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See detailFundamental property and functional aptitude relationships in emulsion and foam applications of amphiphilic compounds
Razafindralambo, Hary ULg

Conference (2013, April 11)

Amphiphilic compounds are issued of a combination of two opposite entities, covalently assemblied within a single structure. By the diversity of the origin, class, number of monomer and substitute, size ... [more ▼]

Amphiphilic compounds are issued of a combination of two opposite entities, covalently assemblied within a single structure. By the diversity of the origin, class, number of monomer and substitute, size, linker or spacer, stereochemistry of the molecular entities, their chemical structure may be very different but also close each other. However, they may fulfill the same role in reducing surface and interfacial free energy in dynamic or static ways, ensuring especially the formation of dispersed systems like emulsion and foam. If the relationships between the structural variable and fundamental properties are strictly chemical residue dependent, those between fundamental and functional aptitudes are not necessarily. In this case, general and universal rules may exist, whatever the kind of amphiphilic compounds. In order to achieve a rational design for any expected functionality, we attempt to find out some predictive and conception rules from a lot of screening data of fundamental and functional properties. In this communication, some correlations between fundamental data related to surface and interfacial properties, measured by molecule adsorption or spreading under dynamic or static mode, in compression or dilation of adsorbed or spread film, and emulsifying and foaming performances of various bio-based surfactants will be presented. [less ▲]

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See detailA fundamental study of the inception of motion of coarse sediments
Duma, Diana ULg; Erpicum, Sébastien ULg; Archambeau, Pierre ULg et al

in Proceedings of 2013 IAHR World Congress (2013, September)

This research aims to provide new knowledge about the inception of motion in rivers with coarse sediments and, possibly, macro-roughness flow regime, for which, so far, civil and environmental engineers ... [more ▼]

This research aims to provide new knowledge about the inception of motion in rivers with coarse sediments and, possibly, macro-roughness flow regime, for which, so far, civil and environmental engineers face much uncertainty in the determination of threshold hydraulic conditions for sediment transport. The threshold conditions have to be clearly defined, at the theoretical level, and well identified, in experimental tests. Therefore, a specific program of laboratory experiments has been designed. It relies on two complementary laboratory flumes of, respectively, 6m and 20m in length (discharge up to 100 l/s, slope between 0 and 4%). Three different thresholds have been defined to distinguish between the following four states: no motion, vibration of stone, local motion, and large scale motion. Based on experimental tests conducted so far in the smaller flume, velocity profile and turbulence intensity measured in quasi-uniform and non-uniform flow conditions have been correlated with the inception of motion. Flow velocity was measured using UVP transducers. In this paper, the results of a first phase of the study are presented, with the aim of validating the experimental setup, procedure and instrumentation. They focus particularly on the feasibility of collecting the relevant flow variables (shear velocity, turbulence) from a limited number of pointwise velocity measurements. [less ▲]

Detailed reference viewed: 66 (11 ULg)
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See detailFundamentals and biotechnological aspects of cold-adapted enzymes
Gerday, Charles ULg; Collins, Tony; D'Amico, Salvino et al

Conference (2006)

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See detailFundamentals of Cold-Adapted Enzymes
D'Amico, Salvino ULg

Scientific conference (2007)

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See detailFundamentals of Cold-Adapted Enzymes
Collins, T.; Roulling, Frédéric ULg; Piette, Florence ULg et al

in Margesin, R.; Schinner, F.; Gerday, Charles (Eds.) et al Psychrophiles: from Biodiversity to Biotechnology (2008)

Detailed reference viewed: 37 (15 ULg)
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See detailFundamentals of finite elements
Debongnie, Jean-François ULg

Book published by Les Editions de l'Université de Liège (2003)

This book presents the text of an introductory course on finite element methods. As written by a member of the Aerospace Laboratory of the University of Liège, it reflects the general philosophy of this ... [more ▼]

This book presents the text of an introductory course on finite element methods. As written by a member of the Aerospace Laboratory of the University of Liège, it reflects the general philosophy of this pioneering research team. [less ▲]

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See detailFundamentals of transformed skeletal muscle used for cardiac assistance. An overview
Radermacker, M. A.; Chachques, J. C.; Sluse, Francis ULg et al

in Research in Surgery (1995), 7

Detailed reference viewed: 15 (6 ULg)
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See detailFundic Argyrophil Cell Hyperplasia in Atrophic Gastritis: A Search for a Sensitive Diagnostic Method
Belaiche, Jacques ULg; Delwaide, Jean ULg; Vivario, M. et al

in Acta Gastro-Enterologica Belgica (1993), 56(1, Jan-Feb), 11-7

Hypergastrinemia induces argyrophil cell hyperplasia in oxyntic mucosa (FACH) in patients with non-antral atrophic gastritis, with or without pernicious anemia. This proliferation favours the development ... [more ▼]

Hypergastrinemia induces argyrophil cell hyperplasia in oxyntic mucosa (FACH) in patients with non-antral atrophic gastritis, with or without pernicious anemia. This proliferation favours the development of carcinoid tumours. In order to determine the most usual appropriate method to document FACH, we have studied 29 consecutive fundic biopsies from 26 patients with fundic chronic gastritis. The study encompassed gastrinemia levels, standard histology permitting the classification of chronic gastritis, demonstration of FACH by Grimelius stain, immunohistochemical studies using NSE, chromogranin A and by electron microscopy. The FACH was classified for each stain as slight, moderate or severe. The study displayed a relationship between the grade of gastritis and the density of argyrophil endocrine cells in oxyntic mucosa assessed by Grimelius stain (p < 0.0001) and chromogranin A (P < 0.01). There was also a relationship between the serum gastrin level and the density of argyrophil endocrine cells detected by these two stains (p < 0.001). A highly significant correlation was observed between Grimelius stain and chromogranin A (p < 0.0001). On the other hand, no significant correlation was noted with either NSE or electron microscopy. We conclude that Grimelius stain and immunohistochemical studies against chromogranin A were the best methods for the demonstration of FACH in atrophic gastritis. One of these two techniques is sufficient in current practice for defining the patients at risk for subsequent surveillance. [less ▲]

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See detailA fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Gofflot, Sebastien et al

in Applied Microbiology and Biotechnology (2015)

Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state related physiological mechanisms. This work is based on the comparative analysis of classical ... [more ▼]

Fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state related physiological mechanisms. This work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a Gla::GFP fusion protein by Aspergillus oryzae. The biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. Since the production of the target protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, the biofilm mode of culture is expected to enhance the global productivity. Although production of the target protein was enhanced by using the biofilm mode of culture, we also found that fusion protein production is also significant when the submerged mode of culture is used. This result is related to high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights the preservation of fusion protein integrity produced in biofilm conditions. Two fungal biofilm reactor designs were then investigated further, i.e. with full immersion of the packing or with medium recirculation on the packing, and the scale-up potentialities were evaluated. In this context, it has been shown that full immersion of the metal packing in the liquid medium during cultivation allows for a uniform colonization of the packing by the fungal biomass and leads to a better quality of the fusion protein. [less ▲]

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See detailFungal biofilm reactor improves the productivity of hydrophobin HFBII
Khalesi, Mohammadreza; Zune, Quentin ULg; Telek, Samuel ULg et al

in Biochemical Engineering Journal (in press)

Production and purification of hydrophobin HFBII has recently been the subject of intensive research, but the yield of production needs to be further improved for a generic use of this molecule at ... [more ▼]

Production and purification of hydrophobin HFBII has recently been the subject of intensive research, but the yield of production needs to be further improved for a generic use of this molecule at industrial scale. In a first step, the influence of different carbon sources on the growth of Trichoderma reesei and the production of HFBII was investigated. The optimum productivity was obtained by using 40 g/L lactose. Carbon starvation and excretion of extracellular enzyme were determined as two main conditions for the production of HFBII. In the second phase, and according to the physiological mechanisms observed during the screening phase, a bioreactor set up has been designed and two modes of cultures have been investigated, i.e. the classical submerged fermentation and a fungal biofilm reactor. In this last set-up, the broth is continuously recirculated on a metal packing exhibiting a high specific surface. In this case, the fungal biomass was mainly attached to the metal packing, leading to a simplification of downstream processing scheme. More importantly, the HFBII concentration increased up to 48.6 ± 6.2 mg/L which was 1.8 times higher in this reactor configuration and faster than the submerged culture. X-ray tomography analysis shows that the biofilm overgrowth occurs when successive cultures are performed on the same packing. However, this phenomenon has no significant influence on the yield of HFBII, suggesting that this process could be operated in continuous mode. Protein hydrolysis during stationary phase was observed by MALDI-TOF analysis according to the removal of the last amino acid from the structure of HFBII after 48 h from the beginning of fermentation in biofilm reactor. Hopefully this modification does not lead to alternation of the main physicochemical properties of HFBII. [less ▲]

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See detailFungal chitin-glucan scaffold for managing diabetic xerosis of the feet in menopausal women.
Quatresooz, Pascale ULg; Franchimont, Claudine ULg; Szepetiuk, Grégory ULg et al

in Expert Opinion on Pharmacotherapy (2009), 10(14), 2221-9

BACKGROUND: Corneocyte accumulation (hyperkeratosis, xerosis) commonly occurs in the stratum corneum (SC) of the feet of diabetic patients, as well as menopausal women. OBJECTIVE: To compare the effects ... [more ▼]

BACKGROUND: Corneocyte accumulation (hyperkeratosis, xerosis) commonly occurs in the stratum corneum (SC) of the feet of diabetic patients, as well as menopausal women. OBJECTIVE: To compare the effects of a 2.5% chitin-glucan formulation with its placebo, and commercially available glycerol formulations. METHODS: This two-step controlled double-blind, randomized, intra-individual study was performed in 30 type 1 and 2 diabetic menopausal women suffering from xerosis of the feet. The formulations were applied once daily for 3 weeks. Electrometric assessments were performed on three sites of the feet at entry in the study, at weekly intervals during the treatment phase, and in a 2-week follow-up out of treatment. Positive controls consisted in two commercially available formulations enriched in glycerol. RESULTS: Data revealed an unequivocal benefit provided by the 2.5% chitin-glucan formulation compared with placebo. The electrometric values were significantly higher at each evaluation time during both treatment and follow-up phases. The two glycerol-enriched formulations showed slightly different kinetics of SC moisturization. A steep increase was followed by a plateau level and a rapid decline after stopping the treatments. CONCLUSION: The increased moisturization of the SC of the sole probably improves the desquamation process and reduces xerosis of the soles. [less ▲]

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See detailFungal contamination in barley and Kashin-Beck disease in Tibet
Chasseur, Camille; Suetens, Carl; Nolard, Nicole et al

in Lancet (1997), 350

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See detailFungal gene expression in a forest soil
Kellner, Harald ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2010, August)

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See detailThe fungal hypotheses (chapter 5.1)
Chasseur, Camille; Lognay, Georges ULg; Suetens, Carl et al

in Big Bone Disease : A multidisciplinary approach of Kasing-Beck disease in Tibet Autonomous Region (P.R. China) (2008)

Detailed reference viewed: 64 (7 ULg)
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See detailFungal lectin, XCL, is internalized via clathrin-dependent endocytosis and facilitates uptake of other molecules.
Francis, Frédéric ULg; Marty-Detraves, Claire; Poincloux, Renaud et al

in European Journal of Cell Biology (2003), 82(10), 515-22

The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect ... [more ▼]

The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect-resistant transgenic plants, we investigated the effects of XCL at the cellular level. Immunofluorescence microscopy studies revealed that XCL is rapidly internalized into small endocytic vesicles that further coalesce in the perinuclear region. We show that XCL is endocytosed by the clathrin-dependent pathway, and is delivered to late endosome/lysosome compartments. The internalization of XCL seems to be general since it occurs in different cell types such as insect (SF9) or mammalian (NIH-3T3 and Hela) cell lines. In the presence of XCL, the uptake of GFP and BSA is greatly enhanced, demonstrating that XCL facilitates endocytosis. Thus, XCL could serve as a delivery agent to facilitate the endocytosis of proteins that do not enter the cell alone. [less ▲]

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See detailFungal lipase production by solid-state fermentation
Ramos-Sanchez, L.B.; Julian-Ricardo, M.C.; Cordova, J. et al

Conference (2014, December)

Detailed reference viewed: 17 (2 ULg)
Peer Reviewed
See detailFungal skin infections in organ transplant recipients.
QUATRESOOZ, Pascale ULg

Conference (2003)

Detailed reference viewed: 7 (0 ULg)