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See detailEvaluation of the genetic diversity of honey bees, Apis mellifera L. in the Walloon Region and selection of strains tolerant to the mite Varroa destructor Anderson and Trueman
Leclercq, Gil ULg; Nguyen, Bach Kim ULg; Gengler, Nicolas ULg et al

Poster (2012, February 10)

For more than a decade, high losses of honey bee colonies have been noticed in several countries, including Belgium. Currently often the mite Varroa destructor is considered a main threat for beekeeping ... [more ▼]

For more than a decade, high losses of honey bee colonies have been noticed in several countries, including Belgium. Currently often the mite Varroa destructor is considered a main threat for beekeeping. In view of the inefficiency of the current chemical treatments, one of the solutions is to select honey bees tolerant to this parasite using genomic selection. To reach this objective the genetic diversity of honey bees needs first to be studied using mainly ‘Single Nucleotide Polymorphisms’ (SNP). Records and samples will be collected all over the Walloon Region in order to create an informative phenotypic and genomic data base that will be used for ‘Genome Wide Association Studies’ (GWAS) to detect associations between SNPs and tolerance, and to select bees tolerant to Varroa destructor. [less ▲]

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See detailEvaluation Of The Genetic Variability Of 23 Bovine Microsatellite Markers In Four Belgian Cattle Breeds
Peelman, Lj.; Mortiaux, F.; Van Zeveren, A. et al

in Animal Genetics (1998), 29(3),

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See detailEvaluation of the GERD Impact Scale, an international, validated patient questionnaire, in daily practice. Results of the ALEGRIA study.
Louis, Edouard ULg; Tack, Jacques ULg; Vandenhoven, G. et al

in Acta Gastro-Enterologica Belgica (2009), 72(1), 3-8

BACKGROUND AND STUDY AIMS: Gastroesophageal reflux disease (GERD) is a common chronic disease that is primarily diagnosed based on symptom severity and frequency. This study gathered epidemiological data ... [more ▼]

BACKGROUND AND STUDY AIMS: Gastroesophageal reflux disease (GERD) is a common chronic disease that is primarily diagnosed based on symptom severity and frequency. This study gathered epidemiological data in a population of GERD patients and evaluated the added-value of the GERD Impact Scale (GIS), a novel, validated patient questionnaire, as a tool for initial and long-term patient management. PATIENTS AND METHODS: This observational study recruited patients (296 study centers) with symptomatic GERD and a history of erosive, or reflux, esophagitis. Symptoms were assessed by GIS and physician-subject interview and recorded at baseline (visit 1), at 4-6 weeks (visit 2) and 8-14 weeks (visit 3); also recorded at each visit was the physician's assessment of GERD severity and treatment changes. Analyses were performed on an intent-to-treat basis. RESULTS: Subjects (n = 1919; mean age, 55 years) were 54% female. Lifestyle characteristics included stress (approximately 70% of subjects), mean daily consumption of five cups of caffeine-containing beverages (approximately 70%), alcohol consumption of approximately nine units per week (approximately 50%) and smoking/ex-smoker (41%). Proton pump inhibitors were prescribed in 99% of cases: mainly esomeprazole (82%), with a median dose of 40 mg. Prescribed therapy was changed (mainly dosage levels) between visits in approximately 60% of subjects. The severity of GERD symptoms and GIS scores decreased substantially throughout the study. Mean GIS scores correlated positively with increasing GERD severity and clinical judgment at all visits. Physicians reported that the GIS helped them define the appropriate treatment for the patient and to evaluate the patient's response to treatment in 81% of cases. CONCLUSIONS: This study demonstrates the added-value and usefulness of the patient self-assessment GIS as a management tool for GERD. [less ▲]

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See detailEvaluation of the Greenland ice sheet surface mass balance simulated by a regional climate model forced by some selected IPCC AR5/CMIP5 AOGCMs over the current climate.
Fettweis, Xavier ULg; van den Broeke, Michiel; van de Berg, Willem Jan et al

Conference (2011, April 05)

Abstract. As part of the ICE2SEA project, the regional climate model MAR was forced by the general circulation model ECHAM5 for making future projections of the Greenland Ice Sheet (GrIS) Surface Mass ... [more ▼]

Abstract. As part of the ICE2SEA project, the regional climate model MAR was forced by the general circulation model ECHAM5 for making future projections of the Greenland Ice Sheet (GrIS) Surface Mass Balance (SMB) over 1980-2099 at a resolution of 25km. For the A1B scenario, MAR projects a highly negative (-500 GT/yr) SMB rate at the end of this century and a induced mass loss corresponding to a sea level rise of ~7 cm over 2000-2100. However, the comparison with MAR forced by the ERA-40 reanalysis over 1980-1999 shows that MAR forced by the 20C3M scenario is not able to represent reliably the current SMB due to biases in the general circulation and in the free atmosphere summer temperature modeled by ECHAM5 around the GrIS. These biases induce in MAR an underestimation of the snow accumulation and an overestimation of the surface melt. Therefore, this questions the reliability of these ECHAM5-forced future projections, knowing that i) these biases could be amplified in future and that ii) the MAR outputs are used to force ice sheets models for the ICE2SEA project. That is why, by waiting the outputs from the next generation of GCMs (CMIP5), we investigate the impacts of current climate biases over the future projections and we suggest corrections of ECHAM5 forcing files for having a better agreement with the ERA-40 forced simulation. This is useful for the ice sheet model wanting to use the absolute values of MAR future projections instead of anomalies. [less ▲]

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See detailEvaluation of the group B differential agar for the detection of group B streptococci from vaginal specimens
MELIN, Pierrette ULg; Rodriguez Cuns, Grisel; Lorquet, Sophie et al

in American Society of Microbiology (Ed.) Program and Abstracts of the 104th General Meeting of the American Society for Microbiology (2004, May)

Background Group B streptococci (GBS) are the leading cause of severe perinatal infections. Most current guidelines for the prevention of GBS perinatal disease are based on prenatal screening culture for ... [more ▼]

Background Group B streptococci (GBS) are the leading cause of severe perinatal infections. Most current guidelines for the prevention of GBS perinatal disease are based on prenatal screening culture for vaginal GBS colonisation. Use of selective and differential media could improve the sensitivity of these cultures. Objective To evaluate the GBS-Differential Agar (GBSDA) recently formulated by Becton Dickinson for the selective growth and production of orange colonies of b- hemolytic (b-H) GBS. Methods 283 vaginal swabs (VAG) collected from pregnant women were inoculated in selective Lim broth. After overnight incubation, Lim broth were subcultured on GBSDA, on Granada agar (Biomedics, Spain) and on Columbia blood agar (BA). To evaluate the stability, 99 isolates of GBS (REF) from adult or neonatal infections (Belgian GBS reference laboratory collection) were cultured on GBSDA and Granada at their limit of expiration, and on BA. GBSDA and Granada were incubated anaerobically and BA aerobically + 7% CO2, at 35°C, 24 to 48 h. Positive and negative control strains (GBS ; E. faecalis) were cultured with each run. Specific identification of colonies suggestive of GBS (pale to dark orange on GBSDA and Granada, b-H on BA) was performed. Results b-H GBS were recovered from 63 VAG (22.3 %): 62 were easily identified after overnight incubation on GBSDA and 63 on Granada without requiring any subculture. All GBS were also recovered from BA however it was after many subcultures. All orange colonies were confirmed as GBS. Among REF, 3 strains were non hemolytic ; they grew but were not differentiated as orange colonies on GBSDA or Granada. 96 REF were b-H, 94 (97.9%) produced orange to very dark orange colonies on GBSDA, 2 produced white colonies, and on Granada, 74 (77.1 %) produced pale to dark orange colonies and 22 white to white-orange colonies. Conclusion 1) GBSDA and Granada: a) very high sensitivity and specificity for the detection of b-H GBS, in a single step b) Results available within 48 h after inoculation in Lim broth, low workload 2) Excellent stability up to expiration date for GBSDA 3) Non hemolytic GBS: grown but not differentiated on GBSDA or Granada. [less ▲]

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See detailEvaluation of the homocysteine assay on the Abbott IMx
Chapelle, Jean-Paul ULg; Lutteri, Laurence ULg; Gielen, J.

in Clinical Chemistry (1999), 45(6), 134

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See detailEvaluation of the hybridization between the domectic cat and the European wildcat in the Walloon region
Lambinet, Clotilde ULg; Schockert, Vincianne ULg; Libois, Roland ULg

in Lecocq, Yves (Ed.) Abstacts of 31st congress IUGB (2013, August)

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See detailEvaluation of the Hycel Diagnostics Diana-5 Hematology Analyzer
Boemer, François ULg; Cielniaszek, Nadine; Paulus, Jean-Michel ULg et al

in Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology (2002), 8

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision ... [more ▼]

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision, linearity, and absence of carryover were all excellent. All parameters were stable for 48 hours at 4 ° C and for 8 hours at room temperature. Comparability of complete blood counts to Bayer Technicon Advia 120 measurements was highly satisfactory, with the exception of the mean cell volume, which was slightly underestimated by the Diana-5 compared to the Advia 120. Comparisons of differential leukocyte counts to 400-cell microscopic differentials were excellent for neutrophils, lymphocytes, and eosinophils and were acceptable for monocytes. The efficiency of the flagging system was high for the detection of blasts and less satisfactory for the detection of immature granulocytes and atypical lymphocytes. The instrument was reliable and easy to use. [less ▲]

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See detailEvaluation of the impact of 6-month training by whole body vibration on the risk of falls among nursing home residents, observed over a 12-month period: a single blind, randomized controlled trial.
Buckinx, Fanny ULg; Beaudart, Charlotte ULg; Maquet, Didier ULg et al

in Aging Clinical & Experimental Research (2014), 26(4), 369-376

BACKGROUND: We have previously shown that short sessions of whole body vibration (WBV) were not able to significantly improve fall risk among nursing home residents but some trends towards an improvement ... [more ▼]

BACKGROUND: We have previously shown that short sessions of whole body vibration (WBV) were not able to significantly improve fall risk among nursing home residents but some trends towards an improvement of motor capacity were observed. OBJECTIVE: The objective of the present study was to evaluate the impact of 6-month training by WBV on functional and motor abilities among nursing home residents observed over a 12-month period. METHODS: Patients were randomized into two groups: the WBV group which received three training sessions every week composed of five series of 15 s of vibration at 30 Hz intensity for a period of 6 months and a control group with normal daily life. The impact of this training on the risk of falls was assessed blindly after 6 and 12 months by the Tinetti Test, the "Timed Up and Go" test and a quantitative evaluation of a 10-s walk performed with a tri-axial accelerometer. The occurrence of falls was also observed. RESULTS: 62 elderly healthy volunteers, (47 women and 15 men, mean age 83.2 +/- 7.9 years) were included in this study. There was no significant difference between the two groups regarding the Tinetti test (p = 0.75), the "Timed Up and Go" test (p = 0.19) and the Locometrix(R) test, except for the step length, measured by dual task (p < 0.01). No significant inter-group difference in the frequency of falls was observed during the 12 months of research. A total of 42 falls were recorded during the first 6 months of experimentation: 24 falls in the treated group and 18 in the control group (p = 0.60). During the next 6 months, 19 falls occurred: 8 falls in the treated group and 11 in the control group (p = 0.52). CONCLUSION: This study failed to establish the effectiveness of low doses of WBV, under the conditions used in our study, on functional and motor abilities of institutionalized elderly patients. However, given the positive results of other studies, further investigations, with modified therapeutic protocols, seem necessary to clarify the effects of WBV in the elderly. [less ▲]

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See detailEvaluation of the impact of a 6-month training by whole body vibration on the risk of falls among nursing home residents
Beaudart, Charlotte ULg; Buckinx, Fanny ULg; Demonceau, Marie ULg et al

in Osteoporosis International (2013, April), 24(Suppl.1), 246-247

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See detailEvaluation of the impact of Triclosan on the functioning of the thyroid system in Cyprinodon variegatus L., 1803.
Rahmouni, Chahrazed; Benichou, Farida; Klaren, PHM et al

Poster (2014, December)

Triclosan (5-chloro-2-[2, 4-dichlorophenoxy] phenol) is an antimicrobial widely used in various industrial products such as textiles, cosmetics and body care products. It is often detected in aquatic ... [more ▼]

Triclosan (5-chloro-2-[2, 4-dichlorophenoxy] phenol) is an antimicrobial widely used in various industrial products such as textiles, cosmetics and body care products. It is often detected in aquatic environments. The presence of the main biotransformation product, methyl TCS, indicates that this compound is not only degraded, but also persistent and accumulates in aquatic organisms. In this study, the effects of TCS on the thyroid system during embryonic and larval stages in Cyprinodon variegatus were evaluated. In particular, whole body thyroid hormone levels and the activity of deiodinases, enzymes involved in the activation of the thyroid prohormone T4, were measured. Couples of three females and two males were placed in breeding chambers designed for this experiment from which 3832 eggs were obtained by reproduction in the laboratory. Eggs were collected and maintained in seawater. Embryos were selected under a dissection microscope, randomly assigned to each of five treatment groups: Control, DMSO control (vehicle), 20 μg/L TCS, 50 μg/L TCS and 100 μg/L TCS and placed in incubation dishes (50 per dish) at 25°C. On day 6, embryos hatched and larvae were transferred to 1L dishes. The larvae were fed on artemia and on flaked fish food till day 15 post hatching when the fish were analyzed. The fertilization and survival rate, as well as the larval dry weight did not vary significantly between individuals exposed to 20, 50 and 100 μg/L TCS. T3 and T4 concentrations increased significantly in larvae exposed to 50 and 100 μg/L TCS. The study of the activity of enzymes involved in the deiodination of thyroid hormones (ORD) represents a new aspect in the study of endocrine disruption in C. variegatus. Unfortunately, we were not able to detect a net enzymatic T4 deiodination activity, most likely due to the very small amounts of protein and low specific enzyme activity in brain homogenates, carcass and liver. [less ▲]

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See detailEvaluation of the influence of age on pulmonary arterial pressure by use of right ventricular catheterization, pulsed-wave Doppler echocardiography, and pulsed-wave tissue Doppler imaging in healthy Beagles.
Mercier, Elise ULg; Mathieu, Myrielle; Sandersen, Charlotte ULg et al

in American Journal of Veterinary Research (2010), 71(8), 891-7

OBJECTIVE: To assess the influence of age on pulmonary hemodynamics and hemorheological properties in healthy dogs. ANIMALS: 14 healthy Beagles. PROCEDURES: Dogs were placed in 2 age groups as follows ... [more ▼]

OBJECTIVE: To assess the influence of age on pulmonary hemodynamics and hemorheological properties in healthy dogs. ANIMALS: 14 healthy Beagles. PROCEDURES: Dogs were placed in 2 age groups as follows: young dogs (<or= 5 years old; n = 8) and old dogs (>or= 8 years old; 6). Hematologic characteristics, plasma total protein and fibrinogen concentrations, and blood viscosity were measured. Systolic time intervals of pulmonary blood flow were recorded by pulsed-wave Doppler echocardiography. Early (E') and late (A') diastolic myocardial velocities, isovolumic contraction velocity, and systolic myocardial velocity of the free tricuspid annulus were recorded by pulsed-wave tissue Doppler imaging (TDI). Dogs were anesthetized and pulmonary arterial pressures (PAP) and cardiac output were recorded with a pediatric thermodilution Swan-Ganz catheter. RESULTS: Hemorheological measurements were not different between the 2 groups. Systolic, mean, and diastolic PAP were higher in old dogs, compared with values in young dogs; this difference was attributed to a high pulmonary vascular resistance and low arterial compliance in old dogs. Systolic time intervals of pulmonary blood flow stayed unchanged. The A' wave of the free tricuspid annulus was increased in old, compared with that young dogs, and the E':A' ratio was decreased. Pulmonary vascular resistance was inversely correlated with compliance. CONCLUSIONS AND CLINICAL RELEVANCE: The age of dogs should be taken into account when interpreting pulmonary hemodynamic results and TDI variables of right ventricular diastolic function. [less ▲]

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