Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
See detailEx iusta causa traditum: Essays in Honour of Eric H. Pool
Van den Bergh, Rena; van Niekerk, Gardiol; Thomas, Philip et al

Book published by UNISA (2005)

Detailed reference viewed: 144 (0 ULg)
Peer Reviewed
See detailEx vivo bioluminescent detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever induced in rabbits
Dewals, Benjamin G ULg; Myster, Françoise ULg; Massart, François et al

Poster (2009, December 11)

Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be reproduced in rabbits. The lesions observed are very similar to those described in natural host species. Recently, we demonstrated that WD-MCF induced by AlHV-1 in rabbits is associated with the proliferation of CD8+ cells supporting a latent type of infection. In the present study, we investigated whether the virus could be detected ex vivo in the tissues of rabbits developing WD-MCF. Taking advantage of the recent cloning of the AlHV-1 genome as a bacterial artificial chromosome (BAC), we produced a recombinant plasmid encoding a firefly luciferase (LUC) expression cassette inserted in a non-coding region of the AlHV-1 genome. In vitro, the reconstituted AlHV-1 LUC strain replicated comparably to the parental strain in permissive cells and was able to induce a bioluminescent signal. In vivo, rabbits infected with the AlHV-1 LUC strain developed WD-MCF similarly to the parental wild-type strain with hyperthermia, increased CD8/CD4 ratio and viral genomic charge over time in PBMC and in lymph nodes at time of death. To identify the presence of AlHV-1 infection ex vivo, various organs of infected rabbits developing WD-MCF were analysed by bioluminescent imaging. Luciferase activity could be detected macroscopically at the time of death in most of analyzed organs including lung, popliteal and mesenteric lymph nodes, spleen, liver, kidney and appendix. Infectious virus could be isolated following co-cultures of lymph node and permissive cells, and the isolated virus retained the ability to induce a bioluminescent signal. In conclusion, we produced an AlHV-1 LUC recombinant and we were able to detect the AlHV-1 infection ex vivo in many organs at the time of death. [less ▲]

Detailed reference viewed: 55 (25 ULg)
Full Text
Peer Reviewed
See detailEx vivo bioluminescent detection of Alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
Dewals, Benjamin G ULg; Myster, Françoise ULg; Palmeira, Leonor ULg et al

in Journal of Virology (2011), 85(14), 6941-54

Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV-1), carried by wildebeest asymptomatically, causes malignant catarrhal fever (WD-MCF) when cross-species transmitted to a variety of susceptible species of the Artiodactyla order. Experimentally, WD-MCF can be reproduced in rabbits. WD-MCF is described as a combination of lymphoproliferation and degenerative lesions in virtually all organs and caused by unknown mechanisms. Recently, we demonstrated that WD-MCF is associated with the proliferation of CD8(+) cells supporting a latent type of infection in lymphoid tissues. Here, we investigated the macroscopic distribution of AlHV-1 infection using ex vivo bioluminescence imaging in rabbit to determine whether it correlates with the distribution of lesions in lymphoid and non-lymphoid organs. To reach that goal, a recombinant AlHV-1 strain was produced by insertion of a luciferase expression cassette (luc) in an intergenic region. In vitro, the reconstituted AlHV-1 luc(+) strain replicated comparably to the parental strain and luciferase activity was detected by bioluminescence imaging. In vivo, rabbits infected with the AlHV-1 luc(+) strain developed WD-MCF comparably to the parental wild-type strain with hyperthermia and increase of both CD8(+) T cells frequencies and viral genomic charge over time in peripheral blood mononuclear cells and in lymph nodes at time of euthanasia. Bioluminescent imaging revealed that AlHV-1 infection could be detected ex vivo in lymphoid organs but also in lung, liver and kidney during WD-MCF, demonstrating that AlHV-1 infection is prevalent in tissue lesions. Finally, we show that the infiltrating mononuclear leukocytes in non-lymphoid organs are mainly CD8(+) T cells and that latency is predominant during WD-MCF. [less ▲]

Detailed reference viewed: 88 (32 ULg)
Full Text
Peer Reviewed
See detailEx Vivo Evaluation of p-[18F]D-MPPF for the Study of 5-HT1A Receptors.
Defraiteur, C.; Lemaire, Christian ULg; Luxen, André ULg et al

in Journal of Labelled Compounds & Radiopharmaceuticals (2005), 48

Detailed reference viewed: 15 (5 ULg)
Full Text
Peer Reviewed
See detailEx vivo expansion of hematopoietic progenitor cells is associated with downregulation of alpha4 integrin- and CXCR4-mediated engraftment in NOD/SCID beta2-microglobulin-null mice
Foguenne, Jacques ULg; Di Stefano; Giet, Olivier ULg et al

in Haematologica (2009), 94

Several studies indicate that ex vivo cytokine-supported expansion induces defective hematopoietic stem cell engraftment. We investigated the role of alpha4 integrin, alpha5 integrin and CXCR4 in ... [more ▼]

Several studies indicate that ex vivo cytokine-supported expansion induces defective hematopoietic stem cell engraftment. We investigated the role of alpha4 integrin, alpha5 integrin and CXCR4 in engraftment of unmanipulated and cytokine-treated human cord blood CD34+ cells. [less ▲]

Detailed reference viewed: 41 (18 ULg)
Full Text
Peer Reviewed
See detailEx vivo ruminal cellulosome for by-product biomass conversion
Hissette, Mathias ULg; Destain, Jacqueline ULg; Thewis, André ULg et al

Conference (2011, April 27)

Detailed reference viewed: 54 (16 ULg)
Full Text
Peer Reviewed
See detail(Ex)communication
Dumortier, Jean-Louis ULg

in Lettre de la DLFM (2003), 32

Detailed reference viewed: 8 (2 ULg)
Full Text
Peer Reviewed
See detail(Ex-)smoking asthma patients in general and specialized Belgian practice.
Decramer, M.; Louis, Renaud ULg; Joos, G. et al

in Respiratory medicine (2011), 105(8), 1203-1210

INTRODUCTION: Smokers are often excluded from asthma studies. In the present study, data are presented on the prevalence, characteristics and management approach of this patient population in the Belgian ... [more ▼]

INTRODUCTION: Smokers are often excluded from asthma studies. In the present study, data are presented on the prevalence, characteristics and management approach of this patient population in the Belgian practice both at the level of general practitioners (GPs) and specialists. MATERIALS AND METHODS: One hundred and nineteen smoking, non-smoking and ex-smoking patients (25-65 yrs) with asthma, COPD or both, were recruited by 33 GPs and 33 specialists. Data were obtained retrospectively from medical records. However, only a small number of files were complete. RESULTS: The majority of COPD patients were (ex-)smokers: 94% in the specialist group, 78% in the GP group. Cardiovascular comorbidity appeared in both groups in the same frequency order: COPD>(ex-)smoking patients with asthma (AS)>non-smoking patients with asthma (ANS), with a significant difference between AS and ANS in the specialist population. Chronic cough during more than 3 months in two consecutive years was reported in 97% of COPD patients, in 71% of the AS patients and in only 25% of the ANS patients. The type of cough differed between AS and ANS in the GP group, with a higher prevalence of productive cough in the former. Treatment patterns observed were as expected according to diagnosis except for a disproportionate use of Tiotropium in AS in the GP group. CONCLUSION: AS were somewhere in between COPD patients and ANS for a large number of the characteristics studied, suggesting that they are an intermediate phenotype between COPD and asthma. [less ▲]

Detailed reference viewed: 34 (17 ULg)
See detailEx-situ’ conservation of Antarctic cyanobacteria : a culture collection to explore diversity and bioactivity
Wilmotte, Annick ULg; Renard, Marine ULg; Kleinteich, J et al

Poster (2014, August)

Cyanobacteria appear as the dominant phototrophs in Antarctic terrestrial and freshwater ecosystems. Since 2011, the Belgian Science Policy Office has funded the BCCM/ULC public collection of (sub)polar ... [more ▼]

Cyanobacteria appear as the dominant phototrophs in Antarctic terrestrial and freshwater ecosystems. Since 2011, the Belgian Science Policy Office has funded the BCCM/ULC public collection of (sub)polar cyanobacteria. It is currently holding 102 Antarctic cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. A Quality Management System was implemented and an ISO9001 certificate was obtained for the public deposition and distribution of strains. The strains are kept as living cultures, and their cryopreservation is in progress. The Antarctic cyanobacterial strains were isolated from samples of the three main biogeographic provinces. The purpose of this public collection is to gather a representative portion of the cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the diversity, evolution, adaptations to the environmental conditions, and genomic make-up. Three cyanobacterial orders are represented: Chroococcales, Oscillatoriales and Nostocales. This is particularly important in view of the emerging use of metagenomic approaches on environmental samples, where the comparisons with the genome sequences from well-defined strains is very useful. They could also serve as references for compounds such as fatty acids and pigments. In addition, cyanobacteria produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with different bioactive potential (e.g. antibiotic, antiviral, anticancer, cytotoxic, genotoxic). Bioassays have shown antifungal activities of the cell extracts of strains Plectolyngbya hodgsonii ULC009 and Phormidium priestleyi ULC026. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems especially promising for biotechnology or biomedical applications. In summary, the BCCM/ULC public collection could serve as a general reference for Antarctic cyanobacteria with multiple applications, as well as a resource for novel bioactive compounds. [less ▲]

Detailed reference viewed: 62 (1 ULg)
See detail‘Ex-situ’ preservation and characterization of Antarctic cyanobacteria in the BCCM/ULC collection
Kleinteich, Julia ULg; Renard, Marine ULg; Simons, Véronique et al

Poster (2015, June 09)

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. An ISO9001 certificate was obtained for the public deposition and distribution of ... [more ▼]

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. An ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. BCCM/ULC is currently holding 160 public cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/catalogues/ulc-catalogue-search. Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. The main holding of the collection concerns (sub)polar strains isolated from different biotopes and representative of a large taxonomic diversity. The molecular characterization is underway, on the basis of 16S rRNA and ITS sequences, but also Multiple Locus Sequence Analysis and genome sequencing. In addition, cyanobacteria are known to produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with various bioactive potential. The presence of genes involved in the production of microcystin is currently studied by PCR, and analytical methods are used to confirm the toxin production. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems especially promising for biotechnology or biomedical applications. [less ▲]

Detailed reference viewed: 55 (3 ULg)
Peer Reviewed
See detailEx-vivo cytokine production by whole blood cells from cancer patients
De Groote, Donat; Vrindts-Gevaert, Yvonne; Lopez Y Cadenas, Miguel ULg et al

in Cancer Detection & Prevention (1996), 20

Detailed reference viewed: 24 (6 ULg)
Full Text
Peer Reviewed
See detailLes exacerbations dans la BPCO: un fardeau a reduire
Corhay, Jean-Louis ULg; Nguyen Dang, Delphine ULg; Louis, Renaud ULg

in Revue Médicale de Liège (2006), 61(10), 691-6

Exacerbations of chronic obstructive pulmonary disease (COPD) play a very important role. Unfortunately they were neglected a long time in the therapeutic tests. However exacerbations influence the ... [more ▼]

Exacerbations of chronic obstructive pulmonary disease (COPD) play a very important role. Unfortunately they were neglected a long time in the therapeutic tests. However exacerbations influence the decline of the respiratory function over time, cause important deterioration of the quality of life of the patients, increase morbidity and mortality of COPD, and finally represent a burden for health care. Reducing the number of exacerbations could potentially slow down the progression of the disease. Thus the prevention of exacerbations should be the "corner stone" of the maintenance treatment of the COPD. In this review we propose to recall the importance of exacerbations in COPD and to present the treatment which have been shown to reduce exacerbation rate in COPD. [less ▲]

Detailed reference viewed: 164 (9 ULg)
Full Text
Peer Reviewed
See detailExact and Heuristic Solution Methods for a VRP with Time Windows and Variable Service Start Time
Michelini, Stefano ULg; Arda, Yasemin ULg; Crama, Yves ULg et al

Conference (2015, June 10)

We consider a VRP with time windows in which the total cost of a solu- tion depends on the total duration of the vehicle routes, and the starting time for each vehicle is a decision variable. We first ... [more ▼]

We consider a VRP with time windows in which the total cost of a solu- tion depends on the total duration of the vehicle routes, and the starting time for each vehicle is a decision variable. We first develop a Branch- and-Price (BP) algorithm considering the related pricing subproblem, an elementary shortest path problem with resource constraints (ESP- PRC). We discuss past, present and planned research on this exact so- lution methodology, based on a bidirectional dynamic programming approach for the ESPPRC, and on the design of a matheuristic. [less ▲]

Detailed reference viewed: 23 (5 ULg)
Full Text
Peer Reviewed
See detailExact and Heuristic Solution Methods for a VRP with Time Windows and Variable Service Start Time
Michelini, Stefano ULg; Arda, Yasemin ULg; Crama, Yves ULg et al

Conference (2015, February 05)

We consider a VRP with time windows in which the total cost of a solution depends on the duration of the vehicle routes, and the starting time for each vehicle is a decision variable. We first develop a ... [more ▼]

We consider a VRP with time windows in which the total cost of a solution depends on the duration of the vehicle routes, and the starting time for each vehicle is a decision variable. We first develop a Branch-and-Price algorithm considering the pricing subproblem, an elementary shortest path problem with resource constraints (ESPPRC). We discuss research on this exact solution methodology, based on a bidirectional dynamic programming approach for the ESPPRC, and on the design of a matheuristic. [less ▲]

Detailed reference viewed: 73 (22 ULg)
Peer Reviewed
See detailAn exact bi-directional dynamic programming algorithm for an elementary shortest path problem with variable service start time
Arda, Yasemin ULg; Crama, Yves ULg; Kucukaydin, Hande ULg

Conference (2014, January 31)

We consider an elementary shortest path problem with resource constraints (ESPPRC), where a capacitated single vehicle serves customers by respecting their associated time windows. The vehicle can start ... [more ▼]

We consider an elementary shortest path problem with resource constraints (ESPPRC), where a capacitated single vehicle serves customers by respecting their associated time windows. The vehicle can start servicing the customers at any desired time, but it can be used for a fixed amount of time. The total transportation cost depends on the total distance traveled and the total amount of time that the vehicle spends by performing the assigned trip. On the other hand, each served customer yields a revenue. Thus, the aim is to identify the path to be followed and the start time of the vehicle from the depot that minimize the total transportation cost minus the gained revenues. This kind of a problem can be encountered as a pricing subproblem when a branch-and-price algorithm is applied to solve vehicle routing problems with additional constraints. In such a case, the revenues correspond to the dual prices of the visited vertices. It is known that the classical ESPPRC can be solved to optimality by implementing a dynamic programming (DP) algorithm. However, our problem has to take an infinite number of Pareto-optimal states into consideration, since the vehicle can leave the depot at any point in time and charges depending on the total traveling and waiting time. We propose an exact DP algorithm which can deal with an infinite number of Pareto-optimal states by representing total traveling and waiting time as a piecewise linear function of the service start time at the depot and develop suitable dominance rules. Furthermore, a column generation algorithm is devised for solving the relaxed set covering formulation of the related vehicle routing problem where new columns are determined by the proposed DP algorithm. Finally, computational results are presented. [less ▲]

Detailed reference viewed: 142 (1 ULg)
Full Text
Peer Reviewed
See detailAn Exact Method for Designing Shewhart X ̅ and S2 Control Charts to Guarantee the In-Control Performance
Faraz, Alireza ULg; Heuchenne, Cédric ULg

in Journal of Quality Technology (2016)

The in-control performance of the Shewhart X ̅ and S2 control charts with estimated in-control parameters has been evaluated by a number of authors. Results indicate an unrealistically large amount of ... [more ▼]

The in-control performance of the Shewhart X ̅ and S2 control charts with estimated in-control parameters has been evaluated by a number of authors. Results indicate an unrealistically large amount of Phase I data is needed to have the desired in-control average run length (ARL) value in Phase II. To overcome this problem, it has been recommended that the control limits be adjusted based on a bootstrap method to guarantee that the in-control ARL is at least a specified value with a certain specified probability. In our paper we present simple formulas for the required control limits so that practitioners do not have to use the bootstrap method. An assumption of normality is required. The advantage of our proposed method is in its simplicity; there is no bootstrapping and the control chart constants do not depend on the Phase I sample data. [less ▲]

Detailed reference viewed: 49 (2 ULg)
Full Text
Peer Reviewed
See detailExact Model Reduction of Combinatorial Reaction Networks
Conzelmann, Holger; Fey, Dirk ULg; Gilles, E. D.

in BMC Systems Biology (2008), 2

Detailed reference viewed: 20 (12 ULg)