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See detailHuman TEF-5 is preferentially expressed in placenta and binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer
Jacquemin, Patrick; Martial, Joseph ULg; Davidson, Irwin

in Journal of Biological Chemistry (1997), 272(20), 12928-37

We report the cloning of a cDNA encoding the human transcription factor hTEF-5, containing the TEA/ATTS DNA binding domain and related to the TEF family of transcription factors. hTEF-5 is expressed in ... [more ▼]

We report the cloning of a cDNA encoding the human transcription factor hTEF-5, containing the TEA/ATTS DNA binding domain and related to the TEF family of transcription factors. hTEF-5 is expressed in skeletal and cardiac muscle, but the strongest expression is observed in the placenta and in placenta-derived JEG-3 choriocarcinoma cells. In correlation with its placental expression, we show that hTEF-5 binds to several functional enhansons of the human chorionic somatomammotropin (hCS)-B gene enhancer. We define a novel functional element in this enhancer comprising tandemly repeated sites to which hTEF-5 binds cooperatively. In the corresponding region of the hCS-A enhancer, which is known to be inactive, this element is inactivated by a naturally occurring single base mutation that disrupts hTEF-5 binding. We further show that the binding of the previously described placental protein f/chorionic somatomammotropin enhancer factor-1 to TEF-binding sites is disrupted by monoclonal antibodies directed against the TEA domain and that this factor is a proteolytic degradation product of the TEF factors. These results strongly suggest that hTEF-5 regulates the activity of the hCS-B gene enhancer. [less ▲]

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See detailHuman thymic epithelial cells are potential targets for the diabetogenic coxsackievirus B4
Brilot, Fabienne; Hober, Didier; Geenen, Vincent ULg

Poster (2000, July)

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See detailHuman TUBB3 mutations perturb microtubule dynamics, kinesin interactions, and axon guidance.
Tischfield, Max A; Baris, Hagit N; Wu, Chen et al

in Cell (2010), 140(1), 74-87

We report that eight heterozygous missense mutations in TUBB3, encoding the neuron-specific beta-tubulin isotype III, result in a spectrum of human nervous system disorders that we now call the TUBB3 ... [more ▼]

We report that eight heterozygous missense mutations in TUBB3, encoding the neuron-specific beta-tubulin isotype III, result in a spectrum of human nervous system disorders that we now call the TUBB3 syndromes. Each mutation causes the ocular motility disorder CFEOM3, whereas some also result in intellectual and behavioral impairments, facial paralysis, and/or later-onset axonal sensorimotor polyneuropathy. Neuroimaging reveals a spectrum of abnormalities including hypoplasia of oculomotor nerves and dysgenesis of the corpus callosum, anterior commissure, and corticospinal tracts. A knock-in disease mouse model reveals axon guidance defects without evidence of cortical cell migration abnormalities. We show that the disease-associated mutations can impair tubulin heterodimer formation in vitro, although folded mutant heterodimers can still polymerize into microtubules. Modeling each mutation in yeast tubulin demonstrates that all alter dynamic instability whereas a subset disrupts the interaction of microtubules with kinesin motors. These findings demonstrate that normal TUBB3 is required for axon guidance and maintenance in mammals. [less ▲]

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See detailThe human tumor-associated antigen RCAS1 in pregnancies complicated by pre-eclampsia.
Tskitishvili, Ekaterine ULg; Komoto, Yoshiko; Kinugasa, Yukiko et al

in Journal of Reproductive Immunology (2008), 77(1), 100-8

The human tumor-associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is considered to play a role in the escape of tumor cells from immune surveillance and, at the same time ... [more ▼]

The human tumor-associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is considered to play a role in the escape of tumor cells from immune surveillance and, at the same time, participates in the inhibition of the maternal immune response during pregnancy. The aim of our study was to investigate the expression of tumor-associated RCAS1 protein in the placenta and amniotic membranes and to assess and compare its concentration in amniotic fluid, maternal and cord blood sera in pregnancies complicated by pre-eclampsia. Samples were obtained from women with pre-eclampsia (N=9), pre-eclampsia with IUGR (N=4), normotensive IUGR (N=7) and healthy term controls (N=25) after delivery. Placentas were studied by immunohistochemistry, Western blot analysis and real-time (RT)-PCR. For assessment of RCAS1 protein concentrations in biological fluids, ELISA was performed. RCAS1 mRNA expression in the placentas of pre-eclamptic patients was significantly lower than in controls (p<0.01). The maternal blood serum RCAS1 protein concentration in the pre-eclampsia cases was also significantly lower than in controls (p=0.0207). The other study groups did not differ significantly. This study reveals the possible role of the RCAS1 protein in the development of pre-eclampsia through an immunological pathway. [less ▲]

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See detailHuman viral infections derived from food
Scipioni, Alexandra ULg; Daube, Georges ULg; Thiry, Etienne ULg

in Point Vétérinaire (2001), 32(218, AUG-SEP),

Several viruses may be transmitted to humans via food including hepatitis A and E virus (VHA and VHE), Norwalk type calicivirus, rotavirus and astrovirus. Enterovirus and enteric adenovirus may also be ... [more ▼]

Several viruses may be transmitted to humans via food including hepatitis A and E virus (VHA and VHE), Norwalk type calicivirus, rotavirus and astrovirus. Enterovirus and enteric adenovirus may also be transmitted. Sick people or healthy carriers excrete these viruses in their stools. Shellfish and water are the most frequently contaminated foodstuffs. Another route of infection is during handling of food. Detection of these viruses in food is complex for several reasons: the interaction of virus and food makes viral concentration and purification difficult, in vitro culture of these viruses is difficult if not impossible, and the quantity of virus present in the sample is low. Molecular technology (PCR) is the most suitable method of detection. Another method is to choose an indicator of viral faecal contamination. [less ▲]

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See detailThe Human Vpac(1) Receptor - Three-Dimensional Model And Mutagenesis Of The N-Terminal Domain
Lins, Laurence ULg; Couvineau, A.; Rouyer-Fessard, C. et al

in Journal of Biological Chemistry (2001), 276(13),

The human VPAC(1) receptor for vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating peptide belongs to the class II family of G-protein-coupled receptors with seven transmembrane ... [more ▼]

The human VPAC(1) receptor for vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating peptide belongs to the class II family of G-protein-coupled receptors with seven transmembrane segments. Like for all class II receptors, the extracellular N-terminal domain of the human VPAC(1) receptor plays a predominant role in peptide ligand recognition. To determine the three-dimensional structure of this N-terminal domain (residues 1-144), the Protein Data Bank (PDB) was screened for a homologous protein. A subdomain of yeast lipase B was found to have 27% sequence identity and 50% sequence homology with the N-terminal domain (8) of the VPAC(1) receptor together with a good alignment of the hydrophobic clusters. A model of the N-terminal domain of VPAC(1) receptor was thus constructed by homology. It indicated the presence of a putative signal sequence in the N-terminal extremity. Moreover, residues (Glu(36), Trp(67), Asp(68), Trp(73), and Gly(109)) which were shown to be crucial for VIP binding are gathered around a groove that is essentially negatively charged. New putatively important residues for VIP binding were suggested from the model analysis. Site-directed mutagenesis and stable transfection of mutants in CHO cells indicated that Pro(74), Pro(87), Phe(90), and Trp(110) are indeed important for VIP binding and activation of adenylyl cyclase activation. Combination of molecular modeling and directed mutagenesis provided the first partial three-dimensional structure of a VIP-binding domain, constituted of an electronegative groove with an outspanning tryptophan shell at one end, in the N-terminal extracellular region of the human VPAC(1) receptor. [less ▲]

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See detailHuman xeroderman group G gene encodes a DNA endonuclease
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Nucleic Acids Research (1994), 22(16), 3312-6

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See detailThe human-macaque interface: the impact of anthropogenic factors on the behavioural ecology of Macaca fascicularis in Bangkok (Thailand) and Bali (Indonesia)
Brotcorne, Fany ULg; Huynen, Marie-Claude ULg; Wandia, I Nengah

Conference (2010, October 11)

The human-macaque interface is increasingly the focus of many researches in East and South-east Asia. The efforts target the understanding of human impacts on macaques and the risks of pathogen ... [more ▼]

The human-macaque interface is increasingly the focus of many researches in East and South-east Asia. The efforts target the understanding of human impacts on macaques and the risks of pathogen transmission, the mitigation of conflicts as well as the establishment of suitable management programs. Commensal relationships between humans and several species of macaques exist for centuries in some Asian locations, like in Bali (Indonesia), Japan or India. However, the frequency and the intensity of these interactions are strongly increasing over the recent decades, due to the extensive urbanization and recruitment of forestlands for cropping. We conducted a comparative study on two commensal long-tailed macaque (Macaca fascicularis) populations, one in Bangkok (Thailand) and the other in Bali (Indonesia), in order to assess the impact of anthropogenic factors (human presence and food provisioning frequency) on some eco-behavioural aspects of this macaque species living in urban landscapes. We found a consistent impact on the activity budget and the diet composition in both populations, suggesting an important role played by these anthropogenic factors for this species. Additionally, we did not find any impact of food provisioning on agonistic interactions in the two populations, contrary to previous studies in other macaque species. We suggest this surprising result could be explained by the high abundance and constant availability of human food in both sites, decreasing foraging pressures and the associated social competition. Further field studies are in progress in other populations of Macaca fascicularis in Bali in order to confirm this consistency of human impact on the behavioural ecology [less ▲]

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See detailHuman-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication.
Cherrier, Thomas ULg; Elias, Mikael; Jeudy, Alicia et al

in Virology Journal (2011), 8

The Human Phosphate-Binding protein (HPBP) is a serendipitously discovered lipoprotein that binds phosphate with high affinity. HPBP belongs to the DING protein family, involved in various biological ... [more ▼]

The Human Phosphate-Binding protein (HPBP) is a serendipitously discovered lipoprotein that binds phosphate with high affinity. HPBP belongs to the DING protein family, involved in various biological processes like cell cycle regulation. We report that HPBP inhibits HIV-1 gene transcription and replication in T cell line, primary peripherical blood lymphocytes and primary macrophages. We show that HPBP is efficient in naive and HIV-1 AZT-resistant strains. Our results revealed HPBP as a new and potent anti HIV molecule that inhibits transcription of the virus, which has not yet been targeted by HAART and therefore opens new strategies in the treatment of HIV infection. [less ▲]

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See detailHuman-Visual-System-Based Fusion of Multimodality 3D Neuroimagery using Brain-Shift-Compensating Finite-Element-Based Deformable Models
Verly, Jacques ULg; Vigneron, Lara; Petitjean, Nicolas et al

in Proceedings of SPIE (2003, February), 5029

Our goal is to fuse multimodality imagery to enhance image-guided neurosurgery. Images that need to be fused must be registered. Registration becomes a challenge when the imaged object deforms between the ... [more ▼]

Our goal is to fuse multimodality imagery to enhance image-guided neurosurgery. Images that need to be fused must be registered. Registration becomes a challenge when the imaged object deforms between the times the images to be fused are taken. This is the case when “brain-shift” occurs. We begin by describing our strategy for nonrigid registration via finite-element methods. Then, we independently discuss an image fusion strategy based on a model of the human visual system. We illustrate the operation of many components of the registration system and the operation of the fusion system. [less ▲]

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See detailHuman/bovine chimeric MxA-like GTPases reveal a contribution of N-terminal domains to the magnitude of anti-influenza A activity
Garigliany, Mutien-Marie ULg; Cornet, Anne ULg; Desmecht, Daniel ULg

in Journal of Interferon & Cytokine Research (2012), 32(7), 326-331

Type I interferons (IFN-) provide powerful and universal innate intracellular defence mechanisms against viruses. Among the antiviral effectors induced by IFN-, Mx proteins of some species appear as ... [more ▼]

Type I interferons (IFN-) provide powerful and universal innate intracellular defence mechanisms against viruses. Among the antiviral effectors induced by IFN-, Mx proteins of some species appear as key components of defence against influenza A viruses. The body of work published to date suggests that to exert anti-influenza activity, an Mx protein must possess a GTP-binding site, structural bases allowing multimerisation, and a specific C-terminal GTPase effector domain (GED). The human MxA and bovine Mx1 proteins both meet these minimal requirements, but the bovine protein is more active against influenza viruses. Here we measured the anti-influenza activity exerted by two human/bovine chimeric Mx proteins. We show that substituting the bovine GED for the human one in human MxA does not affect the magnitude of anti-influenza activity. Strikingly, however, substituting the human GED for the bovine one in bovine Mx1 yields a chimeric protein with much higher anti-influenza activity than the human protein. We conclude, in contradiction to the hypothesis currently in vogue in the literature, that the GED is not the sole determinant controlling the magnitude of the anti-influenza activity exercised by an Mx protein that can bind GTP and multimerise. Our results suggest that one or several motifs that remain to be discovered, located N-terminally with respect to the GED, may interact with a viral component or a cellular factor so as to alter the viral cycle. Identifying, in the N-terminal portion of bovine Mx1, the motif(s) responsible for its higher anti-influenza activity could contribute to the development of new anti-influenza molecules. [less ▲]

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See detailHumanisation de la marque et web 2.0
Brandt, Céline ULg

Article for general public (2012)

La personnification est l’action de représenter sous les traits d’une personne une chose abstraite ou inanimée. Au sein des réseaux sociaux, les consommateurs souhaitent s’adresser directement au porte ... [more ▼]

La personnification est l’action de représenter sous les traits d’une personne une chose abstraite ou inanimée. Au sein des réseaux sociaux, les consommateurs souhaitent s’adresser directement au porte-parole de la marque, en général le dirigeant de la société. Les conséquences de ce type de communication personnelle sur le comportement du consommateur sont l’amélioration de la confiance, la satisfaction et l’engagement du consommateur. [less ▲]

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See detailL'humanisation de la mort
Lamy, Maurice ULg

in Annales de l'Anesthésiologie Française (1980), 21(3), 313-314

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See detailL'humanisme face au terrorisme
Seron, Vincent ULg

in Cahiers de défense sociale (2000)

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See detailHumanisme, classicisme et tradition dans la vie et l’œuvre d’Arsène Soreil
Delhalle, Nancy ULg

in La Vie Wallonne (1989), 405-406

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See detailLes humanités chrétiennes
Kurth, Godefroid ULg

Speech (1901)

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See detailLes humanités de demain
Kurth, Godefroid ULg

in Bulletin de la Classe des Lettres et des Sciences Morales et Politiques (1902)

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See detailHumanities 2.0. Document, interpretation and intersubjectivity in the digital age
Bénel, Aurélien; Lejeune, Christophe ULg

in International Journal of Web-Based Communities (2009), 5(4), 562-576

With their focus on documents, interpretation and intersubjectivity, Web 2.0 technologies have surprising analogies with philosophical hermeneutics, the theory of texts interpretation. Philosophical ... [more ▼]

With their focus on documents, interpretation and intersubjectivity, Web 2.0 technologies have surprising analogies with philosophical hermeneutics, the theory of texts interpretation. Philosophical hermeneutics was generalised from biblical hermeneutics by Dilthey in the 19th century, and chosen as an alternative to positivism as a foundation for the epistemology and methodology of the humanities and social sciences. This article explores how Web 2.0 technologies might better meet the needs of social and human sciences than traditional information technologies that are historically bound with logical positivism. Illustrations are provided from archaeology and sociology, two social and human sciences which were early adopters of punched cards and computers. [less ▲]

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See detailHumanizing the Clinical Gaze: Movies and the Empathic Understanding of Psychosis
Raballo, A.; Laroi, Frank ULg; Bell, V.

in Family Medicine (2009), 41(6), 387-388

Detailed reference viewed: 7 (1 ULg)