Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailEvaluation of the quantitative performances of Supercritical Fluid Chromatography : from method development to validation
Dispas, Amandine ULg; Lebrun, Pierre ULg; Ziemons, Eric ULg et al

in Journal of Chromatography. A (2014), 1353(Method Validation), 78-88

Recently, the number of papers about SFC increased drastically but scientists did not truly focus their work on quantitative performances of this technique. In order to prove the potential of UHPSFC, the ... [more ▼]

Recently, the number of papers about SFC increased drastically but scientists did not truly focus their work on quantitative performances of this technique. In order to prove the potential of UHPSFC, the present work discussed about the different steps of the analytical life cycle of a method: from development to validation and application. Moreover, the UHPSFC quantitative performances were evaluated in comparison with UHPLC, which is the main technique used for quality control in the pharmaceutical industry and then could be considered as a reference. The methods were developed using Design Space strategy, leading to the optimization of robust method. In this context, when the Design Space optimization shows guarantee of quality, no more robustness study is required prior to the validation. Then, the methods were geometrically transferred in order to reduce the analysis time. The UHPSFC and UHPLC methods were validated based on the total error approach using accuracy profile. Even if UHPLC showed better precision and sensitivity, UHPSFC method is able to give accurate results in a dosing range larger than the 80–120% range required by the European Medicines Agency. Consequently, UHPSFC results are valid and could be used for the control of active substance in a finished pharmaceutical product. Finally, UHPSFC validated method was used to analyse real samples and gave similar results than the reference method (UHPLC). [less ▲]

Detailed reference viewed: 79 (27 ULg)
Peer Reviewed
See detailEVALUATION OF THE RAPID DETECTION OF ST-17 AND ST-1 GROUP B STREPTOCOCCI USING A MICROFLEX MALDI-TOF MS (BRUKER)
MEEX, Cécile ULg; SACHELI, Rosalie ULg; DESCY, Julie ULg et al

Poster (2014, May)

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to ... [more ▼]

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to evaluate an easy and rapid method, recently described to detect ST-17 and ST-1 GBS, based on distinguishing peak-shifts present on the protein spectrum of these 2 sequence types, using a Microflex (Bruker) matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS). Methods This study was performed on 67 multi locus sequence typed (MLST) GBS originated from the Belgian and Czech National Reference Centers, including 18 ST-17 and 16 ST-1. After culture on blood agar, an ethanol/formic acid extraction was performed on each strain. Each extract was spotted once on a target plate, overlaid with 1 µl alpha-cyano-4-hydroxycinnamic acid matrix and further analysed by a Microflex MALDI-TOF MS. One spectrum per isolate was recorded, 240 laser shots being recorded for each spectrum. The spectra were further analysed using a Bruker prototype software, and 2 logarithmic values, one for ST-17 and one for ST-1, calculated from the intensities of the present and absent peaks, were obtained for each strain. If >0, this value indicated the presence of the specific sequence type. In a second step, the test was repeated on each strain with discordant result when compared with MLST. Results Compared with MLST method, the first analysis of the strains gave poor results, leading to very low sensitivities (77.8% for ST-17 and 50% for ST-1) but rather good specificities (85.7% for ST-17 and 98.0% for ST-1). After repeating the analysis on the strains with discordant result, sensitivity, 100% and 93.8%, and specificity, 87.8% and 98.0%, for ST-17 and ST-1 respectively were highly improved. Conclusion Since ST-17 and ST-1 GBS both show distinguishing peak-shifts on their protein spectrum, as described by Lartigue et al., the distinction of these 2 sequence types is now possible by MALDI-TOF MS. To our knowledge, this study is the first describing this application on a Microflex MS using a software to classify the strains. The observed results are promising but, given to the variability of the logarithmic value given by the software, the need to perform several measures on a same strain seems to be essential. After optimization of the analysis procedure, this rapid, easy and cheap method could be used to precociously detect ST-17 among GBS isolated from prenatal screenings, allowing a better follow up of the colonized mothers and a closer monitoring of their newborns. We would like to thank the Bruker Company which allowed us to evaluate the prototype software they have developed. [less ▲]

Detailed reference viewed: 91 (22 ULg)
Full Text
See detailEvaluation of the regional climate model WRF over Svalbard
Lang, Charlotte ULg; Fettweis, Xavier ULg; Doutreloup, Sébastien ULg et al

Poster (2012, April 24)

It is well known that high latitude zones are very sensitive to climate change. As a result of global warming, ice sheet melting has increased which in turn has an influence on climate through ... [more ▼]

It is well known that high latitude zones are very sensitive to climate change. As a result of global warming, ice sheet melting has increased which in turn has an influence on climate through modifications of the thermohaline circulation, feedback of ice albedo, sea level rise, … Svalbard is an archipelago between 74 and 81°lat N and 60 percent of its area (62 248 km2) is covered with glaciers and ice sheets. The impact of global warming on the Svalbard cryosphere can be estimated with climate models. However, we need to use regional climate models as they offer the possibility of a higher resolution than general circulation models. We have ran two regional climate models (MAR and WRF) at a 10-kilometre resolution between 2006 and 2010 over Svalbard and compared their simulated climate to near surface measurements at several weather stations through the archipelago in order to determine which one of them could best represent the Svalbard climate. [less ▲]

Detailed reference viewed: 75 (8 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the relationship between IGF-1, IGF-BP3, BMD and age in men presenting at a multiple risk detection campaign
Hanssens, L.; Tancredi, Annalisa ULg; DeCeulaer, F. et al

in Osteoporosis International (2004, May), 15(Suppl.1), 48-49

Detailed reference viewed: 21 (11 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the relationship between IGF-I, IGF-BP3, BMD and age in men presenting at a multiple risk detection campaign
Hanssens, L.; Tancredi, Annalisa ULg; De Ceulaer, F. et al

in Osteoporosis International (2003, November), 14(Suppl. 7), 90-91

Detailed reference viewed: 31 (10 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the relationships between characteristics of the vertebral column of different cetaceans and their ecology: A preliminary study.
Gillet, Amandine ULg; Ninane, Catherine ULg; Remy, François ULg et al

Poster (2014, April)

Ecomorphology is the study of the relationships between functional design and the environment. One of its aims is to understand how the environmental factors can constraint the performance of an organism ... [more ▼]

Ecomorphology is the study of the relationships between functional design and the environment. One of its aims is to understand how the environmental factors can constraint the performance of an organism or act on its phenotype. Different studies have already showed in different cetaceans that the number and shape of vertebrae can reflect the stiffness of the body and consequently can impact their swimming mode. The aim of this preliminary study is to establish relationships between characteristics of the vertebral column of different cetaceans and their ecology. To this purpose, we have studied meristic and morphometric data on the vertebrae (centrum length, height and width, neural and haemal spine height and the transverse process length) of different species of mysticetes and odontocetes coming from the Aquarium-Museum of Liège and Royal Institute of Natural Sciences of Bruxelles. Preliminary results showed the distinction of three morphotypes: firstly, the active, cruising, fast swimmers with rigid body, secondly, the maneuverers, slow swimmers with flexible body and thirdly, the steady swimmers. [less ▲]

Detailed reference viewed: 53 (19 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the residual solvent content of counterfeit tablets and capsules
Deconinck, Eric; Canfyn, Michael; Sacre, Pierre-Yves ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2013), 81-82

A group of counterfeit samples of Viagra® and Cialis® were screened for their residual solvent content and compared to the content of the genuine products. It was observed that all counterfeit samples had ... [more ▼]

A group of counterfeit samples of Viagra® and Cialis® were screened for their residual solvent content and compared to the content of the genuine products. It was observed that all counterfeit samples had higher residual solvent contents compared to the genuine products. A more diverse range of residual solvents was found as well as higher concentrations. In general these concentrations did not exceed the international imposed maximum limits. Only in a few samples the limits were exceeded. A Projection Pursuit analysis revealed clusters of samples with similar residual solvent content, possibly enabling some future perspectives in forensic research. [less ▲]

Detailed reference viewed: 66 (9 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Response of Shear Critical Walls Using a Three-Parameter Kinematic Theory
Mihaylov, Boyan ULg; Hannewald, Pia; Beyer, Katrin

in The 4th International fib Congress 2014 Mumbai - Proceedings (2014, February)

This paper discusses a newly developed three-parameter kinematic theory (3PKT) for shear critical walls with the help of four wall tests. The 3PKT is used to predict the pre- and post- peak response of ... [more ▼]

This paper discusses a newly developed three-parameter kinematic theory (3PKT) for shear critical walls with the help of four wall tests. The 3PKT is used to predict the pre- and post- peak response of the test units. Comparisons are performed with finite element (FE) models and plastic hinge models in combination with a shear degradation model. It is found that the latter underestimate the displacement capacity of the walls while the former do not predict well the post-peak response. The 3PKT with only three degrees of freedom captures the complete response of the walls provided that the size of the critical loading zone (CLZ) of the wall is well predicted. [less ▲]

Detailed reference viewed: 105 (20 ULg)
Peer Reviewed
See detailEvaluation of the sensitivity of the terminal deoxynucleotidyl transferase-immunogold technique on Balbani ring genes.
Thiry, Marc ULg; Daneholt, B.

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1998), 46(3), 345-51

Recently, we developed the terminal deoxynucleotidyl transferase (TdT)-immunogold technique for in situ detection of DNA molecules. In this study the potential value and the limitations of the method were ... [more ▼]

Recently, we developed the terminal deoxynucleotidyl transferase (TdT)-immunogold technique for in situ detection of DNA molecules. In this study the potential value and the limitations of the method were evaluated using the giant polytene chromosomes from Chironomus tentans salivary glands. Emphasis was put on the Balbiani rings (BRs), specialized chromosomal sites with exceptionally intense synthesis of large mRNA molecules. Immunolabeling was recorded not only over the bands and interbands of the polytene chromosomes but also over the BR structures. In the BRs, gold particles were present over segments of active transcription units, each with a central chromatin axis and a number of growing RNP products attached to the axis. One third of the transversely sectioned transcription units showed labeling in the central parts, i.e., where the unfolded chromatin axis is located, whereas the growing RNP fibers remained unlabeled. The absence of labeling of the RNP fibers is not likely to be due to lack of accessibility, because anti-RNA antibodies readily decorated the RNP fibers. The nuclear sap and cytoplasm displayed no significant label. These results clearly indicate that the TdT-immunogold technique is specific for DNA and detects not only DNA in compacted chromatin but also fully extended DNA. Its ability to efficiently label a single DNA molecule demonstrates the method's very high sensitivity. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
See detailEvaluation of the sensory quality of beef patties inoculated with Carnobacterium maltaromaticum strains with biopreservative potential
Didimo Imazaki, Pedro Henrique ULg; Jacques-Houssa, Charlotte ULg; Kergourlay, Gilles ULg et al

Conference (2015, October 16)

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to ... [more ▼]

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to perform a sensory evaluation of beef patties inoculated with potentially biopreservative strains of Carnobacterium maltaromaticum. Three different strains of C. maltaromaticum (lab. ref.: CM_824, CM_827 and CM_829) isolated from vacuum packaged beef with long shelf life were selected for this study. An untrained panel was requested to make a sensory evaluation of raw and cooked beef patties 8 and 10 days after inoculation with the selected strains at 104 and 106 UFC/g and storage in high-O2 atmosphere. After 8 days of storage, non inoculated samples (blank) were perceived as having the best studied sensory descriptors. The samples inoculated with strain CM_827 had a sensory quality very close to the blank. After 10 days of storage, samples inoculated with the strain CM_827 at 104 UFC/g received the highest scores for appearance and color. This study permitted to evaluate the effect of three C. maltaromaticum strains on the sensory quality of beef patties. Strain CM_827 did practically not change the sensory attributes of beef patties. Samples inoculated with strain CM_824 and CM_829 received the worst scores for several of the tested descriptors. Therefore, further research on the biopreservative capacity of C. maltaromaticum should be conducted with strain CM_827. [less ▲]

Detailed reference viewed: 13 (3 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the shedding potential of textile materials
De Wael, K.; Lepot, Laurent ULg; Lunstroot, K. et al

in Science & Justice : Journal of the Forensic Science Society (2010), 50(4), 192-194

A practical method is proposed to assess the shedding potential of textile materials. The evaluation of the sheddability is based on the use of adhesive tapes and on comparison, using three different ... [more ▼]

A practical method is proposed to assess the shedding potential of textile materials. The evaluation of the sheddability is based on the use of adhesive tapes and on comparison, using three different sheddability scales for cotton, wool and fine man-made fibres. (c) 2010 Forensic Science Society. Published by Elsevier Ireland Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 31 (2 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Simple Calculated Osteoporosis Risk Estimation (Score) in a Sample of White Women from Belgium
Ben Sedrine, Wafa ULg; Devogelaer, J. P.; Kaufman, J. M. et al

in BONE (2001), 29(4), 374-80

Identifying patients at risk of developing an osteoporosis-related fracture will continue to be a challenge. The "gold standard" for osteoporosis diagnosis is bone densitometry. However, economic issues ... [more ▼]

Identifying patients at risk of developing an osteoporosis-related fracture will continue to be a challenge. The "gold standard" for osteoporosis diagnosis is bone densitometry. However, economic issues or availability of the technology may prevent its use under a mass screening scenario. A risk assessment instrument, the "simple calculated osteoporosis risk estimation" (SCORE), has been reported to appropriately identify women likely to have low (t score < or = -2 SD) bone mineral density (BMD) and who should be referred for bone densitometry. The aim of our study is to evaluate the discriminatory performance of SCORE in a random sample of postmenopausal white women from Belgium. For this purpose, we gathered medical data on 4035 consecutive patients aged > or = 45 years, either consulting spontaneously or referred for a BMD measurement to an outpatient osteoporosis center located at the University of Liege, Belgium. BMD measurements, using dual-energy X-ray absorptiometry (DXA) technology, were taken at the hip (total and neck) and lumbar spine (L2-4). At the recommended cutoff point of 6, SCORE had a sensitivity of 91.5% to detect low BMD at any of the measured sites, a specificity of 26.5%, a positive predictive value of 52.8%, and a negative predictive value of 77.7%. According to SCORE, 18% of the patients would not be recommended for densitometry. Among these, 10.9% were misclassified as they had osteoporosis (t score < or = -2.5 SD) at one or more of the sites investigated. The negative predictive errors of SCORE, when failing to detect osteoporosis, were only 1% for the total hip, 3.2% for the femoral neck, and 8.8% for the lumbar spine. We conclude that, notwithstanding the high values of sensitivity, SCORE specificity is too low to be useful as a diagnostic tool for screening patients at high risk to later develop osteoporosis. Nevertheless, from a resource allocation perspective, this instrument can be used with relative confidence to exclude patients who do not need a BMD measurement, and would therefore provide an opportunity to realize substantial cost savings in comparison to a mass screening strategy. [less ▲]

Detailed reference viewed: 180 (8 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the simple calculated osteoporosis risk estimation in a sample of Caucasian women from Belgium
Ben Sedrine, Wafa ULg; Devogelaer, JP; Kaufman, JM et al

in Clinical Rheumatology (2001), 20

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailEvaluation of the social impact of HPAI surveillance network at the community level in Vietnam.
Antoine-Moussiaux, Nicolas ULg; Delabouglise, Alexis; Binot, Aurélie et al

Conference (2012, August)

The need to set up efficient and sustainable surveillance networks is a major concern which must be continually placed at the heart of the overall issue of development. In developing countries, the ... [more ▼]

The need to set up efficient and sustainable surveillance networks is a major concern which must be continually placed at the heart of the overall issue of development. In developing countries, the political priority to reduce poverty means that it is vital to include social aspects in public decision making on health management in general. This focus on social aspects can be considered all the more important regarding surveillance as it is deeply embedded in agents’ everyday life. The flow of information about animal health involves different non-monetary costs, ensuing from stigmatization or from social pressure to withhold or disclose information. Understanding, measuring and alleviating these social costs of information is required to ensure the effectiveness and viability of surveillance. The present study considers the case of highly pathogenic avian influenza surveillance in Vietnam. It aims at establishing a protocol allowing for understanding and quantifying social costs incurred by surveillance agents at the community level. In this prospect, tools and concepts from anthropology, participative epidemiology and experimental economics were combined. More particularly, social network analysis, participatory observation, companion modeling and stated preference surveys were applied for the thorough examination of constraints and costs of health information flows. The opportunity for the scaling-up of such methodologies and for the inclusion of the so-elicited quantitative values in socio-economic evaluation of surveillance systems are discussed. [less ▲]

Detailed reference viewed: 74 (3 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Solitary Pulmonary Nodule by Positron Emission Tomography Imaging
BURY, Thierry ULg; Dowlati, A.; Paulus, P. et al

in European Respiratory Journal (1996), 9(3), 410-4

Current noninvasive imaging methods are not sufficiently reliable for accurate detection of malignancy in most solitary pulmonary nodules (SPNs). Positron emission tomography (PET) using 18 ... [more ▼]

Current noninvasive imaging methods are not sufficiently reliable for accurate detection of malignancy in most solitary pulmonary nodules (SPNs). Positron emission tomography (PET) using 18-fluorodeoxyglucose (FDG), showing increased FDG uptake and retention in malignant cells, has proved useful to differentiate malignant from benign tissue and could, therefore, contribute to the evaluation of the SPN. We performed a prospective study of 50 patients referred to the Pneumology Department with unclear diagnoses of SPN after conventional radiological screening. PET study was performed on each subject before an invasive procedure was proposed. Thirty three patients had a malignant nodule and 17 had a benign nodule. The mean size of malignant nodule was 3 cm (range 1.5-4.5 cm). All showed a marked increase in 18-FDG uptake. The mean size of benign nodule was 1.8 cm (range 0.5-3.5 cm). PET imaging showed the absence of 18-FDG uptake and correctly identified 15 of 17 benign nodules. There was two false-positive cases with a moderate increase in 18-FDG uptake (1 postprimary tuberculosis; and 1 anthracosilicotic nodule with nonspecific inflammation). At present, the sensitivity and specificity of the method are 100 and 88%, respectively. The positive and negative predictive values of PET imaging for SPNs are 94 and 100%, respectively. Our preliminary results demonstrate that PET-FDG imaging is a noninvasive technique, which appears highly accurate in differentiating malignant SPN from benign SPN. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Peer Reviewed
See detailEvaluation of the specific immune response against a recombinant keratinase of Microsporum canis
Vermout, S.; Brouta, F.; Descamps, F. et al

Conference (2003)

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the StrepB Select agar for the detection of group B streptococci from vaginal and recto-vaginal specimens
MELIN, Pierrette ULg; HUYNEN, Pascale ULg; MEEX, Cécile ULg et al

in ESCMID (Ed.) Program and Abstracts book of the 18th ECCMID (2008, April)

Detailed reference viewed: 60 (3 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Strepto B ID agar for the detection of group B streptococci from vaginal and recto-vaginal specimens
MELIN, Pierrette ULg; Bonafe, S.; HAYETTE, Marie-Pierre ULg et al

in ASM (Ed.) Program and Abstracts of the 106th General Meeting of the American Society for Microbiology (2006, May)

Detailed reference viewed: 36 (1 ULg)