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See detailImportance Of The Hydrophobic Energy: Structural Determination Of A Hypoglycemic Drug Of The Meglitinide Family By Nuclear Magnetic Resonance And Molecular Modeling
Lins, Laurence ULg; Brasseur, Robert ULg; Malaisse, Wj. et al

in Biochemical Pharmacology (1996), 52(8), 1155-68

The molecular structure of (2S)-2-benzyl-3-(cis-hexahydro-2-isoindolinylcarbonyl) propionic acid (KAD-1229), a hypoglycemic drug of the meglitinide family, was studied by nuclear magnetic resonance (NMR ... [more ▼]

The molecular structure of (2S)-2-benzyl-3-(cis-hexahydro-2-isoindolinylcarbonyl) propionic acid (KAD-1229), a hypoglycemic drug of the meglitinide family, was studied by nuclear magnetic resonance (NMR) and molecular modeling. The results of the NMR experiments indicated that KAD-1229 existed in solution in the form of two stable conformers of equal population, called KADI and KADII in this paper. Three different molecular modelings were then applied: the classical molecular dynamics using the commercial Biosym and Hyperchem softwares and the Prot+ program, which is not based on a dynamical study but on a systematic conformational analysis of the molecule, which includes a term that allows the estimation of the hydrophobic interaction. The modeling results showed the following points. First, in contrast with classical molecular dynamics, which uses restraints from two-dimensional nuclear Overhauser effect (NOE) data, the Prot+ KAD structure provides conformations that support experimental NMR data without any external intervention. In the structures in agreement with NMR data, an important hydrophobic interaction between the phenyl cycle and the perhydroisoindole ring of KAD is observed. This interaction, which seems to play a role in the biological activity of the drug, is lost when no restraints are considered in classical molecular dynamics. Second, the difference between KADI and KADII arises mainly from slight distance geometric differences at the level of the perhydroisoindole and the phenyl rings. [less ▲]

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See detailImportance of the microbenthic loop of Posidonia oceanica meadows to detect anthropogenic perturbations early: first results
Pete, Dorothée ULg; Velimirov, Branko; Bouquegneau, Jean-Marie ULg et al

Conference (2008, August)

It was demonstrated that Posidonia oceanica, the marine magnoliophyte endemic to the Mediterranean Sea, is a valuable tool to assess environmental qualities of the coastal zones. However, only few studies ... [more ▼]

It was demonstrated that Posidonia oceanica, the marine magnoliophyte endemic to the Mediterranean Sea, is a valuable tool to assess environmental qualities of the coastal zones. However, only few studies have attempted to use characteristics of its sediment compartment as an indicator of the environment state of health. Yet, organisms living in this compartment have a turnover which is faster than for canopy-organisms which makes them useful as valid early descriptors of pollution. The study described here takes place in a project which aims to find an early holistic indicator of anthropogenic perturbations in the Mediterranean coastal zones. The investigation is based on the microbenthic loop (organic matter, bacteria, microphytobenthos and meiofauna) and was led in two different sites of Calvi Bay (Corsica, France), at 10 m depth, in March and June 2007. Only results about bacteria (abundance, biomass, morphotypes), microphytobenthos (biomass) and organic matter (biomass) will be presented. For both seasons, significant differences between sites are found, irrespective on whether variables are treated separately or together, thus indicating that the microbenthic loop has potential to be a good early indicator of pollution. [less ▲]

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See detailThe Importance of the Negative Charge of Beta-Lactam Compounds in the Interactions with Active-Site Serine Dd-Peptidases and Beta-Lactamases
Varetto, Louis ULg; De Meester, Fabien; Monnaie, Didier et al

in Biochemical Journal (1991), 278(Pt 3), 801-807

The interaction between various penicillins and cephalosporins the carboxylate group of which at C-3 or C-4 had been esterified or amidated and different penicillin-recognizing enzymes was studied. In ... [more ▼]

The interaction between various penicillins and cephalosporins the carboxylate group of which at C-3 or C-4 had been esterified or amidated and different penicillin-recognizing enzymes was studied. In general, our findings reinforced the common assumption that an anionic group at that position is necessary for the effective acylation of these enzymes. However, the relative activities of the modified beta-lactams as inactivators of the Streptomyces R61 DD-peptidase or as substrates of the Bacillus licheniformis, Streptomyces albus G and Enterobacter cloacae beta-lactamases did not fit a general scheme in which the intrinsic electronic and geometric properties of the beta-lactam compounds would be sufficient to explain their substrate or inactivator properties towards the various types of enzymes investigated. [less ▲]

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See detailThe importance of the negative charge of β-lactam compounds for the inactivation of the active-site serine DD-peptidase of Streptomyces R61
Varetto, Louis ULg; Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg

in FEBS Letters (1987), 225(1-2), 218-222

The interaction between the Streptomyces R61 penicillin-sensitive DD-peptidase and deacetyl-cephalosporin C or its lactone derivative has been studied at different pH values. The results show the ... [more ▼]

The interaction between the Streptomyces R61 penicillin-sensitive DD-peptidase and deacetyl-cephalosporin C or its lactone derivative has been studied at different pH values. The results show the importance of an enzyme group of pK approximately equal to 9 which might form an ion pair with the free carboxylate of the former compound. This electrostatic interaction is shown to contribute to the formation of the first, non-covalent enzyme-inactivator complex by a factor of at least 50. [less ▲]

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See detailImportance of the PIKKs in NF-kappaB activation by genotoxic stress
Sabatel, Hélène ULg; Pirlot, Céline ULg; Piette, Jacques ULg et al

in Biochemical Pharmacology (2011), 82(10), 1371-83

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See detailImportance of the Two Tryptophan Residues in the Streptomyces R61 Exocellular Dd-Peptidase
Bourguignon-Bellefroid, Catherine; Wilkin, Jean-Marc; Joris, Bernard ULg et al

in Biochemical Journal (1992), 282(Pt 2), 361-367

Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzyme's two tryptophan residues was investigated by ... [more ▼]

Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzyme's two tryptophan residues was investigated by site-directed mutagenesis. Trp271 was replaced by Leu. The modification yielded a stable enzyme whose structural and catalytic properties were similar to those of the wild-type protein. Thus the Trp271 residue, though almost invariant among the beta-lactamases of classes A and C and the low-Mr penicillin-binding proteins, did not appear to be essential for enzyme activity. Mutations of the Trp233 into Leu and Ser strongly decreased the enzymic activity, the affinity for beta-lactams and the protein stability. Surprisingly, the benzylpenicilloyl-(W233L)enzyme deacylated at least 300-fold more quickly than the corresponding acyl-enzyme formed with the wild-type protein and gave rise to benzylpenicilloate instead of phenylacetylglycine. This mutant DD-peptidase thus behaved as a weak beta-lactamase. [less ▲]

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See detailImportance of Tumor Pathology
Beckers, Albert ULg

Scientific conference (2001, June)

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See detailImportance of Tumor Regression Assessment in Predicting the Outcome in Patients with Locally Advanced Rectal Carcinoma Who Are Treated with Preoperative Radiotherapy
Bouzourene, Hanifa; Bosman, Fred; Seelentag, Walter et al

in Cancer (2002), 94(4), 1121-1130

BACKGROUND: Locally advanced rectal carcinoma has a poor prognosis. However, <br />since the introduction of preoperative radiotherapy, the outcome of patients with <br />rectal carcinoma has been ... [more ▼]

BACKGROUND: Locally advanced rectal carcinoma has a poor prognosis. However, <br />since the introduction of preoperative radiotherapy, the outcome of patients with <br />rectal carcinoma has been reported to have improved. Nevertheless, to the authors’ <br />knowledge few data are available regarding the histopathologic response to <br />radiotherapy as assessed on surgical specimens as a potential predictive factor for <br />outcome. <br />METHODS: To estimate the effect of radiotherapy on rectal carcinoma, the authors <br />retrospectively reviewed the surgical specimens of 102 patients with T3-4, N0 or <br /> N1 rectal carcinoma and 1 patient with T2 but N1 rectal carcinoma. All patients <br />were treated preoperatively with a hyperfractionated accelerated radiotherapy <br />schedule in a prospective protocol (Trial 93-01). Using a standardized approach, <br />tumor regression was graded using a system that varies from Grade 1 (tumor <br />regression Grade [TRG] 1) when complete tumor regression is observed to Grade 5 <br />(TRG5) when no tumor regression is observed. <br />RESULTS: Radiotherapy resulted in tumor downstaging in 43% of the patients. <br />There were 2 pT1 tumors (2%), 21 pT2 tumors (20%), 66 pT3 tumors (64%), and 14 <br />pT4 tumors (14%) after treatment. Regional lymph nodes were involved in 55 <br />patients (53%). None of the patients demonstrated a complete tumor regression <br />after radiotherapy, but in 79% of the specimens a partial tumor regression was <br />observed (TRG1: 0%; TRG2: 20%; TRG3: 39%; TRG4: 20%; and TRG5: 21%). The <br />median actuarial overall survival (OS) and disease-free survival (DFS) were 52 <br />months. Actuarial local recurrence rates at 2 years and 5 years were 6.4% and 7.6%, <br />respectively. Univariate analysis showed the actuarial DFS to be significantly lower <br />in patients with lymph node metastases (P 0.0004) and advanced pT stages <br />(pT3-4) (P 0.03). A favorable outcome for OS, DFS, and local control was <br />observed in patients with TRG2-4 (i.e., responders) compared with patients with <br />TRG5 (i.e., nonresponders), but also in patients with low residual tumor cell <br />density (TRG2, 3, and 4). On multivariate analysis, TRG remained an independent <br />prognostic indicator for local tumor control. <br />CONCLUSIONS. Tumor regression as well as residual tumor cell density were found <br />to be predictive factors of survival in rectal carcinoma patients after preoperative <br />radiotherapy. Even after preoperative radiotherapy, the pathologic stage of the <br />surgical specimen remained a prognostic factor. The use of a standardized approach <br />for pathologic evaluation must be implemented to allow comparison between <br />the results of various treatment approaches. [less ▲]

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See detailImportance of Weight Loss and Sodium Restriction in the Treatment of Mild and Moderate Essential Hypertension
Krzesinski, Jean-Marie ULg; Janssens, Marion; Vanderspeeten, Francine et al

in Acta Clinica Belgica (1993), 48(4), 234-245

The effectiveness of non pharmacological treatment of chronic arterial hypertension has been evaluated in 116 treated or not primary hypertensive out-patients. Those receiving diuretics were however ... [more ▼]

The effectiveness of non pharmacological treatment of chronic arterial hypertension has been evaluated in 116 treated or not primary hypertensive out-patients. Those receiving diuretics were however excluded. This population was requested to follow for 3 months a modification of their usual diet characterized by salt restriction combined with energy intake restriction when the patients were above their ideal body weight. Only 62 patients (53%) completed the study. However, this approach was effective by decreasing blood pressure and total cholesterol. The weight loss appears the most effective way to decrease blood pressure in untreated patients, but needs 3 months to be significant. In drug-treated patients, the sodium restriction significantly influences the blood pressure level, already after 1 month. However, salt sensitivity has been noted, especially in the most severe forms of the hypertensive disease. The combination of both modifications (weight loss and sodium restriction) does not appear to be more effective than each separate dietary measure. [less ▲]

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See detailL'importance stratégique de la Belgique avant la Première Guerre mondiale
Bechet, Christophe ULg

Scientific conference (2013, November 12)

Présentation des principaux résultats de notre thèse à un groupe d'étudiants de master en Histoire

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See detailL’importance stratégique des chemins de fer belges avant la Première Guerre mondiale. Un enjeu international
Bechet, Christophe ULg

in Société archéologique de Namur (Ed.) Actes du LVème Congrès de la Fédération des Cercles d’Archéologie et d’Histoire de Belgique – Namur 2008 (2011)

Schlieffen réfléchit à la possibilité d’un passage par la Belgique depuis fin 1896 et la première trace écrite d’une stratégie de débordement de l’aile gauche française par le nord se trouve dans un ... [more ▼]

Schlieffen réfléchit à la possibilité d’un passage par la Belgique depuis fin 1896 et la première trace écrite d’une stratégie de débordement de l’aile gauche française par le nord se trouve dans un Denkschrift rédigé par lui le 2 août 1897. Toutefois, à ce moment, il n’est pas encore question pour Schlieffen de concrétiser ses réflexions dans le plan allemand officiel car il lui faut vaincre deux obstacles majeurs. Primo, la nécessité de créer un nombre de corps d’armée suffisant pour que la réalisation du mouvement tournant par la Belgique se fasse sans trop dégarnir les fronts d’Alsace-Lorraine et de Russie. Secundo, Schlieffen ne peut aligner de nombreuses divisions face à la Belgique que grâce à un réseau ferroviaire suffisamment développé dans l’Eiffel allemand. Le développement des lignes de chemin de fer dans la zone frontalière belgo-allemande offre de nouvelles perspectives stratégiques en cas de guerre future. [less ▲]

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See detailL’importance stratégique du territoire belge jugée par les attachés militaires anglais en poste à Bruxelles (1891-1914)
Bechet, Christophe ULg

Scientific conference (2012, March 06)

La série de rapports militaires encore présents dans la correspondance de la légation britannique à Bruxelles est incomplète et moins riche que les nombreux rapports conservés aux archives de l’Armée de ... [more ▼]

La série de rapports militaires encore présents dans la correspondance de la légation britannique à Bruxelles est incomplète et moins riche que les nombreux rapports conservés aux archives de l’Armée de Terre à Vincennes ; il n’empêche, le nombre de documents existants demeure important et susceptible de livrer des informations de première main sur les relations militaires belgo-britanniques avant la Première Guerre mondiale. Notre intervention consistera à faire un état des lieux des documents conservés, de leur localisation et de ce qu’ils peuvent nous apprendre sur la perception britannique du territoire belge. A l’aide des recherches que nous avons réalisées dans le cadre de notre thèse sur les plans français et allemands dans leurs rapports avec la Belgique, nous élargirons nos réflexions sur les relations belgo-britanniques dans une perspective militaire européenne. [less ▲]

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See detailImportances des profils phénotypiques dans la prise en charge des enfants présentant des retards mentaux
Comblain, Annick ULg

Conference (2001, September 28)

Les difficultés d'acquisition et de développement du langage oral des personnes retardées mentales, et plus particulièrement, de celles atteintes d'un retard mental d'origine génétique, sont, à l'heure ... [more ▼]

Les difficultés d'acquisition et de développement du langage oral des personnes retardées mentales, et plus particulièrement, de celles atteintes d'un retard mental d'origine génétique, sont, à l'heure actuelle, reconnues mais malheureusement encore mal connues. Il existe en effet une grand variabilité inter- et intra-syndromes qu'il est aujourd'hui important d'étudier et ce dans une perspective d'intervention adaptée et efficace. Les recherches menés depuis quelques années dans le domaine de la spécificité syndromique suggèrent que les développements et les fonctionnements cognitif, langagier, mnésique, perceptuel et attentionnel diffèrent considérablement d'un syndrome à l'autre. Jusqu'au début des années 1990, on envisageait le développement des personnes présentant un retard mental (plus particulièrement modéré) en référence à un modèle dit "prototypique" : le syndrome de Down ou trisomie 21. [less ▲]

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See detailImportancia de la elección de distintos oligonucleótidos para el diagnostico de BLV por PCR en productos de origen bovino
Trono, K.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2006, December 05)

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See detailImportant differences between multiple offenders ans non-multiple offenders
Gavray, Claire ULg

Conference (2012, September 13)

importance to distinguish between young people who have a 'single- light' experience' of crime and those who do repeat illegal behaviours over time. The profile of those young people is quite different ... [more ▼]

importance to distinguish between young people who have a 'single- light' experience' of crime and those who do repeat illegal behaviours over time. The profile of those young people is quite different and the necessary prevention is specific in both cases [less ▲]

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See detailImportant differences in management policies for children with end-stage renal disease in the Netherlands and Belgium--report from the RICH-Q study.
Tromp, Wilma F.; Schoenmaker, Nikki J.; van der Lee, Johanna H. et al

in Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (2012), 27(5), 1984-92

BACKGROUND: The low prevalence of childhood end-stage renal disease and the small centre sizes have been a barrier for clinical studies and the development of evidence-based guidelines for chronic renal ... [more ▼]

BACKGROUND: The low prevalence of childhood end-stage renal disease and the small centre sizes have been a barrier for clinical studies and the development of evidence-based guidelines for chronic renal replacement therapy (cRRT) in children. Few data exist on the quality of care for these patients and the applicability of existing guidelines. The aim of this study is to quantify variation in treatment policies and actually delivered care in nine centres that deliver cRRT for children. METHODS: We surveyed treatment policies in all nine centres in the Netherlands and Belgium and compared them with the actually provided therapies and with recommendations from available guidelines. Data on treatment policies were gathered by questionnaires; actually provided care and outcomes were registered prospectively from 2007 to 2010. RESULTS: Data on policies and actual patient care were obtained from all nine centres. We found relevant differences between centres in treatment policies on various topics, e.g. estimated glomerular filtration rate threshold as an indication for initiation of cRRT, preferred initial mode of cRRT, peritoneal dialysis catheter care, haemodialysis frequency and vascular access. Discrepancies were seen between stated treatment policies and actual performed therapies. For the majority of policies, no evidence-based guidelines are available. CONCLUSIONS: Health care disparities exist due to large and unwanted variation in treatment policies between hospitals providing cRRT for children. Delivered care does not live up to stated policies, for which clear and internationally accepted guidelines are lacking. [less ▲]

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See detailImportant role of the mid-tropospheric atmospheric circulation in the recent surface melt increase over the Greenland ice sheet
Fettweis, Xavier ULg; Hanna, Edward; Lang, Charlotte ULg et al

in Cryosphere (The) (2013), 7

Since 2007, there has been a series of surface melt records over the Greenland ice sheet (GrIS), continuing the trend towards increased melt observed since the end of the 1990's. The last two decades are ... [more ▼]

Since 2007, there has been a series of surface melt records over the Greenland ice sheet (GrIS), continuing the trend towards increased melt observed since the end of the 1990's. The last two decades are characterized by an increase of negative phases of the North Atlantic Oscillation (NAO) favouring warmer and drier summers than normal over GrIS. In this context, we use a circulation type classification based on daily 500 hPa geopotential height to evaluate the role of atmospheric dynamics in this surface melt acceleration for the last two decades. Due to the lack of direct observations, the interannual melt variability is gauged here by the summer (June–July–August) mean temperature from reanalyses at 700 hPa over Greenland; analogous atmospheric circulations in the past show that ~70% of the 1993–2012 warming at 700 hPa over Greenland has been driven by changes in the atmospheric flow frequencies. Indeed, the occurrence of anticyclones centred over the GrIS at the surface and at 500 hPa has doubled since the end of 1990's, which induces more frequent southerly warm air advection along the western Greenland coast and over the neighbouring Canadian Arctic Archipelago (CAA). These changes in the NAO modes explain also why no significant warming has been observed these last summers over Svalbard, where northerly atmospheric flows are twice as frequent as before. Therefore, the recent warmer summers over GrIS and CAA cannot be considered as a long-term climate warming but are more a consequence of NAO variability affecting atmospheric heat transport. Although no global model from the CMIP5 database projects subsequent significant changes in NAO through this century, we cannot exclude the possibility that the observed NAO changes are due to global warming. [less ▲]

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See detailImportant roles of SoHo proteins in vascular development: Sorbs1 and Sorbs2 have related but distinct functions in endothelial cells angiogenic properties
Bleuart, Anouck ULg

Doctoral thesis (2014)

Vascular system in all vertebrate organisms has in charge essential functions such as to bring nutrients, oxygen and signalling molecules to distant organs. Vascular network is established through ... [more ▼]

Vascular system in all vertebrate organisms has in charge essential functions such as to bring nutrients, oxygen and signalling molecules to distant organs. Vascular network is established through sequential and distinct processes. During embryogenesis, angioblasts give rise to differentiated endothelial cells, which form primary vessels through vasculogenesis. Expansion of this primary network is then achieved through a multistep process known as angiogenesis. During angiogenesis, remodelling of endothelial cell shape relies on cytoskeleton dynamics. In this study, our goal was to investigate the potential role of the SoHo family of adaptor proteins in angiogenesis. The SoHo family comprises three members, Sorbs1, Sorbs2 and Sorbs3. Although accumulative evidence links this protein family to the regulation of cell adhesion and actin cytoskeletal organisation, their expression and potential functions in endothelial cells remain unknown. Using zebrafish loss of function experiments combined with confocal imaging, we show that loss of Sorbs1 affects caudal vein-derived vessel formation, whereas Sorbs2 depletion impairs ISV development essentially derived from the dorsal aorta. We then demonstrated that both members impact on EC behaviour via regulation of RhoGTPases activity, leading to the vascular defects observed in vivo. Using a series of in vitro functional assays, we found that Sorbs1 and Sorbs2 have opposite effects on adhesives properties of HUVECs related to their ability to regulate different RhoGTPases. Sorbs1 depleted ECs adhere excessively to the ECM due to decrease in Rac1 activity. In contrast, loss of Sorbs2 impaired RhoA activation during adhesion and decreases cell adhesiveness to the matrix. Interestingly, these opposite effects on cell adhesion lead to similar outcome on EC migratory abilities, a decrease in cell migration. Here, for the first time, we establish a role for Sorbs1 and Sorbs2 in angiogenesis and demonstrate that these SoHo members are essential for EC adhesiveness and subsequent migratory abilities. [less ▲]

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See detailImportant spores for the delineation of the Devonian-Carboniferous Boundary through USSR and Western Europe.
Chibrikova, EV; Kedo, GI; Streel, Maurice ULg

in Compte Rendu 8ème Congrès international de Stratigraphie et de Géologie du Carbonifère. Moscou 1975, 1 (1978)

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