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See detailIdentification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products
Meyer, Gilles; Bare, O.; Thiry, Etienne ULg

in Journal of General Virology (The) (1999), 80(Pt 11), 2849-59

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 ... [more ▼]

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 glycoprotein gH was sequenced. A high degree of conservation was found between BHV-1 and BHV-5 deduced gH amino acid sequences (86. 4%), which is also observed for all alphaherpesvirus gH sequences. Transcriptional analysis revealed a 3.1 kb mRNA as the specific gH transcript which was detected 2 h post-infection (p.i.). Twelve out of twenty-one MAbs directed against BHV-1 gH immunoprecipitated a 108-110 kDa glycoprotein, which was then designated BHV-5 gH. Synthesis and intracellular processing of BHV- 5 gH was analysed in infected MDBK cells using gH cross-reacting MAbs. Glycoprotein gH was expressed as a beta-gamma protein, detected by radioimmunoprecipitation as early as 3 h p.i. Glycosylation studies indicated that BHV-5 gH contains N-linked carbohydrates which are essential for the recognition of the protein by the MAbs. This suggests that N-linked glycans are involved in protein folding or are targets for the gH cross-reacting MAbs. Plaque- reduction neutralization assays showed that at least one BHV-1 gH antigenic domain is lacking in BHV-5 which may possibly relate to in vivo differences in virus tropism. [less ▲]

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See detailIdentification and characterization of four splicing variants of ovine POUF1 gene
Bastos, Estella; Avila, S.; Cravador, Alfredo et al

in Gene (2006), 382

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See detailIdentification and Characterization of Glycoprotein Gp1 of Bovine Herpesvirus Type 4
Dubuisson, J.; Pastoret, Paul-Pierre ULg; Thiry, Etienne ULg

in Journal of General Virology (The) (1992), 73((Pt 5)), 1293-6

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had ... [more ▼]

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had been removed by immunoaffinity, a fourth glycoprotein was identified. This protein (gp1) has a high Mr (greater than 300K), is detected about 8 h post-inoculation of infected cells and is strictly expressed as a gamma protein. Moreover, gp1 was identified by a polyclonal antiserum from an infected animal, indicating that this glycoprotein is an antigen recognized by the immune system of infected animals. [less ▲]

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See detailIdentification and characterization of in vivo attenuated mutants of Brucella melitensis.
Lestrate, P.; Delrue, R. M.; DANESE, Isabelle ULg et al

in Molecular Microbiology (2000), 38(3), 543-51

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within ... [more ▼]

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. Signature-tagged mutagenesis (STM) was used to identify genes required for the in vivo pathogenesis of Brucella. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5 day passage in BALB/c mice. The attenuation of 18 mutants was confirmed using an in vivo competition assay against the wild-type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone-like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of Brucella virulence. [less ▲]

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See detailIdentification and characterization of mRNAs differentially expressed in thyroid cells stimulated by a mitogenic treatment
Pirson, I.; Behrens, J.; Savonet, V. et al

in Biochimie (1999), 81

he aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A ... [more ▼]

he aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A first study has allowed to identify three new genes. Further search will use the differential display and gene arrays methodology. The role of the identified proteins coded by the genes is studied in vitro by the search of partner proteins by the double hybrid method and in vivo by mice gene knockout technology [less ▲]

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See detailIdentification and characterization of new blood-accessible colorectal cancer biomarkers
Conrotto, Paolo; Roesli, Christoph; Rybak, J. et al

Conference (2008)

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See detailIdentification and characterization of novel peptidoglycan glycosyltransferase inhibitors with antibacterial activity
Derouaux, Adeline ULg; Turk, Samo; Offant, Julien et al

Poster (2009, November)

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See detailIdentification and characterization of nuclear microsatellite loci in the aquatic moss Platyhypnidium
Hutsemekers, Virginie ULg; Risterucci, A. M.; Ricca, M. et al

in Molecular Ecology Resources (2008), 8

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See detailIdentification and culture of human bronchial epithelial cells.
Stoner, G. D.; Katoh, Y.; Foidart, Jean-Michel ULg et al

in Methods in Cell Biology (1980), 21A

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See detailIdentification and determination of alkaloids in Fumaria Species from Romania
Paltinean, Ramona; Toju, A; Wauters, Jean-Noël ULg et al

in Digest Journal of Nanomaterials & Biostructures [=DJNB] (2013), 8(2), 817-824

Four Fumaria species (F. vaillantii Loisel, F. parviflora Lam., F. rostellata Knaf and F. jankae Hausskn.) were analysed in order to determine the presence of the isoquinoline alkaloids allocryptopine ... [more ▼]

Four Fumaria species (F. vaillantii Loisel, F. parviflora Lam., F. rostellata Knaf and F. jankae Hausskn.) were analysed in order to determine the presence of the isoquinoline alkaloids allocryptopine, chelidonine, protopine, bicuculline, sanguinarine, cheleritrine, stylopine, and hydrastine through an HPLC-DAD method. Protopine and sanguinarine were present in all extracts. Bicuculline and stylopine were found in F. vaillantii and F. parviflora, whilst chelidonine was identified only in F. vaillantii and hydrastine in F. jankae, so they represent potential taxonomic markers that differentiate the four plants. The richest species in isoquinoline alkaloids was F. parviflora. Our study showed significant differences between the four Fumaria species, both qualitative and quantitative. [less ▲]

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See detailIdentification and functional characterization of a novel αlpha-conotoxin (EIIA) from Conus ermineus
Quinton, Loïc ULg; Servent, Denis; Girard, Emmanuelle et al

in Analytical and Bioanalytical Chemistry (2013), 405

Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several ... [more ▼]

Nicotinic acetylcholine receptors (nAChRs) are one of the most important families in the ligand-gated ion channel superfamily due to their involvement in primordial brain functions and in several neurodegenerative pathologies. The discovery of new ligands which can bind with high affinity and selectivity to nAChR subtypes is of prime interest in order to study these receptors and to potentially discover new drugs for treating various pathologies. Predatory cone snails of the genus Conus hunt their prey using venoms containing a large number of small, highly structured peptides called conotoxins. Conotoxins are classified in different structural families and target a large panel of receptors and ion channels. Interestingly, nAChRs represent the only subgroup for which Conus has developed seven distinct families of conotoxins. Conus venoms have thus received much attention as they could represent a potential source of selective ligands of nAChR subtypes. We describe the mass spectrometric based approaches which led to the discovery of a novel α-conotoxin targeting muscular nAChR from the venom of Conus ermineus. The presence of several posttranslational modifications complicated the N-terminal sequencing. To discriminate between the different possible sequences, analogs with variable N-terminus were synthesized and fragmented by MS/MS. Understanding the fragmentation pathways in the low m/z range appeared crucial to determine the right sequence. The biological activity of this novel α-conotoxin (α-EIIA) that belongs to the unusual α4/4 subfamily was determined by binding experiments. The results revealed not only its selectivity for the muscular nAChR, but also a clear discrimination between the two binding sites described for this receptor. [less ▲]

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See detailIdentification and interpersonal maturity : contribution to a developmental approach of juvenile delinquency
Van Poppel, E.; Born, Michel ULg

in Weitekamp, E. G. M. (Ed.) Cross-national longitudinal research on human development and criminal behavior (1994)

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See detailIdentification and localization of the structural proteins of anguillid herpesvirus 1
Van Beurden, Steven ULg; Leroy, B; Wattiez, R et al

in Veterinary Research (2011), 42(1), 105

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See detailIdentification and Measurement of Anthropogenic Effects in Landscapes
Bogaert, Jan ULg; Vranken, Isabelle ULg; Bastin, Jean-François ULg et al

in Jones, Bruce; Fu, Bojie (Eds.) The 8th World congress of the International Association of Landscape Ecology: Proceedings, Beijing, August 18-23, 2011 (2011, August 20)

As a consequence of anthropogenic pressure, landscapes change; deforestation is a well-known example of this type of human-driven landscape change. Landscapes change 42 from entirely natural to ... [more ▼]

As a consequence of anthropogenic pressure, landscapes change; deforestation is a well-known example of this type of human-driven landscape change. Landscapes change 42 from entirely natural to anthropogenic or cultural, in which landscape composition is marked by land covers and uses directly related to the civil society, such as degraded vegetations, agriculture, urban zones, or road networks. Landscape dynamics can be quantified by landscape pattern analysis. Many landscape metrics are available to capture the different features of pattern change. As a consequence of the pattern/process paradigm, the ecological consequences of the observed dynamics can be linked to ecosystem processes and characteristics, such as biodiversity. By means of a series of case studies linking field observations of fauna and flora with cartographic and demographic data, the importance of pattern analysis and landscape management is underlined, as well as the diversity in types of anthropogenic landscape change. Conclusions are drawn with regard to the ecological impact of landscape change. Guidelines are formulated for the characterization and analysis of anthropogenic effects in landscapes by means of pattern analysis. [less ▲]

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See detailIdentification and model Updating of a non-stationary vibrating system
Dimitriadis, Grigorios ULg; Fassois, Spilios; Poulimenos, Aggelos et al

in Akay, A.; Arnas, O.; Cooper, J. E. (Eds.) et al Proceedings of 7th Biennial ASME Conference on Engineering Systems Design and Analysis (2004, July)

Non-stationary systems, which are commonly encountered in many fields of science, are characterized by time-varying features and require time-frequency methods for their analysis. This study considers the ... [more ▼]

Non-stationary systems, which are commonly encountered in many fields of science, are characterized by time-varying features and require time-frequency methods for their analysis. This study considers the problem of identification and model updating of a non-stationary vibrating system. In particular, a number of identification methods and a model updating procedure are evaluated and compared through application to a time-varying “bridge-like” laboratory structure. The identification approaches include Frequency Response Function based parameter estimation techniques, Subspace Identification and Functional Series modelling. All methods are applied to both output-only and input-out-put data. Model updating is based upon a theoretical model of the structure obtained using a Rayleigh-Ritz methodology, which is updated to account for time-dependence and nonlinearity via the identification results. Interesting comparisons, among both identification and model updating results, are performed. The results of the study demonstrate high modelling accuracy, illustrating the effectiveness of model updating techniques in non-stationary vibration modelling. [less ▲]

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See detailIdentification and modulation of a growth hormone-binding protein in rainbow trout (Oncorhynchus mykiss) plasma during seawater adaptation.
Sohm, F.; Manfroid, Isabelle ULg; Pezet, A. et al

in General and Comparative Endocrinology (1998), 111(2), 216-24

A soluble protein that specifically bound 125I-human growth hormone (hGH) was identified in rainbow trout plasma, using HPLC-gel filtration. The binding affinity of the protein for hGH was 1.2 x 10(9)M-1 ... [more ▼]

A soluble protein that specifically bound 125I-human growth hormone (hGH) was identified in rainbow trout plasma, using HPLC-gel filtration. The binding affinity of the protein for hGH was 1.2 x 10(9)M-1. 125I-rainbow trout GH (tGH) was also able to bind to the protein albeit with a lower affinity (6.6 x 10(7)M-1) than hGH. Crosslinking experiments using 125I-hGH revealed two specific bands of 150 and 130 kDa. The complex 125I-hGH-BP could be precipitated by a monoclonal anti-GH receptor antibody, suggesting a close relationship between the plasma GH-BP and the GH receptor. A fourfold increase in the hGH binding to the GH-BP was shown 48 h after transfer of the fishes from freshwater to seawater. The increase in binding was related to a high binding capacity without significant changes in binding affinity. These results suggest a potential role of this related GH-BP as an index of GH effects during seawater adaptation in salmonids. [less ▲]

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See detailIdentification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1
Basu, Joyoti; Mahapatra, Sebabrata; Kundu, Manikuntala et al

in Journal of Bacteriology (1996), 178(6), 1707-1711

Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high ... [more ▼]

Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities. When produced in E. coli, His tag-labelled derivatives of M. leprae PBP1 adopt the correct membrane topology, with the bulk of the polypeptide chain on the surface of the plasma membrane. They defy attempts at solubilization with all the detergents tested except cetyltrimethylammonium bromide. The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins. [less ▲]

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See detailIdentification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics
Collodoro, Mike ULg; Lemaire, Pascale ULg; Eppe, Gauthier ULg et al

in Journal of Proteomics (2012), in press

This paper reports the identification of biomarkers resulting from the exposure of MCF-7/BOS cells to 17β-estradiol (E2). The biomarkers were identified using 2 independent and complementary techniques, 2 ... [more ▼]

This paper reports the identification of biomarkers resulting from the exposure of MCF-7/BOS cells to 17β-estradiol (E2). The biomarkers were identified using 2 independent and complementary techniques, 2-D DIGE / MALDI-TOF peptide mass fingerprint, and 2-D UPLC-ESI MS/MS. These markers form a preliminary molecular signature that can be used when testing the estrogenic activity of xenobiotics, either pure or in mixtures. [less ▲]

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