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See detailIn vitro study on the effects of aceclofenac on proteoglycan and type II collagen production by human chondrocytes
Henrotin, Yves ULg; Labasse, A; Degroote, D et al

in Osteoarthritis and Cartilage (1997), 5

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See detailIn vitro survival of vitrified goat embryos: comparison of two vitrification methods
Guignot, F.; Baril, G.; Pougnard, J. L. et al

in Proceedings: 17e Réunion A.E.T.E. (2001)

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See detailIn vitro susceptibility of Clostridium perfringens isolated from farm animals to growth-enhancing antibiotics.
Devriese, L. A.; Daube, Georges ULg; Hommez, J. et al

in Journal of Applied Bacteriology (1993), 75

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See detailIn Vitro Susceptibility Testing of Aspergillus fumigatus against Posaconazole: Comparison of NCCLS M38-P and E-Test Methods
Hayette, Marie-Pierre ULg; Amadore, Agatha; Seidel, Laurence ULg et al

Poster (2002, September)

Posaconazole is a second-generation triazole and structural analogue of itraconazole. This drug has fungicidal activity against yeasts and filamentous fungi. The aim of our study was to evaluate E-test ... [more ▼]

Posaconazole is a second-generation triazole and structural analogue of itraconazole. This drug has fungicidal activity against yeasts and filamentous fungi. The aim of our study was to evaluate E-test method for in vitro susceptibility testing of Aspergillus fumigatus isolates against posaconazole. METHODS: A total of 121 isolates of A. fumigatus were selected as follows: 106 clinical strains from colonized patients, 18 from patients with invasive aspergillosis and 7 environmental isolates. Their in vitro susceptibility was evaluated by E-test (Abbiodisk, Sweden) and compared with NCCLS microdilution reference method (M38-P). Both tests were performed with RPMI 1640 medium at 35 degrees C. MIC values were read after 24h (MIC-24h) and 48h (MIC-48h) incubation time by E-test method. Two MIC endpoints were determined by NCCLS method: 1.no visible reduction of growth (MIC-0); 2. 50% reduction (or more) of growth (MIC-2). Three A. fumigatus reference strains (IHEM 5734, 6149 and 13935) were included as control. RESULTS: Geometric mean MICs (microg/ml) were respectively 0.02 for E-test at 24h and 0.029 at 48h. MIC-0 and MIC-2 values were respectively 0.19 and 0.018 microg/ml. One correlation between both methods was observed for MICs-24h and MICs-2s (p<.05). However, there was no significant difference according to origin of isolates (p<.05). CONCLUSIONS: 1. This study assessed the potent role of posaconazole against A. fumigatus isolates with very low MICs. 2. MIC values were not predictive of pathogenicity. 3. E-test method by reading after 24h-incubation time could easily replace the time-consuming NCCLS M38-P reference method. [less ▲]

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See detailIn Vitro Synergy of Polymyxins and Carbapenems: Systematic Review and Meta Analysis.
Zusman, Oren; Avni, Tomer; Leibovici, Leonard et al

in Antimicrobial Agents and Chemotherapy (2013), 57(10), 5104-51011

ObjectivesTo examine the evidence on in-vitro synergy of polymyxin-carbapenem combination therapy against Gram-negative bacteria (GNB)MethodsSystematic review and meta-analysis. All studies examining in ... [more ▼]

ObjectivesTo examine the evidence on in-vitro synergy of polymyxin-carbapenem combination therapy against Gram-negative bacteria (GNB)MethodsSystematic review and meta-analysis. All studies examining in-vitro interactions of antibiotic combinations consisting of any carbapenem with colistin or polymyxin B against any GNB. A broad search was conducted with no language, date or publication status restrictions. Synergy rates, defined as fractional inhibitory concentration index </=0.5 or >2log colony forming unit reduction, were pooled separately for time-kill, checkerboard, and E-test in a mixed-effects meta-analysis of rates. We examined whether synergy rate depended on testing method, type of antibiotic, bacteria and resistance to carbapenems. Pooled rates with 95% confidence intervals are shown.Results39 published studies and 15 conference proceeding were included, reporting on 246 different tests on 1054 bacterial isolates. In time-kill studies, combination therapy showed synergy rates of 77% (95% CI 64-87) for Acinetobacter baumannii, 44% (95% CI 30-59%) for Klebsiella pneumoniae and 50% (95% CI 30-69%) for Pseudomonas aeruginosa with low antagonism rates for all. Doripenem showed high synergy rates for all three bacteria. For A. baumannii, meropenem was more synergistic than imipenem, whereas for P. aeruginosa the opposite was true. Checkerboard and Etest studies generally reported lower synergy rates than time-kill. Use of combination therapy led to less resistance development in-vitro.ConclusionsThe combination of a carbapenem with a polymyxin against GNB, especially A. baumannii, is supported in-vitro by high synergy rates, with low antagonism and less resistance development. These findings should be examined in clinical studies. [less ▲]

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See detailIn vitro tests for evaluation of the hatchability of the eggs of Psoroptes mites following exposure to acaricidal compounds.
Lekimme, Mireille ULg; Mignon, Bernard ULg; Leclipteux, Thierry ULg et al

in Medical and Veterinary Entomology (2006), 20(1), 102-105

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high ... [more ▼]

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high rates when eggs are incubated under conditions of high humidity, on a liquid medium and in agarose dishes. These three protocols were compared, taking into account the ease of preparation, follow-up and accuracy of counting. The best protocol was found to be the use of agarose dishes. It was accurate, easy to carry out and reproducible for further evaluation of existing or potentially new compounds against both adults and eggs of Psoroptes spp. The acaricidal properties of phoxim and amitraz were then evaluated against eggs and adults using the three protocols. Results showed that for both drugs, in vitro adulticidal activity was complete, whereas the in vitro ovicidal activity was only partial. Nevertheless, efficacy of amitraz against both adults and eggs was shown to be higher than that of phoxim. [less ▲]

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See detailIn vitro titration of Theileria parva tick derived stabilates
Marcotty, T.; Speybroeck, N.; Berkvens, D. et al

in Parasitology (2004), 128(Part 2), 131-137

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic ... [more ▼]

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. Parva sporozites used in this method is usually determined by in vitro titration. An alternative in vivo method of quantitification of sporozoites ill whole tick-derived stabilites is proposed. The method consists of incubating serially diluted T. Parva stabilities with boville peripheral blood lymphocytes, the host cell that is infected naturally. Allowing the cultures to incubate undisturbed for the full cultivation period (10 days) reduced the variability amoung replicate titrations. fungal contaminations were avoided by centrifuging stabilates at 400 g prior to the incubation, which did not precipiate sporozoites significantly. Fungistics, Nysatin and Flucytosine did not appear to interfere with the in vitro development of 2 stabilates but their effect on fungal growth was limited. In vitro titration data were compared to in vivo infection data for 2. In vivo titration of T. parva sporozoites should allow more ethicl and efficient research on the preparation and storage of T. Parva tick-derived stabilates. [less ▲]

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See detailIn vitro transport studies of nifedipine nanoparticles across Caco-2/HT29- 5M21 cultures and co-cultures
Hecq, Julien; Nollevaux, Géraldine; Deleers, M. et al

Poster (2006)

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See detailIn vitro tuberization of potato.
Meulemans, M.; Anceau, Christine ULg; Dumont, Josée ULg et al

in Internationaal Symposium over Fytofarmacie en Fytiatrie (1986), 38

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See detailIn vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne, Christophe ULg; Lapiere, C. M.; Nusgens, Betty ULg

in Cardiovascular Research (2001), 49(3), 647-58

OBJECTIVE: This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. METHODS ... [more ▼]

OBJECTIVE: This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. METHODS: The morphotype, biosynthetic phenotype and cytoskeleton organization of human umbilical vein endothelial cells (HUVEC) were analyzed on supports of variable mechanical resistance. RESULTS: Western blot analysis revealed a strong reduction of the expression of actin and focal-adhesion plaque (FAP) proteins in HUVEC organized in tube-like structures (TLS) on soft matrigel or on matrigel co-polymerized with heat-denatured collagen as compared to HUVEC remaining in a monolayer pattern on rigid matrigel-coat or on matrigel co-polymerized with type I collagen. Human skin fibroblasts morphotype was not altered in these culture conditions and the pattern of FAP proteins and actin was not modulated. By using polyacrylamide gels polymerized with various concentrations of bis-acrylamide to modulate the mechanical resistance of the support and cross-linked to a constant amount of gelatin to provide an equal density of attachment sites, it was shown that the less rigid the support, the more endothelial cells switched to a tube-like pattern. Collagen type I-induced tubulogenesis was accompanied by a profound and reversible remodeling of the actin-FAP complex suggesting a weakening of the bridging between extracellular matrix (ECM) and the cytoskeleton. Human skin fibroblasts and smooth muscle cells, used as control cells, adhered strongly to the collagen, did not form TLS and their network of actin stress fibers was not remodeled. The inhibition of collagen type I-induced tubulogenesis by agents altering the actin cytoskeleton-FAP complex including calpain type I inhibitor, orthovanadate, KT5720 and jasplakinolide, further supports the determinant role of mechanical coupling between the cells and the matrix in tubulogenesis. CONCLUSIONS: A reduced tension between the endothelial cells and the extracellular matrix, originating in the support or within the cells is sufficient to trigger an intracellular signaling cascade leading to tubulogenesis, an event mimicking one of the last steps of angiogenesis. [less ▲]

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See detailAn in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
Ceusters, Justine ULg; Serteyn, Didier ULg; MINGUET, Grégory ULg et al

in Veterinary Immunology and Immunopathology (2012), 150(3-4), 221-7

Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate ... [more ▼]

Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate equine neutrophils in whole blood and to evaluate their response by measuring the release of total and active myeloperoxidase (MPO) and total elastase, considered as markers of neutrophil stimulation and degranulation. Because of the critical importance of the concomitant presence of LPS and TNF-alpha in equine pathological situations, we combined these two natural mediators to stimulate PMN and compared the response with those obtained after the PMN stimulation with each mediator used alone and well-known artificial stimulation systems such as 12-phorbol 13-myristate acetate (PMA) and the combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). All the activation systems, PMA, CB/fMLP, TNF-alpha, LPS and LPS/TNF-alpha, induced a significant release of total MPO in whole blood but only the combinations CB/fMLP and LPS/TNF-alpha significantly favored the release of active MPO. Regarding the total elastase, we did not observe a significant release in all the stimulated conditions except with PMA. It appears clearly that the choice of the neutrophil stimulation model is fundamental for the selection of potentially active pharmacological agents, especially on MPO activity. [less ▲]

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See detailIn vitro-generated interspecific recombinants between bovine herpesviruses 1 and 5 show attenuated replication characteristics and establish latency in the natural host
Del Medico Zajac, M. P.; Romera, S. A.; Ladelfa, M. F. et al

in BMC Veterinary Research (2011), 7

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See detailIn Vivo 31p Nuclear Magnetic Resonance Spectroscopy of Skeletal Muscle Energetics in Endotoxemic Rats: A Prospective, Randomized Study
Gilles, R. J.; D'Orio, Vincenzo ULg; Ciancabilla, F. et al

in Critical Care Medicine (1994), 22(3), 499-505

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal ... [more ▼]

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal muscle energetics in endotoxemic and in control rats, by in vivo 31P nuclear magnetic resonance (NMR) spectroscopy at rest, under regional ischemia, and during reperfusion. SETTING: Biochemical NMR laboratory equipped for surgery and hemodynamic monitoring. SUBJECTS: Wistar rats were randomized to different groups. Eight rats were injected with Escherichia coli endotoxin (15 mg/kg, survival time 19 +/- 4 hrs) intraperitoneally. Seven other rats served as controls. The additional nine rats were studied for the saturation recovery pulse sequence. INTERVENTIONS: In the treatment group, endotoxin was injected 8 hrs before NMR spectroscopy. The right hind limbs were studied under anaesthesia using a surface coil NMR probe. Their high-energy phosphate contents and intracellular pH were determined by 31P NMR spectroscopy. After baseline measurements, an ischemia-reperfusion challenge was imposed on the muscle by transient clamping of the abdominal aorta. Contralateral femoral artery pressure was constantly monitored. MEASUREMENTS AND MAIN RESULTS: During the baseline period, the endotoxin-treated muscles did not show any difference in the distribution of the high-energy phosphate compounds or in intracellular pH, as compared with the controls. Ischemia resulted in a significantly faster decline of the inorganic phosphate/creatine phosphate ratio in the endotoxin-treated rats (1.35 +/- 0.17 vs. 0.51 +/- 0.06 at the end of the 38-min ischemic period). Skeletal muscle acidosis developed earlier and was deeper in the endotoxemic animals (pH: 6.94 +/- 0.02 vs. 7.02 +/- 0.03 at the end of ischemia). During reperfusion, the calculated time constants of recovery of inorganic phosphate to phosphocreatine ratios were identical between groups. CONCLUSIONS: Resting nonischemic muscles of endotoxin-treated rats show no evidence of alterations in high-energy phosphate metabolism. However, under ischemic conditions, high-energy phosphate metabolism deteriorates faster in the skeletal muscles of treated animals. These data support the hypothesis of a greater mismatch during perfusion at very low pressure between residual oxygen availability and oxygen needs in the endotoxin-treated muscle cell. [less ▲]

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See detailIn vivo administration of a PKA type I inhibitor (Rp-8-Br-cAMPS) restores T-cell responses in retrovirus-infected mice
Nayjib, Btissam ULg; Zeddou, Mustapha ULg; Drion, Pierre ULg et al

in Open Immunol journal (2008), 1

Murine AIDS (MAIDS) is caused by infection with the murine leukemia retrovirus RadLV-Rs and is characterized by T-cell anergy and severe immunodeficiency with increased susceptibilty to several ... [more ▼]

Murine AIDS (MAIDS) is caused by infection with the murine leukemia retrovirus RadLV-Rs and is characterized by T-cell anergy and severe immunodeficiency with increased susceptibilty to several experimental opportunistic infections as observed in HIV infection. T cell anergy is associated with an increase of intracellular cAMP level, triggering a multistep pathway involving activation of PKA type I and resulting in inhibition of proximal TCR signaling. We have reviously demonstrated that blocking PKA type I using the selective inhibitor Rp-8-Br-cAMPS, restores T-cell function in vitro in MAIDS as well as in HIV infection. In the present report, we investigated the effect of parenteral administration of Rp-8-Br-cAMPS in mice with MAIDS. We show that the compound is not toxic and partially restores the ex vivo proliferative responses to anti-CD3 mAb, but that it has no effect on the lymphadenopathy and splenomegaly characterizing the MAIDS syndrome. [less ▲]

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See detailIn vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate
Péters, Fabrice; Franck, Thierry ULg; Pequito, Manuel et al

in Journal of Veterinary Pharmacology & Therapeutics (2009)

The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by ... [more ▼]

The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by neutrophils was studied in two groups of horses, which received a dose of 0.1 mg⁄ kg of either acepromazine or promethazine intravenously. Blood samples were collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The chemiluminescence (CML) response of neutrophils was measured ex vivo in the presence of luminol for a period of 10 min and the maximum CML value (peak value) recorded. There was a significant inhibition of the ROS production in the acepromazine treated group (49% inhibition) at 5 h after administration and in the promethazine group (24% inhibition) at 3 h after administration (P < 0.05 vs. T0). These findings are of therapeutic relevance in the use of phenothiazines in equine patients with inflammatory diseases where neutrophil activation and ROS production are implicated. [less ▲]

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See detailIn vivo and in vitro development of preantral follicles
Van den Hurk, R.; Bevers, M. M.; Beckers, Jean-François ULg

in Theriogenology (1997), 47(1), 73-82

The paper presents the state of the art concerning the in vitro growth and differentiation of preantral follicles, i.e. from the primordial to the antral stage, in mammals. Attention is paid to their ... [more ▼]

The paper presents the state of the art concerning the in vitro growth and differentiation of preantral follicles, i.e. from the primordial to the antral stage, in mammals. Attention is paid to their normal structure and development, nomenclature, isolation and culture methods, effects of systemic and local compounds on their development, and viability of isolated and cultured follicules, with special reference to the bovine. In vivo, various endocrine and locally produced compounds stimulate neovascularization or innervation of small follicles, which provide nutrients, cytokines, hormones and neuropeptidergic substances, necessary of survival of follicles and initiation of follicle growth. In vitro, FSH, activin, EGF, TGFs and IVP seem important for survival and growth of cultured preantral follicles. However, many reported in vitro effects of hormonal compounds on preantral follicles, especially on those from the bovine, have to be interpreted carefully, since these studies presented little information on oocyte quality. In contrast to preantral follicles from small laboratory animals, pigs and humans, those from cows will not soon be usable for IVP, since their oocyte is relatively small and their growth rate relatively slow. Although practical application of especially small preantral for IVP of live-stock species still is far away, it should not be considered an utopia. [less ▲]

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See detailIn vivo and in vitro effect of acyclovir on pseudorabies virus, infectious bovine rhinotracheitis virus and pigeon herpesvirus.
Thiry, Etienne ULg; Vindevogel, Henri ULg; Leroy, Pascal ULg et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1983), 14(3), 239-45

The effect of various concentrations of acyclovir on the mean plaque size of pseudorabies virus (SHV), infectious bovine rhinotracheitis virus (IBR virus) and pigeon herpesvirus (PHV) has been studied ... [more ▼]

The effect of various concentrations of acyclovir on the mean plaque size of pseudorabies virus (SHV), infectious bovine rhinotracheitis virus (IBR virus) and pigeon herpesvirus (PHV) has been studied. Acyclovir significantly reduced the mean plaque size of SHV and PHV, whereas IBR virus was less affected and did only show a reduction of the mean plaque size at the highest concentration of acyclovir used (1000 microM). In vivo effect of acyclovir was tested using pigeons and budgerigars experimentally infected with PHV and rabbits experimentally infected with a very low dose of SHV. Intramuscular injections of acyclovir (100 mg/kg/day; three injections/day) did not prevent the appearance of clinical disease in infected pigeons nor did reduce the level of viral excretion. The same treatment applied, as for the pigeons, before infection protected most of the budgerigars as long as they were treated, but most of them died soon after the end of the treatment. Only one rabbit was protected by the treatment. SHV was recovered in the lung of only one of the treated animals, whereas it was isolated in the lungs of each control animal. [less ▲]

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See detailIn vivo and in vitro effect of new glibenclamide isosteres
Nguyen, Q. A.; Ouedraogo, R.; Antoine, M. H. et al

Conference (1998, December 12)

Detailed reference viewed: 8 (0 ULg)