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See detailHoxc5 and Hoxc8 Expression Are Selectively Turned on in Human Cervical Cancer Cells Compared to Normal Keratinocytes
Alami, Y.; Castronovo, Vincenzo ULg; Belotti, D. et al

in Biochemical and Biophysical Research Communications (1999), 257(3), 738-45

A growing number of data have sustained the involvement of homeobox genes expression deregulation in cancer. In this study, we have performed an exhaustive survey of the expression of the 39 class I HOX ... [more ▼]

A growing number of data have sustained the involvement of homeobox genes expression deregulation in cancer. In this study, we have performed an exhaustive survey of the expression of the 39 class I HOX genes expressed in normal and malignant human cervix keratinocytes. Using RT-PCR, we observed that the vast majority (34/39) of HOX genes are expressed in normal keratinocytes. Only HOXA2, HOXA7, HOXC5, HOXC8 and HOXD12 were found to be silent. Interestingly, this pattern is conserved in the transformed keratinocytes (SiHa cells) except for the appearance of HOXC5 and HOXC8 mRNA. The HOXC5 and HOXC8 expression was also observed in two other transformed keratinocytes cell lines of independent origins, Eil-8 and 18-11S3, and confirmed by in situ hybridization. Our data add weight to the body of evidence attributing to a specific adult tissue a particular combination of expressed HOX genes and suggest that HOXC5 and/or HOXC8 could be involved in the process leading to the transformation of cervical keratinocytes. [less ▲]

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See detailThe HOXC6 homeodomain-containing proteins
Chariot, Alain ULg; Gielen, jacques

in International Journal of Biochemistry & Cell Biology (1998), 30

The HOXC6 homeodomain-containing proteins act as transcription factors in the genetic control of multiple genes involved in development and cell differentiation. Two HOXC6 polypeptides are encoded by a ... [more ▼]

The HOXC6 homeodomain-containing proteins act as transcription factors in the genetic control of multiple genes involved in development and cell differentiation. Two HOXC6 polypeptides are encoded by a single homeobox ('HOX') gene described as 'master gene' for the crucial role it plays in the patterning and axial morphogenesis of multiple species. Transcription of the HOXC6 gene is initiated from two promoters and generates two proteins that share the same DNA-binding domain but harbor a distinct N-terminal region. Recent studies have demonstrated that both HOXC6 products can activate or repress transcription, depending on the cellular context. Functional in vivo specificity of HOXC6 proteins may be achieved through combinatorial interactions with other members of the HOX family as well as with co-factors whose identities are largely unknown. Disruption of this 'HOX code' may lead to pathology such as developmental defects. [less ▲]

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See detailHpatitis C infection: eligibility for antiviral therapies
El souda, R; DELWAIDE, Jean ULg; GERARD, Christiane ULg et al

in Acta Gastro-Enterologica Belgica (2004), 67

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See detailHPLC determination of fenofibric acid in human plasma using automated solid phase extraction as sample preparation
Streel, B.; Zimmer, C.; Sibenaler-Dechamps, R. et al

in Journal de Pharmacie de Belgique (1995), 50

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See detailHPLC enantiomeric separation of B-blocking drugs using an alpha 1-acid glycoprotein column
Ceccato, Attilio ULg; Hubert, Philippe ULg; Streel, Bruno et al

in Journal de Pharmacie de Belgique (1993), 48

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See detailHPLC in neurochemistry
Bontemps, J; Laschet, L; Bettendorff, Lucien ULg et al

Poster (1984, May 18)

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See detailHPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate
Innocente, Nadia; Biasutti, Marialuisa; Blecker, Christophe ULg

in International Dairy Journal (2011), 21(4), 222-228

Extraction of proteose-peptones (PPs) was carried out from fresh milk, from milk after prolonged incubation (12 days at 37 degrees C) and from two different whey protein concentrates (WPCs). The high ... [more ▼]

Extraction of proteose-peptones (PPs) was carried out from fresh milk, from milk after prolonged incubation (12 days at 37 degrees C) and from two different whey protein concentrates (WPCs). The high performance liquid chromatography profiles of these extracts were compared. PPs eluted as three chromatographic peaks, which increased during milk incubation. The PP extracts from WPCs showed a number of peaks that are attributable to small peptides belonging to the proteose-peptone fraction and probably derive from proteolysis during storage of WPC. The dynamic properties at the air-water interface of the PP extracts were investigated by the drop-volume method. The PPs extracted from fresh milk showed the lowest values and a more rapid reduction in surface tension. PPs from WPCs were found to be effective as surfactants, even though a less marked reduction in surface tension compared with PPs from milk samples was shown. (C) 2011 Elsevier Ltd. All rights reserved. [less ▲]

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See detailHPLC quantification of alkaloids from Haplophyllum extracts and comparison with their cytotoxic properties
Fiot, Julien; Jansen, Olivia ULg; Akhmedjanova, Valentina et al

in Phytochemical Analysis (2006), 17(5), 365-369

An efficient system for the analysis of total alkaloids extracted from the aerial parts from different species of genus Hoplophyllum (Rutaceac) by HPLC on a reversed-phase column is described. The HIPLC ... [more ▼]

An efficient system for the analysis of total alkaloids extracted from the aerial parts from different species of genus Hoplophyllum (Rutaceac) by HPLC on a reversed-phase column is described. The HIPLC method described was validated for its specificity, linearity and precision using external standards (haplopine, skimmianine and haplamine). The chromatographic conditions allowed the separation of alkaloids and the quantification of haplopine, skimmianine and haplamine in different samples of species of Haplophyllum collected in Uzbekistan. The alkaloidal contents of samples were compared with their in vitro cytotoxic properties against two cancer cell lines (HeLa. and HCT-116). The cytotoxicity of extracts was correlated with the concentration of haplopine, skimmianine or haplamine in aerial parts of species of Haplophyllum. Copyright (c) 2006 John Wiley [less ▲]

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See detailA HPTLC densitometric determination of flavonoids from Passiflora alata, P-edulis, P-incarnata and P-caerulea and comparison with HPLC method
Pereira, C. A. M.; Yariwake, J. H.; Lancas, F. M. et al

in Phytochemical Analysis (2004), 15(4, JUL-AUG), 241-248

A high-performance thin layer chromatographic (HPTLC) method was developed in order to determine quantitatively the flavonoids in leaves of Passiflora alata, P. edulis, P. caerulea and P. incarnata. The ... [more ▼]

A high-performance thin layer chromatographic (HPTLC) method was developed in order to determine quantitatively the flavonoids in leaves of Passiflora alata, P. edulis, P. caerulea and P. incarnata. The content of orientin and isoorientin was determined, and the results were compared with those obtained using a quantitative HPLC-UV method. The latter employed rutin as standard and was developed to analyse flavonoid content from Passiflora leaves for the purpose of ensuring the quality of Passiflora phytomedicines. The results obtained using the two methods indicate that there are qualitative and quantitative differences in the flavonoids of the reference Passiflora species studied. The two methods were also employed to analyse commercial samples to illustrate their application in qualitative ('fingerprint') and quantitative determination, demonstrating their feasibility in the quality control of flavonoids from crude Passiflora drugs and phytomedicines. The HPLC conditions used are also suitable for the quantitative analysis of aqueous extracts (Passiflora infusions). Copyright (C) 2004 John Wiley Sons, Ltd. [less ▲]

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See detailHPV DNA testing in population-based cervical screening (VUSA-Screen study): results and implications.
Rijkaart, D. C.; Berkhof, J.; van Kemenade, F. J. et al

in British journal of cancer (2012), 106(5), 975-81

BACKGROUND: Human papillomavirus (HPV) testing is more sensitive than cytology for detecting high-grade cervical intraepithelial neoplasia (CIN). We evaluated the performance of high-risk HPV (hrHPV ... [more ▼]

BACKGROUND: Human papillomavirus (HPV) testing is more sensitive than cytology for detecting high-grade cervical intraepithelial neoplasia (CIN). We evaluated the performance of high-risk HPV (hrHPV) testing in routine screening. METHODS: In all, 25,871 women (29-61) enrolled in our population-based cohort study were offered both cytology and hrHPV testing. High-risk HPV-positive women with normal cytology and an age-matched subcohort of hrHPV-negative women with normal cytology were invited for repeat testing after 1 and/or 2 years and were referred for colposcopy if they presented with abnormal cytology and/or a positive hrHPV test. The hrHPV-positive women with borderline or mild dyskaryosis (BMD) and all women with moderate dyskaryosis or worse (>BMD) were directly referred for colposcopy. Women with BMD and an hrHPV-negative test were advised to repeat cytology at 6 and 18 months and were referred for colposcopy if the repeat cytology test was abnormal. The main outcome measure was CIN grade 3 or worse (CIN3+). Results were adjusted for non-attendance at repeat testing. RESULTS: The hrHPV-positive women with abnormal cytology had a CIN3+ risk of 42.2% (95% confidence interval (CI): 36.4-48.2), whereas the hrHPV-positive women with normal cytology had a much lower risk of 5.22% (95% CI: 3.72-7.91). In hrHPV-positive women with normal cytology, an additional cytology step after 1 year reduced the CIN3+ risk to only 1.6% (95% CI: 0.6-4.9) if the repeat test was normal. The CIN3+ risk in women with hrHPV-positive normal cytology was higher among women invited for the first time (29-33 years of age) (9.1%; 95% CI: 5.6-14.3) than among older women (3.0%; 95% CI: 1.5-5.5). CONCLUSION: Primary hrHPV screening with cytology triage in women aged >/=30 years is an effective way to stratify women on CIN3+ risk and seems a feasible alternative to cytological screening. Repeat cytology after 1 year for hrHPV-positive women with normal cytology is however necessary before returning women to routine screening. [less ▲]

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See detailHPV triggers NK cell cytotoxic activity and cytokine secretion
Jacobs, Nathalie ULg; Renoux, Virginie ULg; Bisig, Bettina ULg et al

Conference (2011)

Background The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients ... [more ▼]

Background The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients. Around 90% of HPV-infected women will clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has been performed evaluating the direct interaction between HPV and Natural Killer (NK) cells although these cells play a key role in host resistance to virus and tumor. Methods/Results By immunochemistry, we demonstrated an NK cell infiltration in HPV+ squamous pre-neoplasic lesions. Since HPV cannot grow in vitro, virus-like particles (VLP) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed a higher cytotoxic activity (CD107 and chromium release assays) and cytokine production (TNF-α and IFN-γ) in the presence of HPV-VLP. Uptake of HPV-VLP by dendritic cells (DC) has been shown to induce their activation, therefore, we investigated by flow cytometry and microscopy whether the stimulation of NK cell activity is linked to VLP internalization. We observed a faster entry into these cells compared to DC. Furthermore, virus uptake by NK cells is mediated by macropinocytosis, whereas this entry is dependent on clathrin or caveolin endocytosis pathways in DC. Using NK cell lines expressing or not CD16 and blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, but also for degranulation and cytokine production. Conclusion Thus, we show for the first time that NK cells interact with HPV and could participate in the immune response against HPV-induced tumors. [less ▲]

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See detailHR 7674: a low-amplitude cepheid?
Manfroid, Jean ULg; Renson, Pierre ULg; Burnet, M.

in Information Bulletin on Variable Stars (1997), 4453

Not Available

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See detailHR Carinae - A luminous blue variable surrounded by an arc-shaped nebula
Hutsemekers, Damien ULg; van Drom, E.

in Astronomy and Astrophysics (1991), 248

New high and medium dispersion spectroscopic observations of the luminous blue variable (LBV) star HR Car are presented, showing that this star has a multiple shell expanding atmosphere and undergoes ... [more ▼]

New high and medium dispersion spectroscopic observations of the luminous blue variable (LBV) star HR Car are presented, showing that this star has a multiple shell expanding atmosphere and undergoes spectral variations apparently correlated with the light variations similarly to the other LBVs. Most striking are the very broad emission wings (FWZI of about 3000 km/s) of the Balmer lines, reminiscent from the broad lines observed in the spectra of Of and WR stars. A kinematic distance of 5.4 kpc and a bolometric magnitude of -9.4 put HR Car among the most luminous stars of the Galaxy. The narrow band filter imagery also reveals an arc-shaped jet like nebula associated with HR Car. Spectroscopic observations of this nebula reveal an emission nature with very low excitation. Like the star itself, the nebula is characterized by a significant N/O overabundance, probably due to the presence of nuclear processed material ejected by the star. [less ▲]

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See detailHR managers views and practices in SME's regarding return to work of sick listed employees
Mairiaux, Philippe ULg; Lequeux, Sarah; Lambreghts, C et al

Poster (2012, October 24)

Background Little is known about the return to work (RTW) policies implemented in small and medium size enterprises (SME) which have limited resources to define and apply such policies. This study aimed ... [more ▼]

Background Little is known about the return to work (RTW) policies implemented in small and medium size enterprises (SME) which have limited resources to define and apply such policies. This study aimed at assessing the existing return to work practices in SME’s and exploring their view regarding the help they could receive from their occupational health service. Methods HR managers of 46 SME’s (23 in Flanders, 23 in Wallonia) employing 100 to 200 employees were contacted for an interview. The manager was asked to fill in a written questionnaire and to comment his/her answers in an open discussion with the researcher. The questionnaire involved three parts: 1°) describing existing rules or procedures when returning to work after sick leave, 2) assessing the manager knowledge of the RTW legal regulations within the occupational health care system, 3) assessing the occupational health physician (OP) involvement in RTW and the enterprise expectations for the future. Results Participation to the survey was accepted in 38 SME’s. In slightly more than half of them a sick leave related policy has been defined. While almost all SME’s have a well-defined procedure for the RTW examination by the OP, only 22 (out of 38) have defined procedures for maintaining a contact with the worker during the sick leave period, only 17 are informing their employees about the possibility to meet the OP during that period, and only 6 are systematically organising a worker-supervisor meeting when the worker comes back at work. Knowledge about RTW regulations is rather poor: 10 managers wrongly think that the OP may check the sick leave medical validity; only 14 managers know that the employer must inform the OP about any sick leave longer than 4 weeks; less than half of the managers have a good knowledge of the pre-return to work visit. Various expectations have been put forward as regard the role of the OH service: knowledge transfer about the regulations, coaching of the supervisors, training managers in carrying RTW talks with the worker, suggesting procedures, etc. Conclusions The lack of knowledge about RTW regulations underlines the need for information campaigns focused on the employers. The participating RH managers are awaiting a more proactive role from their OH service. [less ▲]

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