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See detailFrom Metabolomics to Identification of a new therapeutic approach for Age-Related Macular Degeneration (AMD)
Schoumacher, Matthieu ULg; De Tullio, Pascal ULg; LAMBERT, Vincent ULg et al

Poster (2016, May)

Age-related macular degeneration (AMD) is the leading cause of vision loss in the western world among people aged 50 or older. 90% of all vision loss due to AMD result from the exudative form, which is ... [more ▼]

Age-related macular degeneration (AMD) is the leading cause of vision loss in the western world among people aged 50 or older. 90% of all vision loss due to AMD result from the exudative form, which is characterized by choroidal neovascularization (CNV). Age-related changes that induce pathologic CNV are incompletely understood and critical issues remain to be addressed. Metabolomics is defined as the comprehensive study of endogenous metabolites changes in various biological systems. This newly emerging “omic” science provides a unique opportunity to correlate variation of the metabolome with pathological occurrence or progression and/or to identify metabolites that are implicated in the disease. We apply a 1H NMR metabolomics approach on sera collected from AMD patient and healthy volunteers and form a mice model of laser-induced CNV which mimics the effect of exudative AMD. After post-processing treatments, the different spectra were analyzed by statistical discriminant methodologies (PCA, ICA, PLS-DA, O-PLS-DA). These approaches allow the differentiation between control and AMD patients and between laser-induced mice and the control mice group. Moreover, the same discriminating spectral zones have been identified in human and mice model, leading to the emergence of different putative biomarkers. Among these markers, lactate emerges as a key metabolite in both settings. Mechanistically, lactate produced locally and by inflammatory cells, plays a critical role in the onset of the inflammatory and angiogenic phases. In mice model of laser-induced CNV, normalization of circulating lactate by dichloroacetate a pyruvate dehydrogenase kinase (PDK) inhibitor, decreases CNV development. Our data support the innovative concept of lactate as a parainflammation- and angio-metabolite associated to AMD and CNV progression. Moreover, control of blood lactate level via inhibition of PDK provides new options for the treatment of exudative AMD. This study demonstrates the ability of metabolomics for drug target discovery and opens new perspectives for AMD treatment and patient follow-up. [less ▲]

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See detailFrom Metagenomes To Industrially Relevant Enzymes
Berlemont, Renaud ULg

Conference (2010, December)

Microorganisms living in extreme conditions have now been isolated from all type of extreme environments and numerous extremozymes originating from these organisms have been described and characterized ... [more ▼]

Microorganisms living in extreme conditions have now been isolated from all type of extreme environments and numerous extremozymes originating from these organisms have been described and characterized. Indeed, most of their potential resides in their enzymes. As stated by Huston in 2008 : “As environmental concerns arise, biological tools are increasingly replacing harsh chemical and physical means of processing materials and they even harbor promise for creating cost-effective sustainable energy sources. It is imperative that we continue investigating ways in which natural products can offer economical alternatives to traditional industrial processes”. In this context the search for valuable extremophiles and their integration in industrial processes will, without any doubt, fulfill these objectives in extending the use of microorganisms and of their products to unexpected transformations. Extensive investigations on extremophiles were therefore carried out for the reason that the large diversity of these organisms allowed to think that it is probably possible to find within them the appropriate isolate that produces the best possible product for a specific application. Of course these organisms have to be unravelled and their peculiarities sometimes render the culture conditions inappropriate. The recent emergence of the metagenomic approach has, however, modified the context and has now rendered possible the investigation of the world of non cultivable microorganisms. Indeed, recent advances of the metagenomic highlighted the underestimated gene diversity contained in environmental samples. In order to isolate new valuable cold adapted proteins, Antarctica soil metagenomic libraries were constructed and screened for various activities. Screenings were performed in industrially derived conditions, when feasible, and allowed the isolation of several new lipases/esterases, proteases, amylases, xylanases, … Hence, thorough characterization of the new enzymes is required prior their integration into industrial processes. We will discuss case-studies on three enzymes primarily isolated for the agro-food industry and displaying particular features. [less ▲]

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See detailFrom meteorites to evolution and habitability of planets
Dehant, Véronique; Breuer, Doris; Claeys, Philippe et al

in Planetary and Space Science (2012)

Detailed reference viewed: 54 (7 ULg)
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See detailFrom Mixed Angles to Infinitesimals
Bair, Jacques ULg; Henry, Valérie ULg

in College Mathematics Journal (2008), 39(3), 230-233

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See detailFrom model validation to production of reference simulations: how to increase reliability and applicability of building and HVAC simulation models
Adam, Christophe; Andre, Philippe ULg; Georges, Bernard ULg et al

(2006, December)

Validation of simulation models appears from a long time as a key issue in order to promote a more intensive and more efficient use of simulation models in the field of building and HVAC simulation. IEA ... [more ▼]

Validation of simulation models appears from a long time as a key issue in order to promote a more intensive and more efficient use of simulation models in the field of building and HVAC simulation. IEA Annex 34/43 originally targeted a number of specific applications where a more advanced validation was required: ground coupling problems, multizone building, shading, day lighting and cooling load interaction, HVAC components and ventilated facades. These validation exercises were built on the large methodological experience obtained in previous projects and address sometimes very fundamental problems of heat transfer in buildings. Consulting engineers and practitioners might see these exercises as a bit too far from their objectives and it is the reason why an additional activity was proposed with the specific aim of producing, based upon the results of the validation of models, a set of reference simulations. These applications cover a range of building types (residential, commercial) and systems (production, distribution emission) and run in a variety of climates. The paper will describe how models dedicated to these applications were developed, starting from validation results, going through the selection and consolidation of simulation hypothesises and ending with a number that might be considered as reference for the concerned applications. The paper will concentrate on models required by a residential building application (multizone building equipped with a heat pump or a condensing boiler). Simulations make use of both EES and TRNSYS software and both software are applied in parallel as far as possible in the different applications in order to get a better judgment of their potential advantages and drawbacks. The use of reference simulations in view of qualifying normative methods currently in development in the frame of the European Energy Performance in Buildings Directive is also addressed and demonstrated in the paper. [less ▲]

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See detailFrom model validation to production of reference simulations: how to increase reliability and applicability of building and HVAC simulation models
Andre, Philippe ULg; Georges, Bernard ULg; Lebrun, Jean ULg et al

in Building Services Engineering Research & Technology (2008), 29(1), 61-72

Validation of simulation models appears from a long time as a key issue in order to promote a more intensive and more efficient use of simulation models in the field of building and HVAC simulation. IEA ... [more ▼]

Validation of simulation models appears from a long time as a key issue in order to promote a more intensive and more efficient use of simulation models in the field of building and HVAC simulation. IEA Annex 34/43 originally targeted a number of specific applications where a more advanced validation was required: ground coupling problems, multizone building, shading, day lighting and cooling load interaction, HVAC components and ventilated facades. These validation exercises were built on the large methodological experience obtained in previous projects and address sometimes very fundamental problems of heat transfer in buildings. Consulting engineers and practitioners might see these exercises as a bit too far from their objectives and it is the reason why an additional activity was proposed with the specific aim of producing, based upon the results of the validation of models, a set of reference simulations. These applications cover a range of building types (residential, commercial) and systems (production, distribution emission) and run in a variety of climates. The paper will describe how models dedicated to these applications were developed, starting from validation results, going through the selection and consolidation of simulation hypothesises and ending with a number that might be considered as reference for the concerned applications. The paper will concentrate on models required by a residential building application (multizone building equipped with a heat pump or a condensing boiler, heat emitted by radiators or floor heating systems). Simulations make use of both EES and TRNSYS software and both software are applied in parallel as far as possible in the different applications in order to get a better judgment of their potential advantages and drawbacks. The use of reference simulations in view of qualifying normative methods currently in development in the frame of the European Energy Performance in Buildings Directive is also addressed and demonstrated in the paper. [less ▲]

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See detailFrom models to a model?
Caponetti, Lia ULg

in Jankowitsch-Prevor, Odette; Michel, Quentin; Paile, Sylvain (Eds.) Modelling Dual-Use Trade Control Systems (2014)

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See detailFrom Modern Risks to Reflexive Society - An Alternative Approach to (Bio)terrorism
Brunet, Sébastien ULg; Claisse, Frédéric ULg; Rogister, yves

in Michel, Quentin (Ed.) Terrorisme, regards croisés - Terrorism, cross analysis (2005)

Au départ de quelques normes de portée nationale et internationale dont l'examen souligne la difficulté de parvenir à une définition opératoire du terrorisme, cet article propose un cadrage alternatif du ... [more ▼]

Au départ de quelques normes de portée nationale et internationale dont l'examen souligne la difficulté de parvenir à une définition opératoire du terrorisme, cet article propose un cadrage alternatif du phénomène terroriste fondé sur le paradigme des "risques réflexifs". Sous cet éclairage, le risque terroriste agit comme un véritable révélateur du rapport à notre environnement politique, social et technique: il est l'occasion de mettre en évidence des rapports de force, des relations politiques de domination et de dépendance qui constituent le contexte structurel de conflits locaux, régionaux ou internationaux; mais aussi, plus indirectement, en venant nous rappeler la vulnérabilité de nos collectifs, de plus en plus basés sur le fonctionnement en réseaux, l'interdépendance et le développement de macro-systèmes techniques. Il plaide pour une meilleure prise en compte de la dimension proprement politique du terrorisme et, plus largement, pour un basculement global vers un modèle de société ouverte plus sensible à des facteurs de risque trouvant leur origine dans nos propres pratiques et notre rapport au monde. Comme fil conducteur, des exemples empruntés au bioterrorisme, en ce que le phénomène paraît emblématique de la réflexivité des risques modernes. [less ▲]

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See detailFrom Monroe to Moreau: An analysis of face naming errors
Brédart, Serge ULg; Valentine, T.

in Cognition (1992), 45

Detailed reference viewed: 28 (4 ULg)
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See detailFrom mucosal resistance of larvae to behavioral fever of fingerlings: a journey in the innate immune defenses of common carp against cyprinid herpesvirus 3
Ronsmans, Maygane ULg

Doctoral thesis (2015)

Cyprinid herpesvirus 3 (CyHV-3) is a highly pathogenic virus responsible for a lethal disease in both common and koi carp (Cyprinus carpio). The common carp is one of the most important freshwater species ... [more ▼]

Cyprinid herpesvirus 3 (CyHV-3) is a highly pathogenic virus responsible for a lethal disease in both common and koi carp (Cyprinus carpio). The common carp is one of the most important freshwater species cultivated for human consumption. Its colourful subspecies koi is grown for personal pleasure and exhibitions. Both common and koi carp are economically important and since its description in the late 1990s, the CyHV-3 has caused severe financial losses in these two carp industries worldwide. Because of its economic importance and its numerous original biological properties, CyHV 3 became rapidly an attractive subject for both applied and fundamental research. The objectives of this thesis were to investigate the role of two unrelated innate immune mechanisms of carp in anti-CyHV-3 immunity. The first objective was to determine the role of epidermal mucus as an innate immune barrier against CyHV-3 entry during the early developmental stages of carp. To test this hypothesis, we investigated the sensitivity and the permissivity of carp to CyHV-3 during the early stages of its development. This hypothesis was tested using a recombinant CyHV-3 strain expressing luciferase as a constitutive reporter gene and in vivo bioluminescence imaging system. We demonstrated that carp are sensitive and permissive to CyHV 3 infection since hatching, but that their sensitivity remains relatively low in the two early developmental stages. Similarly to adults, we confirmed that the skin is the main portal of entry for the virus at early stages, and our results stress out the role of epidermal mucus as an innate immune defense of carp against pathogens even and especially at the early stages of development. The results of this study have been published in Veterinary research. The second objective of this thesis consisted to investigate whether carp express behavioral fever when infected by CyHV-3; and if so, what could be the effect of this innate immune reaction on the development of CyHV 3 disease. When infected by pathogens, both endotherms and ectotherms can express a salutary reaction by increasing their body temperature. While in endotherms this reaction is called fever and depends on intrinsic thermogenesis, ectotherms like teleosts can only upregulate their body temperature by moving to warmer places, hence the term behavioral fever. When studying the pathogenesis of CyHV-3, we observed that carp infected at 24°C (the thermal preference of healthy carp) tended to concentrate around the tank heater when it was running. This observation led us to postulate that infected subjects could express behavioral fever in natural environments where temperature gradients exist. Using multi-chamber tanks encompassing a gradient from 24°C to 32°C, we observed that carp infected by CyHV-3 express a salutary behavioral fever that completely suppresses virus induced mortalities. The relatively late onset of behavioral fever with respect to clinical signs, viral replication and cytokine upregulation led us to postulate that this phenomenon could be delayed by the virus to retain its host at a permissive temperature thereby favoring its replication and spreading. As some herpesviruses have been shown to express soluble decoy cytokine receptors, we hypothesized that CyHV-3 could express such receptor(s) able to neutralize putative pyrogenic cytokines produced by the fish. We found that CyHV-3 ORF12 encodes a soluble decoy receptor for carp Tnfα and that this viral protein makes the virus capable of delaying the migration of infected fish to warmer environments. Remarkably, the study of the molecular mechanism through which the virus alters its host’s behavior led to the discovery of the first pyrogenic cytokine in ectotherms (Tnfα). This study is the first to report the ability of a vertebrate virus to alter the behavior of its host through the expression of a single gene. This second study was submitted for publication when this thesis was printed. In conclusion, we investigated two innate immune mechanisms expressed by carp against CyHV 3 infection. The results generated in this thesis bring findings related to several scientific fields such as virology, immunology, animal behavior, evolution, ecology and even animal welfare. [less ▲]

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See detailFrom myopic coordination to resilience in socio-technical systems. A case study in a hospital
Nyssen, Anne-Sophie ULg

in Hollnagel, Erik; Paries, Jean; Woods, David (Eds.) et al Resilience Engineering in Practice: A guidebook (2011)

Detailed reference viewed: 104 (36 ULg)
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See detailFrom nature to culture? Lévi-Strauss' legacy and the study of contemporary foodways
Mescoli, Elsa ULg; Graf, Katharina

Conference (2016, July)

n the first volume of his Mythologiques, entitled The Raw and the Cooked, Claude Lévi-Strauss argued that the preparation of food is a form of language that reveals a society's structure. For Lévi-Strauss ... [more ▼]

n the first volume of his Mythologiques, entitled The Raw and the Cooked, Claude Lévi-Strauss argued that the preparation of food is a form of language that reveals a society's structure. For Lévi-Strauss, the so-called culinary triangle of the raw, the rotten and the cooked represents a semantic field within which the various forms of transformation of food from nature into culture play a key role. Since Lévi-Strauss, following extensive changes to food production, preparation and consumption, the notion of cooking has become ever more diversified, and became increasingly contentious. Yet, the multiple ways of combining and processing ingredients still give social and cultural meanings to food and trigger the creation of sociabilities and belongings through its own destruction (Gell 1986). This panel aims to explore Levi-Strauss' legacy and evaluate its usefulness in today's context from different angles, ranging from domestic food preparation to industrial production and global circulations of food. To what extent can this concept still provide an interpretative framework of topical food issues? Which contemporary myths does it shed light on? How could it be deployed to read the history of food and link it to contemporary questions? This panel welcomes papers on the history of food preparation, contemporary food preparation, including debates in professional and multimedia circles, various forms of food production and the possible transformation of food within globalised food markets. Although mobilizing a classic anthropological theory, this panel aims to be interdisciplinary and to present a diverse range of analytical perspectives. [less ▲]

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See detailFrom Near-Infrared and Raman to Surface-Enhanced Raman Spectroscopy: Progress, Limitations, Perspectives in Bioanalysis
Dumont, Elodie ULg; De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg et al

in Bioanalysis (2016), 8(10), 1077-1103

Over recent decades, spreading environmental concern entailed the expansion of green chemistry analytical tools. Vibrational spectroscopy, belonging to this class of analytical tool, is particularly ... [more ▼]

Over recent decades, spreading environmental concern entailed the expansion of green chemistry analytical tools. Vibrational spectroscopy, belonging to this class of analytical tool, is particularly interesting taking into account its numerous advantages such as fast data acquisition and no sample preparation. In this context, Near-Infrared, Raman and mainly Surface-Enhanced Raman Spectroscopy (SERS) have thus gained interest in many fields including bioanalysis. The two former techniques only ensure the analysis of concentrated compounds in simple matrices, whereas the emergence of SERS improved the performances of vibrational spectroscopy to very sensitive and selective analyses. Complex SERS substrates were also developed enabling biomarker measurements, paving the way for SERS immunoassays. Therefore, in this paper, the strengths and weaknesses of these techniques will be highlighted with a focus on recent progress. [less ▲]

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See detailFrom nets to bottom traps: is exploitation of Norway lobsters a suitable option for Corsican common spiny lobster fishermen?
Patrissi, Michela; Astrou, Adèle; Pelaprat, Corinne et al

Poster (2014, May 20)

In Corsica (NW Mediterranean), most of the fishing activity is composed of small-scale artisanal fisheries, and takes place on the western coast. The common spiny lobster (Palinurus elephas) is the main ... [more ▼]

In Corsica (NW Mediterranean), most of the fishing activity is composed of small-scale artisanal fisheries, and takes place on the western coast. The common spiny lobster (Palinurus elephas) is the main target of Corsican netters. However, its populations have been declining since the 1950's, questioning the sustainability of this activity. We therefore tried to assess whether the fishing effort, currently mostly focused on common spiny lobsters, could be moved towards other commercially-interesting deep crustaceans, such as the Norway lobster (Nephrops norvegicus), through diversification of artisanal fishing practices. With the help of local fishermen, we set up Scottish traps for Norway lobsters at depths of 300 to 400 meters, on sandy and muddy bottoms of both eastern and western coasts. Despite several tests using different baits and soak times at various depths or seasons, catches on the western coast were low. On the other hand, on the eastern coast, experimentation showed interesting yields, and large mean size (i.e. high commercial value) for both sexes. While more studies are needed to confirm these results and improve knowledge of Norway lobster stocks, trap fishing of this species on eastern coast of Corsica could be a suitable alternative for diversification of artisanal fisheries. [less ▲]

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See detailFrom Neural Stem Cells to Myelinating Oligodendrocytes
Rogister, Bernard ULg; Ben Hur, Tamir; Dubois-Dalcq, Monique

in Molecular & Cellular Neuroscience [=MCN] (1999), 14

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See detailFrom neurophysiology to genetics: cortical information processing in migraine underlies familial influences--a novel approach
Sandor, P. S.; Afra, J.; Proietti Cecchini, A. P. et al

in Functional Neurology (2000), 15(Suppl 3), 68-72

Migraine patients show impaired cortical information processing between attacks with deficient habituation of pattern-reversal visual evoked potentials (VEP), and strong intensity dependence of auditory ... [more ▼]

Migraine patients show impaired cortical information processing between attacks with deficient habituation of pattern-reversal visual evoked potentials (VEP), and strong intensity dependence of auditory cortical evoked potentials (IDAP). This could be a genetic trait as certain genetic patterns are known for evoked potentials in healthy subjects. VEP-habituation and IDAP were studied in 40 migraine patients, i.e. pairs of 20 parents and their children. We developed a novel approach based on Monte Carlo statistics to selectively assess vertical familial influences. Both groups, parents and children, were characterized by abnormal VEP-habituation and IDAP. However, similarity between related pairs was far more pronounced than similarity between unrelated pairs. Assessed with a novel statistical approach, familial influences proved to be highly significant in determining cortical information processing in migraineurs, thus supporting the important role of genetic factors. [less ▲]

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See detailFrom Newton's Fluxions to Virtual Microscopes
Bair, Jacques ULg; Henry, Valérie ULg

in Teaching Mathematics and Computer Science (2007), 5(2), 377-384

The method of fluxions was originally given by Newton in order to determine the tangent to a curve. In this note, we will formulate this method by the light of some modern mathematica tools.

Detailed reference viewed: 33 (0 ULg)