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See detailImmuno PCR : a new tool for the ultra-sensitive detection of biomolecules
Zorzi, Willy ULg; Elmoualij, Benaïssa ULg

Conference (2008, December 11)

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See detailImmuno-inflammatory mechanisms in refractory asthma
Manise, Maïté ULg

Doctoral thesis (2012)

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See detailThe Immuno-Inflammatory Reaction in Crohn's Disease and Ulcerative Colitis: Characterisation, Genetics and Clinical Application. Focus on Tnf Alpha
Louis, Edouard ULg

in Acta Gastro-Enterologica Belgica (2001), 64(1), 1-5

Inflammatory bowel diseases are multifactorial polygenic diseases. The author has worked on the characterisation of the mucosal immuno-inflammatory reaction, on genetic predisposition and on potential ... [more ▼]

Inflammatory bowel diseases are multifactorial polygenic diseases. The author has worked on the characterisation of the mucosal immuno-inflammatory reaction, on genetic predisposition and on potential clinical application of blood immuno-inflammatory markers in these diseases. This paper summarizes some aspects of this work, focusing on TNF. Following points are developed: production of TNF by inflamed mucosa, genetic control of TNF production, TNF gene polymorphim in inflammatory bowel disease, and evaluation of serum TNF as a marker of disease activity or evolutivity. [less ▲]

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See detailAn immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyond
Ruelle, Virginie ULg; Falisse-Poirier, Nandini; Elmoualij, Benaïssa ULg et al

in Journal of Proteome Research (2007), 6(6), 2168-2175

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies ... [more ▼]

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry ( i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model. [less ▲]

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See detailImmuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
Gofflot, Stéphanie ULg; Elmoualij, Benaïssa ULg; Zorzi, Danièle ULg et al

in Journal of Immunoassay & Immunochemistry (2004), 25(3), 241-258

Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR ... [more ▼]

Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage. [less ▲]

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See detailImmunoassay using a biofunctionnalized alumina-coated capacitive biosensor: towards a microfluidic detection of the H5N1 Influenza virus
Overstraten-Schlogel, Nancy; Depuis, Pascal; Lefévre, Olivia et al

Conference (2011, December 02)

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See detailImmunobiology of reduced intensity conditioning in hematopoietic stem cell transplantation
Baron, Frédéric ULg; Sandmaier, Brenda

in Hematology education : the education program for the annual congress of the European Hematology Association (2009)

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See detailIMMUNOCAP© ISAC: INTEREST IN ALLERGY DIAGNOSIS
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Poster (2009, November 19)

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See detailImmunoCAP© ISAC: interest in allergy diagnosis
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Conference (2009, April 28)

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See detailImmunocastration of farm animals
Portetelle, Daniel ULg; Haezebroeck, Valérie; Mortiaux, Frédéric et al

in Bases (2000), 4

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See detailImmunocastration of farm animals.
Mestdagt, M.; Portetelle, Daniel ULg; Bertozzi, C. et al

in Biotechnology in animal husbandry (2001)

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See detailThe immunochemical determinations of serum lipase in acute pancreatitis: further results.
Adam, A.; Boulanger, J.; Chapelle, Jean-Paul ULg et al

in Clinical Chemistry (1986), 32(10), 1987

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See detailImmunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Rusu, D.; Loret, S.; Peulen, Olivier ULg et al

in BMC Cell Biology (2005), 6

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs ... [more ▼]

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract. Results: Cultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium. Conclusion: The present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations. [less ▲]

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See detailImmunocytochemical demonstration of hormones in cells granules of several human pituitary adenomas
Beckers, Albert ULg; Stevenaert, Achille ULg; Courtoy, R. et al

in Histochemical Journal (The) (1986)

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See detailImmunocytochemical demontration of hormones in cell granules of several human pituitary adenomas
Beckers, Albert ULg; Courtoy, R.; Stevenaert, Achille ULg et al

in Histochemical Journal (The) (1986), 18(1), 55

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See detailImmunocytochemical detection of DNA and RNA in endosymbiont-bearing trypanosomatids.
Motta, Maria Cristina M; de Souza, Wanderley; Thiry, Marc ULg

in FEMS Microbiology Letters (2003), 221(1), 17-23

Research about the kinetoplast of trypanosomatids has yielded valuable information about the organization of extranuclear structure. However, the ultrastructural localization of nucleic acids within these ... [more ▼]

Research about the kinetoplast of trypanosomatids has yielded valuable information about the organization of extranuclear structure. However, the ultrastructural localization of nucleic acids within these protozoa remains uncertain. We have applied cytochemical and immunocytochemical approaches to precisely identify DNA and RNA in lower endosymbiont-bearing trypanosomatids. Using the Terminal deoxynucleotidyl Transferase (TdT) immunogold technique, we showed that nuclear DNA is seen associated with the nuclear envelope during the trypanosomatid cell cycle. By combining the TdT technique with the acetylation method, which improves the contrast between structures containing fibrils and granules, we have demonstrated that the nucleolus of endosymbiont-bearing trypanosomatids is composed of two constituents: a granular component and a DNA-positive fibrillar zone. Moreover, we revealed that DNA of endosymbiotic bacteria consisted of electron-dense filaments which are usually in close contact with the prokaryote envelope. Using a Lowicryl post-embedding immunogold labeling procedure with anti-RNA antibodies, we showed the presence of RNA not only over the cytoplasm, the interchromatin spaces and the nucleolus, but also over the kinetoplast and virus-like particles present in Crithidia desouzai. [less ▲]

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See detailImmunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
Bastings, E.; Beckers, Albert ULg; Reznik, Michel ULg et al

in Biology of Reproduction (1991), 45(5), 788-96

In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based ... [more ▼]

In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LH in secretory granules of gonadotrophs using highly specific antisera. This morphological study included light microscopy (PAP) and electron microscopy (immunogold single and double labeling) procedures. Histologically, approximatively 11.5% of cells were positive for LH, whereas only 5.4% of cells were positive for FSH. With the electron microscope, single labeling allowed identification of morphologically distinct LH-containing cells and FSH-containing cells. Double immunostaining confirmed that no cells contained both hormones. The finding that FSH and LH are produced in separate pituitary cells is in agreement with recent studies that have suggested a specific role and regulatory process for gonadotropins in the bovine species. [less ▲]

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