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See detailIn Vitro Activities of Strychnos Alkaloids and Extracts against Plasmodium Falciparum
Frederich, Michel ULg; Hayette, Marie-Pierre ULg; Tits, Monique ULg et al

in Antimicrobial Agents and Chemotherapy (1999), 43(9), 2328-31

The in vitro antimalarial activities of 46 alkaloids and extracts from Strychnos species were evaluated. Two types of quasidimeric alkaloids exhibit high and selective activities against Plasmodium ... [more ▼]

The in vitro antimalarial activities of 46 alkaloids and extracts from Strychnos species were evaluated. Two types of quasidimeric alkaloids exhibit high and selective activities against Plasmodium. Strychnopentamine and isostrychnopentamine were active against chloroquine-sensitive and -resistant strains (50% inhibitory concentration [IC(50)] approximately 0.15 microM), while dihydrousambarensine exhibited a 30-fold higher activity against the chloroquine-resistant strain (IC(50) = 0.03 microM) than it did against the chloroquine-sensitive strain. [less ▲]

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See detailIn vitro activity of amoxycillin plus clavulanic acid and ticarcillin plus clavulanic acid compared with that of other antibiotics against anaerobic bacteria
Pierard, Denis; De Meyer, A.; Rossell, P. et al

in Acta Clinica Belgica (1989), 44(4), 228-36

The activity of amoxycillin/clavulanic acid (Augmentin) and ticarcillin/clavulanic acid (Timentin) was tested against 303 unselected clinical anaerobic isolates recently collected in seven Belgian ... [more ▼]

The activity of amoxycillin/clavulanic acid (Augmentin) and ticarcillin/clavulanic acid (Timentin) was tested against 303 unselected clinical anaerobic isolates recently collected in seven Belgian university hospitals and compared with that of 11 other antimicrobial agents. Bacteroides spp. accounted for 52.1% of the isolates, Clostridium spp. for 23.4%, anaerobic cocci for 15.5%, nonsporeforming gram-positive bacilli for 4.6% and Fusobacterium spp. for 3.3%. Ticarcillin/clavulanic acid (fixed clavulanic acid concentration of 2 mg/l) was the most active drug with an overall susceptibility rate of 99.7%. Amoxycillin/clavulanic acid (fixed ratio of 2:1) and chloramphenicol inhibited 97.4% of the isolates, metronidazole 95.4%, piperacillin 92.4%, ticarcillin 91.4%, clindamycin 87.8%, cefotetan 81.2%, cefazolin 63.0%, cefuroxime 60.4%, erythromycin 57.8%, penicillin 57.1% and doxycycline 52.1%. beta-lactamases were detected exclusively in Bacteroides spp. isolates (79.1% positive). [less ▲]

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See detailIn vitro activity of amoxycillin/clavulanate and ticarcillin/clavulanate compared with that of other antibiotics against anaerobic bacteria: comparison with the results of the 1987 survey
Pierard, denis; De Meyer A; Rosseel, P. et al

in Acta Clinica Belgica (1996), 51(2), 70-9

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight ... [more ▼]

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight Belgian university hospitals and compared with that of 8 other antibiotics using the NCCLS reference agar dilution procedure. Production of beta-lactamase was detected by the nitrocefin test in 48% of the isolates. At NCCLS-recommended breakpoints, more than 90% of isolates were susceptible to amoxycillin/clavulanate, ticarcillin/clavulanate, piperacillin/tazobactam, imipenem, chloramphenicol and metronidazole but only 77%, 72% and 48% to cefoxitin, clindamycin and penicillin, respectively. In comparison with the results of a similar survey conducted in 1987 no major changes in susceptibility were observed except for the susceptibility to clindamycin that declined from 83% to 72% overall, and from 83% to 66% in the B. fragilis group. Furthermore one isolate of Clostridium clostridioforme was found produce beta-lactamase and few B. fragilis group isolates showed reduced susceptibility to metronidazole. [less ▲]

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See detailIn vitro activity of Beauveria bassiana against Psoroptes ovis
Lekimme, M.; Mignon, Bernard ULg; Focant, C. et al

Poster (2005)

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See detailIn vitro and in situ experimental modulation of neurotransmitters, phenotypic expression by adult dorsal root ganglion neurons
Schoenen, J.; Delrée, P.; Martin, Didier ULg et al

Conference (1989, November)

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See detailIn Vitro And In Situ Study Of Postharvest Apple Blue Mold Biocontrol By Aureobasidium Pullulans: Evidence For The Involvement Of Competition For Nutrients
Bencheqroun, Sk.; Baji, M.; Massart, Sébastien ULg et al

in Postharvest Biology and Technology (2007), 46(2), 128-135

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See detailIn vitro and in vivo activity of the nuclear factor-kappa B inhibitor sulfasalazine in human glioblastomas.
Robe, Pierre ULg; Bentires-Alj, Mohamed; Bonif, Marianne et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (2004), 10(16), 5595-603

Glioblastomas, the most common primary brain cancers, respond poorly to current treatment modalities and carry a dismal prognosis. In this study, we demonstrated that the transcription factor nuclear ... [more ▼]

Glioblastomas, the most common primary brain cancers, respond poorly to current treatment modalities and carry a dismal prognosis. In this study, we demonstrated that the transcription factor nuclear factor (NF)-kappaB is constitutively activated in glioblastoma surgical samples, primary cultures, and cell lines and promotes their growth and survival. Sulfasalazine, an anti-inflammatory drug that specifically inhibits the activation of NF-kappaB, blocked the cell cycle and induced apoptosis in several glioblastoma cell lines and primary cultures, as did gene therapy with a vector encoding a super-repressor of NF-kappaB. In vivo, sulfasalazine also significantly inhibited the growth of experimental human glioblastomas in nude mice brains. Given the documented safety of sulfasalazine in humans, these results may lead the way to a new class of glioma treatment. [less ▲]

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See detailIn vitro and in vivo analysis of macroporous biodegradable poly(D,L-lactide-co-glycolide) scaffolds containing bioactive glass
Day, Richard M; Maquet, Véronique; Boccaccini, Aldo R. et al

in Journal of Biomedical Materials Research, Part A (2005), 75A(4), 778-787

Recent studies have demonstrated the angiogenic potential of 45S5 Bioglass (R). However, it is not known whether the angiogenic properties of Bioglass (R) remain when the bioactive glass particles are ... [more ▼]

Recent studies have demonstrated the angiogenic potential of 45S5 Bioglass (R). However, it is not known whether the angiogenic properties of Bioglass (R) remain when the bioactive glass particles are incorporated into polymer composites. The objectives of the current study were to investigate the angiogenic properties of 45S5 Bioglass (R) particles incorporated into biodegradable polymer composites. In vitro studies demonstrated that fibroblasts Cultured on discs consisting of specific quantities of Bioglass (R) particles mixed into poly(D,L-lactide-co-glycolide) secreted significantly increased quantities of vascular endothelial growth factor. The optimal quantity of Bioglass (R) particles determined from the in vitro experiments was incorporated into three-dimensional macroporous poly(D,L-lactide-co-glycolide) foam scaffolds. The foam scaffolds were fabricated using either compression molding or thermally induced phase separation processes. The foams were implanted subcutaneously into mice for periods Of Lip to 6 weeks. Histological assessment was used to determine the area of granulation tissue around the foams, and the number of blood vessels within the granulation tissue was counted. The presence of Bioglass (R) particles in the foams produced a sustained increase in the area of granulation tissue surrounding the foams. The number of blood vessels surrounding the neat foams was reduced after 2 weeks of implantation; however, compression-molded foams containing Bioglass (R) after 4 and 6 weeks of implantation had significant]), more blood vessels surrounding the foams compared with foams containing no Bioglass (R) at the same time points. These results indicate that composite polymer foam scaffolds containing Bioglass (R) particles retain granulation tissue and blood vessels surrounding the implanted foams. The use of this polymer composite for tissue engineering scaffolds might provide a novel approach for ensuring adequate vascular Supply to the implanted device. [less ▲]

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See detailIn vitro and in vivo antimalarial and cytotoxic activity of five plants used in Congolese traditional medicine.
Lusakibanza, M.; Mesia, G.; Tona, G. et al

in Journal of Ethnopharmacology (2010), 129

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ... [more ▼]

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. MATERIALS AND METHODS: The growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH(3)OH, EtOH and CH(2)Cl(2)) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. RESULTS: Two plants presented a very high activity (IC(50)<3mug/ml). These plants were Strychnos icaja roots bark (MeOH and CH(2)Cl(2)) and Physalis angulata leaves (MeOH and CH(2)Cl(2)). One plant (Anisopappus chinensis whole plant, MeOH and CH(2)Cl(2)) presented a high activity (IC50<15mug/ml). The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. CONCLUSION: Three plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata and Strychnos icaja) and one of them showed a good selectivity index (>10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo. [less ▲]

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See detailIn vitro and in vivo antimalarial properties of isostrychnopentamine, an indolomonoterpenic alkaloid from Strychnos usambarensis
Frederich, Michel ULg; Tits, Monique ULg; Goffin, Eric ULg et al

in Planta Medica (2004), 70(6), 520-525

Isostrychnopentamine (ISP) is an asymmetric indolomonoterpenic alkaloid isolated from the leaves of Strychnos usambarensis. The in vitro antiplasmodial activities against five Plasmodium falciparum cell ... [more ▼]

Isostrychnopentamine (ISP) is an asymmetric indolomonoterpenic alkaloid isolated from the leaves of Strychnos usambarensis. The in vitro antiplasmodial activities against five Plasmodium falciparum cell lines were evaluated: ISP possessed an in vitro IC (50) near 0.1 microM against all P. falciparum cell lines tested (chloroquine-resistant and chloroquine-sensitive lines) and showed antiplasmodial selectivity compared to cytotoxicity on human cell lines. The stage-dependent susceptibility to a short exposure of ISP was then investigated. The ring stage was shown to be the most sensitive one, but all stages were affected by ISP treatment. By means of fluorescence microscopy, it was shown that ISP was not accumulated inside the food vacuole of the parasite. Finally, the in vivo antimalarial activities against the P. berghei NK173 and P. vinckei petteri murine strains were determined. The ED (50) in vivo was about 30 mg/kg/day by the intraperitoneal route (after 4 days treatment). [less ▲]

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See detailIn Vitro and In Vivo Antiplasmodial Activity of Three Rwandan Medicinal Plants and Identification of Their Active Compounds
Muganga, Raymond; Angenot, Luc ULg; Tits, Monique ULg et al

in Planta Medica (2014), 80(6), 482-489

In our previous study, we reported the interesting in vitro antiplasmodial activity of some Rwandan plant extracts. This gave rise to the need for these extracts to also be evaluated in vivo and to ... [more ▼]

In our previous study, we reported the interesting in vitro antiplasmodial activity of some Rwandan plant extracts. This gave rise to the need for these extracts to also be evaluated in vivo and to identify the compounds responsible for their antiplasmodial activity. The aim of our study was, on the one hand, to evaluate the antiplasmodial activity in vivo and the safety of the selected Rwandan medicinal plants used in the treatment of malaria, with the objective of promoting the development of improved traditional medicines and, on the other hand, to identify the active ingredients in the plants. Plant extracts were selected according to their selectivity index. The in vivo antiplasmodial activity of aqueous, methanolic, and dichloromethane extracts was then evaluated using the classical 4-day suppressive test on Plasmodium berghei infected mice. The activity of the plant extracts was estimated by measuring the percentage of parasitemia reduction, and the survival of the experimental animals was recorded. A bioguided fractionation was performed for the most promising plants, in terms of antiplasmodial activity, in order to isolate active compounds identified by means of spectroscopic and spectrometric methods. The highest level of antiplasmodial activity was observed with the methanolic extract of Fuerstia africana (> 70 %) on days 4 and 7 post-treatment after intraperitoneal injection and on day 7 using oral administration. After oral administration, the level of parasitemia reduction observed on day 4 post-infection was 44 % and 37 % with the aqueous extract of Terminalia mollis and Zanthoxylum chalybeum, respectively. However, the Z. chalybeum extract presented a high level of toxicity after intraperitoneal injection, with no animals surviving on day 1 post-treatment. F. africana, on the other hand, was safer with 40 % mouse survival on day 20 post-treatment. Ferruginol is already known as the active ingredient in F. Africana, and ellagic acid (IC50 = 175 ng/mL) and nitidine (IC50 = 77.5 ng/mL) were identified as the main active constituents of T. mollis and Z. chalybeum, respectively. F. africana presented very promising antiplasmodial activity in vivo. Although most of the plants tested showed some level of antiplasmodial activity, some of these plants may be toxic. This study revealed for the first time the role of ellagic acid and nitidine as the main antimalarial compounds in T. mollis and Z. chalybeum, respectively. [less ▲]

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See detailThe In Vitro and In Vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer
Jamison, james; Neal, Deborah; Getch, Steve et al

in Lucas, John N (Ed.) Horizons cancer Res. , Trends in prostate cancer research (2005)

Neoplasms of the urinary tract and male genital tract account for 32% of new cancers in males. Prostatic carcinoma is one of the most prevalent malignant tumors of the male reproductive organs, with an ... [more ▼]

Neoplasms of the urinary tract and male genital tract account for 32% of new cancers in males. Prostatic carcinoma is one of the most prevalent malignant tumors of the male reproductive organs, with an estimated 230,110 new cases and 29,900 deaths during the year 2004 in the United States. Although prostate cancer in its early stages is responsive to the standard treatments, no currently available treatment produces a survival advantage to patients with hormone-refractory prostate cancer. This lack of efficacy for the existing protocols points to the need for the development of effective regimens for treating or preventing these tumors. Vitamin C (VC) and vitamin K3 (VK3) administered to androgen independent prostate cancer cell lines in a VC:VK3 ratio of 100:1 exhibit synergistic antitumor activity and preferentially kill tumor cells by autoschizis, a novel type of cell death characterized by exaggerated membrane damage and progressive loss of organelle-free cytoplasm through a series of self-excisions. During this process, the nucleus becomes smaller; cell size decreases to 1/2 - 1/3 of its original size and most organelles surround an intact nucleus in a narrow rim of cytoplasm. While the mitochondria are condensed, tumor cell death does not result from ATP depletion. However, administration of the vitamin combination does: induce a G1/S and a G2/M block in the cell cycle, diminish DNA synthesis, increase hydrogen peroxide (H2O2) production and decrease cellular thiol levels. These effects can be prevented by the addition of catalase to scavenge the H2O2. There is a concurrent 8- to 10-fold increase in intracellular Ca2+ levels. Electrophoretic analysis of DNA reveals degradation due to the caspase-3 independent reactivation of DNase I by VK3 and DNase II by VC. Redox cycling of the vitamins is believed to increase oxidative stress until it surpasses the reducing ability of cellular thiols and induces Ca2+ release which triggers activation of Ca2+ dependent DNase and leads to degradation of DNA. Recent experiments indicate oral VC :VK3 increases the life span of tumor bearing nude mice and significantly reduces the growth rate of solid tumors without any significant toxicity by reactivating DNase I and II. Experimental animal studies indicate that autoschizis is also evident when the vitamin combination is administered to nude mice with implanted human prostate tumors. In a pilot clinical trial, end-stage prostate cancer patients given oral VC:VK3 are monitored by measuring prostate-specific antigen (PSA, a known serum marker of prostate tumor cells specific activity) and total serum homocysteine (a marker of tumor cell numbers with sensitivity for early detection of tumor cell death). The results show that the vitamin combination induces an immediate drop in tumor cell numbers, while serum PSA levels show an initial rise and a subsequent drop to below pretreatment levels. Taken together these facts suggest that the vitamin combination may offer great potential as a new antitumoral chemotherapy. The following chapter discusses the mechanisms of action employed by these vitamins to induce tumor specific death by autoschizis and examines the potential of the vitamin combination as an antitumor agent, chemosensitizer or a radiosensitizer in the treatment of prostatic neoplasms. [less ▲]

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See detailIn vitro and in vivo Characterization of Adult Bone Marrow Neural Crest Stem Cells
Coste, Cécile ULg; Neirinckx, Virginie ULg; Manguette, Jérôme et al

Poster (2013, May 31)

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See detailIn Vitro and In Vivo Characterization of Plant Growth Promoting Bacillus Strains Isolated from Extreme Environments of Eastern Algeria.
Ait-Kaki, Asma; Kacem-Chaouche, Noreddine; Ongena, Marc ULg et al

in Applied biochemistry and biotechnology (2014), 172

This report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of Bacillus isolates from extreme environments of Eastern Algeria. Seven isolates of 14 (50 %) were ... [more ▼]

This report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of Bacillus isolates from extreme environments of Eastern Algeria. Seven isolates of 14 (50 %) were screened for their ability to inhibit growth of some phytopathogenic fungi on PDA and some roots exudates. The bacteria identification based on 16S r-RNA and gyrase-A gene sequence analysis showed that 71 % of the screened isolates belonged to Bacillus amyloliquefaciens and the rest were closely related to B. atrophaeus and B. mojavensis. Most of them had high spore yields (22 x 108-27 x 108 spores/ml). They produced protease and cellulase cell wall-degrading enzymes while the chitinase activity was only observed in the B. atrophaeus (6SEL). A wide variety of lipopeptides homologous was detected by liquid chromatography-electrospray ionization-mass spectrometry analysis. Interestingly, some additional peaks with new masses were characterized, which may correspond to new fengycin classes. The isolates produced siderophores and indole-3- acetic acid phytohormone. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. atrophaeus (6SEL) significantly increased the size of the chickpea plants and reduced the stem rot disease (P < 0.05). These results suggest that these isolates may be used further as bio-inoculants to improve crop systems. [less ▲]

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