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See detailIdentification of heterologous multicopy suppressors of the yeast msb3msb4 double mutation
Delroisse, Jean-Marc; Breban, Martine; Burny, Arsène et al

in Yeast (2003), 20(S291),

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See detailIdentification of heterologous multicopy suppressors of the yeast msb3msb4 double mutation
Delroisse, Jean-Marc; Breban, Martine; Burny, Arsène et al

in Archives Internationales de Physiologie et de Biochimie (2003), 184

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See detailIdentification of heterologous multicopy suppressors of the yeast msb3msb4 double mutation
Delroisse, Jean-Marc; Breban, Martine; Burny, Arsène et al

Poster (2003)

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See detailIdentification of heterologous multicopy suppressors of the yeast msb3msb4 double mutation.
Delroisse, Jean-Marc; Breban, Martine; Burny, Arsène et al

in Archives Internationales de Physiologie et de Biochimie (2003), 184

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See detailIdentification of HTF (HER2 transcription factor) as an AP-2 (activator protein-2) transcription factor and contribution of the HTF binding site to ERBB2 gene overexpression
Vernimmen, Douglas; Begon, Dominique ULg; Salvador, Christophe et al

in Biochemical Journal (2003), 370(Pt 1), 323-329

The ERBB2 gene is overexpressed in 30% of human breast cancers and this is correlated with poor prognosis. Overexpression of the ERBB2 gene is due to increased transcription and gene amplification. Our ... [more ▼]

The ERBB2 gene is overexpressed in 30% of human breast cancers and this is correlated with poor prognosis. Overexpression of the ERBB2 gene is due to increased transcription and gene amplification. Our previous studies have identified a new cis element in the ERBB2 promoter which is involved in the gene's overexpression. This cis element, located 501 bp upstream from the main ERBB2 transcription initiation site, binds a transcription factor called HTF (HER2 transcription factor). We report here the identification of HTF as an AP-2 (activator protein-2) transcription factor. The new cis element is bound by AP-2 with high affinity, compared with a previously described AP-2 binding site located 284 bp downstream. Co-transfection of an AP-2alpha expression vector with a reporter vector containing the newly identified AP-2 binding site in front of a minimal ERBB2 promoter induced a dose-dependent increase in transcriptional activity. We examined the contribution of the new AP-2 binding site to ERBB2 overexpression. For this purpose we abolished the new and/or the previously described AP-2 binding sequence by site-directed mutagenesis. The results show that the two functional AP-2 sites in the first 700 bp of the ERBB2 promoter co-operate to achieve maximal transcriptional activity. [less ▲]

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See detailIdentification of key predictors for osteoporosis through use of different analytical techniques
Sen, S. S.; Geling, O.; Messina, O. D. et al

in Osteoporosis International (2002, November), 13(Suppl.3), 27

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See detailIdentification Of Key Residues For Interaction Of Vasoactive Intestinal Peptide With Human Vpac(1) And Vpac(2) Receptors And Development Of A Highly Selective Vpac(1) Receptor Agonist - Alanine Scanning And Molecular Modeling Of The Peptide
Nicole, P.; Lins, Laurence ULg; Rouyer-Fessard, C. et al

in Journal of Biological Chemistry (2000), 275(31), 24003-12

The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC(1) and VPAC(2). Solid-phase syntheses of VIP analogs in which each amino acid has been changed to alanine (Ala scan ... [more ▼]

The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC(1) and VPAC(2). Solid-phase syntheses of VIP analogs in which each amino acid has been changed to alanine (Ala scan) or glycine was achieved and each analog was tested for: (i) three-dimensional structure by ab initio molecular modeling; (ii) ability to inhibit (125)I-VIP binding (K(i)) and to stimulate adenylyl cyclase activity (EC(50)) in membranes from cell clones stably expressing human recombinant VPAC(1) or VPAC(2) receptor. The data show that substituting residues at 14 positions out of 28 in VIP resulted in a >10-fold increase of K(i) or EC(50) at the VPAC(1) receptor. Modeling of the three-dimensional structure of native VIP (central alpha-helice from Val(5) to Asn(24) with random coiled N and C terminus) and analogs shows that substitutions of His(1), Val(5), Arg(14), Lys(15), Lys(21), Leu(23), and Ile(26) decreased biological activity without altering the predicted structure, supporting that those residues directly interact with VPAC(1) receptor. The interaction of the analogs with human VPAC(2) receptor is similar to that observed with VPAC(1) receptor, with three remarkable exceptions: substitution of Thr(11) and Asn(28) by alanine increased K(i) for binding to VPAC(2) receptor; substitution of Tyr(22) by alanine increased EC(50) for stimulating adenylyl cyclase activity through interaction with the VPAC(2) receptor. By combining 3 mutations at positions 11, 22, and 28, we developed the [Ala(11,22,28)]VIP analog which constitutes the first highly selective (>1,000-fold) human VPAC(1) receptor agonist derived from VIP ever described. [less ▲]

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See detailIdentification of large selective sweeps associated with major genes in cattle.
Druet, Tom ULg; Perez-Pardal, L.; Charlier, Carole ULg et al

in Animal Genetics (2013), 44(6), 758-62

Selection for new favorable variants can lead to selective sweeps. However, such sweeps might be rare in the evolution of different species for which polygenic adaptation or selection on standing ... [more ▼]

Selection for new favorable variants can lead to selective sweeps. However, such sweeps might be rare in the evolution of different species for which polygenic adaptation or selection on standing variation might be more common. Still, strong selective sweeps have been described in domestic species such as chicken lines or dog breeds. The goal of our study was to use a panel of individuals from 12 different cattle breeds genotyped at high density (800K SNPs) to perform a whole-genome scan for selective sweeps defined as unexpectedly long stretches of reduced heterozygosity. To that end, we developed a hidden Markov model in which one of the hidden states corresponds to regions of reduced heterozygosity. Some unexpectedly long regions were identified. Among those, six contained genes known to affect traits with simple genetic architecture such as coat color or horn development. However, there was little evidence for sweeps associated with genes underlying production traits. [less ▲]

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See detailIdentification of lectins as virus competitors in aphid vectors: from fundamental to applied approaches
Bosquée, Emilie ULg; Chen, Ju Lian; Yong, Liu et al

Poster (2014, August)

Aphids remain difficult virus vectors to be controlled in many crops. Within this context of biological control against aphids and investigation on virus transmission (both non persistent Potato virus Y ... [more ▼]

Aphids remain difficult virus vectors to be controlled in many crops. Within this context of biological control against aphids and investigation on virus transmission (both non persistent Potato virus Y (PVY) and Cucumber Mosaic Virus (CMV) and persistent Barley Yellow Dwarf Virus, the use of lectins was developed. Indeed, the effects of lectin binding to glycoprotein aphid receptors on virus transmission were tested in laboratory via the use of artificial diets using different virus models. Differential effects on virus transmission efficiency were determined. By example, a strong interference between GNA and PVY was observed. In consequence, the lectin was considered as promising proteins and could be potential competitors to virus. For further research, a fast protein liquid chromatography was performed to identify aphid proteins related to binding to efficient lectin competitors. Moreover, to detect involved proteins in aphids, two-dimensional gel electrophoresis approach coupled to mass spectrometry was developped to identify the glycosylated proteins determining virus transmission efficiency. Use of lectins for both fundamental and applied approaches were discussed to investigate aphid – virus interactions [less ▲]

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See detailIdentification of Linear and Nonlinear Systems using Signal Processing Techniques
Kerschen, Gaëtan ULg; Poncelet, Fabien ULg; Golinval, Jean-Claude ULg et al

in 26th Benelux Meeting on Systems and Control, Lommel, 2007 (2007)

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See detailIdentification of lines in the spectra of B stars
Swings, Polydore ULg; Nicolet, M.

in Astrophysical Journal (1934), 80(3), 190-199

Identifications of lines in the spectra of B stars are given. About fifty lines for which no previous identifications had been made are interpreted.

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See detailIdentification of material parameters to predict Single Point Incremental Forming forces
Bouffioux, Chantal ULg; Eyckens, P.; Henrard, Christophe et al

in International Journal of Material Forming (2008)

The purpose of this article is to develop an inverse method for adjusting the material parameters for single point incremental forming (SPIF). The main idea consists in FEM simulations of simple tests ... [more ▼]

The purpose of this article is to develop an inverse method for adjusting the material parameters for single point incremental forming (SPIF). The main idea consists in FEM simulations of simple tests involving the SPIF specificities (the “line test”) performed on the machine used for the process itself. This approach decreases the equipment cost. It has the advantage that the material parameters are fitted for heterogeneous stress and strain fields close to the ones occurring during the actual process. A first set of material parameters, adjusted for the aluminum alloy AA3103 with classical tests (tensile and cyclic shear tests), is compared with parameters adjusted by the line test. It is shown that the chosen tests and the strain state level have an important impact on the adjusted material data and on the accuracy of the tool force prediction reached during the SPIF process. [less ▲]

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See detailIdentification of material parameters using bi-axial machine
Flores, Paulo; de Montleau, P.; Mathonet, V. et al

in Stören, Sigurd (Ed.) Proceedings of the 7th ESAFORM conference on Materials Forming (2004)

Experimental testing equipment is built in order to identify material parameters of complex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able to perform plane ... [more ▼]

Experimental testing equipment is built in order to identify material parameters of complex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able to perform plane strain and simple shear tests separately or simultaneously and a Miyauchi simple shear test device; an optical extensometer is used to identify the strain field. The article focus on the validation of the results of this new equipment by comparing with results obtained by standard machines and/or by using FEM simulations of already identified materials. The first results show that the bi-axial machine performs the plane strain test optimally and some modifications are proposed in order to improve the results for the simple shear test. Miyauchi device shows a good performance and the experimental results are compared with a FEM simulation. [less ▲]

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See detailIdentification of mechanical systems with local nonlinearities through discrete-time Volterra series and Kautz functions
Shiki, Sidney Bruce; Noël, Jean-Philippe ULg; Kerschen, Gaëtan ULg et al

in Proceedings of the 11th International Conference on Recent Advances in Structural Dynamics (2013, July)

Mathematical modeling of mechanical structures is an important research area in structural dynamics. One of the goals of this area is to obtain a model that accurately predicts the dynamics of the system ... [more ▼]

Mathematical modeling of mechanical structures is an important research area in structural dynamics. One of the goals of this area is to obtain a model that accurately predicts the dynamics of the system. However, the nonlinear eff ects caused by large displacements and boundary conditions like gap, backlash or joint are not as well understood as the linear counterpart. This paper identifies a non-parametric discrete-time Volterra model of a benchmark nonlinear structure consisting of a cantilever beam connected to a thin beam at its free end. Time-domain data experimentally measured are used to identify the Volterra kernels, which are expanded with orthogonal Kautz functions to facilitate the identification process. The nonlinear parameters are then estimated through a model updating process involving optimization of the residue between the numerical and experimental kernels. The advantages and drawbacks of the Volterra series for modeling the behavior of nonlinear structures are finally indicated with suggestions to overcome the disadvantages found during the tests. [less ▲]

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See detailIdentification of mechanisms involved in the acute airway toxicity induced by parathion.
Segura, P.; Chavez, J.; Montano, L. M. et al

in Naunyn-Schmiedeberg's Archives of Pharmacology (1999), 360(6), 699-710

Organophosphates are still widely used worldwide and cause thousands of intoxications every year. In this work we investigated the mechanisms of parathion (Pth) airway toxicity, using biochemical and ... [more ▼]

Organophosphates are still widely used worldwide and cause thousands of intoxications every year. In this work we investigated the mechanisms of parathion (Pth) airway toxicity, using biochemical and functional approaches. A plethysmographic technique for unrestrained guinea pigs was used to analyze Pth-induced modifications of airway mechanics and responsiveness to acetylcholine (ACh: 0.1-3.2 mg/ml, 2-min inhalation each dose). The isolated perfused rabbit lung preparation was used to study the acute effects of Pth on airway responsiveness to ACh (10(-8)-10(-3) M), histamine (10(-8)-10(-3) M) and substance P (10(-10)-10(-6) M), pulmonary acetylcholinesterase inhibition and cytochrome P450 (P450) activity, and their modifications with previous administration of Pth (1 mg/kg s.c. daily, 7 days). We found that: (1) In guinea pigs Pth (3.2-17 mg/kg i.p.) produced a dose-dependent increase in a lung resistance index (iRL), which was greatly reverted (approximately 50%) by salbutamol (2 mg/ml, 2-min inhalation, or 10 microg/kg i.p.). This salbutamol effect was transient (5-10 min), suggesting that this bronchodilator triggered additional obstructive mechanisms. (2) Pth increased the water content in lung parenchyma samples, but not in trachea or bronchi, and augmented the respiratory secretions measured through monosaccharide content in bronchoalveolar lavage. (3) The increase in iRL was greater in female animals, probably due to a higher P450 basal activity, and completely blocked by pharmacological inhibition of P450 with piperonyl butoxide (500 mg/kg i.p.). (4) In male guinea pigs a subclinical dose of Pth (10 mg/kg i.p.) induced airway hyperresponsiveness to ACh. In isolated perfused rabbit lung Pth (10(-6) M) produced airway hyperresponsiveness to ACh and histamine, the latter prevented by atropine (10(-5) M). (5) Repetitive exposure to subclinical doses (1 mg/kg s.c.) of Pth during 1 week caused approximately 80% inhibition of P450 activity in rabbits, which was not enough, however, to prevent the functional manifestation of Pth toxicity in the airways. [less ▲]

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See detailIdentification of Metabolic Pathways Expressed by Pichia anomala KH6 in the Presence of the Pathogen Botrytis cinerea on Apple: New Possible Targets for Biocontrol Improvement
Kwasiborski, Anthony; Bajji, Mohammed; Renaut, J. et al

in PLoS ONE (2014), 9(3),

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See detailIdentification of Metabolites in a Human Plasma Standard Reference Material by Comprehensive two Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry
Eppe, Gauthier ULg; Mc Gaw, Elizabeth; Dodder, Nathan et al

in 57th ASMS Conference on Mass Spectrometry and Allied Topics (2009)

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See detailIDENTIFICATION OF METABOLITES IN A HUMAN PLASMA STANDARD REFERENCE MATERIAL BY MULTIPLE GC/MS APPROACHES
Eppe, Gauthier ULg; Benner, Bruce; White, Edward et al

Poster (2010, January)

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