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See detailImmunocytochemical demonstration of hormones in cells granules of several human pituitary adenomas
Beckers, Albert ULg; Stevenaert, Achille ULg; Courtoy, R. et al

in Histochemical Journal (The) (1986)

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See detailImmunocytochemical demontration of hormones in cell granules of several human pituitary adenomas
Beckers, Albert ULg; Courtoy, R.; Stevenaert, Achille ULg et al

in Histochemical Journal (The) (1986), 18(1), 55

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See detailImmunocytochemical detection of DNA and RNA in endosymbiont-bearing trypanosomatids.
Motta, Maria Cristina M; de Souza, Wanderley; Thiry, Marc ULg

in FEMS Microbiology Letters (2003), 221(1), 17-23

Research about the kinetoplast of trypanosomatids has yielded valuable information about the organization of extranuclear structure. However, the ultrastructural localization of nucleic acids within these ... [more ▼]

Research about the kinetoplast of trypanosomatids has yielded valuable information about the organization of extranuclear structure. However, the ultrastructural localization of nucleic acids within these protozoa remains uncertain. We have applied cytochemical and immunocytochemical approaches to precisely identify DNA and RNA in lower endosymbiont-bearing trypanosomatids. Using the Terminal deoxynucleotidyl Transferase (TdT) immunogold technique, we showed that nuclear DNA is seen associated with the nuclear envelope during the trypanosomatid cell cycle. By combining the TdT technique with the acetylation method, which improves the contrast between structures containing fibrils and granules, we have demonstrated that the nucleolus of endosymbiont-bearing trypanosomatids is composed of two constituents: a granular component and a DNA-positive fibrillar zone. Moreover, we revealed that DNA of endosymbiotic bacteria consisted of electron-dense filaments which are usually in close contact with the prokaryote envelope. Using a Lowicryl post-embedding immunogold labeling procedure with anti-RNA antibodies, we showed the presence of RNA not only over the cytoplasm, the interchromatin spaces and the nucleolus, but also over the kinetoplast and virus-like particles present in Crithidia desouzai. [less ▲]

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See detailImmunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
Bastings, E.; Beckers, Albert ULg; Reznik, Michel ULg et al

in Biology of Reproduction (1991), 45(5), 788-96

In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based ... [more ▼]

In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LH in secretory granules of gonadotrophs using highly specific antisera. This morphological study included light microscopy (PAP) and electron microscopy (immunogold single and double labeling) procedures. Histologically, approximatively 11.5% of cells were positive for LH, whereas only 5.4% of cells were positive for FSH. With the electron microscope, single labeling allowed identification of morphologically distinct LH-containing cells and FSH-containing cells. Double immunostaining confirmed that no cells contained both hormones. The finding that FSH and LH are produced in separate pituitary cells is in agreement with recent studies that have suggested a specific role and regulatory process for gonadotropins in the bovine species. [less ▲]

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See detailImmunocytochemical evidence that LH and FSH are produced by distinct cells in bovine pituitary.
Bastings, E.; Beckers, Albert ULg; Reznik, M. et al

in Theriogenology January (1990)

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See detailImmunocytochemical Localization of Androgen Receptors in the Male Songbird and Quail Brain
Balthazart, Jacques ULg; Foidart, Agnès ULg; Wilson, E. M. et al

in Journal of Comparative Neurology (The) (1992), 317(4), 407-20

The distribution of androgen receptors was studied in the brain of the Japanese quail (Coturnix japonica), the zebra finch (Taeniopygia guttata), and the canary (Serinus canaria) by immunocytochemistry ... [more ▼]

The distribution of androgen receptors was studied in the brain of the Japanese quail (Coturnix japonica), the zebra finch (Taeniopygia guttata), and the canary (Serinus canaria) by immunocytochemistry with a polyclonal antibody (AR32) raised in rabbit against a synthetic peptide corresponding to a sequence located at the N-terminus of the androgen receptor molecule. In quail, androgen receptor-immunoreactive cells were observed in the nucleus intercollicularis and in various nuclei of the preoptic-hypothalamic complex, namely, the nucleus preopticus medialis, the ventral part of the nucleus anterior medialis hypothalami, the nucleus paraventricularis magnocellularis, the nucleus ventromedialis hypothalami, and the tuberal hypothalamus. In the two songbird species, labeled cells were also observed in various nuclei in the preoptic-hypothalamic region, in the nucleus taeniae, and in the nucleus intercollicularis. Additional androgen receptor-immunoreactive cells were present in the androgen-sensitive telencephalic nuclei that are part of the song control system. These immunoreactive cells filled and outlined the boundaries of the hyperstriatum ventrale, pars caudalis, nucleus magnocellularis neostriatalis anterioris (both in the lateral and medial subdivisions), and nucleus robustus archistriatalis. The immunoreactive material was primarily present in cell nuclei but a low level of immunoreactivity was also clearly detected in cytoplasm in some brain areas. These studies demonstrate, for the first time, that androgen receptors can be detected by immunocytochemistry in the avian brain and the results are in general agreement with the binding data obtained by autoradiography with tritiated dihydrotestosterone. Immunocytochemical methods offer several advantages over autoradiography and their use for the study of the androgen receptor will greatly facilitate the analysis of steroid-sensitive systems in the avian brain. [less ▲]

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See detailImmunocytochemical localization of aromatase in sensory and integrating nuclei of the hindbrain in Japanese quail (Coturnix japonica)
Evrard, H. C.; Harada, N.; Balthazart, Jacques ULg

in Journal of Comparative Neurology (2004), 473(2), 194-212

The distribution of the estrogen synthesizing enzyme (aromatase) in the hindbrain (rhombencephalon and mesencephalon) of male adult quail was investigated by immunocytochemistry. Aromatase-immunoreactive ... [more ▼]

The distribution of the estrogen synthesizing enzyme (aromatase) in the hindbrain (rhombencephalon and mesencephalon) of male adult quail was investigated by immunocytochemistry. Aromatase-immunoreactive neuronal structures (perikarya and fibers bearing punctate structures) were observed in sensory (trigeminal, solitary tract, vestibular, optic tectum) and integrating (parabrachial, periaqueductal, cerulean, raphe) nuclei. Besides the expression of aromatase in these well-delineated nuclei, dense to scattered networks of immunoreactive fibers were found dispersed throughout the hindbrain and, in particular, in its rostral and dorsal parts. To a lesser extent, they were also present throughout the premotor nuclei of the reticular formation and in various fiber tracts. In contrast, no immunoreactive signal was found in motor nuclei, and in most of the statoacoustic (cerebellum, cochlear, olive, pontine, part of vestibular) nuclei. The expression of aromatase in perikarya and fibers in areas of the adult hindbrain where estrogen receptors have been identified previously suggests a role for estrogens locally produced in the regulation of sensory and integrating functions, contrary to the widespread assumption that these functions are regulated exclusively by steroids produced in the gonads. (C) 2004 Wiley-Liss, Inc. [less ▲]

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See detailImmunocytochemical Localization of Aromatase in the Brain
Balthazart, Jacques ULg; Foidart, Agnès ULg; Harada, N.

in Brain Research (1990), 514(2), 327-33

An immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the Japanese ... [more ▼]

An immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the Japanese quail brain. Immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the sexually dimorphic medial preoptic nucleus, in the ventromedial nucleus of the hypothalamus and in the infundibulum. The positive material was localized in the perikarya and in adjacent cytoplasmic processes. Aromatase-containing cells were a specific marker for the sexually dimorphic preoptic nucleus. Treatment with testosterone produced a 6-fold increase in the aromatase activity of the preoptic area and a 4-fold increase in the number of immunoreactive cells in the medial preoptic nucleus. Thus, the increase in aromatase activity observed after testosterone administration is caused by a change in enzyme concentration. [less ▲]

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See detailImmunocytochemical localization of ionotropic glutamate receptors subunits in the adult quail forebrain
Cornil, Charlotte ULg; Foidart, Agnès ULg; Minet, Arlette ULg et al

in Journal of Comparative Neurology (The) (2000), 428

The excitatory amino acid glutamate is implicated in the central control of many neuroendocrine and behavioral processes. The ionotropic glutamate receptors are usually divided into the N-methyl-D ... [more ▼]

The excitatory amino acid glutamate is implicated in the central control of many neuroendocrine and behavioral processes. The ionotropic glutamate receptors are usually divided into the N-methyl-D-aspartate (NMDA) and non-NMDA (kainate and AMPA) subtypes. Subunits of these receptors have been cloned in a few mammalian species. Information available in birds is more limited. In quail, we recently demonstrated that glutamate agonists (kainate, AMPA, and NMDA) rapidly (within minutes) and reversibly decrease in vitro aromatase activity like several other manipulations affecting intracellular HCa21 pools. Aromatase catalyzes the conversion of androgens into estrogens which is a limiting step in the control by testosterone of many behavioral and physiologic processes. Therefore, glutamate could control estrogen production in the brain, but the anatomic substrate supporting this effect is poorly understood. In quail, aromatase is mainly localized in the preoptic-hypothalamic-limbic system. We visualized here the distribution of the major ionotropic glutamate receptors in quail by immunocytochemical methods by using commercial primary antibodies raised against rat glutamate receptor 1 and receptors 2-3 (GluR1, GluR2/3: AMPA subtype, Chemicon, CA), rat glutamate receptors 5-7 (GluR5-7: kainate subtype, Pharmingen, CA), and rat NMDA receptors (NMDAR1, Pharmingen, CA). Dense and specific signals were obtained with all antibodies. The four types of receptors are broadly distributed in the brain, and, in particular, immunoreactive cells are identified within the major aromatase cell groups located in the medial preoptic nucleus, ventromedial hypothalamus, nucleus striae terminalis, and nucleus taeniae. Dense specific populations of glutamate receptor immunoreactive cells are also present with a receptor subtype-specific distribution in broad areas of the telencephalon. The distribution of glutamate receptors, therefore, is consistent with the idea that these receptors could be located at the surface of aromatase-containing cells and mediate the rapid regulation of aromatase activity in a direct manner. [less ▲]

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See detailImmunocytochemical localization of vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the bovine ovary
Hulshof, S. C. J.; Dijkstra, G.; Van der Beek, E. M. et al

in Biology of Reproduction (1994), 50(3), 553-560

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty ... [more ▼]

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty, and from adult cows at three stages of the estrous cycle. The appearance of VIP and NPY immunoreactivity of 4.5-6 mo of gestation coincided with the onset of follicular development. In contrast to NPY, VIP was first found in the cortex. Both VIP and NPY immunoreactivity increased with age. From 9 mo of gestation onwards, VIP and NPY were found around blood vessels and non-vascular smooth muscle cells, in the stroma near preantral follicles, and in the theca externa of antral follicles. In addition, VIP-positive cells were observed exclusively in the granulosa layer of the preovulatory follicle at the time of the LH surge. The distribution of VIP- and NPY-immunoreactive fibers in the ovary may point to an effect of these neuropeptides on various physiological processes, including follicle development and ovarian blood flow. In addition, the presence of VIP-positive cells in the granulosa layer of the preovulatory follicle is indicative of a role for VIP in ovulation. [less ▲]

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See detailImmunocytochemical study of cell type distribution in the pituitary of Barbus barbus (Teleostei, Cyprindidae)
Toubeau, Gérard; Poilve, A.; Baras, Etienne et al

in General and Comparative Endocrinology (1991), 83(1), 35-47

Antisera to mammalian pituitary and placental hormones have been used to identify and localize the different cell types in the pituitary of the barbel (Barbus barbus, L.). The immunocytochemical labeling ... [more ▼]

Antisera to mammalian pituitary and placental hormones have been used to identify and localize the different cell types in the pituitary of the barbel (Barbus barbus, L.). The immunocytochemical labeling employed the immunoperoxidase technique or the immunogold silver staining procedure. Corticotrophic and prolactin cells, visualized using antisera to human adrenocorticotropic hormone and ovine prolactin (PRL), respectively, occur in the rostral pars distalis (RPD). Antisera against mammalian gonadotropins [ovine folliclestimulating hormone (FSH); bovine luteinizing hormone] or porcine growth hormone selectively cross-react with two different cell populations occupying the major part of the proximal pars distalis (PPD). Thyrotropic cells, stained by an antiserum to whole human thyroidstimulating hormone preabsorbed with porcine FSH, are scattered throughout the PPD and found amongst growth hormone and gonadotrophic cells. The majority of pars intermedia cells are stained with anti-melanophore stimulating hormone whereas the scattered PAS positive cells are revealed by both anti-ovine PRL and anti-bovine placental lactogen (or chorionic somatomammotropin). The latter antiserum also cross-reacts with the PRL cells of the RPD. Our results indicate that the distribution of the different cell types in Barbus barbus is similar to that described in other families of teleosts. This report is also the first demonstration of antigenic similarity between mammalian placental lactogen and fish prolactin. [less ▲]

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See detailImmunocytochemical study of subcellular distribution of bovine leukaemia virus regulatory Tax protein
Russev, Russi; Shikova, E.; Portetelle, Daniel ULg et al

Poster (2002, October)

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See detailImmunocytochemical study of subcellular distribution of bovine leukaemia virus regulatory Tax protein.
Russev, R.; Shikova, E.; Portetelle, Daniel ULg et al

in Annals of Oncology (2002), 13(2), 14

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See detailImmunocytochemical techniques reveal new apects in trypanosomatids which harbour endosymbionts
Motta, M C M; De Souza, W; Thiry, Marc ULg

in Memorias do Instituto Oswaldo Cruz (1998), 93

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See detailImmunocytochemistry of lipids : chemical fixatives have dramatic effects on the preservation of tissue lipids
Maneta-Peyret, Lilly; Compère, Philippe ULg; Moreau, Patrick et al

in Histochemical Journal (The) (1999), 31

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See detailImmunocytochemistry of the golden hamster pituitary during chronic administration of diethylstilbestrol: a quantitative analysis using laser scanning microscopy
Van Cauwenberge, A.; Nonclercq, D.; Laurent, G. et al

in Histochemistry & Cell Biology (2001), 115

The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to ... [more ▼]

The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to diethylstilbestrol (DES). Cell proliferation in the hypophysis was monitored by the immunohistochemical demonstration of S-phase cells after pulse labeling with 5-bromo-2'-deoxyuridine. Cell proliferation analysis was combined with the identification of different cell populations by immunostaining with antisera raised against hypophyseal hormones. Sections processed for double-label immunofluorescence were examined by confocal microscopy. In the adenohypophysis, the relative surface occupied by gonadotrophs and thyrotrophs decreased rapidly during the first months of treatment while corticotroph and somatotroph populations remained unaffected. Accordingly, the incidence of S-phase cells in these four cell populations was lower than or similar to control values. In contrast, lactotrophs increased gradually during the first month of exposure to DES to reach a maximum value at 2-4 months. At the beginning, this increase was primarily due to hyperplasia but later on it also involved cellular hypertrophy. Somatomammotrophs did not seem to be involved in this model. In the pars intermedia, the labeling index of melanotrophs rose rapidly to reach values 5-6 times higher than controls. After 4 months, neoplasms originating from the pars intermedia were seen invading both the neuro- and the adenohypophysis. At the end of treatment, the pituitary was markedly enlarged resulting from the development of an adenoma of the pars intermedia [less ▲]

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See detailImmunocytochimie et diagnostic des leucémies aigües: mise au point d'une technique utilisant le complexe phosphatase alcaline-anticorps anti-phosphatase alcaline.
Hayette, Marie-Pierre ULg

Master of advanced studies dissertation (1990)

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure ... [more ▼]

Le diagnostic des leucémies aigues (LA) bénéficie à l'heure actuelle de différents outils complémentaires. Même si la classification FAB (Franco-Américaine-Britannique) reste la référence, il n'en demeure pas moins vrai que l'introduction des anticorps (Ac) monoclonaux a ouvert des perspectives nouvelles en associant des profils immunologiques aux différents types de leucémies aiguës. Des techniques immunocytochimiques utilisant différents enzymes, phosphatases alcaline et peroxydase, associés à des systèmes d'amplification, complexes phosphatase alcaline anticorps anti-phosphatase alcaline (APAAP) ou peroxydase anticorps anti-peroxydase (PAP) ou avidine-biotine peroxydase (ABC), permettent de travailler directement sur frottis sanguins ou médullaires. Ce travail propose une mise au point de la technique à l'APAAP sur lame, afin de répondre au problème posé par les moelles pauvres en blastes dont l'étude est difficile à réaliser par immunofluorescence en cytométrie de flux. [less ▲]

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See detailImmunodetection of bovine chorionic somato-mammotrophin (bCS)
Verstegen, J.; Fellmann, D.; Beckers, Jean-François ULg

in Acta Endocrinologica (1985), 109

Bovine chorionic somatomammotrophin (bCS) was detected in the bovine placenta by using an immunoenzymatic procedure. This hormone appears mainly located in teh binucleate cells of the foetal cotyledons ... [more ▼]

Bovine chorionic somatomammotrophin (bCS) was detected in the bovine placenta by using an immunoenzymatic procedure. This hormone appears mainly located in teh binucleate cells of the foetal cotyledons and to a lesser extent in some localized superficial areas of the foetal binucleate cells migrating into the maternal caruncles. [less ▲]

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See detailImmunodetection of RNA on ultra-thin sections incubated with polyadenylate nucleotidyl transferase.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1993), 41(5), 657-65

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and ... [more ▼]

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and biotinylated ATP. The labeled nucleotides bound to RNA at the surface of the ultra-thin sections were than visualized by an indirect immunogold labeling technique. The resulting labeling pattern was dependent on the presence of divalent cations in the PnT medium. The method revealed with great precision the specific RNA-containing structures within Ehrlich tumor cells. The method is applicable to Epon sections. However, the labeling intensity varies according to the fixation used. Best results were obtained on acetylated cell sections. The method can be combined with EDTA regressive staining. The in situ PnT method provides a very useful tool for pinpointing the precise location of RNA within biological material at the ultrastructural level. [less ▲]

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