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See detailHighly pathogenic avian influenza H5N1 virus in cats and other carnivores
Thiry, Etienne ULg; Zicola, Angélique ULg; Addie, D. et al

in Veterinary Microbiology (2007), 122(1-2), 25-31

The Asian lineage highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen of birds. Only recently, the virus has been reported to cause sporadic fatal disease in carnivores, and its ... [more ▼]

The Asian lineage highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen of birds. Only recently, the virus has been reported to cause sporadic fatal disease in carnivores, and its zoonotic potential has been dominating the popular media. Attention to felids was drawn by two outbreaks with high mortality in tigers, leopards and other exotic felids in Thailand. Subsequently, domestic cats were found naturally infected and experimentally susceptible to H5N1 virus. A high susceptibility of the dog to H3N8 equine influenza A virus had been reported earlier, and recently also HPAI H5N1 virus has been identified as a canine pathogen. The ferret, hamster and mouse are suitable as experimental animals; importantly, these species are also kept as pets. Experimental intratracheal and oral infection of cats with an HPAI H5N1 virus isolate from a human case resulted in lethal disease; furthermore, cats have been infected by the feeding of infected chickens. Spread of the infection from experimentally infected to in-contact cats has been reported. The epidemiological role of the cat and other pet animal species in transmitting HPAI H5N1 virus to humans needs continuous consideration and attention. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailHighly regular polyampholytic structures adsorbed directly from solution
Mahltig, Boris; Müller-Buschbaum, P.; Wolkenhauer, M. et al

in Journal of Colloid and Interface Science (2001), 242(1), 36-43

This article concerns the adsorption of the diblock polyampholyte poly(methacrylic acid)-block-poly((dimethylamino)ethyl methary-late) (PMAA-b-PDMAEMA) from aqueous solution on silicon substrates. The ... [more ▼]

This article concerns the adsorption of the diblock polyampholyte poly(methacrylic acid)-block-poly((dimethylamino)ethyl methary-late) (PMAA-b-PDMAEMA) from aqueous solution on silicon substrates. The investigated polyampholyte is characterized by a small molecular weight around 15,000 g/mol and a big positively charged PDMAEMA block. The adsorbed amount determined by ellipsom-etry was str ongly influenced by the pH of the adsorption solution. Using dynamic light scattering polyampholytic structures with diameters around 50 nm were found in aqueous solution. The hy-drodynamic diameter was hardly affected by changing the pH of the polymer solution. Analogous regular structures were also found by scanning force microscopy (SFM) and grazing incidence, small angle X-ray scattering (GISAXS) at the silicon surface after the adsorption process. While SFM provides a topographical image of a small part of the adsorbed polyampholytic layer; GISAXS was used to get a statistical description of the lateral surface structures. The adsorbed structures were highly regular and their sizes were nearly pH independent over a lar ge pH region. Only directly at the isoelectric point of the polyampholyte larger adsorbed structures were observed. Compared with earlier investigated PMAA-b-PDMAEMA systems we are now able to prepare highly regular polyampholytic structures at silicon surfaces. There are two kinds of interactions for the adsorbed micelles. First, the charged block of the chains is directly attracted to the substrate via electrostatic interactions, while the uncharged part of the chains is only hydrophobically attracted via the hydrophobic core of the adsorbed micelle. [less ▲]

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See detailHighly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1992), 40(3), 411-9

A new method is described for locating DNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and ... [more ▼]

A new method is described for locating DNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and various non-isotopic nucleotide analogues. The labeled nucleotides bound to the surface of ultra-thin sections were then visualized by an indirect immunogold labeling technique. The resulting labeling pattern was strongly dependent on the divalent cation used in the TdT medium. The method revealed with great precision the specific DNA-containing structures within Ehrlich tumor cells, even where DNA was present in very low amounts. The method is compatible with all usual fixation and embedding procedures and can be combined with cytochemical methods. The in situ TdT method provides a very useful tool for pinpointing the precise location of DNA within biological material at the ultrastructural level. [less ▲]

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See detailHighly Specific Radioimmunoassay for Human Insulin Based on Immune Exclusion of All Insulin Precursors
Deberg, Michelle ULg; Houssa, P.; Frank, B. H. et al

in Clinical Chemistry (1998), 44(7), 1504-13

We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed ... [more ▼]

We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed, respectively, against the B10 region, the junction between A chain and C-peptide, and the junction between B chain and C-peptide), their affinity constant (approximately 10(10) L/mol), and their interactive properties in mixture. S2 and S53 were able to bind simultaneously to the same proinsulin molecule, whereas neither could bind simultaneously with S1. Preincubation of serum samples with an excess of S2 resulted in capture of proinsulin and conversion intermediates modified at the junction between B chain and C-peptide into immune complexes that no longer reacted with S1. Similarly, preincubation with S53 prevented proinsulin and conversion intermediates modified at the junction between A chain and C-peptide from reacting with S1. Preincubation with an excess of both S2 and S53 left insulin as the sole reactant with S1. Thus, separation of insulin precursors from insulin by mutually exclusive antibodies is feasible, and on the basis of this new principle, a highly specific RIA for insulin was designed. The detection limit was 11 pmol/L, and the inter- and intraassay coefficients of variation were 11% and 5%, respectively. The potential of the assay for use in clinical studies was verified by application to serum samples from control subjects and patients with diabetes or insulinoma. [less ▲]

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See detailA highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency
Turki, S.; Mrabet, G.; Jabloun, Z. et al

in Biotechnology & Applied Biochemistry (2010), 57(4), 139-149

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability ... [more ▼]

Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human GI (gastrointestinal) tract have been published. Stability of various Y. lipolytica lipase powder formulations at various physiological pH values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. Results were compared with those obtained from another competing fungal lipase sourced fromCandida rugosa. Among the studied formulations, Y. lipolytica lipase stabilized with gum arabic and skimmed milk powder was the most promising powder formulation. Under acidic conditions (pH 3-5), this formulation showed higher stability than those observed with the other Y. lipolytica lipase formulations and C. rugosa lipase. In addition, in the presence of gum arabic and skimmed milk powder as additives, Y. lipolytica lipase exhibited markedly higher resistance to pepsin, trypsin and chymotrypsin actions. Resistance to proteolytic degradation by digestive proteases was also by far higher than that observed with C. rugosa lipase. Similar behaviour was, however, observed when these two fungal lipases were incubated with increased concentrations of bile salts. Residual lipase activity of both fungal lipases showed a slight decrease in NaTDC (sodium taurodeoxycholate) concentration above 4 mM. Consequently, Y. lipolytica lipase formulated with gum arabic and milk powder seemed to have great potential for use as a therapeutic tool for patients with pancreatic insufficiency. © 2010 Portland Press Limited. [less ▲]

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See detailHighly stereoselective ruthenium-catalyzed ring-opening metathesis polymerization of 2,3-difunctionalized norbornadienes and their 7-oxa analogues
Delaude, Lionel ULg; Demonceau, Albert ULg; Noels, Alfred ULg

in Macromolecules (1999), 32(7), 2091-2103

The ring-opening metathesis polymerization (ROMP) of 2,3-difunctionalized norbornadienes and their 7-oxa analogues catalyzed by [RuCl2(p-cymene)](2) in the presence of trimethylsilyldiazomethane (TMSD ... [more ▼]

The ring-opening metathesis polymerization (ROMP) of 2,3-difunctionalized norbornadienes and their 7-oxa analogues catalyzed by [RuCl2(p-cymene)](2) in the presence of trimethylsilyldiazomethane (TMSD) yields high-trans, highly tactic polymers. A tentative mechanism involving arene loss, carbene formation, and monomer chelation is presented. The ROMP of 2,3-dicarbomethoxynorbornadiene served as a test reaction to investigate the influence of the various experimental parameters (reaction time and temperature, nature of the solvent and catalyst, and TMSD initiation conditions). An all-trans, highly tactic (>79%) polymer was obtained under a wide variety of conditions. Strikingly, the addition of tricyclohexylphosphine had a detrimental influence on the polymerization yield and stereoselectivity. The procedure was successfully applied to various other 2,3-dicarboalkoxynorbornadienes, benzonorbornadiene, and their 7-oxa analogues. In the case of the diester monomers, an increase in the alkyl substituent size led to a decrease of the polymer trans content and tacticity. Because of the ruthenium catalyst's ready availability and marked resistance to oxygen and water, reactions were very easy to set up and to carry out. [less ▲]

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See detailHighly Transient Mixed Flows with Air/Water Interactions: Homogeneous Equilibrium Model and Friction Correlations
Kerger, François ULg; Archambeau, Pierre ULg; Erpicum, Sébastien ULg et al

in Proceedings of the 8th National Congress on Theoretical and Applied Mechanics (2009, May)

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See detailHighly-Expressed P100/P52 (Nfkb2) Sequesters Other Nf-Kappa B-Related Proteins in the Cytoplasm of Human Breast Cancer Cells
Dejardin, Emmanuel ULg; Bonizzi, Giuseppina; Bellahcene, Akeila ULg et al

in Oncogene (1995), 11(9), 1835-41

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the ... [more ▼]

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the molecular control of the transformed phenotype, we examined the expression of these proteins in human breast cancer cell lines as well as in primary tumors. Western Immunoblots demonstrated high expression of the p52 precursor p100 (NFKB2) in several breast cancer cell lines while human mammary epithelial cells express this protein only faintly. Eighteen primary breast tumors out of 24 displayed significant expression of the p100/p52 protein. In MDA-MB-435 cells, overexpressed p100 and p52 are predominantly cytoplasmic and coimmunoprecipitation experiments demonstrated that p100 sequesters the heterodimer p50/p65 in the cytoplasm. We demonstrate that most p65 protein is complexed with p100 in these cells while it is complexed predominantly with I kappa B-alpha in cell lines expressing less p100. Our data strengthen the hypothesis that NF-kappa B could be involved in carcinogenesis and suggest that the p100/p52 NF-kappa B subunit could play a role in the development of human breast cancers, possibly by sequestering other NF-kappa B-related proteins in the cytoplasm. [less ▲]

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See detailL’hijab, “voile islamique” aspects juridiques, administratifs et pratiques
Husson, Jean-François ULg

Conference given outside the academic context (2008)

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See detailHilde Domin
Viehöver, Vera ULg

Book published by Wehrhahn Verlag (2010)

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques
Veymiers, Richard ULg

Scientific conference (2012, November 30)

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques
Veymiers, Richard ULg

Book published by Académie Royale de Belgique (2009)

Après la conquête de l’Égypte par Alexandre le Grand, la déesse Isis se voit attacher, probablement à l’initiative de Ptolémée Sôter, un nouvel époux du nom de Sérapis, qui n’est autre que l’Osiris-Apis ... [more ▼]

Après la conquête de l’Égypte par Alexandre le Grand, la déesse Isis se voit attacher, probablement à l’initiative de Ptolémée Sôter, un nouvel époux du nom de Sérapis, qui n’est autre que l’Osiris-Apis memphite transféré à Alexandrie pour y recevoir une image nouvelle, purement grecque. Cette entreprise marque le point de départ d’un phénomène que nous appelons aujourd’hui la diffusion des cultes isiaques. Sept siècles durant, Sérapis et les siens essaiment dans le monde gréco-romain et attirent une audience de plus en plus large et diversifiée avant de succomber au triomphe du christianisme. Cette diffusion s’opère en fonction de vecteurs variés et notamment par le biais de supports matériels. Au même titre que les monnaies dans le domaine public, les objets de dimensions modestes que sont les gemmes et les bijoux favorisent le développement des cultes isiaques dans les sphères privées en véhiculant une iconographie multiple. C’est ainsi que Pline l’Ancien observe que, de son temps, « même les hommes commencent à porter à leurs doigts l’effigie d’Harpocrate et les images des divinités égyptiennes ». Cette vogue dont témoigne le grand érudit romain n’a toutefois guère éveillé l’intérêt des chercheurs pour cette documentation. Cet ouvrage vise à montrer combien ces objets, malgré leur petite taille, sont précieux pour nous aider à comprendre ce que sont les cultes isiaques et à mesurer l’étendue de leur succès. Il s’organise autour d’un catalogue réunissant plus de 1250 documents, souvent méconnus ou inédits, portant l’image ou le nom de Sérapis. Les gemmes et les bijoux montrent une richesse iconographique, jusqu’ici insoupçonnée, qui a permis d’élaborer une fine typologie. Traditionnellement considéré comme un maître des ombres trônant avec le Cerbère infernal, Sérapis y apparaît, plus que sur tout autre support, comme un être insaisissable, aux apparences multiples, en buste, trônant, debout ou allongé, qu’il soit seul, accompagné ou assimilé à d’autres divinités. Si les artisans, graveurs et bijoutiers, se sont inspirés de schémas iconographiques circulant dans le domaine public, en particulier sur les monnaies, ils ont aussi innové pour exprimer des sentiments personnels ou répondre aux attentes de leur clientèle. Percer le sens de ces images est une entreprise parfois aussi difficile que de déterminer les raisons du port de ces objets. Si certains ont pu simplement servir de parure, de sceau ou de souvenir, d’autres ont été destinés à afficher une adhésion religieuse, à solliciter une protection ou à satisfaire une pratique magique. Redécouvrir ces gemmes et ces bijoux ne peut manquer de nous toucher parce qu’ils ont été portés durant l’Antiquité par des individus ordinaires qui ont attendu, d’une manière ou d’une autre, que Sérapis soit Hileôs tôi phorounti, « propice au porteur ». [less ▲]

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques
Veymiers, Richard ULg

Doctoral thesis (2008)

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques
Veymiers, Richard ULg

Scientific conference (2009, April 06)

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques. Supplément I
Veymiers, Richard ULg

in Veymiers, Richard; Bricault, Laurent (Eds.) Bibliotheca Isiaca II (2011)

Présentation d'une centaine de documents tout récemment publiés ou totalement inédits, qui viennent compléter les 1250 gemmes et bijoux répertoriés dans notre ouvrage "Hileôs tôi phorounti. Sérapis sur ... [more ▼]

Présentation d'une centaine de documents tout récemment publiés ou totalement inédits, qui viennent compléter les 1250 gemmes et bijoux répertoriés dans notre ouvrage "Hileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques" (Bruxelles, 2009). [less ▲]

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See detailHileôs tôi phorounti. Sérapis sur les gemmes et les bijoux antiques. Supplément II
Veymiers, Richard ULg

in Bricault, Laurent; Veymiers, Richard (Eds.) Bibliotheca Isiaca III (in press)

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See detail"Hilf dir selbst, dann hilft dir Gott"? Eine politische Geschichte der Deutschsprachigen Gemeinschaft Belgiens
Brüll, Christoph ULg

in Begenat-Neuschäfer, Anne (Ed.) Die Deutschsprachige Gemeinschaft Belgiens. Eine Bestandsaufnahme (2010)

"Minorité la mieux protégée" d'Europe et "derniers Belges" ne sont que deux des stéréotypes qu'on relie à la plus petite entité fédérée de l'État fédéral belge. La réalité historique et politique derrière ... [more ▼]

"Minorité la mieux protégée" d'Europe et "derniers Belges" ne sont que deux des stéréotypes qu'on relie à la plus petite entité fédérée de l'État fédéral belge. La réalité historique et politique derrière ces clichés n'est cependant pas très bien connue. Cette contribution tente de retracer le parcours vers une autonomie politique pour les Belges germanophones et l'évolution politique depuis les années 1960. Sans le processus de fédéralisation de la Belgique qui conditionnait la vie politique belge, sans le conflit entre Flamands et Wallons, on n'aurait jamais assisté à la création d'une communauté pour quelque 75.000 habitants. Les réalisations politiques concrètes de l'autonomie sont également anaylsées, tout comme le champ de tension entre l'autonomie et la petite taille du territoire. Il en ressort également que le chemin entamé n'est pas encore arrivé à sa fin et est intimement lié à l'évolution future de l'État belge. [less ▲]

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See detail"Hilf dir selbst, so hilft dir Gott!" Verzerrende Geschichtsbilder und fehlendes Selbstbewusstsein
Brüll, Christoph ULg; Cremer, Freddy; Mießen, Werner

in Lejeune, Carlo; Brüll, Christoph (Eds.) Grenzerfahrungen. Eine Geschichte der Deutschsprachigen Gemeinschaft Belgiens. Vol. 5: Säuberung, Wiederaufbau, Autonomiediskussionen (1945-1973) (2014)

Das unitaristische und zentralistische Belgien war in Brüssel oder Löwen schon in den 1960er Jahren nur noch ein Trugbild. Das Land war spürbar im Aufbruch. Doch diese seismographischen Wellen wurden in ... [more ▼]

Das unitaristische und zentralistische Belgien war in Brüssel oder Löwen schon in den 1960er Jahren nur noch ein Trugbild. Das Land war spürbar im Aufbruch. Doch diese seismographischen Wellen wurden in den Ostkantonen kaum registriert. Die innerbelgischen Autonomiediskussionen, auf die Ende der 1960er Jahre schließlich auch die Deutschsprachigen eine Antwort finden sollten, überwältigten diese traumatisierte Minderheit. Hat sie ihre Autonomie wirklich erkämpft oder ist diese das Nebenprodukt der sich stets verschärfenden Auseinandersetzungen zwischen den beiden großen Volksgruppen in Belgien? Welche Interessengruppen haben das demokratische Miteinander mit welchen Zielen bestimmt? Sie liefern Einsichten in einen komplexen Aspekt der politischen Geschichte Ostbelgiens, wobei sie geschickt die Außenperspektive nutzen, um die Mechanismen in Ostbelgien in ein neues Licht zu stellen. Ausgehend von (so die Titel) „Verzerrenden Geschichtsbildern und fehlendem Selbstbewusstsein“ überschreiben sie die spannende Reise in die demokratischen Anfänge der heutigen Deutschsprachigen Gemeinschaft mit der markanten Aussage von Leo Tindemans „Hilf dir selbst, so hilft dir Gott!“ [less ▲]

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