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See detailEvaluation of BM-573, a novel TXA(2) synthase inhibitor and receptor antagonist, in a porcine model of myocardial ischemia-reperfusion
Kolh, Philippe ULg; Rolin, S.; Tchana-Sato, Vincent ULg et al

in Prostaglandins & Other Lipid Mediators (2006), 79(1-2), 53-73

Aims: To investigate whether BM-573 (N-tert-butyl-N'-[2-(4'-methylphenylam\ino)-5-nitro-benzenesulfonyl]urea), an original combined thromboxane A(2) synthase inhibitor and receptor antagonist, prevents ... [more ▼]

Aims: To investigate whether BM-573 (N-tert-butyl-N'-[2-(4'-methylphenylam\ino)-5-nitro-benzenesulfonyl]urea), an original combined thromboxane A(2) synthase inhibitor and receptor antagonist, prevents reperfusion injury in acutely ischemic pigs. Methods: Twelve animals were randomly divided in two groups: a control group (n = 6) intravenously infused with vehicle, and a BM-573-treated group (n = 6) infused with BM-573 (10 mg kg(-1) h(-1)). In both groups, the left anterior descending (LAD) coronary artery was Occluded for 60 min and reperfused for 240 min. Either vehicle or BM-573 was infused 30 min before LAD occlusion and throughout the experiment. Platelet aggregation induced by arachidonic acid ex vivo measured was prevented by BM-573. Results: In both groups, LAD occlusion decreased cardiac output, ejection fraction, slope of stroke work-end-diastolic volume relationship, and induced end-systolic pressure-volume relationship (ESPVR) rightward shift, while left ventricular afterload increased. Ventriculo-arterial coupling and mechanical efficiency decreased. In both groups, reperfusion further decreased cardiac output and ejection fraction, while ESPVR displayed a further rightward shift. Ventriculo-arterial coupling and mechanical efficiency remained impaired. Area at risk, evidenced with Evans blue, was 33.2 +/- 3.4% of the LV mass (LVM) in both groups, and mean infarct size, revealed by triphenyltetrazolium chloride (TTC), was 27.3 +/- 2.6% of the LVM in the BM-573-treated group (NS). Histological examination and immunohistochemical identification of desmin revealed necrosis in the anteroseptal region similar in both groups, while myocardial ATP dosages and electron microscopy also showed that BM-573 had no cardioprotective effect. Conclusions: These data suggest that BM-573 failed to prevent reperfusion injury in acutely ischemic pigs. (C) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailEvaluation of BRCA1-related molecular features and microRNAs as prognostic factors for triple negative breast cancers.
Boukerroucha, Meriem ULg; Josse, Claire ULg; El Guendi, Sonia ULg et al

in BMC cancer (2015), 15(1), 755

BACKGROUND: The BRCA1 gene plays a key role in triple negative breast cancers (TNBCs), in which its expression can be lost by multiple mechanisms: germinal mutation followed by deletion of the second ... [more ▼]

BACKGROUND: The BRCA1 gene plays a key role in triple negative breast cancers (TNBCs), in which its expression can be lost by multiple mechanisms: germinal mutation followed by deletion of the second allele; negative regulation by promoter methylation; or miRNA-mediated silencing. This study aimed to establish a correlation among the BRCA1-related molecular parameters, tumor characteristics and clinical follow-up of patients to find new prognostic factors. METHODS: BRCA1 protein and mRNA expression was quantified in situ in the TNBCs of 69 patients. BRCA1 promoter methylation status was checked, as well as cytokeratin 5/6 expression. Maintenance of expressed BRCA1 protein interaction with BARD1 was quantified, as a marker of BRCA1 functionality, and the tumor expression profiles of 27 microRNAs were determined. RESULTS: miR-548c-5p was emphasized as a new independent prognostic factor in TNBC. A combination of the tumoral expression of miR-548c and three other known prognostic parameters (tumor size, lymph node invasion and CK 5/6 expression status) allowed for relapse prediction by logistic regression with an area under the curve (AUC) = 0.96. BRCA1 mRNA and protein in situ expression, as well as the amount of BRCA1 ligated to BARD1 in the tumor, lacked any associations with patient outcomes, likely due to high intratumoral heterogeneity, and thus could not be used for clinical purposes. CONCLUSIONS: In situ BRCA1-related expression parameters could be used for clinical purposes at the time of diagnosis. In contrast, miR-548c-5p showed a promising potential as a prognostic factor in TNBC. [less ▲]

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See detailEvaluation of bromocriptine in the treatment of acute severe peripartum cardiomyopathy: a proof-of-concept pilot study.
Sliwa, Karen; Blauwet, Lori; Tibazarwa, Kemi et al

in Circulation (2010), 121(13), 1465-73

BACKGROUND: Peripartum cardiomyopathy (PPCM) is a potentially life-threatening heart disease that occurs in previously healthy women. We identified prolactin, mainly its 16-kDa angiostatic and ... [more ▼]

BACKGROUND: Peripartum cardiomyopathy (PPCM) is a potentially life-threatening heart disease that occurs in previously healthy women. We identified prolactin, mainly its 16-kDa angiostatic and proapoptotic form, as a key factor in PPCM pathophysiology. Previous reports suggest that bromocriptine may have beneficial effects in women with acute onset of PPCM. METHODS AND RESULTS: A prospective, single-center, randomized, open-label, proof-of-concept pilot study of women with newly diagnosed PPCM receiving standard care (PPCM-Std; n=10) versus standard care plus bromocriptine for 8 weeks (PPCM-Br, n=10) was conducted. Because mothers receiving bromocriptine could not breast-feed, the 6-month outcome of their children (n=21) was studied as a secondary end point. Blinded clinical, hemodynamic, and echocardiographic assessments were performed at baseline and 6 months after diagnosis. Cardiac magnetic resonance imaging was performed 4 to 6 weeks after diagnosis in PPCM-Br patients. There were no significant differences in baseline characteristics, including serum 16-kDa prolactin levels and cathepsin D activity, between the 2 study groups. PPCM-Br patients displayed greater recovery of left ventricular ejection fraction (27% to 58%; P=0.012) compared with PPCM-Std patients (27% to 36%) at 6 months. One patient in the PPCM-Br group died compared with 4 patients in the PPCM-Std group. Significantly fewer PPCM-Br patients (n=1, 10%) experienced the composite end point of poor outcome defined as death, New York Heart Association functional class III/IV, or left ventricular ejection fraction <35% at 6 months compared with the PPCM-Std patients (n=8, 80%; P=0.006). Cardiac magnetic resonance imaging revealed no intracavitary thrombi. Infants of mothers in both groups showed normal growth and survival. CONCLUSIONS: In this trial, the addition of bromocriptine to standard heart failure therapy appeared to improve left ventricular ejection fraction and a composite clinical outcome in women with acute severe PPCM, although the number of patients studied was small and the results cannot be considered definitive. Larger-scale multicenter and blinded studies are in progress to test this strategy more robustly. [less ▲]

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See detailEvaluation of Carabids as bioindicators : a case study in Belgium.
Dufrêne, Marc ULg; Baguette, Michel; Desender, Konjev et al

in Stork, Nigel (Ed.) The Role of Ground Beetles in Ecological and Environmental Studies (1990)

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See detailEvaluation of CellSolutions BestPrep(R) Automated Thin-Layer Liquid-Based Cytology Papanicolaou Slide Preparation and BestCyte(R) Cell Sorter Imaging System.
Delga, Agnes; GOFFIN, Frédéric ULg; Kridelka, Frédéric ULg et al

in Acta cytologica (2014)

Objective: A double-blind study was conducted to compare the performance of the new BestPrep(R) (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep(R) (Hologic). Study Design ... [more ▼]

Objective: A double-blind study was conducted to compare the performance of the new BestPrep(R) (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep(R) (Hologic). Study Design: Samples from the study patients (n = 105) were collected twice in the same encounter with the ThinPrep sample always taken first and the BestPrep sample collected second. Slides were prepared according to both manufacturers' protocols and evaluated using manual microscopic review and the BestCyte(R) cell sorter imaging system (CellSolutions). Diagnostic truth for each case was determined by independent manual review of both slides by multiple pathologists and histology when available. The presence of atypical squamous cells of undetermined significance was the threshold for positive for sensitivity and specificity calculations. Results: BestPrep and ThinPrep, by manual review, had sensitivities for high-grade squamous intraepithelial lesion (HSIL) cases of 100 and 95.6%, respectively. Using the BestCyte cell sorter, both had 100% sensitivity. For the same HSIL cases, the digene HC2 high-risk human papillomavirus DNA test had sensitivities of 100% (BestPrep) and 95.6% (ThinPrep). Specificities were 71.4% (BestPrep) and 54.8% (ThinPrep). Conclusions: BestPrep was equivalent to ThinPrep for manual review even though BestPrep was always the second sample collected. The BestCyte cell sorter provides a practical alternative to manual review for both BestPrep and ThinPrep slides. (c) 2014 S. Karger AG, Basel. [less ▲]

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See detailEvaluation of chicory seeds maturity by chlorophyll fluorescence imaging
Ooms, David ULg; Destain, Marie-France ULg

in Biosystems Engineering (2011), 110

Chicory (Cichorium intybus L.) seed production includes sorting to remove foreign materials and non-viable seeds. A machine vision system was developed to monitor the fluorescence in order to detect the ... [more ▼]

Chicory (Cichorium intybus L.) seed production includes sorting to remove foreign materials and non-viable seeds. A machine vision system was developed to monitor the fluorescence in order to detect the immature chicory seeds. It comprised a monochromatic light source, a highpass filter and a monochromatic CCD camera sensitive to red and infrared. With this device, blue light reflected by the seeds was blocked whilst red fluorescence was measured by the camera. A segmentation algorithm was designed to estimate separately the fluorescence intensities of the pappus, a crown of scales, and the main body of the pericarp. Experiments were carried out on five clones of cross-pollinated chicory plants used for seed production. Two hundred flower heads were labelled at flowering and harvested at different times during the maturation process expressed in “days after flowering” (DAF). Germination tests were performed according to the recommendations of the International Seed Testing Association to measure the germination percentage (GP) and the germination rate (GR), an indicator of seed vigour. Seed chlorophyll content diminished during maturation following a different logistic trend for the pappus and the pericarp. The GP increased from 18 DAF to reach its maximum value at 21 DAF, but the GR remained low until 30 DAF and increased afterwards. The potential of chlorophyll fluorescence to be used as an indicator of chicory seed vigour was the greatest between 21 and 36 DAF. [less ▲]

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See detailEvaluation of chlorine containing cellulose-based chiral stationary phases for the LC enantioseparation of basic pharmaceuticals using polar non-aqueous mobile phases.
Dossou, Katina Sourou Sylvestre ULg; CHIAP, Patrice ULg; Servais, Anne-Catherine ULg et al

in Journal of Separation Science (2011), 34(6), 617-22

The discrimination ability of three cellulose-based chiral stationary phases (CSPs) was evaluated towards the enantiomers of basic drugs, using ACN as the main solvent in polar organic mobile phases. The ... [more ▼]

The discrimination ability of three cellulose-based chiral stationary phases (CSPs) was evaluated towards the enantiomers of basic drugs, using ACN as the main solvent in polar organic mobile phases. The study was focused on CSPs containing cellulose tris(3-chloro-4-methylphenylcarbamate) (3-Cl-4-MePC), cellulose tris(4-chloro-3-methylphenylcarbamate) (4-Cl-3-MePC) or cellulose tris(3,5-dichlorophenylcarbamate) (3,5-diClPC) as the chiral selector. The behaviour of these CSPs was studied systematically in order to investigate the influence of the presence and position of the chlorine substituents on the phenylcarbamate moieties on the retention and resolution of the enantiomers. The evaluation was made with three different generic mobile phases, namely ACN/0.1%DEA/0.1% TFA (DEA, diethylamine), ACN/0.1%DEA/0.2% FA and ACN/0.1%DEA/0.2%AcA, deduced from the previous study. The nature of the acidic additive and of the chiral selector was found to be particularly important for the retention and enantioresolution of these basic compounds. High-resolution values could be obtained for most studied enantiomers with these CSPs, clearly demonstrating the interest of using them in combination with polar organic mobile phases. However, significant differences in enantioresolution between the CSPs have been observed for many compounds, indicating that these phases seem to be quite complementary. [less ▲]

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See detailEvaluation of circulating irisin levels in healthy young individuals after a single 100,000 IU vitamin D dose
CAVALIER, Etienne ULg; Mismetti, Valentine; Souberbielle, Jean-Claude

in Annales d'Endocrinologie (2014), 73(3), 162-164

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See detailEvaluation of classical NSAIDS and COX-2 selective inhibitors on purified ovine enzymes and human whole blood
De Leval, X; Dogne, JM; Delange, J et al

in Annals of the Rheumatic Diseases (2001), 60(S1), 343

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See detailEvaluation of classical NSAIDS and COX-2 selective inhibitors on purified ovine enzymes and human whole blood
de Leval, X; Dogne, JM; Delarge, J et al

in Clinical Rheumatology (2001), 20

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See detailEvaluation of classical NSAIDs and COX-2 selective inhibitors on purified ovine enzymes and human whole blood
De Leval, X.; Delarge, J.; Devel, P. et al

in Prostaglandins, Leukotrienes, and Essential Fatty Acids (2001), 64

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See detailEvaluation of classical NSAIDs in a model of human whole blood assay
De Leval, X.; Delarge, J.; Devel, P. et al

Poster (2000, March 04)

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See detailEvaluation of classical NSAIDs on COX-1 and COX-2 purified enzymes and prognosis of their effects on physiological responses
De Leval, X.; Henrotin, Yves ULg; Masereel, B. et al

Poster (1999, November 20)

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See detailEvaluation of classical NSAIDs on COX-1 and COX-2 purified enzymes and prognosis of their effects on physiological responses
De Leval, X.; Henrotin, Y.; Masereel, B. et al

in Fundamental & Clinical Pharmacology (2000), 14

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See detailEvaluation of classical NSAIDS on COX-1 and COX-2 purifies enzymes and prognosis of their effects on physiological responses.
De Leval, X.; Henrotin, Yves ULg; Masereel, B. et al

in Fundamental & Clinical Pharmacology (2003), 14

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See detailEvaluation of climate changes impact on the hydrological behaviour of the peaty mediums - Application to the Haute Lesse-Ourthe area (Belgium).
Sohier, Catherine ULg; Dautrebande, Sylvia; Degre, Aurore ULg

Poster (2010, April)

The peaty and wet mediums have a very important role to play in term of biodiversity conservation, hydrologic pattern regulation, fauna habitat and landscape value. In the Haute Lesse – Ourthe catchments ... [more ▼]

The peaty and wet mediums have a very important role to play in term of biodiversity conservation, hydrologic pattern regulation, fauna habitat and landscape value. In the Haute Lesse – Ourthe catchments (Saint-Hubert), numbers of these mediums were strongly modified by a drainage network. This drainage network was implemented to permit a forestry production focussed on the Spruce. It has severely disturbed the hydrological cycle. The study consists in modelling the impacts of restoration practices carried out notably within the framework of the LIFE European project on the hydrological behaviour of the peat bog. Then we modelled the effect of climate change. Physical properties and retention curve of peaty materials were characterized on the basis of undisturbed samples using the Richards’apparatus. Calculations were carried out with the hydrological model EPICgrid (GxABT). The model was initially validated on several reference catchments of the study zone. It was then applied at local scale for various peaty mediums (peat type, drainage characteristics, vegetation type) and for current and future climatic conditions. A regional simulation was finally carried out in order to evaluate the impacts of peat bogs restoration measures at catchment scale. Simulations highlighted the differences in physical and hydrous properties of the different peat types (“true” peat and “degraded” peat). Local and regional impacts of peat bogs restoration on their hydrological behaviour have been evaluated. Eventually, simulations allowed us to evaluate climate change’s impact on the hydrological behaviour of the peaty mediums. The model showed notably that future climatic conditions could generate severe droughts which can become critical in some situations. [less ▲]

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See detailEvaluation of computed tomographic anatomy of the equine metacarpophalangeal joint
Vanderperren, K.; Ghaye, Benoit ULg; Hoegaerts, M. et al

in American Journal of Veterinary Research (2008), 69(5), 631-638

OBJECTIVE: To determine the detailed computed tomography (CT) anatomy of the metacarpophalangeal (MCP) joint in healthy horses. SAMPLE POPULATION: 10 cadaveric forelimbs from 10 adult horses without ... [more ▼]

OBJECTIVE: To determine the detailed computed tomography (CT) anatomy of the metacarpophalangeal (MCP) joint in healthy horses. SAMPLE POPULATION: 10 cadaveric forelimbs from 10 adult horses without orthopedic disease. PROCEDURES: CT of the MCP joint was performed on 4 forelimbs. In 1 of the limbs, CT was also performed after intra-articular injection of 30 mL of contrast medium (40 mg of iodine/mL). Transverse slices 1-mm thick were obtained, and sagittal and dorsal planes were reformatted with a slice thickness of 2 mm. The CT images were matched with corresponding anatomic slices from 6 additional forelimbs. RESULTS: The third metacarpal bone, proximal sesamoid bones, and proximal phalanx could be clearly visualized. Common digital extensor tendon; accessory digital extensor tendon; lateral digital extensor tendon; superficial digital flexor tendon (including manica flexoria); deep digital flexor tendon; branches of the suspensory ligament (including its attachment); extensor branches of the suspensory ligament; collateral ligaments; straight, oblique, and cruciate distal sesamoidean ligaments; intersesamoidean ligament; annular ligament; and joint capsule could be seen. Collateral sesamoidean ligaments and short distal sesamoidean ligaments could be localized but not at all times clearly identified, whereas the metacarpointersesamoidean ligament could not be identified. The cartilage of the MCP joint could be assessed on the postcontrast sequence. CONCLUSIONS AND CLINICAL RELEVANCE: CT of the equine MCP joint can be of great value when results of radiography and ultrasonography are inconclusive. Images obtained in this study may serve as reference for CT of the equine MCP joint. [less ▲]

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