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See detailImmunization and Dermatophytes
Mignon, Bernard ULg; Tabart, Jérémy; Baldo, Aline ULg et al

in Current Opinion in Infectious Diseases (2008), 21(2), 134-140

PURPOSE OF REVIEW: Despite the availability of effective vaccines for certain animal species, vaccination against dermatophytosis requires improvement and further development in both animals and humans ... [more ▼]

PURPOSE OF REVIEW: Despite the availability of effective vaccines for certain animal species, vaccination against dermatophytosis requires improvement and further development in both animals and humans. This review provides an update on the current situation and focuses on recent advances in host-dermatophyte relationships that could have implications for future vaccination against the most prevalent of the fungal diseases. RECENT FINDINGS: Numerous dermatophytic virulence factors have recently been isolated and characterized at the molecular level, notably secreted proteases involved in the invasion of the keratin network. Their precise roles in the different steps of the infectious process and in immunopathogenesis are being studied, while all aspects of the host immune response against dermatophytes, including the innate response, are becoming increasingly documented. In addition, new molecular tools are now available for studying dermatophytes, which will accelerate research on this topic. SUMMARY: The growth of knowledge concerning all aspects of the host-dermatophyte relationship should contribute towards sound strategies for the development of effective and safe vaccines against dermatophytosis. [less ▲]

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See detailImmunization and dermatophytes
Baldo, Aline ULg; Tabart, J.; Mathy, Anne ULg et al

Conference (2009)

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See detailImmuno PCR : a new tool for the ultra-sensitive detection of biomolecules
Zorzi, Willy ULg; Elmoualij, Benaïssa ULg

Conference (2008, December 11)

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See detailImmuno-inflammatory mechanisms in refractory asthma
Manise, Maïté ULg

Doctoral thesis (2012)

Detailed reference viewed: 25 (3 ULg)
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See detailThe Immuno-Inflammatory Reaction in Crohn's Disease and Ulcerative Colitis: Characterisation, Genetics and Clinical Application. Focus on Tnf Alpha
Louis, Edouard ULg

in Acta Gastro-Enterologica Belgica (2001), 64(1), 1-5

Inflammatory bowel diseases are multifactorial polygenic diseases. The author has worked on the characterisation of the mucosal immuno-inflammatory reaction, on genetic predisposition and on potential ... [more ▼]

Inflammatory bowel diseases are multifactorial polygenic diseases. The author has worked on the characterisation of the mucosal immuno-inflammatory reaction, on genetic predisposition and on potential clinical application of blood immuno-inflammatory markers in these diseases. This paper summarizes some aspects of this work, focusing on TNF. Following points are developed: production of TNF by inflamed mucosa, genetic control of TNF production, TNF gene polymorphim in inflammatory bowel disease, and evaluation of serum TNF as a marker of disease activity or evolutivity. [less ▲]

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See detailAn immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyond
Ruelle, Virginie ULg; Falisse-Poirier, Nandini; Elmoualij, Benaïssa ULg et al

in Journal of Proteome Research (2007), 6(6), 2168-2175

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies ... [more ▼]

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry ( i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model. [less ▲]

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See detailImmuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
Gofflot, Stéphanie ULg; Elmoualij, Benaïssa ULg; Zorzi, Danièle ULg et al

in Journal of Immunoassay & Immunochemistry (2004), 25(3), 241-258

Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR ... [more ▼]

Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage. [less ▲]

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See detailImmunoassay using a biofunctionnalized alumina-coated capacitive biosensor: towards a microfluidic detection of the H5N1 Influenza virus
van Overstraten-Schlogel, Nancy; Depuis, Pascal; Lefévre, Olivia et al

Poster (2011, December 02)

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See detailImmunoassay using a biofunctionnalized alumina-coated capacitive biosensor: towards a microfluidic detection of the H5N1 Influenza virus
Overstraeten-Schlögel, Nancy; Dupuis, Pascal; Lefévre, Olivia et al

Conference (2011, October 12)

Influenza A viruses cause annual epidemics and occasional pandemics that spread worldwide. The nucleoprotein is essential for the survival of this virus and is thus well-conserved. We developed a ... [more ▼]

Influenza A viruses cause annual epidemics and occasional pandemics that spread worldwide. The nucleoprotein is essential for the survival of this virus and is thus well-conserved. We developed a quantitative electronic biosensor displaying a protein recognition thanks to the covalent grafting of anti-nucleoprotein antibodies. The detection device lies on interdigitated array microelectrodes (IDAM), covered with alumina (Al2O3) to protect the underlying aluminum and enhance the electrical coupling. As the dimensions of the IDAM can determine the sensitivity of the sensor, we processed 4 structures with varying electrode widths and spacings in the same silicon chip and assessed their individual performance. The sensing area of each sensor is 200*200 µm2 and the overall chip-size is 3*3 mm2. The sensor is mounted in a DIL-16-package partially encapsulated by resin. [less ▲]

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See detailImmunobiology of reduced intensity conditioning in hematopoietic stem cell transplantation
Baron, Frédéric ULg; Sandmaier, Brenda

in Hematology education : the education program for the annual congress of the European Hematology Association (2009)

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See detailIMMUNOCAP© ISAC: INTEREST IN ALLERGY DIAGNOSIS
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Poster (2009, November 19)

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See detailImmunoCAP© ISAC: interest in allergy diagnosis
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Conference (2009, April 28)

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See detailImmunocastration of farm animals
Portetelle, Daniel ULg; Haezebroeck, Valérie; Mortiaux, Frédéric et al

in Bases (2000), 4

Detailed reference viewed: 25 (2 ULg)
See detailImmunocastration of farm animals.
Mestdagt, M.; Portetelle, Daniel ULg; Bertozzi, C. et al

in Biotechnology in animal husbandry (2001)

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See detailThe immunochemical determinations of serum lipase in acute pancreatitis: further results.
Adam, A.; Boulanger, J.; Chapelle, Jean-Paul ULg et al

in Clinical Chemistry (1986), 32(10), 1987

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See detailImmunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Rusu, D.; Loret, S.; Peulen, Olivier ULg et al

in BMC Cell Biology (2005), 6

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs ... [more ▼]

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract. Results: Cultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium. Conclusion: The present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations. [less ▲]

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See detailImmunocytochemical demonstration of hormones in cells granules of several human pituitary adenomas
Beckers, Albert ULg; Stevenaert, Achille ULg; Courtoy, R. et al

in Histochemical Journal (The) (1986)

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