 5 (1 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical aid at risk stratification of melanocytic neoplasms.
Quatresooz, Pascale ULg; Arrese Estrada, Jorge ULg; Pierard, Claudine ULg et al

in International Journal of Oncology (2004), 24(1), 211-6

There is increasing awareness of the population about the risk of cutaneous melanoma. In recent years, some improvement was gained in the early recognition of clinical warning signs using dermoscopy. As a ... [more ▼]

There is increasing awareness of the population about the risk of cutaneous melanoma. In recent years, some improvement was gained in the early recognition of clinical warning signs using dermoscopy. As a result, the number of puzzling cases exhibiting limited classical clues for malignancy are submitted to the dermatopathologist. Thus, the risk of microscopic uncertainty or misdiagnosis may be increasing. The aim of the study was to assess retrospectively the contribution of immunohistochemistry in establishing the histological diagnosis of 520 melanocytic neoplasms. According to the disease evolution and the histological presentation, 6 profiles of phenotypical expressions were distinguished. [less ▲]

Detailed reference viewed: 19 (7 ULg)
Peer Reviewed
See detailImmunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: a new quantitative method.
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Wyns, Christine et al

in Fertility and Sterility (1994), 62(4), 751-9

Objective: To evaluate estrogen receptors (ER) and progesterone receptors (PR) content in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: A recently advanced stereographic computer ... [more ▼]

Objective: To evaluate estrogen receptors (ER) and progesterone receptors (PR) content in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: A recently advanced stereographic computer technology was applied for the investigation of steroid receptors. SETTING: University hospital department of gynecology. PATIENTS: Biopsies of endometrium and typical peritoneal endometriotic lesions were taken from 19 infertile patients with laparoscopically proved endometriosis. Endometrial biopsies were also taken from 15 patients without endometriosis. All of them were untreated. RESULTS: In normal endometrium, the highest concentrations of ER and PR occurred in the epithelial and stromal cells during the late proliferative phase of the menstrual cycle. Estrogen receptor and PR content declined throughout the secretory phase. Progesterone receptor content was found not to be significantly decreased in the stroma during the early secretory phase and quite high in the late secretory phase. In peritoneal endometriotic lesions, the highest concentrations of ER and PR were found during the late proliferative phase. When compared with normal endometrium, a lower ER content ans a similar PR content were observed, and the cyclic changes in peritoneal endometriosis lesions were also similar. CONCLUSION: A new computerized technology for the evaluation of ER and PR in eutopic and ectopic endometrium. Although the ER content was found to be lower in endometriotic tissue when compared with endometrium, the cyclic pattern was similar in both eutopic and ectopic endometrium. Progesterone receptor content was similar in both tissues, except during the late secretory phase in ectopic glandular epithelium in which a high persistent PR content was observed. [less ▲]

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Donnez, Jacques

in Fertility and Sterility (1997), 68(5), 912-919

Objective: To assess the proliferative activity of eutopic and ectopic endometrium throughout the menstrual cycle and its correlation to steroid receptor content. Design: The immunohistochemical use of Ki ... [more ▼]

Objective: To assess the proliferative activity of eutopic and ectopic endometrium throughout the menstrual cycle and its correlation to steroid receptor content. Design: The immunohistochemical use of Ki 67 was applied to investigate the proliferation index. A recently advanced stereographic computer technology was used to investigate steroid receptors. Setting: University hospital department of gynecology. Patient(s): Biopsies of eutopic endometrium, black and red peritoneal endometriotic lesions, and ovarian endometriomas were taken from infertile patients and classified according to the phase of the cycle. Result(s): In normal endometrium, the glandular proliferation index was highest during the proliferative phase and was statistically significantly reduced during the secretory phase. No proliferative activity was observed in the late secretory phase. No statistically significant differences were found between ectopic endometrium and eutopic endometrium except during the late secretory phase, when proliferative activity was still present in endometriotic tissue. The stromal proliferation index was similar in red lesions, ovarian endometriomas, and eutopic endometrium during the secretory phase. In normal endometrium, the highest concentrations of estrogen receptors (ERs) and P receptors (PRs) occurred in the epithelial and stromal cells during the late proliferative phase of the menstrual cycle. Estrogen receptor and PR content declined throughout the secretory phase. In ectopic endometrium, PR persisted in the glandular epithelium during the late secretory phase. Estrogen receptors persisted in the glandular epithelium and stroma of red peritoneal lesions and ovarian endometriomas during the late secretory phase. Conclusion(s): The high proliferative activity and the persistence of ERs and PRs in the stroma of red lesions and ovarian endometriomas emphasize the primordial role of the stroma in the development of endometriosis and suggest different mechanisms of proliferation control from those observed in eutopic endometrium. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailIMMUNOHISTOCHEMICAL ANALYSIS OF TRANSFORMING GROWTH FACTOR BETA 1 AND ITS SIGNALLING PATHWAYS IN CANINE IDIOPATHIC PULMONARY FIBROSIS
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

Detailed reference viewed: 14 (4 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical Aspects of the Fibrogenic Pathway in Nephrogenic Systemic Fibrosis.
Quatresooz, Pascale ULg; Paquet, Philippe ULg; Hermanns-Lê, Trinh ULg et al

in Applied Immunohistochemistry & Molecular Morphology (2010)

Nephrogenic systemic fibrosis (NSF) is a rare gadolinium-dependent disorder of the skin and viscera. The aim of this study was to revisit some immunopathologic clues of NSF, including the characterization ... [more ▼]

Nephrogenic systemic fibrosis (NSF) is a rare gadolinium-dependent disorder of the skin and viscera. The aim of this study was to revisit some immunopathologic clues of NSF, including the characterization of glycosaminoglycans, cell tensegrity, and cell proliferation in the dermis. Immunohistochemistry was done using antibodies directed to vimentin, CD34, Factor XIIIa, calprotectin, alpha-smooth muscle actin, Ulex europaeus agglutinin-1 (UEA-1), and MIB1/Ki67 and to glycosaminoglycans, including CD44 var3, versican, and perlecan. The vimentin+ cell density was markedly increased. The vast majority of them corresponded to CD34+ or Factor XIIIa+ dermal dendrocytes (DD) showing distinct cell tensegrity. CD34+DD were slender, elongated, and usually scattered in the dermis but focally clustered in nodular collections. By contrast, Factor XIIIa+ was plump with squat dendrites showing no evidence for being under mechanical stress. Cells in the vicinity of the microvasculature were rounded and exhibited calprotectin immunoreactivity typical for monocyte/macrophages. The microvasculature highlighted by UEA-1 and alpha-smooth muscle actin looked unremarkable. The cell proliferation highlighted by the MIB/Ki67 immunoreactivity was unusually high (>20%) in the interstitial stromal cells. Stromal cells enriched in versican were plump, abundant, and seemed interconnected each other by a dense network of dendrites. By contrast, the immunolabeling for perlecan and CD44 var 3 was unremarkable. In conclusion, the cell population involved in NSF seemed phenotypically heterogeneous, and its growth fraction was clearly boosted in the skin. The intracellular load in versican was prominent. The aspect of cell tensegrity did not suggest the influence of mechanical stress putting stromal cells under tension in the dermis. [less ▲]

Detailed reference viewed: 12 (3 ULg)
Peer Reviewed
See detailImmunohistochemical characterisation of the inflammatory infiltrate associated with canine nasal carcinoma.
Vanherberghen, Morgane ULg; Day, M. J.; Gabriel, Alexandra et al

in Proceedings of the 17th Annual Congress of the ECVIM-CA (2007)

Detailed reference viewed: 10 (2 ULg)
Peer Reviewed
See detailImmunohistochemical characterization of the basement membranes of the human oral mucosa.
Salonen, J.; Pelliniemi, L. J.; Foidart, Jean-Michel ULg et al

in Archives of Oral Biology (1984), 29(5), 363-8

Type IV and V collagens, laminin and heparan sulphate proteoglycan were localized in vascular and subepithelial basement membranes. Fibronectin was distributed in a reticular pattern throughout the lamina ... [more ▼]

Type IV and V collagens, laminin and heparan sulphate proteoglycan were localized in vascular and subepithelial basement membranes. Fibronectin was distributed in a reticular pattern throughout the lamina propria under the oral epithelium. The uniform distribution of basement membrane components and type V collagen in different regions suggests a similar molecular composition for the basement membranes under functionally-different oral epithelia. The more intense reaction in the vascular than in the subepithelial basement membranes, with diluted antibodies to type IV collagen and laminin apparently reflects chemical differences in these basement membranes. Occasional discontinuities in the subepithelial basement membranes were seen in inflamed gingival sulci and in tonsillar crypts. The destruction responsible affected all basement membrane components, except fibronectin, which maintained a reticular distribution even in the deep tonsillar tissue. The immunohistochemical method is useful in demonstrating different degrees of destruction in basement membranes associated with inflammation. [less ▲]

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical clues at aging of the skin microvascular unit.
Quatresooz, Pascale ULg; Pierard, Gérald ULg

in Journal of Cutaneous Pathology (2009), 36(1), 39-43

BACKGROUND: Skin aging is a multifaceted process influenced by various factors affecting at variable degree at different body sites. This study focused on the aging process affecting dermal dendrocytes ... [more ▼]

BACKGROUND: Skin aging is a multifaceted process influenced by various factors affecting at variable degree at different body sites. This study focused on the aging process affecting dermal dendrocytes and the microvasculature. We also explored the effect of substance P released by capsaicin because this neuropeptide is known to affect the microvasculature environment. METHODS: The first part of the present study was undertaken in two groups of 35 men who were younger than 30 years and older than 65 years, respectively. A skin biopsy was taken from the intact skin of a thigh during orthopedic surgical intervention after road accident. The second part of the study was performed on 30 men aged 65-68 years. They applied once daily a 0.05% capsaicin gel and the vehicle in a randomized design on the volar forearms. A skin biopsy was taken at inclusion and after a 5-month treatment phase. Immunohistochemistry was performed using a panel of antibodies. Dermal cells were distinguished according to the presence of factor XIIIa, CD34 or thrombomodulin. Blood and lymphatic vessels were studied by Ulex europaeus agglutinin-1, human podoplanin, human vascular endothelial growth factor-C (H-VEGF-C) and alpha-actin immunoreactivities. RESULTS: In the study phase 1, a significant decrease in thrombomodulin-positive cells and vascularity were evidenced in the aged group. In the study phase 2, capsaicin appeared to boost the factor XIIIa-positive dendrocytes, the thrombomodulin-positive cells and the blood vessel network as well. CONCLUSIONS: Specific subsets of the dermal dendrocyte populations and the blood microvasculature appear affected by aging. Capsaicin may limit these aging effects. [less ▲]

Detailed reference viewed: 48 (5 ULg)
Peer Reviewed
See detailImmunohistochemical demonstration of laminin, the major glycoprotein of basement membranes, as an aid in the diagnosis of soft tissue tumors.
Miettinen, M.; Foidart, Jean-Michel ULg; Ekblom, P. G.

in American Journal of Clinical Pathology (1983), 79(3), 306-11

Forty-eight benign and malignant soft tissue tumors were investigated immunohistologically for the presence of laminin, a glycoprotein of basement membranes. The results showed intense laminin positivity ... [more ▼]

Forty-eight benign and malignant soft tissue tumors were investigated immunohistologically for the presence of laminin, a glycoprotein of basement membranes. The results showed intense laminin positivity in schwannomas and neurofibromas and less intense positivity in leiomyomas and leiomyosarcomas, whereas fibrous histiocytomas and fibrosarcomas generally were negative. It is suggested that immunohistochemical demonstration of laminin is a valuable aid in the differential diagnosis between tumors derived from fibroblasts and Schwann cells, and a potential aid in the differential diagnosis between tumors originating from fibroblasts and those from smooth muscle cells. In addition to the expression seen in the tumors, all vascular walls were positive for laminin. Therefore, demonstration of laminin also can be used to examine the vascular pattern of tumors. [less ▲]

Detailed reference viewed: 1 (0 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical detection of immediate early and late phase proteins expressed during the varicella-zoster virus cycle
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in British Journal of Dermatology. Supplement (1994), 131(44), 64

Detailed reference viewed: 12 (2 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical detection of incipient melanoma micrometastases. Relationship with sentinel lymph node involvement.
Claessens, Nadine; Pierard, Gérald ULg; Pierard, Claudine ULg et al

in Melanoma Research (2005), 15(2), 107-10

It is acknowledged that tumour thickness, ulceration and lymph node invasion are the most important prognostic factors for cutaneous melanomas. Other histopathological features may also be informative ... [more ▼]

It is acknowledged that tumour thickness, ulceration and lymph node invasion are the most important prognostic factors for cutaneous melanomas. Other histopathological features may also be informative. The aim of this study was to ascertain whether immunohistochemical methods can improve the detection of satellite micrometastases in primary melanoma patients. In addition, the predictive value of cutaneous satellite micrometastases for sentinel lymph node involvement was evaluated. A total of 265 primary cutaneous melanomas and 68 of the respective sentinel nodes were studied using a panel of seven antibodies directed against melanocyte-related antigens. In 12.4% of the 265 cases, small satellite micrometastases were detected by immunohistochemistry. Sentinel lymph node metastases were found in 14% of the 68 cases. Invasion of the sentinel lymph node correlated with the presence of cutaneous satellite micrometastases. It is concluded that the presence of cutaneous satellite micrometastases may be an indication for the performance of sentinel lymph node biopsy, and this finding calls for a closer follow-up of these patients. [less ▲]

Detailed reference viewed: 55 (8 ULg)
Peer Reviewed
See detailImmunohistochemical evidence for cholecystokinin-like peptides in neuronal cell bodies of the rat spinal cord.
Vanderhaeghen, J. J.; Deschepper, C.; Lotstra, F. et al

in Cell & Tissue Research (1982), 223(2), 463-7

Cholecystokinin-like immunoreactivity has been demonstrated by radioimmunoassay and immunocytochemistry in the spinal cord of various mammals, in particular in nerve fibers of the superficial layers of ... [more ▼]

Cholecystokinin-like immunoreactivity has been demonstrated by radioimmunoassay and immunocytochemistry in the spinal cord of various mammals, in particular in nerve fibers of the superficial layers of the posterior column, but had not been detected in neuronal cell bodies. We report immunohistochemical evidence for the presence of a group of cholecystokinin-containing neuronal cell bodies in the lumbar spinal cord of the rat. This group of cells is only visualized after direct injection of colchicine into the spinal cord and is located near the central canal in the intermedio-medial nucleus of area X of Rexed. [less ▲]

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailImmunohistochemical evidence for the presence of various kisspeptin isoforms in the mammalian brain.
Franceschini, I.; Yeo, S. H.; Beltramo, M. et al

in Journal of neuroendocrinology (2013), 25(9), 839-51

Kisspeptins are small peptides encoded by the Kiss1 gene that have been the focus of intense neuroendocrine research during the last decade. Kisspeptin is now considered to have important roles in the ... [more ▼]

Kisspeptins are small peptides encoded by the Kiss1 gene that have been the focus of intense neuroendocrine research during the last decade. Kisspeptin is now considered to have important roles in the regulation of puberty onset and adult oestrogen-dependent feedback mechanisms on gonadotrophin-releasing hormone secretion. Several kisspeptin antibodies have been generated that have enabled an overall view of kisspeptin peptide distribution in the brain of many mammalian species. However, it remains that the distribution of the different kisspeptin isoforms is unclear in the mammalian brain. In the present study, we report on two new N-terminal-directed kisspeptin antibodies, one against the mouse kisspeptin-52 sequence (AC053) and one against the rat kisspeptin-52 sequence (AC067), and use them to specifically map these long isoforms in the brains of mouse and rat, respectively. Kisspeptin-52 immunoreactivity was detected in the two main kisspeptin neuronal populations of the rostral periventricular area and arcuate nucleus but not in the dorsomedial hypothahamus. A large number of fibres throughout the ventral forebrain were also labelled with these two antibodies. Finally, a comparison with the most commonly used C-terminal-directed kisspeptin antibodies further suggests the presence of shorter kisspeptin fragments in the brain with specific inter- and intracellular expression patterns. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailImmunohistochemical expression of epidermal growth factor receptors in nuclei of a subpopulation of keratinocytes and sweat gland cells.
Pierard, Claudine ULg; Colige, Alain ULg; Arrese Estrada, Jorge ULg et al

in Dermatologica (1991), 183(1), 7-9

We have raised a polyclonal antibody to the 170-kD epidermal growth factor receptor. We found an intercellular pattern of immunoreactivity in the epidermis as well as a positivity of the cytoplasm of ... [more ▼]

We have raised a polyclonal antibody to the 170-kD epidermal growth factor receptor. We found an intercellular pattern of immunoreactivity in the epidermis as well as a positivity of the cytoplasm of keratinocytes and eccrine secretory cells. In some samples, a nuclear labelling was evidenced in these type of cells. There is a close resemblance in the topographical distribution of these cells with nuclear labelling and those synthesizing DNA under phytohaemagglutinin stimulation. [less ▲]

Detailed reference viewed: 10 (2 ULg)
Peer Reviewed
See detailImmunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use.
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in Journal of Medical Virology (1995), 47(4), 342-7

Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical ... [more ▼]

Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical and immunohistochemical identification of the VZV ORF63 encoded protein (IE63) and of the VZV late protein gE on smears and formalin-fixed paraffin-embedded skin sections taken from lesions clinically diagnosed as varicella (n = 15) and herpes zoster (n = 51). Microscopic examinations of Tzanck smears and skin sections yielded a diagnostic accuracy of Herpesviridae infections in 66.7% (10/15) and 92.3% (12/13) of varicella, and 74.4% (29/39) and 87.8% (43/49) of herpes zoster, respectively. Immunohistochemistry applied to varicella provided a type-specific virus diagnostic accuracy of 86.7% (13/15; IE63) and 100% (15/15; gE) on smears, and of 92.3% for both VZV proteins on skin sections. In herpes zoster, the diagnostic accuracy of immunohistochemistry reached 92.3% (36/39; IE63) and 94.9% (37/39; gE) on smears, and 91.7% (44/48; IE63) and 91.8% (45/49; gE) on skin sections. These findings indicate that the immunohistochemical detection of IE63 and gE on both smears and skin sections yields a higher specificity and sensitivity than standard microscopic assessments. [less ▲]

Detailed reference viewed: 14 (2 ULg)