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See detailHistory/Lyric/Speech
Delville, Michel ULg

in Delville, Michel (Ed.) History/Lyric/Speech (2004)

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See detailHistoWeb - Toward a new learning ecology for histology
Vincke, Grégoire ULg; Marée, Raphaël ULg; Wehenkel, Louis ULg et al

Conference (2014, December 16)

HistoWeb targets the transformation of the professional tool Cytomineinto a comprehensive and innovative teaching platform, valuing the notions of learning ecology and new learning dimensions seeking for ... [more ▼]

HistoWeb targets the transformation of the professional tool Cytomineinto a comprehensive and innovative teaching platform, valuing the notions of learning ecology and new learning dimensions seeking for lifelong competencies. The poster was released at the Digital Learning round table, organized by the European Commission around H2020 funding instruments and call 2 "ICT-20 Technologies for better human learning" [less ▲]

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See detailHistoWeb - Transforming a professional bioimages platform into a learning tool for histology
QUATRESOOZ, Pascale ULg; Defaweux, Valérie ULg; Rollus, Loïc ULg et al

Scientific conference (2014, October 30)

In 2012, medical studies in Belgium has undergone an important reform that leads to a rocketing rise of the number of students. The University of Liège took this renewed context as an opportunity to ... [more ▼]

In 2012, medical studies in Belgium has undergone an important reform that leads to a rocketing rise of the number of students. The University of Liège took this renewed context as an opportunity to thoroughly revamp its teaching methods in histology, extending CYTOMINE [1] (http://cytomine.be : a web-based, image storage, annotation, and analysis platform) with new pedagogical features. [less ▲]

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See detailHitchcock, ou les psychoses à l'eau de rose
Tomasovic, Dick ULg

Article for general public (2013)

Consacré à la période de conception de Psychose, le drame biographique "Hitchcock", à l’affiche en 2013, transforme l’une des plus palpitantes aventures cinématographiques en une romance tiède. L’ombre de ... [more ▼]

Consacré à la période de conception de Psychose, le drame biographique "Hitchcock", à l’affiche en 2013, transforme l’une des plus palpitantes aventures cinématographiques en une romance tiède. L’ombre de Psychose est peut-être encore à ce point écrasante que l’on n'ose évoquer ce film légendaire que de loin. [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D1
Johansson, Eva; Fuertes, Nuria; Guvaia, Helena et al

Report (2012)

D1.1: Report on tubular structures of interest subjected to extreme repeated loadings. D1.2: Report on current design procedures for welded/bolted HSS materials, members and connections. D1.3: Report on ... [more ▼]

D1.1: Report on tubular structures of interest subjected to extreme repeated loadings. D1.2: Report on current design procedures for welded/bolted HSS materials, members and connections. D1.3: Report on Bayesian estimations in probabilistic reliability assessment. D1.4: Report on Output-only structural identification techniques. [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D2
Jaspart, Jean-Pierre ULg; Demonceau, Jean-François ULg; Hoang, Van Long ULg et al

Report (2012)

D2.1: Actions on foot- cycle-bridges and railway bridges and stresses on critical elements. D2.2: Validation of MB and FE software and estimation of stresses on critical elements.

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D3
Rivera, Sergio; Alvarez, Ricardo; Lezcano, Ricardo et al

Report (2012)

D3.1: Mechanical, toughness and corrosion properties for HSS employed in this study. D3.2: Cyclic test data of tubular (cylindrical) members or member assemblies D3.3: Buckling test data of tubular ... [more ▼]

D3.1: Mechanical, toughness and corrosion properties for HSS employed in this study. D3.2: Cyclic test data of tubular (cylindrical) members or member assemblies D3.3: Buckling test data of tubular (cylindrical) members or member assemblies [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D4
Rivera, Sergio; Alvarez, Ricardo; Lezcano, Ricardo et al

Report (2012)

D4.1: Materials and fracture-mechanic properties including loading rate and corrosion D4.2: Test data on welded tubular connections D4.3: Performance of advanced post-treatment welding techniques D4.4 ... [more ▼]

D4.1: Materials and fracture-mechanic properties including loading rate and corrosion D4.2: Test data on welded tubular connections D4.3: Performance of advanced post-treatment welding techniques D4.4: Test data on bolted tubular connections [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D5
Jaspart, Jean-Pierre ULg; Demonceau, Jean-François ULg; Hoang, Van Long ULg et al

Report (2012)

D5.1: Refined material parameter formula depending upon fast loading. D5.2: Simulation data on welded joints relevant to the structures under study. D5.3: Simulation data on bolted joints relevant to the ... [more ▼]

D5.1: Refined material parameter formula depending upon fast loading. D5.2: Simulation data on welded joints relevant to the structures under study. D5.3: Simulation data on bolted joints relevant to the structures under study. [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D6
Demofonti, Giuseppe; Zilli, Giuliana; Tamponi, Gian Marco et al

Report (2012)

D6.1: S-N data relevant to connections and refinement of the component method for bolted connections D6.2: Simulations data on tubular members D6.3: Simulation data on case studies and similar structural ... [more ▼]

D6.1: S-N data relevant to connections and refinement of the component method for bolted connections D6.2: Simulations data on tubular members D6.3: Simulation data on case studies and similar structural types. [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D7
Rivera, Sergio; Alvarez, Ricardo; Lezcano, Ricardo et al

Report (2012)

D7.1: Report on design guidelines and recommendations for HSS for tubular structures D7.2: Report on design guidelines and recommendations for tubular members and connections D7.3: Report on maintenance ... [more ▼]

D7.1: Report on design guidelines and recommendations for HSS for tubular structures D7.2: Report on design guidelines and recommendations for tubular members and connections D7.3: Report on maintenance and durability of HSS tubular structures [less ▲]

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See detailHITUBES PROJECT DESIGN AND INTEGRITY ASSESSMENT OF HIGH STRENGTH TUBULAR STRUCTURES FOR EXTREME LOADING CONDITIONS - D8
Bursi, Oreste S.; Kumar, Anil; Demonceau, Jean-François ULg

Report (2012)

D8.1: Evaluation of the results of the SWOT analysis. D8.2: Evaluation of the results of the monitoring activity.

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See detailHIV resistance to antiretroviral drugs: Mechanisms, genotypic and phenotypic resistance testing in clinical practice
Blaise, Pierre ULg; Clevenbergh, P.; Vaira, Dolorès ULg et al

in Acta Clinica Belgica (2002), 57(4, Jul-Aug), 191-201

HIV resistance to antiretroviral agents is a major contributory cause of treatment failure. The dynamics of HIV replication, together with patient-, physician-, and drug-related factors, lead to emergence ... [more ▼]

HIV resistance to antiretroviral agents is a major contributory cause of treatment failure. The dynamics of HIV replication, together with patient-, physician-, and drug-related factors, lead to emergence of HIV resistant strains in most of the patients. Phenotypic assays look for an increase in the antiretroviral drug (ARV) concentration that inhibits 50% of the growth of the tested HIV strain (IC50), comparatively with a reference strain cultivated in parallel. Genotypic tests detect resistance mutations in the reverse transcriptase and protease genes by comparing the gene sequences of a resistant virus to those of a wildtype strain that has previously been described. The efficacy of each ARV class and each individual ARV is threatened by specific mutations and resistance mechanisms. In retrospective studies of genotypic or phenotypic resistance testing, baseline resistance tests results were correlated with virological outcomes. There is some evidence from prospective studies that resistance testing may have some benefits when used to choose salvage regimens. However, problems in the areas of test interpretation, patient compliance, availability of active drugs, and technical test performance limit the usefulness of resistance testing in clinical practice. This article reviews the mechanisms underlying HIV resistance, the principles of phenotypic and genotypic tests, and the use of these tests in clinical practice. [less ▲]

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See detailHIV viraemic patients downregulate CD94/NKG2A inhibitory receptors on NK as well as CD8 T cells in comparison with aviraemic counterparts
Zeddou, Mustapha; Rahmouni, Souad ULg; VANDAMME, Arnaud ULg et al

Poster (2006)

Background: The CD94/NKG2 heterodimer is a C-type lectin receptor formed by the association of CD94 and one of the NKG2 molecules (namely NKG2A, -B, -C or –E). The interaction of CD94/NKG2A with non ... [more ▼]

Background: The CD94/NKG2 heterodimer is a C-type lectin receptor formed by the association of CD94 and one of the NKG2 molecules (namely NKG2A, -B, -C or –E). The interaction of CD94/NKG2A with non classical HLA-E molecules delivers inhibitory signals. CD94/NKG2A is normally expressed on most NK cells whereas less than 5% of peripheral resting CD8+ T cells are positive. Although several reports have clearly shown an upregulation of CD94 on CD8 T cells in HIV infection, the simultaneous expression of both subunits of the inhibitory receptor on NK and T cells and its relation with viral load is largely unknown. Methods: PBLs from 30 HIV-infected patients (16 viraemic and 14 aviraemic under HAART) and 18 healthy volunteers were analysed by flow cytometry after staining with the following monoclonal antibodies (Percp-conjugated anti-CD8, FITC-conjugated anti-CD3, APC-conjugated anti-CD94, PE-conjugated anti-NKG2A). Results: The proportion of CD8 T cells expressing the CD94/NKG2A inhibitory receptor was not significantly increased in HIV-infected patients (5.68 ± 3.72%) in comparison with non-infected controls (4.90±2.84%). Interestingly, patients with viral load < 50 copies/ml had a higher proportion of CD8 T cells expressing the inhibitory receptor (7.15 ± 3.63%) than patients with HIV viraemia (4.40 ± 3.40%), p= 0.041. The same pattern was observed for NK cells and was even more pronounced. In aviraemic individuals, 61.75 ± 20.39% of NK cells expressed the inhibitory receptor vs 42.88 ± 26.38% in viraemic patients. The proportion of CD94/NKG2A positive cells was correlated between NK and CD8 T cell subsets (p=0.0351) but there was no correlation with absolute or relative CD4 counts. Conclusions: Our results suggest that chronic stimulation with HIV antigens in viraemic patients could lead to decreased rather than increased expression of inhibitory receptors on NK and CD8 T cells. This could contribute to the abnormal activation of the immune system associated with advanced HIV disease. [less ▲]

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See detailHIV-1 and NF-kappaB activation by photo-oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie ULg; Piret, Bernard

in Photochemistry & Photobiology (1995)

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See detailHIV-1 evades innate immune recognition through specific cofactor recruitment
Rasaiyaah, Jane; Ping Tan, Choon; Fletcher, Adam et al

in Nature (2013)

Human immunodeficiency virus (HIV)-1 is able to replicate in primary human macrophages without stimulating innate immunity despite reverse transcription of genomic RNA into double-stranded DNA, an ... [more ▼]

Human immunodeficiency virus (HIV)-1 is able to replicate in primary human macrophages without stimulating innate immunity despite reverse transcription of genomic RNA into double-stranded DNA, an activity that might be expected to trigger innate pattern recognition receptors. We reasoned that if correctly orchestrated HIV-1 uncoating and nuclear entry is important for evasion of innate sensors then manipulation of specific interactions between HIV-1 capsid and host factors that putatively regulate these processes should trigger pattern recognition receptors and stimulate type 1 interferon (IFN) secretion. Here we show that HIV-1 capsid mutants N74D and P90A, which are impaired for interaction with cofactors cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and cyclophilins (Nup358 and CypA), respectively, cannot replicate in primary human monocyte-derived macrophages because they trigger innate sensors leading to nuclear translocation of NF-κB and IRF3, the production of soluble type 1 IFN and induction of an antiviral state. Depletion of CPSF6 with short hairpin RNA expression allows wild-type virus to trigger innate sensors and IFN production. In each case, suppressed replication is rescued by IFN-receptor blockade, demonstrating a role for IFN in restriction. IFN production is dependent on viral reverse transcription but not integration, indicating that a viral reverse transcription product comprises the HIV-1 pathogen-associated molecular pattern. Finally, we show that we can pharmacologically induce wild-type HIV-1 infection to stimulate IFN secretion and an antiviral state using a non-immunosuppressive cyclosporine analogue. We conclude that HIV-1 has evolved to use CPSF6 and cyclophilins to cloak its replication, allowing evasion of innate immune sensors and induction of a cell-autonomous innate immune response in primary human macrophages. [less ▲]

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See detailHiv-1 Gp41 And Gp160 Are Hyperthermostable Proteins In A Mesophilic Environment - Characterization Of Gp41 Mutants
Krell, Tino; Greco, Frédéric; Engel, Olivier et al

in European Journal of Biochemistry (2004), 271(8), 1566-79

HIV gp41(24-157) unfolds cooperatively over the pH range of 1.0-4.0 with T(m) values of > 100 degrees C. At pH 2.8, protein unfolding was 80% reversible and the DeltaH(vH)/DeltaH(cal) ratio of 3.7 is ... [more ▼]

HIV gp41(24-157) unfolds cooperatively over the pH range of 1.0-4.0 with T(m) values of > 100 degrees C. At pH 2.8, protein unfolding was 80% reversible and the DeltaH(vH)/DeltaH(cal) ratio of 3.7 is indicative of gp41 being trimeric. No evidence for a monomer-trimer equilibrium in the concentration range of 0.3-36 micro m was obtained by DSC and tryptophan fluorescence. Glycosylation of gp41 was found to have only a marginal impact on the thermal stability. Reduction of the disulfide bond or mutation of both cysteine residues had only a marginal impact on protein stability. There was no cooperative unfolding event in the DSC thermogram of gp160 in NaCl/P(i), pH 7.4, over a temperature range of 8-129 degrees C. When the pH was lowered to 5.5-3.4, a single unfolding event at around 120 degrees C was noted, and three unfolding events at 93.3, 106.4 and 111.8 degrees C were observed at pH 2.8. Differences between gp41 and gp160, and hyperthermostable proteins from thermophile organisms are discussed. A series of gp41 mutants containing single, double, triple or quadruple point mutations were analysed by DSC and CD. The impact of mutations on the protein structure, in the context of generating a gp41 based vaccine antigen that resembles a fusion intermediate state, is discussed. A gp41 mutant, in which three hydrophobic amino acids in the gp41 loop were replaced with charged residues, showed an increased solubility at neutral pH. [less ▲]

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See detailHIV-1 promoter activation following an oxidative stress mediated by singlet oxygen
Legrand, Sylvie ULg; Hoebeke, Maryse ULg; Vaira, Dolorès ULg et al

in Journal of Photochemistry and Photobiology B : Biology (1993), 17(3), 229-237

Various biological processes, such as photosensitization or inflammatory reactions, can generate singlet oxygen (O-1(2)) as one of the major oxidative species. Because this oxidant can be generated either ... [more ▼]

Various biological processes, such as photosensitization or inflammatory reactions, can generate singlet oxygen (O-1(2)) as one of the major oxidative species. Because this oxidant can be generated either extracellularly or intracellularly, it can cause severe damage to various biological macromolecules, even to those deeply embedded inside the cells such as DNA. Sublethal biological modifications induced by different DNA-damaging agents can promote various cellular responses initiated by the activation of various cellular genes and certain heterologous viruses. Since O-1(2) fulfils essential prerequisites for a genotoxic substance, we have examined the effects of an oxidative stress, mediated by this species, on cells harbouring a heterologous promoter-leader sequence derived from the human immunodeficiency virus type 1 (HIV-1). Our results demonstrate that HIV-1 long terminal repeat (LTR), integrated into the cellular I)NA of epithelial cells, can be transactivated following an oxidative stress mediated by O-1(2). In addition, using HIV-1 latently infected promonocytes or lymphocytes, it can be shown that virus reactivation can be induced through a sublethal dose of O-1(2) generated intracellularly. An extracellular generation of O-1(2) can promote a substantial lethal effect without HIV-1 reactivation. These data may be relevant to the understanding of the events converting a latent infection into a productive one and to the appearance of the acquired immune deficiency syndrome. [less ▲]

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