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See detailIdentification modale de systèmes variant dans le temps à partir de réponses simulées sur un exemple d'éoliennes
Bertha, Mathieu ULg; Golinval, Jean-Claude ULg

Poster (2012, November)

Wind turbines are good examples of time-varying systems as their modal properties depend on their instantaneous configuration. To catch the variations of modal parameters in time-varying systems ... [more ▼]

Wind turbines are good examples of time-varying systems as their modal properties depend on their instantaneous configuration. To catch the variations of modal parameters in time-varying systems, classical identification methods have to be adapted to the non-stationary nature of the recorded signals. In this paper, it is proposed to study the dynamic behavior of an offshore five-megawatt wind turbine. First, a numerical model of the wind turbine is created to serve as reference. Then, the time-varying behavior of the system is evaluated by simulating a large number of possible configurations. To this purpose, time responses are generated from the numerical model submitted to different environmental conditions. The wind is considered as the main non-measured external excitation force on the structure and the responses are recorded at several locations to simulate a real measurement process. Special care is brought to the accessibility of the measurement locations and to the limited number of available sensors in practice. Using these simulated measurements, output-only identification methods are used to extract varying dynamic properties of the structure. The final objective of this work is to pave the way to online condition monitoring of wind turbines. [less ▲]

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See detailIdentification of 1-methyloctyl butanoate as the major sex pheromone component from females of the saddle gall midge, Haplodiplosis marginata (Diptera: Cecidomyiidae)
Censier, Florence ULg; Fischer, Christophe ULg; Laurent, Pascal et al

in Chemoecology (2014)

The saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae), has undergone a resurgence recently as a pest of cereals in Belgium and other European countries. An effective ... [more ▼]

The saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae), has undergone a resurgence recently as a pest of cereals in Belgium and other European countries. An effective monitoring tool of saddle gall midge flights is needed in order to understand the enigmatic population dynamics of this pest, and to design an integrated management strategy. Therefore, volatile compounds emitted by females (alkan-2-ols and alk-2-yl butanoates) were identified, and the chirality of the emitted esters was determined to be the R absolute configuration. In field-trapping experiments, racemic non-2-yl butanoate attracted substantial numbers of H. marginata males. Thus, this compound will be useful in baited traps for monitoring seasonal flight patterns, and improving integrated management of the saddle gall midge in agricultural systems. [less ▲]

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See detailIdentification of 108k, 93k, and 42k Glycoproteins of Bovine Herpesvirus-1 by Monoclonal Antibodies
Baranowski, E.; Dubuisson, J.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 133(1-2), 97-111

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins gI, gIII, and gIV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by immunization of ... [more ▼]

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins gI, gIII, and gIV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by immunization of mice with either BHV-1 envelope or virus infected cells, from which the glycoproteins gI, gIII, and gIV were removed by immunoaffinity. In the latter immunization procedure mice were tolerized either against normal cellular antigens with or without glycoproteins gI, gIII, gIV, and nucleocapsid. From 154 anti-BHV-1 hybridomas isolated, 39 MAbs precipitated a 108K glycoprotein. Two other glycoproteins of respectively 42K and 93K were precipitated each by one MAb. These three glycoproteins were detected in infected cell lysate. Nine anti-108K glycoprotein MAbs neutralized BHV-1 infectivity and three non-neutralizing MAbs were able to reduce plaque development when virus was grown in the presence of these MAbs. It is therefore suggested that this glycoprotein is involved in viral entry into the cell and in cell-to-cell spread of the virus. [less ▲]

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See detailThe identification of 16O 18O 16O and 16O 16O 18O ozone isotopes in high resolution ground-based FTIR spectra
Arlander, Dixon William (Bill); Barbe, Alain; Bourgeois, M.T. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (1994), 52(3-4), 267271

We present evidence of the 16O 18O 16O and 16O 16O 18O ozone isotopes in the 5 μ region from FTIR solar occultation spectra obtained from the Jungfraujoch Solar Observatory (47°N, 8°E, 3580 m) in ... [more ▼]

We present evidence of the 16O 18O 16O and 16O 16O 18O ozone isotopes in the 5 μ region from FTIR solar occultation spectra obtained from the Jungfraujoch Solar Observatory (47°N, 8°E, 3580 m) in Switzerland at a spectral resolution of 0.0025 cm-1 (res.=1/2L). These spectra clearly show numerous unblended lines of the 16O 18O 16O ozone isotope. Laboratory spectra in the 5 μ region of both 50O3 isotopes have been measured and have yielded line positions of the v1+v3 isotopic bands which can eventually lead to their retrieval from measured ground-based solar occulation spectra. Although retrieval of 16O 16O 18O could be difficult due to slight line blending, identification of several lines is shown. [less ▲]

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See detailIdentification of a 14-kDa laminin binding protein (HLBP14) in human melanoma cells that is identical to the 14-kDa galactoside binding lectin.
Castronovo, Vincenzo ULg; Luyten, F.; van den Brule, F. et al

in Archives of Biochemistry & Biophysics (1992), 297(1), 132-8

The carbohydrate moieties present on laminin play a crucial role in the multiple biological activities of this basement membrane glycoprotein. We report the identification of a human laminin binding ... [more ▼]

The carbohydrate moieties present on laminin play a crucial role in the multiple biological activities of this basement membrane glycoprotein. We report the identification of a human laminin binding protein with an apparent molecular mass of 14 kDa on sodium dodecyl sulfate-polyacrylamide gels that was found, after purification and amino acid microsequencing, to be identical to the previously described 14-kDa galactoside binding soluble L-14 lectin. We have designated this human laminin binding protein as HLBP14. HLBP14 was purified from human melanoma cells in culture by laminin affinity chromatography and gel electroelution. We demonstrate that HLBP14 binds specifically to the poly-N-acetyllactosamine residues of murine laminin and does not bind to other glycoproteins that do not contain such structures, such as fibronectin. HLBP14 was eluted from a murine laminin column by lactose, N-acetyllactosamine, and galactose but not by other control saccharides, including glucose, fucose, mannose, and melibiose. It did not bind to laminin treated with endo-beta-galactosidase. Lactose also eluted HLBP14 off a human laminin affinity column, implying that human laminin also contains poly-N-acetyllactosamine residues. On immunoblots, polyclonal antibodies raised against HLBP14 recognized HLBP14 as well as 31- and 67-kDa molecules that are also laminin binding proteins, indicating that these proteins share common epitopes. L-14, a dimeric lactose binding lectin, is expressed in a wide variety of tissues. Although the expression of this molecule has been linked to a variety of biological events, the elucidation of its specific functions has been elusive. The observation that HLBP14, a human cancer cell laminin binding protein, is identical to L-14 strongly suggests that the functions attributed to this lectin could be mediated, at least in part, through its ability to interact with the poly-N-acetyllactosamine residues of laminin. HLBP14 could potentially play a role during tumor invasion and metastasis by modulating the interactions between cancer cells and laminin. [less ▲]

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See detailIdentification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2.
de Barsy, Marie; Jamet, Alexandre; Filopon, Didier et al

in Cellular microbiology (2011), 13(7), 1044-58

Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria are able to control the intracellular trafficking of their vacuole, presumably by the use of yet unknown ... [more ▼]

Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria are able to control the intracellular trafficking of their vacuole, presumably by the use of yet unknown translocated effectors. To identify such effectors, we used a high-throughput yeast two-hybrid screen to identify interactions between putative human phagosomal proteins and predicted Brucella spp. proteins. We identified a specific interaction between the human small GTPase Rab2 and a Brucella spp. protein named RicA. This interaction was confirmed by GST-pull-down with the GDP-bound form of Rab2. A TEM-beta-lactamase-RicA fusion was translocated from Brucella abortus to RAW264.7 macrophages during infection. This translocation was not detectable in a strain deleted for the virB operon, coding for the type IV secretion system. However, RicA secretion in a bacteriological culture was still observed in a DeltavirB mutant. In HeLa cells, a DeltaricA mutant recruits less GTP-locked myc-Rab2 on its Brucella-containing vacuoles, compared with the wild-type strain. We observed altered kinetics of intracellular trafficking and faster proliferation of the B. abortusDeltaricA mutant in HeLa cells, compared with the wild-type control. Altogether, the data reported here suggest RicA as the first reported effector with a proposed function for B. abortus. [less ▲]

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See detailIdentification of a continuous structure with a geometrical non-linearity. Part I: Conditioned reverse path method
Kerschen, Gaëtan ULg; Lenaerts, V.; Golinval, Jean-Claude ULg

in Journal of Sound & Vibration (2003), 262(4), 889-906

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex ... [more ▼]

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex systems. The aim of this paper and a companion paper is to study, by means of two methods, a continuous non-linear system consisting of an experimental cantilever beam with a geometrical non-linearity. In this paper (Part I), the ability of the conditioned reverse path method, which is a frequency domain technique, to identify the behaviour of this structure is assessed. The companion paper (Part II) is devoted to the application of proper orthogonal decomposition, which is an updating technique, to the test example. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailIdentification of a continuous structure with a geometrical non-linearity. Part II: Proper orthogonal decomposition
Lenaerts, V.; Kerschen, Gaëtan ULg; Golinval, Jean-Claude ULg

in Journal of Sound & Vibration (2003), 262(4), 907-919

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex ... [more ▼]

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex systems. The aim of this paper and a companion paper is to study, by means of two methods, a continuous non-linear system consisting of an experimental cantilever beam with a geometrical non-linearity. In the companion paper (Part I) [1] the ability of the conditioned reverse path method, which is a frequency domain technique, to identify the behaviour of this structure-is assessed. This paper (Part II) is devoted to the application of proper orthogonal decomposition, which is an updating technique, to the test example. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailIdentification of a family harboring a novel LHBéta subunit mutation associated with hypogonadism
Daly, Adrian ULg; Salvi, R.; Ménagé, J.-J. et al

in The Endocrine Society's - 88th Annual Meeting - Abstract book (2006, June)

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See detailIdentification of a fossil wood specimen in the Red Sandstone Group of Southwestern Tanzania: stratigraphical and tectonic implications.
Damblon, Freddy ULg; Gerrienne, Philippe ULg; Doutrelepont, Hughes et al

in Journal of African Earth Sciences (1998), 26

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See detailIdentification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal.
Braem, Caroline V; Kas, Koen; Meyen, Eva et al

in Journal of Biological Chemistry (2002), 277(22), 19673-8

The activation of the pleomorphic adenoma gene 1 (PLAG1) is the most frequent gain-of-function mutation found in pleomorphic adenomas of the salivary glands. To gain more insight into the regulation of ... [more ▼]

The activation of the pleomorphic adenoma gene 1 (PLAG1) is the most frequent gain-of-function mutation found in pleomorphic adenomas of the salivary glands. To gain more insight into the regulation of PLAG1 function, we searched for PLAG1-interacting proteins. Using the yeast two-hybrid system, we identified karyopherin alpha2 as a PLAG1-interacting protein. Physical interaction between PLAG1 and karyopherin alpha2 was confirmed by an in vitro glutathione S-transferase pull-down assay. Karyopherin alpha2 escorts proteins into the nucleus via interaction with a nuclear localization sequence (NLS) composed of short stretches of basic amino acids. Two putative NLSs were identified in PLAG1. The predicted NLS1 (KRKR) was essential for physical interaction with karyopherin alpha2 in glutathione S-transferase pull-down assay, and its mutation resulted in decreased nuclear import of PLAG1. Moreover, NLS1 was able to drive the nuclear import of the cytoplasmic protein beta-galactosidase. In contrast, predicted NLS2 of PLAG1 (KPRK) was not involved in karyopherin alpha2 binding nor in its nuclear import. The residual nuclear import of PLAG1 after mutation of the NLS1 was assigned to the zinc finger domain of PLAG1. These observations indicate that the nuclear import of PLAG1 is governed by its zinc finger domain and by NLS1, a karyopherin alpha2 recognition site. [less ▲]

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See detailIdentification of a magnetic anomaly at Jupiter from satellite footprints
Grodent, Denis ULg

E-print/Working paper (2004)

Repeated imaging of Jupiter's aurora has shown that the northern main oval has a distorted 'kidney bean' shape in the general range of 90-140? System III longitude, which appears unchanged since 1994 ... [more ▼]

Repeated imaging of Jupiter's aurora has shown that the northern main oval has a distorted 'kidney bean' shape in the general range of 90-140? System III longitude, which appears unchanged since 1994. While it is more difficult to observe the conjugate regions in the southern aurora, no corresponding distortion appears in the south. Recent improved accuracy in locating the satellite footprint auroral emissions has provided new information about the geometry of Jupiter's magnetic field in this and other areas. The study of the magnetic field provides us with insight into the state of matter and the dynamics deep down Jupiter. There is currently no other way to do this from orbit. The persistent pattern of the main oval implies a disturbance of the local magnetic field, and the increased latitudinal separation of the locus of satellite footprints from each other and from the main oval implies a locally weaker field strength. It is possible that these phenomena result from a magnetic anomaly in Jupiter's intrinsic magnetic field, as was proposed by A. Dessler in the 1970's. There is presently only limited evidence from the scarcity of auroral footprints observed in this longitude range. We propose to obtain HST UV images with specific observing geometries of Jupiter to determine the locations of the auroral footprints of Io, Europa, and Ganymede in cycle 13 to accurately determine the magnetic field geometry in the suggested anomaly region, and to either confirm or refute the suggestion of a local magnetic anomaly. [less ▲]

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See detailIdentification of a microRNA landscape targeting the PI3K/Akt signaling pathway in inflammation-induced colorectal carcinogenesis
JOSSE, Claire ULg; Bouznad, Nassim ULg; Geurts, Pierre ULg et al

in American Journal of Physiology - Gastrointestinal and Liver Physiology (2014), 306

Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS ... [more ▼]

Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced mouse model of colitis-associated cancer was used to analyze microRNA (miRNA) modulation accompanying inflammation-induced tumor development and to determine whether inflammation-triggered miRNA alterations affect the expression of genes or pathways involved in cancer. A miRNA microarray experiment was performed to establish miRNA expression profiles in mouse colon at early and late time points during inflammation and/or tumor growth. Chronic inflammation and carcinogenesis were associated with distinct changes in miRNA expression. Nevertheless, prediction algorithms of miRNA-mRNA interactions and computational analyses based on ranked miRNA lists consistently identified putative target genes that play essential roles in tumor growth or that belong to key carcinogenesis-related signaling pathways. We identified PI3K/Akt and the insulin growth factor-1 (IGF-1) as major pathways being affected in the AOM/DSS model. DSS-induced chronic inflammation downregulates miR-133a and miR-143/145, which is reportedly associated with human colorectal cancer and PI3K/Akt activation. Accordingly, conditioned medium from inflammatory cells decreases the expression of these miRNA in colorectal adenocarcinoma Caco-2 cells. Overexpression of miR-223, one of the main miRNA showing strong upregulation during AOM/DSS tumor growth, inhibited Akt phosphorylation and IGF-1R expression in these cells. Cell sorting from mouse colons delineated distinct miRNA expression patterns in epithelial and myeloid cells during the periods preceding and spanning tumor growth. Hence, cell-type-specific miRNA dysregulation and subsequent PI3K/Akt activation may be involved in the transition from intestinal inflammation to cancer. [less ▲]

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See detailIdentification of a new aphid isoprenyl diphosphate synthase
Vandermoten, Sophie ULg; Beliveau, Catherine; Sen, Stéphanie et al

Conference (2006, November)

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See detailIdentification of a Nonlinear Wing Structure Using an Extended Modal Model
Platten, M. F.; Wright, J. R.; Cooper, J. E. et al

in Journal of Aircraft (2009), 46(5), 1614-1626

The nonlinear resonant decay method identifies a nonlinear dynamic system using a model based in linear modal space comprising the underlying linear system and a small number of additional terms that ... [more ▼]

The nonlinear resonant decay method identifies a nonlinear dynamic system using a model based in linear modal space comprising the underlying linear system and a small number of additional terms that represent the nonlinear behavior. In this work, the method is applied to an aircraftlike wing/store/pylon experimental structure that consists of a rectangular wing with two stores suspended beneath it by means of nonlinear pylons with a nominally hardening characteristic in the store rotation degree of freedom. The nonlinear resonant decay method is applied to the system using multishaker excitation. The resulting identified mathematical model features five modes, two of which are strongly nonlinear, one is mildly nonlinear, and two are completely linear. The restoring force surfaces obtained from the mathematical model are in close agreement with those measured from the system. This experimental application of the nonlinear resonant decay method indicates that the method could be suitable for the identification of nonlinear models of aircraft in ground vibration testing. [less ▲]

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See detailIdentification of a novel autoantigen in inflammatory bowel disease by protein microarray.
Vermeulen, Nathalie; de Beeck, Katrijn Op; Vermeire, Severine et al

in Inflammatory Bowel Diseases (2011), 17(6), 1291-300

BACKGROUND: Patients with inflammatory bowel disease (IBD) display immunoreactivity to self-antigens and microbial antigens. We used a protein microarray approach to identify novel autoantigens in IBD ... [more ▼]

BACKGROUND: Patients with inflammatory bowel disease (IBD) display immunoreactivity to self-antigens and microbial antigens. We used a protein microarray approach to identify novel autoantigens in IBD. METHODS: ProtoArray Human Protein Microarray v4.0 containing 8268 human proteins from Invitrogen (La Jolla, CA) was used. RESULTS: Twenty-five IBD patients and five healthy controls were screened for candidate autoantigens. For 256 antigens, IBD patients had a higher seroreactivity than controls. Twenty antigens were selected for further evaluation in a larger cohort (60 ulcerative colitis [UC] patients, 60 Crohn's disease [CD] patients, 60 healthy controls, and 60 gastrointestinal-diseased controls) by means of a customized protein microarray. Out of these 20 antigens, one antigen, family with sequence similarity 84 member A (FAM84A), was identified as a target antigen in IBD. Antibodies to FAM84A were significantly more prevalent in IBD patients (19%) than in gastrointestinal-diseased controls (1.7%) (P = 0.0008) and healthy controls (5%) (P = 0.01). Anti-FAM84A antibodies were found in 26.6% of UC patients and in 11.7% of CD patients. FAM84A was confirmed as target antigen in IBD by means of Western blotting in a large independent cohort (100 UC patients, 106 CD patients, 102 healthy controls, and 100 gastrointestinal-diseased controls). Antibodies to FAM84A were significantly more prevalent in IBD patients (20%) than in gastrointestinal-diseased controls (5%) (P = 0.0004) and healthy controls (0%) (P < 0.0001). Anti-FAM84A antibodies were found in 18% of UC patients and in 22% of CD patients. CONCLUSIONS: We identified FAM84A as a novel autoantigen in IBD. (Inflamm Bowel Dis 2011;). [less ▲]

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See detailIdentification of a novel snake peptide displaying high affinity and antagonist behaviour for the alpha2-adrenoreceptors
Rouget, Céline; Quinton, Loïc ULg; Maïga, Arhamatoulaye et al

in British Journal of Pharmacology (2010), 161

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