Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailThe incorporation of a non-natural amino acid (aza-tryptophan) may help to crystallize a protein and to solve its crystal structure. Application to bacteriophage lambda lysozyme.
Evrard, Christine ULg; Fastrez, Jacques; Declercq, Jean-Paul

in Acta Crystallographica Section D-Biological Crystallography (1999), D55

Until now, wild-type bacteriophage lambda lysozyme had been impossible to crystallize. This difficulty could be overcome by the replacement of the four tryptophan residues by azatryptophans. Analysis of ... [more ▼]

Until now, wild-type bacteriophage lambda lysozyme had been impossible to crystallize. This difficulty could be overcome by the replacement of the four tryptophan residues by azatryptophans. Analysis of the intermolecular and intramolecular contacts in this modification allows understanding of the differences in behaviour between the native and modified molecules. Furthermore, this mutation was very useful for the creation of new heavy-atom binding sites and for the solution of the non-crystallographic symmetry, which is extremely important for phase improvement. This procedure seems to be generally applicable, at least in the search for new possibilities for heavy-atom binding sites. [less ▲]

Detailed reference viewed: 13 (0 ULg)
See detailIncorporation of prior information in the regularized inversion of ERT data with CRTomo
Hermans, Thomas ULg; Caterina, David ULg; Martin, Roland et al

Conference (2012, February 03)

The electrical resistivity tomography (ERT) inverse problem is ill-posed leading to non-uniqueness of its solution. A possible way to reduce the amount of mathematical solutions to more geologically ... [more ▼]

The electrical resistivity tomography (ERT) inverse problem is ill-posed leading to non-uniqueness of its solution. A possible way to reduce the amount of mathematical solutions to more geologically plausible models is to incorporate priori information in the inversion process. In this presentation, we describe the implementation of three different techniques ways to go beyond standard Occam’s inversion for ERT with the code CRTomo : a simple reference model weighted by a user-defined parameter, a structural constraint defined by modifying the construction of the roughness matrix compared to standard Occam’s inversion and a geostatistical constraint based on a vertical correlation length deduced from borehole data to build the model covariance matrix and use it to regularize the inversion. With several examples, we show that the traditional smoothness constraint yield small contrasts of resistivity, far from the reality revealed by borehole measurements. Incorporating prior information from boreholes clearly or from another geophysical technique (like GPR or seismic refraction) improves the misfit with logging data. The choice of the prior information incorporation technique highly depends on which king of information is available and further developments are needed to extend the techniques to more complex cases. [less ▲]

Detailed reference viewed: 60 (13 ULg)
Full Text
Peer Reviewed
See detailIncorporation of rapeseed meal extracted by pressure in a diet for growing fattening bulls .1. Intake, digestibility and fermentation in the rumen
Mayombo, A. P.; Baldwin, Paule ULg; Wathelet, Jean-Paul ULg et al

in Annales de Zootechnie (1997), 46(1), 57-70

Intake, digestibility, degradation kinetic and fermentation were studied with bulls offered fattening diets with four different incorporation rates of rapeseed meal obtained by pressure; the ether extract ... [more ▼]

Intake, digestibility, degradation kinetic and fermentation were studied with bulls offered fattening diets with four different incorporation rates of rapeseed meal obtained by pressure; the ether extract content was 8.6% and the glucosinolate content 17,6 mu molg. The control diet was made of sugar beet pulp, cereals, soya bean meal and linseed meal. In the three other diets, nitrogen from rapeseed meal provided 33, 66 or 100% of nitrogen from soya bean meal or linseed meal. The intakes of the control diet were 94.3 +/- 7.3 g/kg metabolic body weight. Intakes were maintained high in the diet with 33, 66 or 100% of protein from rapeseed meal at 93.5 +/- 7.7; 93.0 +/- 5.0 and 93.5 +/- 7.7 g/kg, respectively. The apparent digestibility of rapeseed meal in the ration containing only rapeseed meal was high at 74.9 +/- 1.9% for dry matter, 76.5 +/- 2.1% for organic matter, 69.9 +/- 3.9% for nitrogen, 59.1 +/- 4.8% for ADF and 74.6 +/- 6.9% for ether extract. Dry matter of rapeseed meal was less degradable (57.8 +/- 5.0%) than that of soya bean meal (65.5 +/- 2.6%) or of linseed meal (61.6 +/- 4.2%) but was not as well protected than that of protected soya bean meal (43.6 +/- 2.6%) (P < 0.001). Similar trends were observed for the degradability of nitrogen (P < 0.001). Ether extract of rapeseed meal was more degradable than that of linseed meal (P < 0.001). There were no significant effects of the incorporation rate of rapeseed meal on the pH in the rumen. The fermentations were mainly of the acetate type with usual concentrations of propionate, butyrate, total volatile fatty acids, glucose or alpha amino nitrogen. Ammonia concentration was higher with the control diet and lower with the diet with 100% of protein from rapeseed meal. Fatty acids of rapeseed meal were more protected against saturation by the rumen microorganisms than in linseed meal. [less ▲]

Detailed reference viewed: 40 (6 ULg)
Full Text
Peer Reviewed
See detailIncorporation of rapeseed meal extracted by pressure in a diet for growing fattening bulls .2. Glucosinolates and their breakdown products in the rumen
Wathelet, Jean-Paul ULg; Istasse, Louis ULg; Mayombo, A. P. et al

in Annales de Zootechnie (1997), 46(1), 71-79

Eight bulls from the Belgian Blue breed were offered diets in which soya bean meal and linseed meal were substituted by rapeseed meal (variety Samourai) in order to provide, respectively, 33, 66 and 100 ... [more ▼]

Eight bulls from the Belgian Blue breed were offered diets in which soya bean meal and linseed meal were substituted by rapeseed meal (variety Samourai) in order to provide, respectively, 33, 66 and 100% of the protein. The glucosinolate content was equal to 17.6 mu mol/g dry matter. Analyses of wet residues from rapeseed meal incubated in the rumen showed a rapid decrease of the glucosinolate content which disappeared after 5 h of incubation. The 3-hydroxypent-4-enenitrile, the but-3-enylisothiocyanate and the 5-vinyl-1,3-oxazolidine-2-thione antinutritional factors obtained from the glucosinolates were identified by GC-MS during the first 5 h. The 3-phenylpropanenitrile, the 3-methylindol, the 2-phenylacetic and 3-phenylpropanoic acids were also present in the residues and in the rumen. Although high sensitivity of the methods were used, no glucosinolates and their direct breakdown products were detected in the liquid and the solid content of the rumen. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Peer Reviewed
See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULg; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, October 27)

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULg; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, September 05)

Detailed reference viewed: 11 (1 ULg)
Peer Reviewed
See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULg; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, September 04)

Detailed reference viewed: 6 (1 ULg)
Peer Reviewed
See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULg; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, June 10)

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailIncrease in Cytokine Production (Il-1 Beta, Il-6, Tnf-Alpha but Not Ifn-Gamma, Gm-Csf or Lif) by Stimulated Whole Blood Cells in Postmenopausal Osteoporosis
Zheng, S. X.; Vrindts-Gevaert, Yvonne; Lopez, Marie-Josée ULg et al

in Maturitas (1997), 26(1), 63-71

Postmenopausal osteoporosis is a progressive disorder characterized by a decreased bone mass and increased susceptibility to fractures. Several investigations have suggested that one of the mechanisms ... [more ▼]

Postmenopausal osteoporosis is a progressive disorder characterized by a decreased bone mass and increased susceptibility to fractures. Several investigations have suggested that one of the mechanisms through which estrogen prevents bone loss was a modulation on secretion or release of various cytokines that are known to influence bone remodeling, even if some recent data have challenged this hypothesis. However, in established osteoporosis, the possibility that enhanced cytokines activity may account for the progression of this disease remains unclear and controversial. We sought here to determine whether production of IL-1 beta, IL-6, TNF-alpha, IFN-gamma, GM-CSF and LIF, after direct stimulation in whole blood, was different in healthy (n = 30) or osteoporotic postmenopausal women (n = 24) and whether lumbar bone density (1-BMD) correlated with the values of cytokine production observed in these conditions. A significant difference was observed between the osteoporotic and control subjects for IL-1 beta (p < 0.0001), IL-6 (p < 0.001) and TNF-alpha (p = 0.027) productions, the values being higher in the osteoporotic women. No significant differences between the groups were observed for IFN-gamma (p = 0.51), GM-CSF (p = 0.70) or LIF (p = 0.97). In the whole population, statistically significant negative correlations were observed between lumbar BMD and IL-1 beta (r = -0.46) (p < 0.0005), IL-6 (r = -0.50) (p < 0.0001) and TNF-alpha (r = -0.39) (p < 0.005) production while no such correlations were observed for IFN-gamma, GM-CSF or LIF. In conclusion, the study of cytokine production by immune cells cultured in autologous whole blood suggests that in women more than 10 years past the menopause and presenting a decrease in lumbar bone density corresponding to the new WHO definition of "osteoporosis', production of IL-1 beta, IL-6 and TNF-alpha is still increased compared to controls matched for age and ovarian function, while no differences are reported for IFN-gamma, GM-CSF or LIF production. [less ▲]

Detailed reference viewed: 56 (6 ULg)
Full Text
Peer Reviewed
See detailIncrease in dimethylsulfide (DMS) emissions due to eutrophication of coastal waters offsets their reduction due to ocean acidification
Gypens, N; Borges, Alberto ULg

in Frontiers in Marine Science - Marine Ecosystem Ecology (2014), 1(4),

Available information from manipulative experiments suggested that the emission of dimethylsulfide (DMS) would decrease in response to the accumulation of anthropogenic CO2 in the ocean (ocean ... [more ▼]

Available information from manipulative experiments suggested that the emission of dimethylsulfide (DMS) would decrease in response to the accumulation of anthropogenic CO2 in the ocean (ocean acidification). However, in coastal environments, the carbonate chemistry of surface waters was also strongly modified by eutrophication and related changes in biological activity (increased primary production and change in phytoplankton dominance) during the last 50 years. Here, we tested the hypothesis that DMS emissions in marine coastal environments also strongly responded to eutrophication in addition to ocean acidification at decadal timescales. We used the R-MIRO-BIOGAS model in the eutrophied Southern Bight of the North Sea characterized by intense blooms of Phaeocystis that are high producers of dimethylsulfoniopropionate (DMSP), the precursor of DMS. We showed that, for the period from 1951 to 2007, eutrophication actually led to an increase of DMS emissions much stronger than the response of DMS emissions to ocean acidification. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailIncrease in femoral neck bone mineral density is associated with decrease in hip fracture incidence during treatment with strontium ranelate
Bruyère, Olivier ULg; Delmas, Pierre D; Devogelaer, Jean-Pierre et al

in Arthritis and Rheumatism (2006, November), 54(Suppl), 586

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailIncrease in IL-1bêta, IL-6, and TNF-alpha production in postmenopausal osteoporosis
Zheng, SX; Geenen, Vincent ULg; Vrindts, Y et al

in Osteoporosis International (1996), 6(S1), 101

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailINCREASE IN LEVELS OF STRATOSPHERIC CHLORINE AND FLUORINE LOADING BETWEEN 1985 AND 1992
Gunson, M. R.; Abrams, M. C.; Lowes, L. L. et al

in Geophysical Research Letters (1994), 21(20), 2223-2226

Mixing ratios of 3.44 ppbv (parts per billion by volume) and 1.23 ppbv for HCl and HF above 50 km, surrogates for total chlorine and fluorine, have been measured by the Atmospheric Trace Molecule ... [more ▼]

Mixing ratios of 3.44 ppbv (parts per billion by volume) and 1.23 ppbv for HCl and HF above 50 km, surrogates for total chlorine and fluorine, have been measured by the Atmospheric Trace Molecule Spectroscopy (ATMOS) experiment on a March 1992 flight of the Space Shuttle. Compared to the measured values obtained on a 1985 flight, these correspond to a 37% and 62% increase for HCl and HF, respectively. The derived trend in HCl (similar to 0.13 ppbv per year) is in good agreement with the model-predicted increase in chlorine loading of 0.13 ppbv per year [Prather and Watson. 1990], and with the measured trends in HCl total column abundance from reported ground-based observations. The main source of this change can be attributed to the release of man-made chlorofluorocarbons (CFCs) and hydrochlorofluorocarbons (HCFCs). This new value for HCl represents an upper limit to the inorganic chlorine concentration in the stratosphere available for participation in photochemical processes which destroy ozone. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
See detailIncrease in Soil Macroporosity managed with Winter Ploughing - a preliminary results
Parvin, Nargish ULg; Chelin, Marie ULg; Hiel, Marie-Pierre ULg et al

Poster (2014, February 07)

Measurement of soil water retention capacity by the conventional pressure plate technique always gives a substantial view of soil porosity distribution. The structural orientation is observed in the ... [more ▼]

Measurement of soil water retention capacity by the conventional pressure plate technique always gives a substantial view of soil porosity distribution. The structural orientation is observed in the beginning (higher water retention at 9.8 to 98 hPa water head pressure indicates greater proportion of macroporosity) of the soil moisture characteristic curve obtained from the water retention measurement. Since, tillage practices generally increase soil porosity, the correlation between soil hydraulics and porosity distribution would expect to be different for different tillage systems. In general, macroporosity increase with the adoption of conservative tillage or no tillage system but the changes can be varied with the seasonal variation. In our study, winter ploughing retains more water at the range of 9.8 to 98 hPa than Strip tillage, No-till residues in and No-till residues out. So, we can conclude that there is certainly increase in macroporosity in ploughing than other conservation practices of reduced tillage and no tillage. [less ▲]

Detailed reference viewed: 35 (11 ULg)
See detailIncrease in the extracellular lipase Production by Yarrowia lipolytica.
Destain, Jacqueline ULg; Roblain, D.; Thonart, Philippe ULg

Poster (1994, July)

Detailed reference viewed: 3 (0 ULg)
Peer Reviewed
See detailIncrease in the number and the phagocytic function of guinea pig pulmonary and peritoneal macrophages following oral administration of RU 41740, a glycoprotein extract from Klebsiella pneumoniae.
Radermecker, Maurice ULg; Rommain, M.; Maldague, M. P. et al

in International journal of immunopharmacology (1988), 10(8), 913-7

RU 41740 (Biostim) which is a purified glycoprotein extract from Klebsiella pneumoniae, is an orally active non-specific immunostimulant. In guinea pigs, 8 days after a 7 days oral administration of RU ... [more ▼]

RU 41740 (Biostim) which is a purified glycoprotein extract from Klebsiella pneumoniae, is an orally active non-specific immunostimulant. In guinea pigs, 8 days after a 7 days oral administration of RU 41740 (10 or 100 mg/kg/day), an increase in the cell population of the pulmonary and peritoneal cavities was observed, especially in that of the macrophages. RU 41740 also enhanced the phagocytic activity of both the alveolar and peritoneal macrophages, when their chemotactic activity was not significantly modified. This increase in the number of pulmonary macrophages and the stimulation of their phagocytic function might explain the protective effect afforded by the oral administration of Biostim against respiratory infections in patients with chronic bronchitis. [less ▲]

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailIncrease in type II collagen turnover after iron depletion in patients with hereditary haemochromatosis.
Richette, Pascal; Eymard, Claire; Deberg, Michelle ULg et al

in Rheumatology (2010), 49(4), 760-6

OBJECTIVE: To determine the effects of iron depletion on serum levels of joint biomarkers and on joint symptoms in patients with hereditary haemochromatosis (HH). METHODS: Levels of biomarkers were ... [more ▼]

OBJECTIVE: To determine the effects of iron depletion on serum levels of joint biomarkers and on joint symptoms in patients with hereditary haemochromatosis (HH). METHODS: Levels of biomarkers were measured in 18 patients with HH at the time of diagnosis and after iron depletion. The markers were type II collagen degradation (Coll2-1) and its nitrated form (Coll2-1NO(2)), type II procollagen synthesis (CPII), MPO, COMP and HA. For each patient, demographic data were collected and the global joint pain (visual analogue scale) was assessed before and after iron depletion by phlebotomy. RESULTS: A total of 18 patients [10 males; mean (s.d.) age 48 (11) years] were homozygous for the C282Y mutation. No patient had liver dysfunction. Ferritin level before iron removal was 627.5 (range 133-3276) microg/l, and duration of the iron depletion phase was 295 (70-670) days. Serum levels of both Coll2-1 and CPII were significantly increased from diagnosis after iron depletion: 80.1 (55.6-113.5) vs 96.0 (48.8-136.3) nM (P = 0.004) and 731.4 (374.2-1012.3) vs 812.8 (535.8-1165.6) ng/ml (P = 0.03), respectively. Levels of other biomarkers were not modified by iron depletion. Ferritin level, which at baseline was correlated with body iron store (r = 0.63; P = 0.008), was significantly correlated with HA level measured before iron depletion (r = 0.60; P = 0.01). Global joint pain was not correlated with ferritin concentration and did not significantly decrease after iron depletion: 43 (19-73) vs 36 (16-67) mm (P = 0.07). CONCLUSIONS: In patients with HH, cartilage homoeostasis is modified by iron excess and an increase in type II collagen turnover occurs after excess iron removal. [less ▲]

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailIncrease in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.
Theelen, Wendy; Speel, Ernst*-Jan M; Herfs, Michael ULg et al

in American Journal of Pathology (2010), 177(4), 2022-33

Oncogenic human papillomavirus (HPV) infection is the most important risk factor in cervical carcinogenesis cases; high viral loads, viral integration into the host genome, and gain of the telomerase ... [more ▼]

Oncogenic human papillomavirus (HPV) infection is the most important risk factor in cervical carcinogenesis cases; high viral loads, viral integration into the host genome, and gain of the telomerase-related genes, TERT and TERC, are all factors associated with progression to cancer. A recently developed multiparameter HPV 16/18 multiplex ligation-dependent probe amplification (MLPA) assay, which allows the simultaneous assessment of these factors, was applied to a series of 67 normal and (pre)malignant frozen uterine cervical samples, as well as to 91 cytological preparations, to test the ability of the MLPA assay to identify high-risk lesions on the basis of these factors. Validation was performed using quantitative PCR, the PapilloCheck and fluorescence in situ hybridization. Only 5 out of 37 normal tissue samples or low-grade cervical lesions (ie, CIN1 and condyloma) showed either an HPV16 viral load higher than 25 copies per cell, viral integration, and/or gain of one of the telomerase-related genes, whereas for the high-grade cervical lesions, one or more of these risk factors was found in 25 of 30 cases. The HPV MLPA assay showed a sensitivity of 83% and a specificity of 86% in frozen cervical specimens. Furthermore, the feasibility of the MLPA assay was shown for cytological samples, where in 57% of high-grade squamous intraepithelial lesion cases, the high-risk factors were detected using this assay. [less ▲]

Detailed reference viewed: 17 (3 ULg)