How to monitor NF-kappaB activation after photodynamic therapy.
Coupienne, Isabelle ; Piette, Jacques ; Bontems, Sébastien
in Methods in Molecular Biology (Clifton, N.J.) (2010), 635
The nuclear factor-kappa B (NF-kappaB) is a multipotent factor involved in many cellular processes such as inflammation, immune response and embryonic development and it can be activated by a large number ... [more ▼]
The nuclear factor-kappa B (NF-kappaB) is a multipotent factor involved in many cellular processes such as inflammation, immune response and embryonic development and it can be activated by a large number of stimuli. Consequently, this transcription factor plays a pivotal role in many natural processes but also in different pathologies. For several years, photodynamic therapy (PDT) has emerged as an attractive alternative approach for the treatment of different affections involving various forms of cancer and an increasing number of reports have highlighted the activation of the NF-kappaB following PDT treatment. Furthermore, it has been shown that the mechanism of activation of the NF-kappaB as well as its target genes depends on the nature of the photosensitizers and the cell type used. As this transcription factor is known to be a key regulator of the immune response but also controls cell survival and proliferation, it is important to assess its activation status and its impact on the target genes. In this review, we will present different techniques allowing identification of the activation status of this factor, from the degradation of its inhibitor in the cytoplasm to its ability to induce the expression of a reporter gene under the control of a target promoter. As a working model we will present results obtained from a 5-aminolevulinic acid-PDT treatment on cervix adenocarcinoma cells. [less ▲]Detailed reference viewed: 43 (22 ULg)
How to optimally load a set of containers into an aircraft
Schyns, Michael ; Limbourg, Sabine ;
E-print/Working paper (2012)Detailed reference viewed: 63 (10 ULg)
How to optimize physicians' communication skills in cancer care: Results of a randomized study assessing the usefulness of posttraining consolidation workshops
; ; Marchal, Sylvie et al
in Journal of Clinical Oncology (2003), 21(16), 3141-3149
Purpose: Although there is wide recognition of the usefulness of improving physicians' communication skills, no studies have yet assessed the efficacy of post-training consolidation workshops. This study ... [more ▼]
Purpose: Although there is wide recognition of the usefulness of improving physicians' communication skills, no studies have yet assessed the efficacy of post-training consolidation workshops. This study aims to assess the efficacy of six 3-hour consolidation workshops conducted after a 2.5-day basic training program. Methods: Physicians, after attending the basic training program, were randomly assigned to consolidation workshops or to a waiting list. Training efficacy was assessed through simulated and actual patient interviews that were audiotaped at baseline and after consolidation workshops for the consolidation-workshop group, and approximately 5 months after the end of basic training for the waiting-list group. Communication skills were assessed according to the Cancer Research Campaign Workshop Evaluation Manual. Patients' perceptions of communication skills improvement were assessed using a 14-item questionnaire. Results : Sixty-three physicians completed the training program. Communication skills improved significantly more in the consolidation-workshop group compared with the waiting-list group. In simulated interviews, group-by-time repeated measures analysis of variance showed a significant increase in open and open directive questions (P =.014) and utterances alerting patients to reality (P =.049), as well as a significant decrease in premature reassurance (P =.042). In actual patient interviews, results revealed a significant increase in acknowledgments (P =.022) and empathic statements (P =.009), in educated guesses (P =.041), and in negotiations (P =.008). Patients interacting with physicians who benefited from consolidation workshops reported higher scores concerning their physicians' understanding of their disease (P =.004). Conclusion: Consolidation workshops further improve a communication skills training program's efficacy and facilitate the transfer of acquired skills to clinical practice. [less ▲]Detailed reference viewed: 24 (0 ULg)
How to perform the diagnosis in a case with anemia ?
in Amory, Hélène (Ed.) In proceedings of the II Encontro de Formação da Ordem dos Médicos Veterinários (2011, October 08)Detailed reference viewed: 21 (0 ULg)
How to perform the diagnosis in a case with icterus ?
in In proceedings of the II Encontro de Formação da Ordem dos Médicos Veterinários (2011, October 08)Detailed reference viewed: 21 (0 ULg)
How to perform the most minimal invasive cholecystectomy?
Conference (2013, September 20)
Twenty years ago, laparoscopy has totally transformed the surgical approach of the gallbladder. For those who were not in surgery at that time, it has to be reminded that most open cholecystectomies ... [more ▼]
Twenty years ago, laparoscopy has totally transformed the surgical approach of the gallbladder. For those who were not in surgery at that time, it has to be reminded that most open cholecystectomies performed in the 80’s required nasogastric tube for several days, surgical drain, and 5 to 8 days of hospitalisation. For these obvious advantages, laparoscopic cholecystectomy has become the “Gold Standard” without evidence by a randomised controlled study comparing open and laparoscopic techniques. These last years, some groups proposed variations of laparoscopic cholecystectomies that they presented as less invasive. In robotic surgery, the Da-Vinci device has been proposed without significant improvement since more than 10 years. To date, no interest of this Da-Vinci robot has been demonstrated in abdominal surgery. Its cost for acquisition, its yearly fee and the cost for each use render its use without proof of results totally inappropriate. Single-Incision Laparoscopic Surgery (SILS) has also been described to perform cholecystectomy with only one larger umbilical incision. Until now, no superiority of SILS on classical cholecystectomy has been demonstrated. The Natural Orifice Transluminal Endoscopic Surgery (NOTES) proposes to use the vagina, the stomach or the colon to remove the gallbladder. No prospective controlled comparison has proved the superiority of these approaches that sometimes do not reach common sense or usual surgical principles. What is a minimal invasive cholecystectomy? A safe and un expensive cholecystectomy, with a good anatomical exposure, a cholangiography, and a good surgical comfort allowing to perform several procedures in the same day; a cholecystectomy that can be performed as a day-case without complications; a cholecystectomy that can be performed laparoscopically in acute settings; a cholecystectomy without the risk of postoperative incisional hernia. In necessary, in young and thin woman, laparoscopic cholecystectomy may be performed using 2 mm trocars that allow a good triangulation but with minimal scars. [less ▲]Detailed reference viewed: 25 (2 ULg)
How to Portray Customers Using Collaborative Database Profiling: Applications to Age and Political Partisanship Estimations
; Tramonte, Nathalie
Conference (2008, June)Detailed reference viewed: 30 (4 ULg)
How to prescribe parenteral nutrition in clinical difficulties
in Congress of the Portuguese Neonatal Society National (2012)Detailed reference viewed: 1 (0 ULg)
How to prevent bankruptcy in Belgian SMEs ? Towards the implementation of an effective process to detect and assist failing SMEs
Van Caillie, Didier
Conference given outside the academic context (2006)
This conference discusses why it's crucial, in an open economy such as the Belgian one, to prevent business failure in SMEs and to assist entrepreneurs to overcome their difficulties. It insists on the ... [more ▼]
This conference discusses why it's crucial, in an open economy such as the Belgian one, to prevent business failure in SMEs and to assist entrepreneurs to overcome their difficulties. It insists on the role of Public Authorities in assisting entrepreneurs to detect and correct as soon as possible the potential causes of failure. [less ▲]Detailed reference viewed: 42 (1 ULg)
How to rapidly increase the overproduction of a recombinant enzyme?
; ; et al
Poster (2001)Detailed reference viewed: 5 (0 ULg)
How to remain a 'father' in times of privation? Respect, reciprocity and morality in Gécamines families (Katanga, D.R. Congo)
Conference (2012)Detailed reference viewed: 12 (2 ULg)
How to remain a father in times of privation? Respect and reciprocity in the families of Gécamines workers, D.R.Congo
Conference (2010)Detailed reference viewed: 21 (2 ULg)
How to remain nonfolded and pliable: the linkers in modular alpha-amylases as a case study
Feller, Georges ; Dehareng, Dominique ;
in FEBS Journal (2011), 278Detailed reference viewed: 14 (6 ULg)
How to remove clouds in a time series of SST images?: Application to the Canary Island - Madeira Region
Troupin, Charles ; Alvera Azcarate, Aïda ; Barth, Alexander et al
Conference (2012, November 16)Detailed reference viewed: 30 (3 ULg)
How to reveal the amazing soil microbial diversity ?
Scientific conference (2014, May 22)Detailed reference viewed: 11 (2 ULg)
How to simulate quickly and efficiently a flow over a spillway ?
Goffin, Louis ; Erpicum, Sébastien ; Dewals, Benjamin et al
Conference (2014, August)
Flows over hydraulic structures, such as weirs or spillways, can be modelled using different techniques. New models such as SPH or PFEM are becoming more and more popular. These models are particle and/or ... [more ▼]
Flows over hydraulic structures, such as weirs or spillways, can be modelled using different techniques. New models such as SPH or PFEM are becoming more and more popular. These models are particle and/or meshless and consequently require a lot of computational power. Other methods such as VOF also require a lot of computational time. In the frame of 2-D vertical flows, other techniques use much less computation time. For irrotationnal flows, solving the Laplace equation can be done very efficiently. The difficulty of this method lies in the definition of boundary conditions. The free-surface, which is naturally determined when using Lagrangian methods, needs a heavy iterative solving due to its non-linear nature when expressed in the frame of the Laplace equation. This paper will present an original technique that allows a quicker and easier determination of the free-surface. An irregular mesh for boundaries is used and discussed. The method is validated with analytical solutions and experimental measurements. [less ▲]Detailed reference viewed: 38 (12 ULg)
How to Statistically Show the Absence of an Effect
in Psychologica Belgica (2011), 51(2), 109-127
In experimental studies, the lack of statistical significance is often interpreted as the absence of an effect. Unfortunately, such a conclusion is often a serious misinterpretation. Indeed, non ... [more ▼]
In experimental studies, the lack of statistical significance is often interpreted as the absence of an effect. Unfortunately, such a conclusion is often a serious misinterpretation. Indeed, non-significant results are just as often the consequence of an insufficient statistical power. In order to conclude beyond reasonable doubt that there is no meaningful effect at the population level, it is necessary to use proper statistical techniques. The present article reviews three different approaches that can be used to show the absence of a meaningful effect, namely the statistical power test, the equivalence test, and the confidence interval approach. These three techniques are presented with easy to understand examples and equations are given for the case of the two-sample t-test, the paired-sample t-test, the linear regression coefficient and the correlation coefficient. Despite the popularity of the power test, we recommend using preferably the equivalence test or the confidence interval. [less ▲]Detailed reference viewed: 43 (9 ULg)
How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing.
; Saegerman, Claude ; et al
in Veterinary Microbiology (2002), 90(1-4), 461-77
Collaborative work was financed by the EU to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to Yersinia enterocolitica O:9 either in ... [more ▼]
Collaborative work was financed by the EU to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to Yersinia enterocolitica O:9 either in conjunction with, or as an alternative to, the classical serological, bacteriological or allergic skin tests. Sixteen heifers were experimentally infected with Brucella abortus biovar 1 (five heifers), Brucella suis biovar 2 (two heifers), Y. enterocolitica O:9 (six heifers) and Y. enterocolitica O:3 (three heifers). Four heifers, naturally infected with Y. enterocolitica O:9 that presented aspecific brucellosis serological reactions were also included in the experiment. A self-limited infection was induced in cattle by B. suis biovar 2. All the brucellosis serological tests used, i.e. the slow agglutination test (SAW), the Rose Bengal test (RB), the complement fixation test (CFT), indirect and competitive ELISA's, lacked specificity when used to analyze sera from Y. enterocolitica O:9 infected animals. A Yersinia outer membrane proteins (YOPs)-ELISA was also used and although the test is able to detect a Yersinia group infection, it provided no evidence of whether or not there is a possible brucellosis infection when dual infections are present. The brucellergen IFN-gamma test showed a lack of specificity also. The only test that was proven to be specific is the brucellergen skin test. All brucellosis serological tests, except the indirect ELISA, were limited in their ability to detect B. abortus persistently infected animals. Based on these experimental studies, a strategy was implemented as part of the year 2001 Belgian Brucellosis Eradication Program to substantiate the eradication of bovine brucellosis. Epidemiological inquiries have identified risk factors associated with aspecific serological reactions, possible transmission and infection of cattle by B. suis biovar 2 from infected wild boars; and both legal and administrative measures taken by the veterinary services. No cases of bovine brucellosis have been confirmed in Belgium since March 2000. [less ▲]Detailed reference viewed: 58 (18 ULg)
How to support the transition of existing urban neighbourhoods?
Conference given outside the academic context (2013)Detailed reference viewed: 7 (2 ULg)
How to survey your hippos night and day? Follow them in bed with drones!
Linchant, Julie ; Lejeune, Philippe ; Vermeulen, Cédric
Scientific conference (2014, October 20)Detailed reference viewed: 44 (5 ULg)