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See detailIn Vitro and In Vivo Antiplasmodial Activity of Three Rwandan Medicinal Plants and Identification of Their Active Compounds
Muganga, Raymond; Angenot, Luc ULg; Tits, Monique ULg et al

in Planta Medica (2014), 80(6), 482-489

In our previous study, we reported the interesting in vitro antiplasmodial activity of some Rwandan plant extracts. This gave rise to the need for these extracts to also be evaluated in vivo and to ... [more ▼]

In our previous study, we reported the interesting in vitro antiplasmodial activity of some Rwandan plant extracts. This gave rise to the need for these extracts to also be evaluated in vivo and to identify the compounds responsible for their antiplasmodial activity. The aim of our study was, on the one hand, to evaluate the antiplasmodial activity in vivo and the safety of the selected Rwandan medicinal plants used in the treatment of malaria, with the objective of promoting the development of improved traditional medicines and, on the other hand, to identify the active ingredients in the plants. Plant extracts were selected according to their selectivity index. The in vivo antiplasmodial activity of aqueous, methanolic, and dichloromethane extracts was then evaluated using the classical 4-day suppressive test on Plasmodium berghei infected mice. The activity of the plant extracts was estimated by measuring the percentage of parasitemia reduction, and the survival of the experimental animals was recorded. A bioguided fractionation was performed for the most promising plants, in terms of antiplasmodial activity, in order to isolate active compounds identified by means of spectroscopic and spectrometric methods. The highest level of antiplasmodial activity was observed with the methanolic extract of Fuerstia africana (> 70 %) on days 4 and 7 post-treatment after intraperitoneal injection and on day 7 using oral administration. After oral administration, the level of parasitemia reduction observed on day 4 post-infection was 44 % and 37 % with the aqueous extract of Terminalia mollis and Zanthoxylum chalybeum, respectively. However, the Z. chalybeum extract presented a high level of toxicity after intraperitoneal injection, with no animals surviving on day 1 post-treatment. F. africana, on the other hand, was safer with 40 % mouse survival on day 20 post-treatment. Ferruginol is already known as the active ingredient in F. Africana, and ellagic acid (IC50 = 175 ng/mL) and nitidine (IC50 = 77.5 ng/mL) were identified as the main active constituents of T. mollis and Z. chalybeum, respectively. F. africana presented very promising antiplasmodial activity in vivo. Although most of the plants tested showed some level of antiplasmodial activity, some of these plants may be toxic. This study revealed for the first time the role of ellagic acid and nitidine as the main antimalarial compounds in T. mollis and Z. chalybeum, respectively. [less ▲]

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See detailThe In Vitro and In Vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer
Jamison, james; Neal, Deborah; Getch, Steve et al

in Lucas, John N (Ed.) Horizons cancer Res. , Trends in prostate cancer research (2005)

Neoplasms of the urinary tract and male genital tract account for 32% of new cancers in males. Prostatic carcinoma is one of the most prevalent malignant tumors of the male reproductive organs, with an ... [more ▼]

Neoplasms of the urinary tract and male genital tract account for 32% of new cancers in males. Prostatic carcinoma is one of the most prevalent malignant tumors of the male reproductive organs, with an estimated 230,110 new cases and 29,900 deaths during the year 2004 in the United States. Although prostate cancer in its early stages is responsive to the standard treatments, no currently available treatment produces a survival advantage to patients with hormone-refractory prostate cancer. This lack of efficacy for the existing protocols points to the need for the development of effective regimens for treating or preventing these tumors. Vitamin C (VC) and vitamin K3 (VK3) administered to androgen independent prostate cancer cell lines in a VC:VK3 ratio of 100:1 exhibit synergistic antitumor activity and preferentially kill tumor cells by autoschizis, a novel type of cell death characterized by exaggerated membrane damage and progressive loss of organelle-free cytoplasm through a series of self-excisions. During this process, the nucleus becomes smaller; cell size decreases to 1/2 - 1/3 of its original size and most organelles surround an intact nucleus in a narrow rim of cytoplasm. While the mitochondria are condensed, tumor cell death does not result from ATP depletion. However, administration of the vitamin combination does: induce a G1/S and a G2/M block in the cell cycle, diminish DNA synthesis, increase hydrogen peroxide (H2O2) production and decrease cellular thiol levels. These effects can be prevented by the addition of catalase to scavenge the H2O2. There is a concurrent 8- to 10-fold increase in intracellular Ca2+ levels. Electrophoretic analysis of DNA reveals degradation due to the caspase-3 independent reactivation of DNase I by VK3 and DNase II by VC. Redox cycling of the vitamins is believed to increase oxidative stress until it surpasses the reducing ability of cellular thiols and induces Ca2+ release which triggers activation of Ca2+ dependent DNase and leads to degradation of DNA. Recent experiments indicate oral VC :VK3 increases the life span of tumor bearing nude mice and significantly reduces the growth rate of solid tumors without any significant toxicity by reactivating DNase I and II. Experimental animal studies indicate that autoschizis is also evident when the vitamin combination is administered to nude mice with implanted human prostate tumors. In a pilot clinical trial, end-stage prostate cancer patients given oral VC:VK3 are monitored by measuring prostate-specific antigen (PSA, a known serum marker of prostate tumor cells specific activity) and total serum homocysteine (a marker of tumor cell numbers with sensitivity for early detection of tumor cell death). The results show that the vitamin combination induces an immediate drop in tumor cell numbers, while serum PSA levels show an initial rise and a subsequent drop to below pretreatment levels. Taken together these facts suggest that the vitamin combination may offer great potential as a new antitumoral chemotherapy. The following chapter discusses the mechanisms of action employed by these vitamins to induce tumor specific death by autoschizis and examines the potential of the vitamin combination as an antitumor agent, chemosensitizer or a radiosensitizer in the treatment of prostatic neoplasms. [less ▲]

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See detailIn vitro and in vivo Characterization of Adult Bone Marrow Neural Crest Stem Cells
Coste, Cécile ULg; Neirinckx, Virginie ULg; Manguette, Jérôme et al

Poster (2013, May 31)

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See detailIn Vitro and In Vivo Characterization of Plant Growth Promoting Bacillus Strains Isolated from Extreme Environments of Eastern Algeria.
Ait-Kaki, Asma; Kacem-Chaouche, Noreddine; Ongena, Marc ULg et al

in Applied biochemistry and biotechnology (2014), 172

This report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of Bacillus isolates from extreme environments of Eastern Algeria. Seven isolates of 14 (50 %) were ... [more ▼]

This report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of Bacillus isolates from extreme environments of Eastern Algeria. Seven isolates of 14 (50 %) were screened for their ability to inhibit growth of some phytopathogenic fungi on PDA and some roots exudates. The bacteria identification based on 16S r-RNA and gyrase-A gene sequence analysis showed that 71 % of the screened isolates belonged to Bacillus amyloliquefaciens and the rest were closely related to B. atrophaeus and B. mojavensis. Most of them had high spore yields (22 x 108-27 x 108 spores/ml). They produced protease and cellulase cell wall-degrading enzymes while the chitinase activity was only observed in the B. atrophaeus (6SEL). A wide variety of lipopeptides homologous was detected by liquid chromatography-electrospray ionization-mass spectrometry analysis. Interestingly, some additional peaks with new masses were characterized, which may correspond to new fengycin classes. The isolates produced siderophores and indole-3- acetic acid phytohormone. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. atrophaeus (6SEL) significantly increased the size of the chickpea plants and reduced the stem rot disease (P < 0.05). These results suggest that these isolates may be used further as bio-inoculants to improve crop systems. [less ▲]

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See detailIn vitro and in vivo comparative study of material properties crucial for reverse engineering of calcium phosphate scaffolds
Chai, Yoke Chin; Kerckhofs, Greet ULg; Bertels, Jeroen et al

Conference (2013)

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See detailIn vitro and in vivo effects of new insulin releasing agents
Nguyen, Q. A.; Antoine, M. H.; Ouedraogo, R. et al

in Biochemical Pharmacology (2002)

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See detailIn vitro and in vivo effects of salbutamol acetonide on cat airways
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, F. et al

in Proceedings: Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques (2005)

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See detailIn vitro and in vivo evaluation of [I-123]-VEGF(165) as a potential tumor marker
Cornelissen, B.; Oltenfreiter, R.; Kersemans, V. et al

in Nuclear Medicine & Biology (2005), 32(5), 431-436

One of the research challenges in oncology is to develop new biochemical methods for noninvasive tumor therapy evaluation to determine,whether the chemotherapeutics is effective. Vascular endothelial ... [more ▼]

One of the research challenges in oncology is to develop new biochemical methods for noninvasive tumor therapy evaluation to determine,whether the chemotherapeutics is effective. Vascular endothelial growth factor (VEGF) was labeled with radioiodine and evaluated in vitro as well as in vivo, using A2058, a melanoma cell line overexpressing VEGFR-1 and -2. Saturation binding analysis with [I-125]-VEGF resulted in a K-d of 0.1 nM. Internalization assays indicate the preserved ligand induced internalization and metabolization of the tracer. Biodistribution studies with [I-123]-VEGF in wild type and A2058 tumor-bearing athymic mice showed low background activity and a tumor to reference tissue ratio of maximum 6.12. These results suggest that [I-123]-VEGF is a potentially suitable tracer for tumor therapy evaluation. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailIn vitro and in vivo models of varicella-zoster virus persistence in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Journal of Cellular Biochemistry (1988), 12C

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See detailIn Vitro and in Vivo Modulation of 5-Hydroxytryptamine-, Thyrotropin-Releasing Hormone- and Calcitonin-Gene Related Peptide-Like Immunoreactivities in Adult Rat Sensory Neurons
Delree, P.; Martin, Didier ULg; Sadzot-Delvaux, Catherine ULg et al

in Neuroscience (1992), 51(2), 401-10

In a previous work we have shown that culturing adult rat dorsal root ganglia neurons modifies their neurotransmitter phenotype in such a way that cultured neurons synthesize transmitters that are not ... [more ▼]

In a previous work we have shown that culturing adult rat dorsal root ganglia neurons modifies their neurotransmitter phenotype in such a way that cultured neurons synthesize transmitters that are not found in situ, while several other transmitters are expressed in a much higher percentage of neurons in culture than in situ [Schoenen J. et al. (1989) J. Neurosci. Res. 22, 473-487]. The aim of the present study was to investigate the origin and the nature of the relevant environmental signals that allow this plasticity to be expressed, focusing on three neurotransmitters: 5-hydroxytryptamine, thyrotropin-releasing hormone and calcitonin-gene related peptide. The main results can be summarized as follows: (1) culturing cells in fetal calf serum or on feeder layers of astrocytes, Schwann cells or fibroblasts partially inhibits the serotoninergic phenotype of dorsal root ganglia neurons; (2) in vivo disconnection of dorsal root ganglia from their spinal targets but not from their peripheral or supraspinal targets induces a significant increase of the percentage of 5-hydroxytryptamine- and thyrotropin-releasing hormone-positive neurons in disconnected ganglia; (3) growth factors such as ciliary neuronotrophic factor or basic fibroblast growth factor but not nerve growth factor repress 5-hydroxytryptamine and calcitonin gene-related peptide immunoreactivity in cultured sensory neurons. In conclusion, neurotransmitter gene expression of adult dorsal root ganglia neurons is controlled by complex influences. Our data suggest that thyrotropin-releasing hormone and 5-hydroxytryptamine gene expression are tonically repressed in vivo by factors originating from the spinal segmental level and that growth factors such as ciliary neurotrophic factor or basic fibroblast growth factor could be potential vectors of this repressing effect. [less ▲]

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See detailIn vitro and in vivo modulation of neurotransmitter phenotype in adult dorsal root ganglion neurons.
Schoenen, Jean ULg; Delrée, P.; Jammaer, R. et al

Conference (1990, June 16)

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See detailIn vitro and in vivo modulation of neurotransmitter phenotype in adult DRG neuron.
Schoenen, Jean ULg; Delrée, P.; Martin, Didier ULg et al

Conference (1990)

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See detailIn vitro and in vivo modulation of neurotransmitter phenotype in adult rat DRG neurons
Schoenen, Jean ULg; Delrée, P.; Martin, Didier ULg et al

in Rapport annuel de la Fondation Médicale Reine Elisabeth (1990)

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See detailIn vitro and in vivo modulation of transmitter phenotype in adult rat DRG neurons.
Moonen, Gustave ULg; Schoenen, Jean ULg; Delrée, P. et al

Conference (1991, August 11)

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See detailIn Vitro And In Vivo Oncogenic Potential Of Bovine Leukemia Virus G4 Protein
Kerkhofs, P.; Heremans, H.; Burny, A. et al

in Journal of Virology (1998), 72(3),

Detailed reference viewed: 4 (1 ULg)