Isolating the 130.4 nm and 135.6 nm emissions in Ganymede’s aurora using broadband optics

Conference (2012, September 27)

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that ... [more ▼]

We discuss a technique for isolating the two main Far Ultraviolet emission lines in Ganymede’s aurora by adding flight proven transmission filters to a broad- band, wide-field imager design. We find that the ra- tio of OI emissions at 135.6 nm and 130.4 nm can be recovered if the transmission of the filters and other optical elements are well known. This ratio allows constraints to be placed on the relative abundances of O atoms and O2 molecules within Ganymede’s at- mosphere, leading to more accurate models of atmo- spheric composition. [less ▲]

Isolation and analysis of a putative FLC orthologue in Sinapis alba L.

in Acta Physiologiae Plantarum (2004), 26(3), 23-24

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to ... [more ▼]

In many plant species, flowering is promoted by a long exposure to low temperature, a process known as vernalization. Some plants even have an absolute requirement for winter cold before being able to flower the next summer, hence behave as biennials or winter crops. A recent breakthrough in the understanding of the molecular bases of vernalization has been the cloning of the FLOWERING LOCUS C (FLC) gene in Arabidopsis thaliana (Michaels S.D. & Amasino R.M., 1999). FLC encodes a repressor of flowering and is downregulated by vernalization. So vernalization relieves the inhibitory role that FLC plays on downstream genes involved in the floral transition of the shoot apical meristem (SAM). Although vernalization has been shown, by physiological studies, to be sensed by the SAM, molecular evidences are missing. Such analyses are impaired in Arabidopsis by the small size of the plant and the rosette growth habit. We therefore cloned a FLC homologue in a caulescent relative Brassicaceae: mustard (Sinapis alba L.). We identified two clones by screening a cDNA library made from leaf mRNA. One of them, SaFLC1, was used for in situ hybridizations on SAM sections. Preliminary results are shown. Because of its agronomical importance, we have also tried to manipulate the vernalization process in crops. By using a transgenic approach, we have attempted to bypass the strict vernalization requirement of winter colza (Brassica napus L.). We have over-expressed SaMADS A, a gene which is repressed by FLC in wild type, and observed that the transgenics did not require vernalization any more. [less ▲]

Isolation and Characterisation of a Ruminant Alphaherpesvirus Closely Related to Bovine Herpesvirus 1 in a Free-Ranging Red Deer

in BMC Veterinary Research (2007), 3

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine ... [more ▼]

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population. RESULTS: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1. CONCLUSION: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses. [less ▲]

Isolation and characterisation of new spore-forming lactic acid bacteria with prospects of use in food fermentations and probiotic preparations

in African Journal of Microbiology Research [=AJMR] (2010), 4(11), 1016-1025

Isolation And Characterization Of A 2.3-Kilobase-Pair Cdna Fragment Encoding The Binding Domain Of The Bovine Leukemia-Virus Cell-Receptor

in Journal of Virology (1993), 67(2),

The isolation and characterization of equine Osteocalcin

in Proceedings of the 1st meeting of the International Bone and Mineral Society (IBMS) (2001)

Isolation and characterization of photoactive complexes of NADPH : protochlorophyllide oxidoreductase from wheat

in Planta (1998), 206(4), 673-680

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of ... [more ▼]

A photoactive substrate-enzyme complex of the NADPH:protochlorophyllide oxidoreductase (POR; EC 1.3.1.33) was purified from etiolated Triticum aestivum L. by gel chromatography after solubilization of prolamellar bodies by dodecyl-maltoside. Irradiation by a 1-ms flash induced the phototransformation of protocholorophyllide a (Pchlide) with -196 degrees C absorbance and emission maxima at 640 and 643 nm, respectively. The apparent molecular weight of this complex was 112 +/- 24 kDa, which indicates aggregation of enzyme subunits. By lowering the detergent concentration in the elution buffer, a 1080 +/- 250-kDa particle was obtained which displayed the spectral properties of the predominant form of photoactive Pchlide in vivo (-196 degrees C absorbance and fluorescence maxima at 650 and 653 nm). In this complex, FOR was the dominant polypeptide. Gel chromatography in the same conditions of an irradiated sample of solubilized prolamellar bodies indicated rapid disaggregation of the complex after Pchlide phototransformation. High performance liquid chromatographic analysis of the FOR complexes obtained using two detergent concentrations indicates a possible association of zeaxanthin and violaxanthin with the photoactive complex. [less ▲]

Isolation and characterization of respiratory NADH:ubiquinone oxidoreductase (complex I) mutants in Chlamydomonas reinhardtii

in Archives of Physiology & Biochemistry (2000), 108(Supplement 1), 10

Isolation And Chemical Evaluation Of Carob (Ceratonia Siliqua L.) Seed Germ

in Food Chemistry (2007), 102(4),

Isolation and evaluation of bacteria and fungi as biological control agents against Rhizoctonia solani.

in Communications in agricultural and applied biological sciences (2007), 72(4), 973-982