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See detailIdentification of surf actin producing strains in Soumbala and Bikalga fermented condiments using polymerase chain reaction and matrix assisted laser desorption/ionization-mass spectrometry methods
Savadogo, A; Tapi, Arthur; Chollet, Marlène et al

in International Journal of Food Microbiology (2011)

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See detailIdentification of tenuis of four French Polynesian Carapini (Carapidae : Teleostei)
Parmentier, Eric ULg; Lo-Yat, A.; Vandewalle, Pierre ULg

in Marine Biology (2002), 140(3), 633-638

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to ... [more ▼]

Four species of adult Carapini (Carapidae) occur on Polynesian coral reefs: Encheliophis gracilis, Carapus boraborensis, C. homei and C. mourlani. Samples collected in Rangiroa and Moorea allowed us to obtain different tenuis (larvae) during their settlement phases or directly inside their hosts. These were separated into four lots on the basis of a combination of pigmentation, meristic, morphological, dental and otolith (sagittae) features. Comparison of these characters with those of the adults allows, for the first time, taxonomic identification of these tenuis-stage larvae. [less ▲]

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See detailIdentification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein.
Clausse, Nathalie; Jackers, Pascale ULg; Jares, P. et al

in DNA & Cell Biology (1996), 15(12), 1009-23

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents ... [more ▼]

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents intriguing multifunctional properties because it has been characterized as the precursor of the metastasis-associated 67-kD laminin receptor (67LR) and as a cytoplasmic ribosomal-associated protein. The isolation of the 37LRP/p40 gene is a prerequisite for identifying the molecular mechanisms responsible for the constant up-regulation of the 67LR expression in cancer cells. To date, the active 37LRP/p40 gene has never been identified in any species due to the existence of multiple pseudogenes in most vertebrates genomes. In this study, we report for the first time the gene structure and potential regulatory sequences of the 37 LRP/p40 gene. The chicken genome was selected to undergo this characterization because it is the only known vertebrate that bears a single 37 LRP/p40 gene copy. The 37 LRP/p40 active gene is composed of 7 exons and 6 introns and bears features characteristic of a ribosomal protein gene. It does not bear a classical TATA box and it exhibits several transcription initiation sites as demonstrated by RNase protection assay and primer extension. Analysis of potential regulatory regions suggests that gene expression is driven not only by the 5' genomic region but also by the 5' untranslated and intron 1 sequences. On the basis of gene structure and extensive protein evolutionary study, we found that the carboxyterminal domain of the protein is a conserved lock-and-key structure/function domain that could be involved in the biosynthesis of the higher-molecular-weight 67-kD laminin receptor in vertebrates, whereas the central core of the protein would be responsible for the ribosome associated function. The first identification of the active 37LRP/p40 gene presented in this study is a critical step toward the isolation of the corresponding human gene and the understanding of the molecular mechanisms involved in the up-regulation of its expression during tumor invasion and metastasis. [less ▲]

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See detailIdentification of the bovine leukemia virus transactivating protein (p34x).
Willems, Luc ULg; Kettmann, Richard ULg; Portetelle, Daniel ULg et al

in Haematology and Blood Transfusion (1987), 31(1), 482-7

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See detailIdentification of the cis element recognized by a transcription factor specific to the tumor BT-474 mammary cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Hodzic, Didier et al

Conference (1996, February 03)

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See detailIdentification of the Core Structure of Lysozyme Amyloid Fibrils by Proteolysis
Frare, Erica; Mossuto, Maria F.; Polverino de Laureto, Patrizia et al

in Journal of Molecular Biology (2006), 361

Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be ... [more ▼]

Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be induced to form amyloid fibrils when incubated under appropriate conditions. In this study, fibrils of wild-type human lysozyme formed at low pH have been analyzed by a combination of limited proteolysis and Fouriertransform infrared (FTIR) spectroscopy, in order to map conformational features of the 130 residue chain of lysozyme when embedded in the amyloid aggregates. After digestion with pepsin at low pH, the lysozyme fibrils were found to be composed primarily of N and C-terminally truncated protein species encompassing residues 26–123 and 32–108, although a significant minority of molecules was found to be completely resistant to proteolysis under these conditions. FTIR spectra provide evidence that lysozyme fibrils contain extensive β-sheet structure and a substantial element of non β-sheet or random structure that is reduced significantly in the fibrils after digestion. The sequence 32–108 includes the β-sheet and helix C of the native protein, previously found to be prone to unfold locally in human lysozyme and its pathogenic variants. Moreover, this core structure of the lysozyme fibrils encompasses the highly aggregation-prone region of the sequence recently identified in hen lysozyme. The present proteolytic data indicate that the region of the lysozyme molecule that unfolds and aggregates most readily corresponds to the most highly protease-resistant and thus highly structured region of the majority of mature amyloid fibrils. Overall, the data show that amyloid formation does not require the participation of the entire lysozyme chain. The majority of amyloid fibrils formed from lysozyme under the conditions used here contain a core structure involving some 50% of the polypeptide chain that is flanked by proteolytically accessible N and C-terminal regions. [less ▲]

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See detailIdentification of the dairy cows treated with bovine somatotropine (BST): criteria to discriminate the treated animals from the non-treated ones
Scippo, Marie-Louise ULg; Degand, Guy ULg; Maghuin-Rogister, Guy ULg

in Second meeting on animal productions. The milk channel], Centre de Recherches Agronomiques, Gembloux (Belgium); Faculte Universitaire des Sciences Agronomiques de Gembloux (Belgium).- Gembloux (Belgium), 1997. p. Q3 (1997)

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See detailIdentification of the different sources responsible for olfactory annoyance, using an e-nose
clarke, Kevin; Redon, Nathalie; Locoge, Nadine et al

in Perena, Gouma, (Ed.) OLFACTION AND ELECTRONIC NOSE: PROCEEDINGS OF THE 14TH INTERNATIONAL SYMPOSIUM ON OLFACTION AND ELECTRONIC NOSE (2011, May)

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See detailIdentification of the F17 fimbrial subunit-and adhesin-encoding (F17A and F17G) gene variants in necrotoxigenic Escherichia coli
Mainil, Jacques ULg; Gérardin, J.; Jacquemin, E.

in Veterinary Microbiology (2000), 73

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See detailIdentification of the linear parts of nonlinear systems for fuzzy modeling
Rezaei Sadrabadi, Mahmood ULg

in Applied Soft Computing (2011), 11

In direct approach to fuzzy modeling, structure identification is one of the most critical tasks. In modeling the nonlinear system, this fact is more crucial. In this paper, a new hybrid method is ... [more ▼]

In direct approach to fuzzy modeling, structure identification is one of the most critical tasks. In modeling the nonlinear system, this fact is more crucial. In this paper, a new hybrid method is proposed to cluster the data located in the linear parts on the nonlinear systems. The proposed method can partition the input–output data in two groups: data located in the linear parts and data in the extrema. It is shown that the first group of data is suitable to be clustered by Fuzzy C-Regression Model (FCRM) clustering algorithm and the second group by Fuzzy C-Means (FCM). Then, based on the above findings, a new hybrid clustering algorithm is proposed. Finally, the proposed approach is tested and validated by several numerical examples of nonlinear functions. [less ▲]

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See detailIdentification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family
Xie, Sancai; Low, Boon G.; Nagel, Robert J. et al

in Proceedings of the National Academy of Sciences of the United States of America (1991), 88(22), 10247-10251

Pregnancy in cattle and sheep can be diagnosed by the presence of a conceptus-derived antigen in maternal serum that is secreted by trophoblast and placental tissue primarily as an acidic component of Mr ... [more ▼]

Pregnancy in cattle and sheep can be diagnosed by the presence of a conceptus-derived antigen in maternal serum that is secreted by trophoblast and placental tissue primarily as an acidic component of Mr 67,000. Molecular cloning of its cDNA reveals that the antigen belongs to the aspartic proteinase family and has greater than 50% amino acid sequence identity to pepsin, cathepsin D, and cathepsin E. The inferred sequences of the ovine and bovine polypeptides show approximately 73% identity to each other. Critical amino acid substitutions at the active site regions suggest that both proteins are enzymatically inactive. The antigen is a product of trophoblast binucleate cells that invade maternal endometrium at implantation sites. [less ▲]

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See detailIdentification of the Mechanical and Piezoelectric Parameters of a Massively Piezoelectric Structure
De Boe, P.; Pascual, R.; Golinval, Jean-Claude ULg

Conference given outside the academic context (1998)

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See detailIdentification of the nonlinear function in a vibrating nonlinear beam
Dimitriadis, Grigorios ULg; Cooper, Jonathan E

in Lees, A. W.; Prells, U.; Norton, J. (Eds.) Proceedings of the Third International Conference on Identification in Engineering Systems (2002, April)

The Constant Level Identification (CLI) method for nonlinear dynamical systems has been shown to identify accurately a wide variety of systems with discrete degrees of freedom. In this paper, the method ... [more ▼]

The Constant Level Identification (CLI) method for nonlinear dynamical systems has been shown to identify accurately a wide variety of systems with discrete degrees of freedom. In this paper, the method is applied to a continuous nonlinear system, namely a simulated model of a vibrating beam attached to a nonlinear spring. A Rayleigh-Ritz series representation of the beam is assumed in order to extract the desired number of modal responses. Then, a combination of modal and physical coordinates are used in the identification process. It is shown that the CLI method can accurately capture the shape of the nonlinearity in the system. [less ▲]

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See detailIdentification of the Origin of Catecholaminergic Inputs to Hvc in Canaries by Retrograde Tract Tracing Combined with Tyrosine Hydroxylase Immunocytochemistry
Appeltants, D.; Absil, Philippe ULg; Balthazart, Jacques ULg et al

in Journal of Chemical Neuroanatomy (2000), 18(3), 117-33

The telencephalic nucleus HVc (sometimes referred to as the high vocal center) plays a key role in the production and perception of birdsong. Although many afferent and efferent connections to this ... [more ▼]

The telencephalic nucleus HVc (sometimes referred to as the high vocal center) plays a key role in the production and perception of birdsong. Although many afferent and efferent connections to this nucleus have been described, it has been clear for many years, based on chemical neuroanatomical criteria, that there are projections to this nucleus that remain undescribed. A variety of methods including high performance liquid chromatography, immunohistochemistry and receptor autoradiography have identified high levels of catecholamine transmitters, the presence of enzymes involved in the synthesis of catecholamines such as tyrosine hydroxylase and a variety of catecholamine receptor sub-types in the HVc of several songbird species. However, no definitive projections to HVc have been described from cells groups known to synthesize catecholamines. These projections were analyzed in the present study by retrograde tract tracing combined with immunocytochemistry for tyrosine hydroxylase. The origin of the catecholaminergic inputs to HVc were determined based exclusively on birds in which injections of the retrograde tracer (latex fluospheres) were confined within the cytoarchitectonic boundaries of the nucleus. Retrogradely transported latex fluospheres were found mainly in cells of two dopaminergic nuclei, the mesencephalic central gray (A11) and, to a lesser extend, the area ventralis of Tsai (A10; homologous to the ventral tegmental area of mammals). A few retrogradely-labelled cells were also found in the noradrenergic nucleus subceruleus (A6). Most of these retrogradely-labelled cells were also tyrosine hydroxylase-positive. Other catecholaminergic nuclei were devoid of retrograde label. These data converge with others studies to indicate that HVc receives discrete dopaminergic and noradrenergic inputs. These inputs may influence the steroid regulation of HVc, attentional processes related to song and modulate sensory inputs to the song system. [less ▲]

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See detailIdentification of the parameters of a damage constitutive law for steel at elevated temperature
Castagne, S.; Habraken, Anne ULg

in Banabic, D. (Ed.) Proceedings of the 8th ESAFORM Conference on Material Forming (2005)

This paper presents the methodology and the results of the calibration of an interface damage law using experimental damage analyses and associated finite element simulations. The experimental damage ... [more ▼]

This paper presents the methodology and the results of the calibration of an interface damage law using experimental damage analyses and associated finite element simulations. The experimental damage analyses consist in acoustic tests realised on steel samples in order to determine the apparition of the first crack during compression. Finite elements simulations of these experiments allow the determination of the damage parameters using a reverse method. [less ▲]

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See detailIdentification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages.
Godfroid, F.; Taminiau, Bernard ULg; DANESE, Isabelle ULg et al

in Infection and Immunity (1998), 66(11), 5485-93

Brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. The smooth lipopolysaccharide (S-LPS) has been reported to be an important virulence ... [more ▼]

Brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. The smooth lipopolysaccharide (S-LPS) has been reported to be an important virulence factor of these organisms, but the genetic basis of expression of the S-LPS O antigen has not yet been described. Likewise, the role of the O side chain of S-LPS in the survival of Brucella has not been clearly defined. A mini-Tn5 transposon mutant library of Brucella melitensis 16M was screened by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) directed against the O side chain of Brucella. One mutant, designated B3B2, failed to express any O side chain as confirmed by ELISA, Western blot analysis, and colony coloration with crystal violet. Nucleotide sequence analysis demonstrated that the transposon disrupted an open reading frame with significant homology to the putative perosamine synthetase genes of Vibrio cholerae O1 and Escherichia coli O157:H7. The low G+C content of this DNA region suggests that this gene may have originated from a species other than a Brucella sp. The survival of B. melitensis mutant strain B3B2 in the mouse model and in bovine macrophages was examined. The results suggested that S-LPS or, more precisely, its O side chain is essential for survival in mice but not in macrophages. [less ▲]

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See detailIdentification of the post-maximum lines in the spectrum of Nova (RS) Ophiuchi.
Joy, A. H.; Swings, Polydore ULg

in Astrophysical Journal (1945), 102

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS ... [more ▼]

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS) Ophiuchi obtained between September 2 and November 10, 1933, shows many plausible identifications of lines which were impossible earlier. The atoms now identified, in addition to H, are: He I, He II, C III, [N II], N m, [O I], O III, [O III], [Ne III], [Ne IV], Na I, Si I, Si II, Si III, Si IV, [S II], [S III], [A V], [A X], [A XI], [K IV], [K V], [Ca V], [Ca VI], [Ca VII], [Ca XIII], [Sc VII], [V VIII], Fe II, [Fe II], [Fe IV], [Fe V], [Fe VI], [Fe VII], [Fe X], [Fe XI], [Fe XIV], [Ni XII], [Kr III]. Of these, [A XI] λ 6919, [Ca VII] λ 3688 (and possibly [V VIII] λ 3686), [Sc VII] λ 4823, and [Kr III] λ 6827 are identified for the first time. Considerations of the physical conditions in the solar corona as compared with those in the recurrent novae indicate marked differences, the coronal strata not permitting radiation of forbidden lines in the lower stages of ionization such as are found in the novae. [less ▲]

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See detailIdentification of the post-maximun lines in the spectrum of nova (RS) Ophiuchi.
Joy, A.-H.; Swings, Polydore ULg

in Contributions from the Mount Wilson Observatory (1945), 714

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS ... [more ▼]

Interest in the spectra of recurring novae has been aroused by the recent (fourth) outburst of Nova (T) Pyxidis. A review of the hitherto unpublished measures of the Mount Wilson spectrograms of Nova (RS) Ophiuchi obtained between September 2 and November 10, 1933, shows many plausible identifications of lines which were impossible earlier. The atoms now identified, in addition to H, are: He I, He II, C III, [N II], N III, [O I], O III, [O III], [Ne III], [Ne IV], Na I, Si I, Si II, Si III, Si IV, [S II], [S III], [A V], [A X], [A XI], [K IV], [K V], [Ca V], [Ca VI], [Ca VII], [Ca XIII], [Sc VII], [V VIII], Fe II, [Fe II], [Fe IV], [Fe V], [Fe VI], [Fe VII], [Fe X], [Fe XI], [Fe XIV], [Ni XII], [Zr III]. Of these, [A XI] λ 6919, [Ca VII] λ 3688 (and possibly [V VIII] λ 3686), [Sc VII] λ 4823, and [Kr III] λ 6827 are identified for the first time. Considerations of the physical conditions in the solar corona as compared with those in the recurrent novae indicate marked differences, the coronal strata not permitting radiation of forbidden lines in the lower stages of ionization such as are found in the novae. [less ▲]

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