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See detailIn vitro hemocompatibility of nanocarriers tailored for biopharmaceutical drugs.
Sevrin, Chantal ULg; Cerda Cristerna, Bernardino Isaac ULg; Lombart, François ULg et al

Poster (2012, May 06)

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed ... [more ▼]

The optimization of nanoparticles (NP) for drug delivery, in particular to target the BBB, imposes to verify their hemocompatibility both for toxicological and efficiency of targeting perspectives. Indeed the large surface they are able to expose to the biological environment promotes their interaction with various biochemicals, in particular proteins which can after adsorption elicit the activation of biological cascades either responsible from NP clearance or/and harmful body reaction (inflammatory / coagulation). In the frame of the European Integrated Project : “Nanobiopharmaceutics”, we have the opportunity to compare the hemoreactivity of about 145 different NP samples differing in core and surface chemistry and classified according to their expected difference in hydrophobicity based on the nature of their core materials. According to this classification, PLGA nanoparticles, polyglycidol-polyethyethylene oxide nanoparticles, polyglycidol thyolated or polyacrylamide nanogels, and polyelectrolyte complexes either based on polyamidoamine or poly(N,N-dimethylamino- 2-ethylmethacrylate) have been evaluated within a concentration ranging from 0.3 to 1000 μg/mL. These in vitro tests have been realized for screening purpose adopting normal human bloods and according to Iso 10993. As a summary of this extensive study, our results clearly highlight that most of the polymeric nanoparticles evaluated give rise to some alterations of the blood components. In particular the platelets, intrinsic pathway of coagulation and complement activation are the most reactive biological parameters in the presence of these nanostuctures. Although not strictly related to the surface chemistry our classification has also allowed us to derive some clear correlations between nanomaterial properties and their hemoreactivity. Within the class of polyelectrolyte electrolyte complexes, the modifications brought in the surface chemistry has drastically improved their hemoreactivity [less ▲]

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See detailIn vitro identification of targeting ligands of human M cells by phage display
Fievez, V.; Plapied, L.; Plaideau, C. et al

in International Journal of Pharmaceutics (2010), 394(1-2), 35-42

To improve transport of vaccine-loaded nanoparticles, the phage display technology was used to identify novel lead peptides targeting human M cells. Using an in vitro model of the human follicle ... [more ▼]

To improve transport of vaccine-loaded nanoparticles, the phage display technology was used to identify novel lead peptides targeting human M cells. Using an in vitro model of the human follicle-associated epithelium (FAE) which contains both Caco-2 and M cells, a T7 phage display library was screened for its ability either to bind the apical cell surface of or to undergo transcytosis across Caco-2 cells or FAE. The selection for transcytosis across both enterocytes and FAE identified three different peptide sequences (CTGKSC, PAVLG and LRVG) with high frequency. CTGKSC and LRVG sequences enhanced phage transport across M-like cells. When polymeric nanoparticles were grafted with the sequences CTGKSC and LRVG, their transport by FAE was significantly enhanced. These peptides could therefore be used to enhance the transport of vaccine-loaded nanoparticles across the intestinal mucosal barrier. [less ▲]

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See detailIn vitro induced imbalance between stromelysin and TIMP-1 production by human chondrocytes
Henrotin, Yves ULg; Labasse, A; Crielaard, Jean-Michel ULg et al

in Osteoarthritis and Cartilage (1999), 7(SA), 33

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See detailThe in vitro influences of neurotensin on the motility characteristics of human U373 glioblastoma cells
Servotte, S.; Camby, I.; Debeir, O. et al

in Neuropathology & Applied Neurobiology (2006), 32(6), 575-584

Astrocytic tumours are associated with dismal prognoses due to their pronounced ability to diffusely invade the brain parenchyma. Various neuropeptides, including gastrin, are able to modulate tumour ... [more ▼]

Astrocytic tumours are associated with dismal prognoses due to their pronounced ability to diffusely invade the brain parenchyma. Various neuropeptides, including gastrin, are able to modulate tumour astrocyte migration. While neurotensin has been shown to influence the proliferation of glioma cells and the migratory ability of a large set of other cell types, its role in glioma cell migration has never been investigated. Neurotensin-induced modifications to the motility features of human U373 glioblastoma cells therefore constitute the topic of the present study. We evidenced that three subtypes of neurotensin receptors (NTR1, NTR2 and NTR3) are expressed in U373 glioblastoma cells, at least as far as their mRNAs are concerned. Treating U373 tumour cells with 10 nM neurotensin markedly modified the morphological patterns of these cells and also profoundly altered the organization of their actin cytoskeletons. Pull-down assays revealed that neurotensin induced the activation in U373 cells of both Rac1 and Cdc42 but not RhoA. Scratch wound assays evidenced that neurotensin (0.1 and 10 nM) very significantly inhibited wound colonization by U373 cells cultured in the absence of serum. In addition, quantitative phase-contrast videomicroscopy analyses showed that neurotensin decreases the motility levels of U373 glioblastoma cells when these cells are cultured on plastic. In sharp contrast, neurotensin stimulates the motility of U373 cells when they are cultured on laminin, which is a pro-adhesive extracellular matrix component ubiquitously secreted by glioma cells. Our data thus strongly suggest that, in addition to gastrin, neurotensin is a neuropeptide capable of modulating tumour astrocyte migration into the brain parenchyma. [less ▲]

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See detailIn Vitro Inhibitory Effect of SR 27417, a Potent Platelet-Activating Factor (PAF) Receptor Antagonist, on the PAF-Induced Bovine Platelet Aggregation
Bastos da Silva, Miriam; Delaunois, Annie ULg; Gustin, Pascal ULg et al

in Veterinary Research (2000), 31(2, Mar-Apr), 267-672

The in vitro inhibitory effect of SR 27417, an antagonist of the platelet-activating factor (PAF) receptor, on PAF-induced platelet aggregation was studied in blood collected from seven healthy Friesien ... [more ▼]

The in vitro inhibitory effect of SR 27417, an antagonist of the platelet-activating factor (PAF) receptor, on PAF-induced platelet aggregation was studied in blood collected from seven healthy Friesien calves. Inhibitory effects of SR 27417 were determined at thirteen different concentrations (0.1-400 nM) by using the dose-response curves of PAF on calf platelet aggregation. In the presence of SR 27417, the maximal slopes of aggregation (%/min) induced by low and high concentrations of PAF were significantly different from the control values obtained without an antagonist at p < or = 0.05 and p < or = 0.01 respectively. In vitro PAF-induced calf platelet aggregation was dose-dependently inhibited by SR 27417. The drug inhibited PAF-induced platelet aggregation in a competitive reversible manner (pA2 = 10.46 +/- 2.36 mol x L(-1)). In conclusion, the results of our study showed that addition of SR 27417 to bovine platelet in vitro inhibits PAF-induced platelet aggregation. [less ▲]

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See detailIn vitro investigations of smart drug delivery systems based on redox-sensitive cross-linked micelles
Cajot, Sébastien; Schol, D.; Dahnier, F. et al

in Macromolecular Symposia (2013), 13(12), 1661-1670

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly ... [more ▼]

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly(α-azide-ϵ-caprolactone). Stability of these micelles is insured in diluted media by cross-linking their core via the addition of a bifunctional cross-linker, while redox sensitivity is provided to these micelles by inserting a disulfide bridge in the cross-linker. The potential of these responsive micelles to be used as nanocarriers is studied in terms of cytotoxicity and cellular internalization. The release profiles are also investigated by varying the environment reductive strength. [less ▲]

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See detailIn Vitro Kinetics of a Newborn Rat Astroglia-Derived Neuronotoxic Activity
Leprince, Pierre ULg; rigo, Jean-Michel; Lefebvre, Philippe ULg et al

in Neuroscience Letters (1989), 102(2-3), 268-72

A low-molecular weight astrocyte-derived neuronotoxic activity (ANTA) was detected, using a colorimetric bioassay of cell survival, by its effect on cultured granule cells. This neuronotoxic activity was ... [more ▼]

A low-molecular weight astrocyte-derived neuronotoxic activity (ANTA) was detected, using a colorimetric bioassay of cell survival, by its effect on cultured granule cells. This neuronotoxic activity was found to be released rapidly from newborn rat astrocytes in culture upon incubation in 50 mM K+-containing growth medium. The release by astrocytes could be induced repetitively by successive incubations in high-K+ medium alternating with incubations in normal medium. Astrocytes were also found to inactivate rapidly isobutanol-extracted ANTA in normal K+-containing growth medium. Kinetic studies showed that ANTA induces a slow (greater than 12 h) degeneration of cultured granule cells. ANTA is shown here to be an intermediate of normal astrocyte metabolism and to display appropriate kinetic characteristics compatible with its proposed role in inducing part of the delayed neuronal loss that occurs after a brain injury (secondary neuronal death). [less ▲]

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See detailIn vitro maturation treatment affects developmental competence of laparoscopic ovum pickup-derived oocytes in follicle-stimulating hormone-stimulated goats
Locatelli, Y; Poulin, N; Baril, G et al

in Reproduction, Fertility, & Development (2008), 20(1), 182-183

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU ... [more ▼]

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU sessions were performed on 33 adult goats of the Saanen and Alpine breeds. Females were synchronized (Day 0) during the nonbreeding season by inserting vaginal sponges (45 mg of fluorogestone acetate, Intervet, Boxmeer, The Netherlands). At Day 8, an i.m. injection of 50 μg of cloprostenol (Estrumate; Schering-Plough Animal Health, Pointe-Claire, Quebec, Canada) was administered. Porcine FSH (Stimufol, Merial, Brussels, Belgium, 160 mg/goat) was administered in 5 injections at 12-h intervals, starting on Day 8. The LOPU took place under general anesthesia on Day 11, and follicles ≥2 mm were aspirated with an 18-gauge needle connected to a controlled vacuum system. Vaginal sponges were removed at the time of LOPU. Treatments were repeated 2 times in a 2-week interval scheme (2 goats and 1 goat were excluded from the experiment during the second and third LOPU sessions, respectively). Cumulus–oocyte complexes were washed and evaluated for quality (graded from 1 to 3). Oocytes recovered from unstimulated slaughterhouse-derived ovaries served as a control. Cumulus–oocytes complexes from Grades 1 and 2 were submitted to IVM in TCM-199, supplemented with 100 μm of cysteamine and either 10 ng mL–1 of epidermal growth factor (EGF) or 10% follicular fluid and 100 ng mL–1 of ovine FSH (FF-FSH). Matured oocytes were then submitted to IVF and in vitro development as described by Cognié et al. (2004 Reprod. Fertil. Dev. 16, 437–445). Over the 94 LOPU sessions, 20.4 ± 0.9 follicles were aspirated (mean ± SEM), allowing the recovery of 12.3 ± 0.7 COC per goat and per session, of which 80.1% were suitable for IVM (Grades 1 and 2). Results of in vitro production are detailed in the table. The IVM treatment did not significantly affect cleavage or blastocyst development rates in oocytes derived from slaughterhouse ovaries. Cleavage rates were significantly decreased in LOPU-derived oocytes when compared with control oocytes. For LOPU-derived oocytes, cleavage and final blastocyst development rates were increased significantly and kinetics of embryo development were accelerated when FF-FSH was used during IVM as compared with EGF. The IVM with FF-FSH allowed us to produce 4.1 blatocysts per goat per LOPU session. These results demonstrate the interest in LOPU for goat embryo production once appropriate IVM treatment is used. The difference observed between LOPU and slaughterhouse oocytes in terms of response to IVM treatments may be related to FSH stimulation prior to the LOPU session or to postmortem changes in oocyte responsiveness in the slaughterhouse group. [less ▲]

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See detailIn vitro micropropagation of Jatropha curcas L. from bud aggregates
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Druart, Phillipe et al

in Journal of Technology Innovations in Renewable Energy (2013), 2

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 ... [more ▼]

Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l-1 citric acid, 12.2 mg.l-1 adenine sulfate, 15 mg.l-1 L-arginine, 2.46 µM IBA (indole-3-butyric acid), 30 g.l-1 sucrose and 7 g.l-1 of agar, and enriched with different balances of BA (benzyladenine) and L glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 µM BA and 25 mg.l 1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in a MS medium supplemented with 2.21 µM BA, 5.70 µM IAA (indole-3-acetic acid) and 15 mg.l-1 L arginine. These shoots were isolated and then rooted in MS containing 2.46 µM of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable. [less ▲]

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See detailIn vitro modelisation of prions neuroinvasion mediated by dendritic cells
Dorban, G.; Defaweux, Valérie ULg; Heinen, Ernst ULg et al

Poster (2008, October)

Detailed reference viewed: 15 (10 ULg)
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See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Labasse, A; Zheng, SX et al

in Rheumatology in Europe (1998), 27(S2), 7

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See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULg; Reginster, Jean-Yves ULg

in Reginster, Jean-Yves; Pelletier, J-P; Martel-Pelletier, J (Eds.) et al Osteoarthritis: Clinical and experimental aspects (1999)

Detailed reference viewed: 9 (2 ULg)
See detailIn vitro models of non persistent and persistent infection of human and murine neuroblastoma cell lines by the varicella zoster virus
Schlabertz, Tania; Sadzot-Delvaux, Catherine ULg; Piette, Jacques ULg et al

in Archives Internationales de Physiologie et de Biochimie (1997)

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See detailIn vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL.
Van Heusden, Alain ULg; Nusgens, Betty ULg; Goffinet, Gerhard ULg et al

in Journal of Periodontal Research (1998), 33(6), 377-85

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce ... [more ▼]

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium. [less ▲]

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See detailIn vitro modulation of human gingival keratinocyte migration by minocycline-HCl
Van Heusden, Alain ULg; Nusgens, Betty ULg; Lapière, Charles et al

in Journal of Dental Research (1998), 77

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See detailIn vitro modulation of keratinocyte attachment by minocycline H-Cl
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

in Journal of Dental Research (1995), 74

Detailed reference viewed: 14 (3 ULg)
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See detailIn vitro modulation of keratinocyte attachment by minocycline HCl.
Van Heusden, Alain ULg; Rompen, Eric ULg; Lebfevre, P. et al

Poster (1994)

Detailed reference viewed: 6 (0 ULg)