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See detailImmunohistochemical detection of incipient melanoma micrometastases. Relationship with sentinel lymph node involvement.
Claessens, Nadine; Pierard, Gérald ULg; Pierard, Claudine ULg et al

in Melanoma Research (2005), 15(2), 107-10

It is acknowledged that tumour thickness, ulceration and lymph node invasion are the most important prognostic factors for cutaneous melanomas. Other histopathological features may also be informative ... [more ▼]

It is acknowledged that tumour thickness, ulceration and lymph node invasion are the most important prognostic factors for cutaneous melanomas. Other histopathological features may also be informative. The aim of this study was to ascertain whether immunohistochemical methods can improve the detection of satellite micrometastases in primary melanoma patients. In addition, the predictive value of cutaneous satellite micrometastases for sentinel lymph node involvement was evaluated. A total of 265 primary cutaneous melanomas and 68 of the respective sentinel nodes were studied using a panel of seven antibodies directed against melanocyte-related antigens. In 12.4% of the 265 cases, small satellite micrometastases were detected by immunohistochemistry. Sentinel lymph node metastases were found in 14% of the 68 cases. Invasion of the sentinel lymph node correlated with the presence of cutaneous satellite micrometastases. It is concluded that the presence of cutaneous satellite micrometastases may be an indication for the performance of sentinel lymph node biopsy, and this finding calls for a closer follow-up of these patients. [less ▲]

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See detailImmunohistochemical evidence for cholecystokinin-like peptides in neuronal cell bodies of the rat spinal cord.
Vanderhaeghen, J. J.; Deschepper, C.; Lotstra, F. et al

in Cell & Tissue Research (1982), 223(2), 463-7

Cholecystokinin-like immunoreactivity has been demonstrated by radioimmunoassay and immunocytochemistry in the spinal cord of various mammals, in particular in nerve fibers of the superficial layers of ... [more ▼]

Cholecystokinin-like immunoreactivity has been demonstrated by radioimmunoassay and immunocytochemistry in the spinal cord of various mammals, in particular in nerve fibers of the superficial layers of the posterior column, but had not been detected in neuronal cell bodies. We report immunohistochemical evidence for the presence of a group of cholecystokinin-containing neuronal cell bodies in the lumbar spinal cord of the rat. This group of cells is only visualized after direct injection of colchicine into the spinal cord and is located near the central canal in the intermedio-medial nucleus of area X of Rexed. [less ▲]

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See detailImmunohistochemical expression of epidermal growth factor receptors in nuclei of a subpopulation of keratinocytes and sweat gland cells.
Pierard, Claudine ULg; Colige, Alain ULg; Arrese Estrada, Jorge ULg et al

in Dermatologica (1991), 183(1), 7-9

We have raised a polyclonal antibody to the 170-kD epidermal growth factor receptor. We found an intercellular pattern of immunoreactivity in the epidermis as well as a positivity of the cytoplasm of ... [more ▼]

We have raised a polyclonal antibody to the 170-kD epidermal growth factor receptor. We found an intercellular pattern of immunoreactivity in the epidermis as well as a positivity of the cytoplasm of keratinocytes and eccrine secretory cells. In some samples, a nuclear labelling was evidenced in these type of cells. There is a close resemblance in the topographical distribution of these cells with nuclear labelling and those synthesizing DNA under phytohaemagglutinin stimulation. [less ▲]

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See detailImmunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use.
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in Journal of Medical Virology (1995), 47(4), 342-7

Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical ... [more ▼]

Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical and immunohistochemical identification of the VZV ORF63 encoded protein (IE63) and of the VZV late protein gE on smears and formalin-fixed paraffin-embedded skin sections taken from lesions clinically diagnosed as varicella (n = 15) and herpes zoster (n = 51). Microscopic examinations of Tzanck smears and skin sections yielded a diagnostic accuracy of Herpesviridae infections in 66.7% (10/15) and 92.3% (12/13) of varicella, and 74.4% (29/39) and 87.8% (43/49) of herpes zoster, respectively. Immunohistochemistry applied to varicella provided a type-specific virus diagnostic accuracy of 86.7% (13/15; IE63) and 100% (15/15; gE) on smears, and of 92.3% for both VZV proteins on skin sections. In herpes zoster, the diagnostic accuracy of immunohistochemistry reached 92.3% (36/39; IE63) and 94.9% (37/39; gE) on smears, and 91.7% (44/48; IE63) and 91.8% (45/49; gE) on skin sections. These findings indicate that the immunohistochemical detection of IE63 and gE on both smears and skin sections yields a higher specificity and sensitivity than standard microscopic assessments. [less ▲]

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See detailImmunohistochemical investigation of alpha1 (IV) and alpha5 (IV) collagen chains in a broad spectrum of melanocytic tumours.
Quatresooz, Pascale ULg; Pierard, Gérald ULg

in Melanoma Research (2005), 15(3), 161-8

BACKGROUND: Cells of melanocytic naevi and cutaneous malignant melanomas (MM) are surrounded by a basement membrane (BM). AIM: To scrutinize any difference between the deposits of alpha1 (IV) and alpha5 ... [more ▼]

BACKGROUND: Cells of melanocytic naevi and cutaneous malignant melanomas (MM) are surrounded by a basement membrane (BM). AIM: To scrutinize any difference between the deposits of alpha1 (IV) and alpha5 (IV) collagen chains in melanocytic naevi and MM. METHODS: A total of 27 common melanocytic naevi, 11 dysplastic naevi, 21 atypical naevi (melanocytomas) including Spitz and non-Spitz types, as well as 24 MM were studied. Their phenotypic and functional characteristics defined by immunohistochemistry using a panel of antibodies, including those directed to the alpha1 (IV), alpha3 (IV) and alpha5 (IV) collagen chains. RESULTS: Almost all naevi and half the melanocytomas exhibited a strong positivity for the alpha1 (IV) collagen chain. By contrast, the remaining melanocytomas and MM presented a heterogeneous staining pattern for the alpha1 (IV) collagen chain. One third of the naevi, 23% of the MM without cutaneous micrometastasis and 83% of MM with cutaneous micrometastasis showed discrete cytoplasmic positivity for the alpha5 (IV) collagen chain. All other melanocytic tumours were negative for this antibody. Rare MM cells in transepidermal migration were stained with the anti-alpha1 (IV) or alpha5 (IV) collagen chain antibodies. No immunoreactivity for the alpha3 (IV) collagen chain was disclosed in any of the samples. CONCLUSION: We report the expression of alpha1 (IV) and alpha5 (IV) collagen chains in naevi and MM. The inconsistent staining pattern for alpha1 (IV) collagen chain in phenotypically atypical melanocytomas and in MM highlight the heterogeneity in both cell differentiation and stroma-tumour interactions. This biological aspect may be related to neoplastic progression and influence metastatic potential. [less ▲]

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See detailImmunohistochemical investigation of cells expressing CD21, membrane IgM, CD32, and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves
Chattha, K. S.; Hodgins, D. C.; DeLay, J. et al

in Veterinary Immunology and Immunopathology (2010), 137

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See detailImmunohistochemical localization of basement membrane components and interstitial collagen types in preovulatory rat ovarian follicles.
Palotie, A.; Peltonen, L.; Foidart, Jean-Michel ULg et al

in Collagen and Related Research (1984), 4(4), 279-87

Cryosections of ovaries from rats treated with pregnant mare serum gonadotropin (PMSG) were immunostained with antibodies against collagen types, I, III, IV and V, laminin and heparan sulphate ... [more ▼]

Cryosections of ovaries from rats treated with pregnant mare serum gonadotropin (PMSG) were immunostained with antibodies against collagen types, I, III, IV and V, laminin and heparan sulphate proteoglycan using the peroxidase-antiperoxidase technique. A uniform belt-like staining was observed between the granulosa and theca interna layers with antibodies against type IV collagen, laminin and heparan sulphate proteoglycan. Interstitial collagens type I and III stained the connective tissue in the theca externa layer but gave only faint staining in the area between the granulosa and theca interna zones. If the PMSG-treated rats were injected with human chorionic gonadotropin the number of follicles that showed a discontinuous, ragged or disrupted staining reaction in the area between the granulosa and theca interna layers was clearly increased. Surprisingly in such cases also a punctate staining reaction was seen in the basal granulosa cells. When the rats were injected with radioactive human chorionic gonadotropin, most of the ovarian follicles were labeled within 2 hours. These follicles still showed a continuous staining reaction with antibodies against basement membrane components at the border of the granulosa and theca interna cell layers. These results suggest that there is a basement membrane-like structure in Graafian follicles between the granulosa cells and theca interna layer, and that this basement membrane is disrupted after human chorionic gonadotropin stimulation. [less ▲]

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See detailImmunohistochemical localization of laminin, type IV and type V collagen in basal cell carcinoma.
Van Cauwenberge, D.; Pierard, Gérald ULg; Foidart, Jean-Michel ULg et al

in British Journal of Dermatology (1983), 108(2), 163-70

Laminin, type IV and type V collagen were localized in twenty-five basal cell carcinomas (BCCs) by immunofluorescence. All basal cell carcinomas were separated from the connective tissue by a continuous ... [more ▼]

Laminin, type IV and type V collagen were localized in twenty-five basal cell carcinomas (BCCs) by immunofluorescence. All basal cell carcinomas were separated from the connective tissue by a continuous basement membrane (BM) which always contained the three antigenic determinants. Nodular and cystic BCCs, but not pagetoid BCCs, contain linear and globular deposits of these BM components. The linear deposits probably represent BM debris that remains undigested and the globular deposits correspond to residual cytoplasmic fragments occurring during apoptosis. These results suggest that BM components are synthesized by epithelial cells and are secreted to polymerize at the dermo-epidermal junction. [less ▲]

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See detailImmunohistochemical patterns in the interfollicular Caucasian scalps. Influence of age-, gender- and alopecia.
Franchimont, Claudine ULg; Loussouarn, Genevière; Panhard, Ségolène et al

in BioMed Research International (2013), 2013(769489),

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See detailAn immunohistochemical study of canine nasal aspergillosis
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 132(4), 283-288

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most ... [more ▼]

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most consistent histopathological finding was a severe, predominantly lymphoplasmacytic, inflammatory infiltration of the lamina propria. Fungal hyphae were not observed to invade the mucosa but were found at the mucosal surface and within material collected from the nasal cavity. The main immunohistochemical findings were (1) a predominance of IgG(+) plasma cells over IgA(+) and IgM(+) plasma cells, (2) significant numbers of macrophages and dendritic cells expressing MHC class 11 molecules, (3) macrophages and neutrophils expressing L1 antigen and (4) a mixture of CD4(+) and CD8(+) T cells. These findings are consistent with a dominant Th1-regulated cell-mediated immune response. The nature of the inflammatory infiltrate and the lack of invasiveness of the mucosa by the fungus, together with the clinical course of the disease and the apparent immunocompetence of the affected dogs, suggest that canine nasal aspergillosis resembles the chronic erosive non-invasive fungal sinusitis described in human patients. © 2004 Elsevier Ltd. All rights reserved. [less ▲]

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See detailAn immunohistochemical study of canine nasal aspergillosis.
Peeters, Dominique ULg; day, M. J.; Moore, Peter et al

in Proceedings of The 14th Annual Congress of the ECVIM-CA (2004)

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See detailAn immunohistochemical study of eosinophilic bronchopneumopathy in dogs.
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Proceedings of the 14th Annual Congress of the ECVIM-CA (2004)

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See detailAn Immunohistochemical Study of the Inflammatory Infiltrate Associated with Nasal Carcinoma in Dogs and Cats.
Vanherberghen, Morgane ULg; Day, M. J.; Gabriel, Alexandra et al

in Journal of Comparative Pathology (2009), 141(1)

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See detailImmunohistochemical study of the proliferation index, oestrogen receptors and progesterone receptors A and B in leiomyomata and normal myometrium during the menstrual cycle and under gonadotrophin-releasing hormone agonist therapy
NISOLLE, Michelle ULg; Gillerot, Stéphane; Casanas-Roux, Françoise et al

in Human Reproduction (1999), 14(11), 2844-2850

The cell proliferation-associated antigen Ki 67 and the immunohistochemical content of oestrogen receptors (ER), progesterone receptors AB (PRAB) and progesterone receptors B (PRB) were evaluated in ... [more ▼]

The cell proliferation-associated antigen Ki 67 and the immunohistochemical content of oestrogen receptors (ER), progesterone receptors AB (PRAB) and progesterone receptors B (PRB) were evaluated in leiomyomata and adjacent myometrium during the menstrual cycle and in leiomyomata under gonadotrophin-releasing hormone agonist (GnRHa) therapy. The proliferative status of muscular cells was measured by evaluating the percentage of nuclei staining positive for Ki 67 (proliferation index). Quantitative analysis (QH-score) was carried out using advanced stereographic computer technology to investigate ER, PRAB and PRB. Leiomyoma and myometrial biopsies were taken from 30 patients undergoing hysterectomy or myomectomy because of symptomatic leiomyomata (subgroup I). Leiomyoma biopsies were taken from 10 patients suffering from symptomatic submucosal leiomyomata, after 2 month GnRH therapy (subgroup II). During the secretory phase, the proliferation index (Ki 67) was found to be higher in leiomyomata than in myometrium, but the difference was not significant. Oestrogen receptor content was significantly higher in leiomyomata than in myometrium only during the proliferative phase of the cycle. PRAB and PRB content were found to be higher in leiomyomata than in adjacent myometrium with a statistically significant dominance of PRAB over PRB. Under GnRHa therapy, a dramatic decrease was observed in PRAB and B content as well as Ki 67 but ER content remained comparable with the results obtained during the menstrual cycle. The results suggest that leiomyomata may be under the influence of progesterone which may play a major role in their growth. [less ▲]

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See detailImmunohistochemical study of the rectus sheath and transversalis facia in adult groin hernias
PANS, Alain ULg; Pierard, Gérald ULg; Albert, Adelin ULg

in Hernia : the Journal of Hernias & Abdominal Wall Surgery (1999), 3

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See detailImmunohistochemical study on cultured FDC-C enriched lymphoid cell populations.
Tsunoda, R.; Cormann, N.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1988), 237

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See detailImmunohistochemical sweat gland profiles.
Noel, Fanchon; Pierard, Gérald ULg; Delvenne, Philippe ULg et al

in Journal of Cosmetic Dermatology (2013), 12

Abstract BACKGROUND: Human sweat glands are heterogeneous in their structures and functions. Accordingly, eccrine, apocrine, and apoeccrine glands are distinguished. AIMS: Some immunohistochemical markers ... [more ▼]

Abstract BACKGROUND: Human sweat glands are heterogeneous in their structures and functions. Accordingly, eccrine, apocrine, and apoeccrine glands are distinguished. AIMS: Some immunohistochemical markers are expected to distinguish the sweat gland types in their secretory and excretory parts. METHODS: This study used two sets of antibodies. The first panel was composed of antibodies directed to well-defined sweat gland structures. The molecular targets included the low-molecular-weight cytokeratins CAM 5.2, the S100-B protein, the epithelial membrane antigen (EMA), the carcinoembryonic antigen (CEA), and the lectin Ulex europaeus agglutinin-1 (UEA-1). A second exploratory panel of antibodies targeted syndecan-1 (CD138), NKI-C3 (CD63), and CD68. They were used to disclose some undescribed antigen expressions in human sweat glands. RESULTS: The first set of antibodies confirmed previous findings. The immunoreactivities of the three sweat gland types were similar in the excretory ducts. By contrast, they were distinguished in the deeper coiled secretory portions of the glands. CONCLUSION: Clues supporting their distinction and probably their functional activity were obtained by immunohistochemistry using the S100-B protein, CEA and CD63 antibodies. The immunoreactivity to the S100-B protein, CEA and CD63 possibly help identifying apoeccrine sweat glands or a peculiar functional activity of eccrine sweat glands. [less ▲]

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See detailImmunohistochemical transforming growth factor Beta1 and its activation, storage and signalling pathways in canine idiopathic pulmonary fibrosis
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailImmunohistological Demonstration Of Virus And Tumor Associated Antigens In Tissues In Experimental And Spontaneous Bovine Leukemia-Virus (Blv) Infection
Reinacher, M.; Thurmond, Mc.; Onuma, M. et al

in Veterinary Immunology and Immunopathology (1989), 22(3),

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See detailImmunohistological localization of three basement membrane components in various forms of epidermolysis bullosa.
Kero, M.; Peltonen, L.; Foidart, Jean-Michel ULg et al

in Journal of Cutaneous Pathology (1982), 9(5), 316-28

The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against ... [more ▼]

The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against collagen Types IV and V, and a proteoglycan. All these molecules are either basement membrane components or closely associated substances. In two types of EB simplex (subtype of the epidermolytic form) the splicing took place above the basement membrane, whereas the staining with all three antibodies remained localized to the floor of the blister. The herpetiform variant of EB simplex proved to be junctional, i.e. the separation occurred within the lamina lucida. One patient clinically classified as belonging to the junctional EB group, was found to have the epidermolytic form of the disease. In this case all antibodies were localized only on the floor of the blister. In the patients with the dermolytic form of EB, all the antibodies stained the roof of the blister. The immunofluorescence techniques are rapid and easy to perform and are therefore proposed as useful for routine clinical diagnosis. [less ▲]

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