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See detailImmunocytochemical study of subcellular distribution of bovine leukaemia virus regulatory Tax protein
Russev, Russi; Shikova, E.; Portetelle, Daniel ULg et al

Poster (2002, October)

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See detailImmunocytochemical study of subcellular distribution of bovine leukaemia virus regulatory Tax protein.
Russev, R.; Shikova, E.; Portetelle, Daniel ULg et al

in Annals of Oncology (2002), 13(2), 14

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See detailImmunocytochemical techniques reveal new apects in trypanosomatids which harbour endosymbionts
Motta, M C M; De Souza, W; Thiry, Marc ULg

in Memorias do Instituto Oswaldo Cruz (1998), 93

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See detailImmunocytochemistry of lipids : chemical fixatives have dramatic effects on the preservation of tissue lipids
Maneta-Peyret, Lilly; Compère, Philippe ULg; Moreau, Patrick et al

in Histochemical Journal (The) (1999), 31

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See detailImmunocytochemistry of the golden hamster pituitary during chronic administration of diethylstilbestrol: a quantitative analysis using laser scanning microscopy
Van Cauwenberge, A.; Nonclercq, D.; Laurent, G. et al

in Histochemistry & Cell Biology (2001), 115

The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to ... [more ▼]

The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to diethylstilbestrol (DES). Cell proliferation in the hypophysis was monitored by the immunohistochemical demonstration of S-phase cells after pulse labeling with 5-bromo-2'-deoxyuridine. Cell proliferation analysis was combined with the identification of different cell populations by immunostaining with antisera raised against hypophyseal hormones. Sections processed for double-label immunofluorescence were examined by confocal microscopy. In the adenohypophysis, the relative surface occupied by gonadotrophs and thyrotrophs decreased rapidly during the first months of treatment while corticotroph and somatotroph populations remained unaffected. Accordingly, the incidence of S-phase cells in these four cell populations was lower than or similar to control values. In contrast, lactotrophs increased gradually during the first month of exposure to DES to reach a maximum value at 2-4 months. At the beginning, this increase was primarily due to hyperplasia but later on it also involved cellular hypertrophy. Somatomammotrophs did not seem to be involved in this model. In the pars intermedia, the labeling index of melanotrophs rose rapidly to reach values 5-6 times higher than controls. After 4 months, neoplasms originating from the pars intermedia were seen invading both the neuro- and the adenohypophysis. At the end of treatment, the pituitary was markedly enlarged resulting from the development of an adenoma of the pars intermedia [less ▲]

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See detailImmunocytochimie et diagnostic des leucémies aigües: mise au point d'une technique utilisant le complexe phosphatase alcaline-anticorps anti-phosphatase alcaline.
Hayette, Marie-Pierre ULg

Master of advanced studies dissertation (1990)

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure ... [more ▼]

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure pas moins vrai que l'introduction des anticorps (Ac) monoclonaux a ouvert des perspectives nouvelles en associant des profils immunologiques aux différents types de leucémies aiguës. Des techniques immunocytochimiques utilisant différents enzymes, phosphatases alcaline et peroxydase, associés à des systèmes d'amplification, complexes phosphatase alcaline anticorps anti-phosphatase alcaline (APAAP) ou peroxydase anticorps anti-peroxydase (PAP) ou avidine-biotine peroxydase (ABC), permettent de travailler directement sur frottis sanguins ou médullaires. Ce travail propose une mise au point de la technique à l'APAAP sur lame, afin de répondre au problème posé par les moelles pauvres en blastes dont l'étude est difficile à réaliser par immunofluorescence en cytométrie de flux. [less ▲]

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See detailImmunodetection of bovine chorionic somato-mammotrophin (bCS)
Verstegen, J.; Fellmann, D.; Beckers, Jean-François ULg

in Acta Endocrinologica (1985), 109

Bovine chorionic somatomammotrophin (bCS) was detected in the bovine placenta by using an immunoenzymatic procedure. This hormone appears mainly located in teh binucleate cells of the foetal cotyledons ... [more ▼]

Bovine chorionic somatomammotrophin (bCS) was detected in the bovine placenta by using an immunoenzymatic procedure. This hormone appears mainly located in teh binucleate cells of the foetal cotyledons and to a lesser extent in some localized superficial areas of the foetal binucleate cells migrating into the maternal caruncles. [less ▲]

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See detailImmunodetection of RNA on ultra-thin sections incubated with polyadenylate nucleotidyl transferase.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1993), 41(5), 657-65

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and ... [more ▼]

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and biotinylated ATP. The labeled nucleotides bound to RNA at the surface of the ultra-thin sections were than visualized by an indirect immunogold labeling technique. The resulting labeling pattern was dependent on the presence of divalent cations in the PnT medium. The method revealed with great precision the specific RNA-containing structures within Ehrlich tumor cells. The method is applicable to Epon sections. However, the labeling intensity varies according to the fixation used. Best results were obtained on acetylated cell sections. The method can be combined with EDTA regressive staining. The in situ PnT method provides a very useful tool for pinpointing the precise location of RNA within biological material at the ultrastructural level. [less ▲]

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See detailImmunodetection of the metastasis-associated laminin receptor in human breast cancer cells obtained by fine-needle aspiration biopsy.
Castronovo, Vincenzo ULg; Colin, Claude; Claysmith, A. P. et al

in American Journal of Pathology (1990), 137(6), 1373-81

Fine-needle aspiration biopsy of the breast is a very useful technique for the evaluation of a suspect lesion before surgical removal. Increased expression of the 67-kd laminin receptor has been ... [more ▼]

Fine-needle aspiration biopsy of the breast is a very useful technique for the evaluation of a suspect lesion before surgical removal. Increased expression of the 67-kd laminin receptor has been associated with the metastatic phenotype of cancer cells, particularly in colon and breast cancers. In this study, the expression of laminin receptor was evaluated using the immunoperoxidase technique in 81 breast aspirates (26 benign and 55 neoplastic lesions). Cells obtained from benign samples exhibited a low level of laminin receptor antigen detected by affinity-purified antibody raised against a cDNA-derived laminin receptor peptide. In contrast, 71% of smears obtained from malignant breast lesions contained cells that were strongly stained by the antibody. Heterogeneous expression of the laminin receptor was noted in both breast aspirates and fixed tissue specimens. These data suggest that the immunodetection of laminin receptor in cells obtained by fine-needle aspiration of breast lesions could be a valuable adjunct in the prognostic evaluation of breast lesions. [less ▲]

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See detailLes immunodosages
Elmoualij, Benaïssa ULg

Learning material (2011)

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See detailImmunoelectron microscope detection of newly synthetized RNA in isolated nucleoli of Ehrlich tumor cells
Thiry, Marc ULg; Vandelaer, M; Goessens, G

Poster (1995)

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See detailImmunoelectron microscope localization of bromodeoxyuridine incorporated into DNA of Ehrlich tumor cell nucleoli.
Thiry, Marc ULg

in Experimental Cell Research (1988), 179(1), 204-13

The distribution of DNA within the nucleolus of Ehrlich tumor cells has been investigated by means of a recent immunocytochemical approach involving an electron microscopic detection of incorporated 5 ... [more ▼]

The distribution of DNA within the nucleolus of Ehrlich tumor cells has been investigated by means of a recent immunocytochemical approach involving an electron microscopic detection of incorporated 5-bromodeoxyuridine (BUdR) into DNA by an anti-BUdR monoclonal antibody. An immunogold method has been performed on ultrathin sections of cells embedded in Lowicryl K4M. In the nucleolus, gold particles are essentially found over the perinucleolar chromatin adn over its intranucleolar invaginations which are connected with the fibrillar centers. In addition, a few gold particles are also observed in the fibrillar centers, preferentially toward their peripheral regions. In contrast, the dense fibrillar component is completely devoid of labeling. The results are discussed in the context of other recent findings concerning the functional organization of the nucleolus. [less ▲]

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See detailImmunoelectron microscope localization of DNase I sensitive sequences within the cell nucleus
Thiry, Marc ULg

in Cell Biology International Reports (1990), 14

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See detailImmunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumor cells.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in European Journal of Cell Biology (1988), 47(2), 346-57

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU ... [more ▼]

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus. [less ▲]

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See detailImmunoendocrinology in Health and Disease
Geenen, Vincent ULg; Chrousos, George

Book published by Marcel Dekker (2004)

L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires ... [more ▼]

L'objectif de cet ouvrage est d'illustrer l'importance de l'immunoendocrinologie pour la compréhension de la pathogénie et le traitement des endocrinopathies auto-immunes, et des maladies inflammatoires, infectieuses, allergiques et néoplasiques, de même que le processus d'immunosénescence. Des experts internationaux de chaque domaine ont rédigé des chapitres à propos de maladies chroniques qui ont un impact majeur tant émotionnel qu'économique sur la société humaine. [less ▲]

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See detailImmunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
Kimata, K.; Foidart, Jean-Michel ULg; Pennypacker, J. P. et al

in Cancer Research (1982), 42(6), 2384-91

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has ... [more ▼]

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation. [less ▲]

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